Citations for PNA Bio :
51 - 100 of 162
citations
since 2017
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... embryos at the very early 1 cell stage were injected with 374.5 ng/µl of the guide RNA targeted to zebrafish klc4 complexed with 800 ng/µl of Cas9 protein with NLS (PNA Bio). Injection volume was approximately 1nL ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA-protein complexes (RNPs) were prepared by mixing Cas9 protein 30-60 ug (PNA Bio) and 15-30 ug of gRNA per aliquot followed by incubation of 10 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... 1 μg cas9 (PNA Bio) and 500 ng of each sgRNA were incubated on ice for 20 minutes ...
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bioRxiv - Immunology 2020Quote: ... Cas9 protein (PNA bio) and gRNA were incubated at room temperature for 15 min in a 3:1 reaction with 15μg Cas9 and 5000 ng gRNA (2500ng of sgRNA#1 and 2500ng of sgRNA#2).47 The incubation product (gRNA and Cas9 complex ...
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bioRxiv - Genetics 2020Quote: ... lyophilized Cas9 protein (PNA Bio Inc, CP01) was reconstituted to a stock concentration of 1 μg/μl in 20 mM Hepes ...
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bioRxiv - Developmental Biology 2020Quote: ... esrp2 and irf6 CRISPR sgRNAs were generated by in vitro transcription from a SP6 promoter as described.(Gagnon et al., 2014) Lyophilized Cas9 protein (PNA Bio) was resuspended in ddH2O to a stock concentration of 1 μg/μl and stored in single-use aliquots in −80°C to avoid freeze-thaw inactivation and kept for 6 months ...
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bioRxiv - Genetics 2020, published in PLOS Genetics doi: 10.1371/journal.pgen.1008752Quote: ... Zygotes were electroporated according to the ZEN2 protocol (93, 94) with a final concentration of 250ng/ul Cas9 mRNA (PNA Bio) and 300ng/ul sgRNA dissolved in TE pH7.5/Opti-MEM at a 1:1 ratio (93) ...
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bioRxiv - Genetics 2020, published in PLOS Genetics doi: 10.1371/journal.pgen.1008752Quote: ... M4KO mice were generated by cytoplasmic microinjection of 25ng/ul Cas9 mRNA (PNA Bio) and 12.5ng/ul of each of four sgRNAs ...
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bioRxiv - Molecular Biology 2020Quote: ... 500 ng/μl rCas9 protein (PNA Bio CP01-20 Thousand Oaks, California) and duplex buffer (IDT ...
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bioRxiv - Molecular Biology 2020, published in Cell Cycle doi: 10.1080/15384101.2020.1849979Quote: ... the coverslips were dehydrated and incubated with a telomeric G-strand PNA probe (AlexaFluor 488-TTAGGG3; PNA Bio) in hybridization buffer (10 mM Tris pH 7.4 ...
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bioRxiv - Developmental Biology 2020, published in Development doi: 10.1242/dev.195008Quote: ... Cas9 (PNA Bio, CP01-50) ribonucleic particles (CRNPs ...
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bioRxiv - Developmental Biology 2020Quote: ... Cas9 protein was purchased from PNA Bio Inc ...
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bioRxiv - Genomics 2020Quote: ... 1 μM mPNA (PNA BIO INC, USA) and DNase/RNase free distilled water (10977-049 ...
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bioRxiv - Genomics 2020Quote: ... 1.25 μM cPNA (PNA BIO INC, USA), 1 μM mPNA (PNA BIO INC ...
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bioRxiv - Neuroscience 2020Quote: ... along with sgRNA (80 ng/μL) and Cas9 protein (300 ng/μL; PNA Bio, CP01-200). A total of 1500 ORL embryos were injected at the Insect Transformation Facility at the University of Maryland Institute for BioScience & Biotechnology ...
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bioRxiv - Developmental Biology 2020Quote: ... and general Reverse_5’-AAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCT ATTTCTAGCTCTAAAAC -3’ Embryos were injected with 1 ng Cas9 protein (PNA Bio) and 300 pg sgRNA at 1-cell stage and cultivated at room temperature until desired stage ...
