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Citations for BioLegend :
3501 - 3550 of 3701 citations for Marker 7 Pcb Mixture W Pcb 118 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: 96-well flat-bottomed plates were pre-coated overnight at 4°C with 5 µg/ml anti-NKp30 (clone P30-15, Biolegend) and -CD18 (clone IB4 ...
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bioRxiv - Immunology 2021Quote: IFNAR1 on WT and Mt3-/- BMDMϕ was neutralized using 10 μg/ml monoclonal anti-IFNAR1 antibody (BioLegend; Clone: MAR1-5A3) 1h prior and 24h after iLPS (10 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... On day 6 BMDM were replated at 1 × 106 cells per well in 24-well plates and stimulated with 10 µg/ml Imiquimod (Biolegend) with 10 µg/ml anti-CD200R1 (OX131 ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed three times in ice cold blocking buffer and re-suspended in blocking buffer containing 0.1 μg/ml DAPI (Biolegend, USA).
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bioRxiv - Immunology 2022Quote: ... The BM cells per animal were spined down and then resuspended in 1 mL Red Blood Cell Lysis (RBCL) buffer (Biolegend) at room temperature for 1 min to lyse the red blood cells ...
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bioRxiv - Immunology 2022Quote: Splenic T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated as described above and stimulated with 5 μg/mL plate-bound αCD3 (Biolegend) and 100 U/mL IL-2 (Peprotech ...
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bioRxiv - Immunology 2022Quote: Splenic T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated as described above and stimulated with 5 μg/mL plate-bound αCD3 (Biolegend) and 100 U/mL IL-2 (Peprotech ...
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bioRxiv - Immunology 2022Quote: ... BMDCs were pre-incubated for 20 minutes at 37 °C with anti-mouse IFNAR1 (clone MAR1-5A3, 10 μg/mL, Biolegend), anti-mouse IL-12p40 or anti-mouse IL-12p70 Abs or an isotype control before stimulation.
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bioRxiv - Immunology 2022Quote: ... Sorted 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11, BioLegend), soluble 1 μg/mL anti-mouse CD28 mAb (clone 37.51 ...
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bioRxiv - Molecular Biology 2019Quote: ... for 3 min and stained using mouse anti-human HER2 antibody conjugated to FITC (20 μg/ml, clone 24D2, BioLegend). Cells were incubated with anti-HER2 antibody for 20 min at 4°C in the dark and washed once with PBS supplemented with 1% FCS prior to the FACS analysis ...
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bioRxiv - Immunology 2019Quote: Enriched CD8+ were co-cultured with THP1 cells loaded with fixed E.coli at 30 BpC in the presence or absence of 20ug/mL LEAF™ anti-MR1 Antibody (Biolegend). Supernatants were collected at 72 hours ...
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bioRxiv - Immunology 2020Quote: ... single cell suspension of lung cells were washed and resuspended at 20 ×106 cells/mL in PBS + Zombie NIR Live/Dead stain (Biolegend) at 2μL/mL and incubated for 20 min at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... Unbound cells were collected and cultured for 24 hours in the presence of 1 μg/ml anti-mouse CD3ε (145-2C11, BioLegend) and 0.5 μg/ml CD28 (37.51 ...
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bioRxiv - Neuroscience 2019Quote: ... followed by overnight incubation at 4°C with either anti-beta amyloid 6E10 (mouse, 1:1,000 dilution of 1mg/mL stock) (Biolegend, USA), anti-tau MC1 (mouse ...
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bioRxiv - Immunology 2020Quote: ... Cells were then stained with 2 μg/mL of Alexa Fluor 647 labelled anti-CD5 antibody or anti-CD180 antibody (BioLegend) and 2 μg/mL of Alexa Fluor 488 labelled anti-IgM Fab fragment (Jackson ImmunoResearch ...
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bioRxiv - Synthetic Biology 2021Quote: ... incubated at a concentration of 0.1 µg/mL in HF for 3 minutes at room temperature or Zombie NIRTM (BioLegend, 423106) incubated in PBS at a 1:500 dilution for 15 minutes at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... cultured with WKYMVm for 24 h were collected in 1.5 ml Ep tubes after washing twice and were incubated with Alexa Fluor-488 (AF488, BioLegend, USA)-conjugated anti-mouse CD206 and activated protein C (APC ...
