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Citations for PNA Bio :
1 - 50 of 201 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... 2400 ng Cas9 protein with NLS (PNA Bio CP01) were combined with 1200 ng of sgRNA in 4.5 μl nuclease-free water on the day of injection and left at room temperature for 10 minutes to form ribonucleoprotein complexes ...
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bioRxiv - Developmental Biology 2024Quote: ... co-injected one-cell stage embryos with RNPs containing 25 pg of gRNA and 500 pg of NLS-Cas9 protein (PNA Bio, Thousand Oaks, CA USA), and raised these fish to adulthood ...
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bioRxiv - Cancer Biology 2024Quote: ... The telomere PNA probe (TelC) was purchased from PNA Bio (F1004). Images were collected by using an Axio Observer.Z1/7 (Zeiss Microimaging GmbH ...
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bioRxiv - Plant Biology 2024Quote: ... 0.19uL of 100uM mitochondrial PNA (GGCAAGTGTTCTTCGGA; PNA Bio, Thousand Oaks ...
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bioRxiv - Plant Biology 2024Quote: ... 0.19uL of 100uM chloroplast PNA (GGCTCAACCCTGGACAG; PNA Bio, Thousand Oaks ...
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bioRxiv - Neuroscience 2024Quote: ... 25ng/μL gRNA2 and 50ng/uL SpCas9 protein (PNA Bio) was microinjected into the pronucleus of C57BL/6 zygotes ...
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bioRxiv - Neuroscience 2024Quote: ... 25ng/μL gRNA1 and 50ng/μL SpCas9 protein (PNA Bio) was microinjected into the pronucleus of zygotes positive for 3’ LoxP site ...
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bioRxiv - Biochemistry 2024Quote: ... 5 µl of injection mixture consist of 1 µg of recombinant nls-Cas9 (PNA Bio), 200 ng of purified sgRNA ...
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bioRxiv - Genetics 2024Quote: ... elegans were injected with nuclear-localized Cas9 (PNA Bio) preincubated at 37°C for 10 min with a hybridized crRNA/tracrRNA (Integrated DNA Technologies) ...
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bioRxiv - Developmental Biology 2024Quote: ... 1200 ng gRNA were mixed with 2400 ng Cas9 protein with NLS (PNA Bio) in 5 μl nuclease-free water and incubated for 10 minutes at room temperature to form ribonucleoprotein (RNP ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL of annealed RNAs was incubated with 1 µL of 10 mg/mL Cas9 protein (PNA Bio # CP01-200) at room temperature (22°C ...
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bioRxiv - Neuroscience 2023Quote: ... complexed with Cas9 protein (PNA Bio), and injected into 1-cell stage embryos ...
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bioRxiv - Developmental Biology 2023Quote: ... but later studies utilized Cas9 protein (CP01, PNA BIO INC). When Cas9 protein was used we incubated the Cas9 protein/sgRNA mixture at 37°C for 5 minutes before injection ...
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bioRxiv - Cell Biology 2023Quote: ... and 1.65µL of each of two lmn-1 crRNA per strain (30pmol/µl) was combined and added to 1µL of purified Cas9 protein (5µg/µl from PNA Bio, Inc). Following addition of 1µL of the dpy-10 ssODN (500ng/µL ...
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bioRxiv - Molecular Biology 2023Quote: ... Telomeric PNA probe was diluted 1:100 (PNA Bio, F1004, TelC-Alexa 488, 3xCCCTAA) in hybridization buffer (70% formamide ...
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bioRxiv - Synthetic Biology 2023Quote: ... CPP-PNAs were handled per guidance from PNA Bio: lyophilized pellets were thawed to room temperature ...
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bioRxiv - Synthetic Biology 2023Quote: PNAs conjugated to the CPP (KFF)3K were ordered from PNA Bio Inc ...
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bioRxiv - Cancer Biology 2023Quote: ... and 0.5% blocking solution (Roche)] containing 100nM telomeric PNA probe TelC-FITC (F1009, PNA Bio), and hybridization was continued for 2 h at room temperature in the dark moisturized chambers ...
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bioRxiv - Neuroscience 2023Quote: ... 60 pg sgRNA and 100 pg Cas9 protein (PNA Bio) were co-injected into one-cell stage wild-type embryos ...
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bioRxiv - Microbiology 2023Quote: ... For HIV DNA probe hybridization tissues were incubated with 10 µM PNA DNA probe for Nef-PNA Alexa Fluor 488 and Alu-PNA Cy5 (PNA Bio). Next ...
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bioRxiv - Developmental Biology 2023Quote: ... the synthetized sgRNA was incubated with Cas9 protein (PNA Bio, Cat# CP02-250) to form an RNP complex ...
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bioRxiv - Synthetic Biology 2023Quote: ... The injection master mix also included preassembled RNP complex of Cas9 protein (200 ng/ml) (PNA Bio)33 ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated with a commercially available Cy3-labeled (CCCTAA)3 peptide nucleic acid probe (PNA Bio) recognizing mammalian telomere sequences ...
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bioRxiv - Genetics 2023Quote: ... 40 ng/ul each of the two gRNAs together with 333 ng/ul Cas9 (PNA Bio) was injected into Thai strain embryos by the University of Maryland Insect Transformation Facility (https://www.ibbr.umd.edu/facilities/itf) ...
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bioRxiv - Neuroscience 2023Quote: ... The CRISPR injection mixture contained 300 ng/µl recombinant Cas9 protein (PNA Bio CP01) and 40 ng/ µl sgRNA (per guide ...