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bioRxiv - Cell Biology 2020Quote: ... Injection mixture was prepared on ice containing 5 µM Cas9 (PNA Bio, CP02), 1 µg/µL sgRNA ...
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bioRxiv - Evolutionary Biology 2020Quote: ... sgRNAs were mixed with purified Cas9 protein (PNA Bio #CP01) with a final injected concentration of 0.05% phenol red to visualize the injection mix ...
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bioRxiv - Developmental Biology 2020Quote: ... Cas9 (1 mg/ml; CP01-200, PNA Bio Inc) and fresh sgRNA (1 ug ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Cas9 protein (PNA Bio #CP01), phenol red ...
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bioRxiv - Genetics 2020Quote: ... An injection mix was made with 40 ng/ul sgRNA RNA and 300 ng/ul Cas9 protein (PNA Bio # CP01-50) and injected into embryos from our BDC stock (Fig ...
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bioRxiv - Bioengineering 2020Quote: ... 150ng of Cas9 protein (PNA Bio, Inc., Newbury Park, CA), 1μL of 10X BSA ...
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bioRxiv - Microbiology 2020Quote: ... The reaction mixture was placed in the thermocycler for PCR settings prescribed by PNA Bio: 94° C for 3 minutes ...
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bioRxiv - Immunology 2020Quote: ... Purified sgRNA (0.5 μg) was incubated with Cas9 protein (1 μg, PNA Bio, Newbury Park, CA, USA) for 10 min at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Telomere FISH was performed using Alexa 488 or Cy3-OO-TelC labeled PNA probes (PNA Bio Inc.). Streptavidin−Alexa 488 (Invitrogen #S32354 ...
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bioRxiv - Molecular Biology 2020Quote: ... CO-FISH was performed as described67 using a TelC-Alexa488-conjugated PNA probe (PNA Bio F1004) followed by a TelG-Cy3-conjugated PNA probe (PNA Bio F1006) ...
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bioRxiv - Molecular Biology 2020Quote: ... Telomeres were detected using TelG-Alexa488-conjugated PNA probe (PNA Bio F1008) with a 1:25 dilution in hybridization solution (35% formamide ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by a TelG-Cy3-conjugated PNA probe (PNA Bio F1006). Images were captured using a Zeiss Spinning Disk confocal microscope ...
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bioRxiv - Molecular Biology 2020Quote: FISH was performed with TelC-Cy3 (PNA Bio F1002) and CENPB-Alexa488 (F3004 ...
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bioRxiv - Neuroscience 2020Quote: ... and donor vectors (generated by PNA Bio). The human TREM2 cDNA sequence was flanked on each side by 1-kb homology arms for the mTrem2 ...
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bioRxiv - Neuroscience 2020Quote: ... We directly mixed recombinant Cas9 protein (300 ng/µL; PNA Bio, CP01-200), sgRNA (80 ng/µL ...
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bioRxiv - Neuroscience 2020Quote: ... before adding recombinant Cas9 protein (300 ng/µL; PNA Bio, CP01-200) for embryo injection.
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bioRxiv - Neuroscience 2020Quote: ... each targeting a different gene (80 ng/uL each) with Cas9 protein (300 ng/µL; PNA Bio, CP01-200) and injected into ORL embryos ...
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bioRxiv - Developmental Biology 2020, published in EMBO reports doi: 10.15252/embr.202050612Quote: ... the protein Cas9 (recombinant cas protein from S. pyogenes PNA Bio CP01, final concentration 100 ng/μL) and KCL (final concentration 200 mM) ...
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bioRxiv - Bioengineering 2020Quote: ... 167ng/μL of Cas9 protein (PNA Bio, Inc., Newbury Park, CA) and 133ng/μL of donor plasmid ...