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bioRxiv - Cancer Biology 2020Quote: ... Single tumor cells were resuspended to 5 × 107 cell/mL and stained with CD298-APC (10 µL/106 cells)(BioLegend) and Sytox Green (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: ... The virus particles were stained at a concentration of 5×108 particles mL−1 and labeled with anti-GFP PE antibody (Clone FM264G, BioLegend) at 0.4 μg mL−1 in a 100 μL staining volume for a minimum of 30 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... T-25 or T-75 flasks were coated with 5 µg/mL anti-CD3ε antibodies (clone 145-2C11; 100340, BioLegend) in phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2021Quote: ... Heparinized blood diluted 1:1 in PBS overlaid onto Lymphopure density gradient medium 1.077 g/mL (BioLegend, San Diego, USA) in Leucosep tubes (Greiner Bio-One ...
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bioRxiv - Immunology 2021Quote: ... sort-purified γδ or αβ IELs were seeded at approximately 400K cells per well in round-bottom 96-well plates coated overnight with 2 μg/ml anti-CD3 (145-2C11, BioLegend), 2 μg/ml polyclonal Armenian hamster IgG (BioXCell) ...
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bioRxiv - Immunology 2021Quote: ... from Miltenyi according to the manufacturer’s instructions and cultured in the above medium supplemented with 2 µg/ml anti-human RP105 antibody clone (312907, BioLegend).
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bioRxiv - Immunology 2021Quote: ... isotype controls and inhibitor of VLA-4 were used at 1 μg/ml: LEAF™ purified anti-CD29 (clone HMβ1-1, 102209, BioLegend), LEAF™ purified anti-CD31 (clone MEC13.3 ...
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bioRxiv - Immunology 2021Quote: ... or total combined splenocytes plus bone marrow from WT or c-Rel-/- mice were stimulated for 48 hours in complete RPMI containing 10% fetal bovine serum (FBS) and 1% pen strep with either rmIL-2 (1000 U/ mL; BioLegend), rmIL-15 (100 ng/mL ...
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bioRxiv - Microbiology 2021Quote: ... for 15 min at 4°C prior to wash in 1 ml PBS and centrifugation at 1600 rpm for 5 min and surface molecule staining with CD206 APCCy7 (BioLegend), and intracellular staining of anti-human Arginase1 FITC (Ref ...
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bioRxiv - Immunology 2020Quote: ... lymph nodes were removed from Myc-eGFP mice and mashed and cell suspension activated with anti-CD3 (1 µg/mL, clone 145-2C11, Biolegend) and anti-CD28 (3 µg/mL ...
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bioRxiv - Immunology 2021Quote: ... at 4°C overnight with 70 µl/well of 1 µg/ml of a relevant capture mAb: purified anti-human IFN-γ (Clone:NIB42, BioLegend); Purified anti-human TNF-α (Clone:MAb1 ...
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bioRxiv - Cell Biology 2021Quote: ... at a final concentration of 2.5μg/ml and we run in parallel samples with IgG isotype control (BioLegend, Cat#400526). All antibodies were centrifuged for 10 min at 14,000 x g before use ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in FACS buffer (1% BSA in PBS) at a concentration of 108 cells/mL and 1 μL negative CD8+ T cell biotin-antibody cocktail (Biolegend)/million cells was added and allowed to incubate for 15 min on ice ...
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bioRxiv - Genomics 2022Quote: ... flat bottom 96-well plates were coated with 2 μg/mL of anti-CD3 (Clone: 145-2C11, BioLegend, Cat#100302) in PBS overnight at 4°C or 4 hrs at 37°C ...
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bioRxiv - Immunology 2022Quote: Cells isolated from murine livers were stained with 30 μg/mL Hoechst 33342 (Miltenyi) and Zombie NIR viability dye (diluted 1:250 in PBS; Biolegend) before FACS sorting (Sony SH800 ...