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bioRxiv - Molecular Biology 2023Quote: ... Cas9 (1 mg/ml; CP01-200, PNA Bio Inc) and fresh sgRNA (1 ug ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and 100 ng of which was used in cleavage assays with 300 ng of recombinant Cas9 (PNA BIO, Thousand Oaks, CA) and 100 ng of each guide RNA upon incubation at 37°C for 1 hour (Figure S5C).
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bioRxiv - Bioengineering 2023Quote: ... The Cas9 protein powder was purchased from PNA BIO Inc ...
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bioRxiv - Cell Biology 2023Quote: ... Fertilized eggs of C57BL/6J mice were microinjected with recombinant Cas9 (PNA Bio) and guide RNAs (Synthego ...
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bioRxiv - Cancer Biology 2023Quote: ... and 0.5% blocking solution (Roche)] containing 100nM telomeric PNA probe TelC-FITC (F1009, PNA Bio), and hybridization was continued at RT for 2 h in the dark moisturized chambers ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we used a non-homologous end joining mediated strategy by injecting the mixture of Cas9 protein (#CP01; PNA Bio) and sgRNAs into the embryos of this species ...
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bioRxiv - Cell Biology 2023Quote: ... RNP complexes consisting of 4 µM sgRNA (IDT or Synthego) and 3 µM SpyCas9 protein (PNA Bio) were prepared in Buffer R provided by the Neon Transfection System Kit to a volume of 6 µl ...
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bioRxiv - Cell Biology 2023Quote: ... Probes used for centromere 7 FISH was a Satellite Enumeration Probe from Cytocell (Cat# LPE 007G-A) and for CENP-B box FISH was a PNA probe from PNA Bio with the sequence 5’-ATTCGTTGGAAACGGGA-3’ ...
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bioRxiv - Microbiology 2023Quote: ... PNA blocker was designed and synthesised (PNA Bio, USA). The PCR was performed with 1 µM of each primer (Table S1) ...
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bioRxiv - Genetics 2023Quote: ... and Cas9 protein (PNA bio). A single gRNA with validated cleavage activity was chosen for incorporation into a synthetic gBlock (IDT ...
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bioRxiv - Cancer Biology 2023Quote: ... Purified sgRNA (0.5 µg) was incubated with Cas9 protein (1 µg, PNA Bio) for 10 min at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... 125 ng of each gRNA was separately incubated with 250 ng of Cas9 protein (PNA Bio Inc) for 10 minutes at room temp ...
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bioRxiv - Cell Biology 2023Quote: ... 20 μg of purified recombinant SpCas9 protein (PNA Bio, Inc., Thousand Oaks, CA, USA) was pre-complexed on ice for 20 min with 10 μg of the chemically modified sgRNA (bearing 2’-O methyl phosphorothioate- modified nucleotides at the first 3 and last 3 positions of the synthetic sgRNA ...
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bioRxiv - Molecular Biology 2023Quote: ... The PNA probe against the MSH3-targeting antisense strand was ordered from PNA Bio (Thousand Oaks, CA, USA) with the sequence (5’-3’ ...
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bioRxiv - Cell Biology 2023Quote: ... CD33 chemically modified guide RNAs (6.25 μg/106 cells, Synthego, Redwood City, CA) and Cas9 protein (12.5 μg/106 cells, PNA Bio, Thousand Oaks, CA) were mixed for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... 1.25 µL of mPNA blocker (5 µM; mitochondria blockers; PNA Bio), 1.25 µL of pPNA blocker (5 µM ...
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bioRxiv - Molecular Biology 2023Quote: ... or TelG-Cy3 PNA telomere probe together with a CENPB-Cy3 centromere probe (PNA Bio Inc). Slides were counterstained with DAPI (Electron Microscopy Sci. ...
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bioRxiv - Neuroscience 2023Quote: ... Fertilised eggs from C57BL/6J mice were injected with the two gRNAs (Msh3 gRNA 51/57 and Msh3 gRNA 89/69) and Cas9 protein (PNA BIO INC) and transferred to pseudopregnant CD1 female mice ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Cas9 recombinant protein with nuclear localization signal (260 ng/μl; PNA Bio, USA) was co-injected with the gRNA (140ng/µl ...
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bioRxiv - Genetics 2023Quote: ... The Cas9 protein was purchased from PNA Bio (CP01). All homozygous animals edited by CRISPR/Cas9 were validated by Sanger sequencing ...
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bioRxiv - Genomics 2023Quote: ... Centromeres were labeled with the pan-centromeric probe CENP-B-Cy5 (PNA Bio, F3005). After DNA FISH and CENP-B probe labeling ...
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bioRxiv - Molecular Biology 2023Quote: ... FAM-tagged TelG probe (PNA Bio F1005) was diluted (1:100 ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotin-tagged TelC probe (PNA Bio F2001) was diluted (1:200 ...
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bioRxiv - Cancer Biology 2023Quote: ... Chromosomes were hybridized to Tel G 488 PNA probe (PNA Bio) and washed in PNA Wash Buffer A before hybridization with Tec C Cy3 PNA probe (PNA Bio ...
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bioRxiv - Cancer Biology 2023Quote: ... Chromosomes were incubated in Tel C Cy 3 PNA probe (PNA Bio) at 83 °C for 5 minutes and allowed to hybridize at room temperature in a dark hybridization chamber for 2 hours at room temperature ...