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bioRxiv - Bioengineering 2020Quote: ... Mutation rate for each guide was determined by laser-assisted cytoplasmic injection37 of in vitro fertilized embryos with 6pL of a solution containing 67ng/μL of in vitro transcribed gRNA alongside 133ng/μL of Cas9 mRNA or 167ng/μL of Cas9 protein (PNA Bio, Inc., Newbury Park, CA) incubated at room temperature for 30 minutes prior to injection ...
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bioRxiv - Neuroscience 2020Quote: ... donor plasmid (700 ng/µL) and Cas9 protein (300 ng/µL, PNA Bio #CP01-200) was injected into 1533 Orlando strain embryos.
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bioRxiv - Bioengineering 2020Quote: Anticoagulant activity of 2HF-2NN1 was reversed using ssDNA purchased from IDT and ssPNA (peptide-nucleic-acid) antidote strands purchased from PNA Bio. A solution containing two specific antidotes (500 μM ...
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bioRxiv - Bioengineering 2020Quote: ... Single-stranded PNA antidotes were ordered with HPLC purification from PNA Bio. CaCl2 solution and aPTT reagent were purchased from Trinity Biotech Plc ...
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bioRxiv - Cancer Biology 2020Quote: ... following the IF protocol coverslips were denatured and hybridized TRITC-OO[TTAGGG]3 labeled PNA probe (PNA Bio). Digital images were taken using Zeiss Axio Imager M1 ...
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bioRxiv - Cancer Biology 2020Quote: ... coverslips were hybridized with a TRITC-OO[TTAGGG]3 labeled PNA probe (PNA Bio) to detect telomeric DNA ...
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bioRxiv - Cancer Biology 2020Quote: ... Coverslips were dehydrated in an ethanol series (70%, 90% and 100%) and hybridized at RT with a TRITC-OO[TTAGGG]3 labeled PNA probe (PNA Bio) in hybridization buffer (70% formamide ...
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bioRxiv - Neuroscience 2020Quote: ... Cas9 protein was ordered from PNA Bio (CP01-50, Thousand Oaks, CA) and used to check cutting efficiency of the guides in vitro ...
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bioRxiv - Immunology 2020Quote: ... The sequence has been designed to have a melting temperature above 55°C (predicted with the PNA tool: https://www.pnabio.com/support/PNA_Tool.htm, from PNA Bio, Inc.) and orthogonal to the sequence of M13mp18 and validated using NCBI BLAST online tool38.
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bioRxiv - Bioengineering 2020Quote: ... purified SpyCas9 protein 30 pmol (PNA Bio, #CP02 or 3xNLS-SpCas943(prepared by the Scot Wolfe laboratory ...
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bioRxiv - Genomics 2020Quote: ... the same protocol was carried out using a centromere probe (CENPB-AF488, PNA Bio F3004).
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bioRxiv - Genomics 2020Quote: ... and dehydrated in an ethanol series before co-denaturation of slide and PNA probes (TelG-Cy3 PNA Bio F1006, CENPB-AF488 PNA Bio F3004) for 3 mins at 80°C in hybridization solution (10 mM Tris-HCl pH 7.2 ...
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bioRxiv - Genomics 2020Quote: ... and dehydrated in an ethanol series before co-denaturation of slide and PNA probes (TelG-Cy3 PNA Bio F1006, CENPB-AF488 PNA Bio F3004) for 3 mins at 80°C in hybridization solution (10 mM Tris-HCl pH 7.2 ...
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bioRxiv - Genomics 2020Quote: ... Slides were co-denatured for 2 mins at 80°C with TelG-647 (PNA Bio F1014) in hybridization solution (10mM Tris-HCl pH 7.2 ...
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bioRxiv - Bioengineering 2020Quote: Mutation rate for each guide was determined by laser-assisted cytoplasmic injection36 of in vitro fertilized embryos with 6pL of a solution containing 67ng/μL of in vitro transcribed gRNA alongside 133ng/μL of Cas9 mRNA or 167ng/μL of Cas9 protein (PNA Bio, Inc., Newbury Park, CA) incubated at room temperature for 30 minutes prior to injection ...