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bioRxiv - Immunology 2022Quote: ... M1 cells were generated by addition of 20ng/mL of granulocyte-macrophage colony-stimulating factor (GM-CSF, Cat. No. 572904, BioLegend) and M2 macrophages with 20ng/mL of macrophage –colony-stimulating factor (M-CSF ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T-cells were cultured alone or co-cultured with either of the B-cell populations at a ratio of 3:1 for 60 h at 37°C and 5% CO2 on anti-CD3 (2 μg/mL) (ULTRA-LEAF Biolegend) coated flat bottomed 384 CellCarrier Ultra microplates (PerkinElmer ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 million cells were resuspended in 1 mL of FACS buffer and 50 μL of Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) was added and left to bind the cells on ice for 15 minutes ...
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bioRxiv - Physiology 2022Quote: ... CD11b+-SVF were incubated with were with 0.2 mg/mL of anti-mouse PE-F4/80 (BioLegend, #12-4801-82) with utilization of PE rat IgG2a K isotype Control (BioLegend#12-4321-80 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were restimulated at 1×106 cells/mL concentration for 48 h on day 8-10 with 1 µg/mL plate-bound anti-CD3ε antibody (clone UCHT1, Biolegend). BiTE-mediated killing assays were carried out on day 14-16 of culture.
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bioRxiv - Immunology 2024Quote: ... CD4 conv T cells were resuspended in complete RPMI 10% FBS and incubated for 1 hour at 37C with 1 ug/ml of anti-mouse CD3 (2c11 clone; Biolegend) or left untreated ...
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bioRxiv - Immunology 2024Quote: ... Mice CD4+ T cells (4x105/well in 96-well plate) were activated with plate-coated αCD3 (5 µg/ml, Biolegend) and αCD28 (10 µg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were immunolabeled in blocking solution for 1 hour at RT with antibodies against the following proteins: ALIX at 5 μg/ml (mouse; no. 634502, Biolegend); IST1 at 0.4 μg/ml (rabbit ...
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bioRxiv - Immunology 2023Quote: ... 106 of the CTV labeled T cells were stimulated in vitro for 3 or 5 days in cultures containing 100ng/ml IL-15 (BioLegend) or a combination of 100ng/ml IL-7 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... or gE overlapping peptides (10 μg/mL, 153 peptides, 15mers with 11 aa overlap) in the presence of brefeldin A (BFA, Biolegend) for 8 hours or 16 hours at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... for 40 min on ice or with biotinylated anti-MR1 antibody 26.5 (BioLegend, custom conjugation, used at 5 μg/ml) for 40 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... in the presence of S2 mAbs (5 μg/mL), protein transport inhibitor cocktail (1:500, eBioscience) and anti-CD107a APC (clone H4A3, BioLegend). Antibody-dependent NK cell activation was allowed to occur for 4 hours at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2024Quote: γδ IELs were cultured ex vivo as previously described (33) in which 100,000 γδ IELs were activated with 1 ÿg/mL anti-CD3 (2C11, BioLegend) in the presence of 10 U/mL IL-2 ...
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bioRxiv - Cancer Biology 2024Quote: ... mesothelioma cell lines were further pre-treated with anti-MHC class I antibody (10 μg/ml) (Clone W6/32, Biolegend) for 1 hour ...
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bioRxiv - Bioengineering 2024Quote: T-cells were cultured in RPMI-1640 media supplemented with 5 µg/mL brefeldin A (BioLegend, San Diego, CA, USA). After 24 hours of cultivation ...
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bioRxiv - Cell Biology 2023Quote: ... Cell surface expression level of syndecan-1 was evaluated by incubating cells with anti-syndecan-1 antibody (10 µg/ml, B280312, Biolegend) for 1h at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Following combinations of cytokines and antibodies were used for the differentiation of T cells: for Th1 cells - 1 µg/ml anti-IL-4 (clone 11B11; Biolegend), 30 U/ml rhIL-2 (NIH) ...
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bioRxiv - Immunology 2023Quote: ... the appropriate number of wells of a flat-bottomed tissue culture plate were coated with 10 μg/mL αCD3 (Biolegend) in PBS for 3h at 37°C ...