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Citations for BioLegend :
3251 - 3300 of 3701 citations for Marker 7 Pcb Mixture W Pcb 118 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... mixed to a final concentration of 2 µg/mL or 60 kDa FITC-labeled streptavidin (Biolegend) to a final concentration of 50 µg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... red blood cells (RBCs) were removed by lysis in 2 mL of RBC lysis buffer (BioLegend) for 3 minutes ...
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bioRxiv - Immunology 2022Quote: ... slices were incubated with 20 μg/ml anti-CD45 (Pac-Blue-αCD45 Rat IgG2a, κ, BioLegend). Slices were washed 3x with 1X PBS for 10 min per wash.
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bioRxiv - Immunology 2022Quote: ... Colon cells were either untreated or treated with 25 µg/ml recombinant mouse IFN-β1 (Biolegend.) Supernatant was collected for ELISA and cells were stained for flow.
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bioRxiv - Immunology 2023Quote: ... 96-well plates were pre-coated with 5 μg/ml anti-hCD3 (BioLegend, Cat# 317326, RRID:AB_11150592), 1 μg/ml anti-hCD28 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then resuspended in FACS buffer with 1 μg/ml propidium iodide (PI) (BioLegend, 421301). FACS sorting was performed on a BD FACS Aria II sorter using a 100-µm nozzle ...
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bioRxiv - Immunology 2023Quote: ... or its control isotype Ultra-LEAF™ purified mouse IgG2a antibody (10μg/ml, MOPC-173, Biolegend). For single TIM-3 blockade (αTIM-3) ...
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bioRxiv - Immunology 2023Quote: ... or its respective isotype Ultra-LEAF™ purified mouse IgG1 antibody (10μg/ml, MOPC-21, Biolegend). Finally ...
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bioRxiv - Immunology 2023Quote: ... 96 well plates were coated with purified anti-mouse IgE (2ug/ml, Biolegend, Clone: RME-1) in 0.05M carbonate/ bicarbonate buffer and incubated overnight at 47C ...
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bioRxiv - Immunology 2023Quote: ... The cell pellet containing residual buffer was vortexed and then 1 mL TruePhos Fixation Buffer (Biolegend) was added drop-by-drop while under constant vortexing ...
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bioRxiv - Immunology 2023Quote: ... T cells were activated with 1 μg/ml plate-bound anti-CD3 (BioLegend, clone 145-2C11) and 1 μg/mL anti-CD28 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... cells were first stimulated for 5 hours with either anti-CD3 antibodies (1 ug/mL, Biolegend) or MTB300 peptide megapool (21 ...
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bioRxiv - Immunology 2023Quote: ... DC2.4 cells were then treated with 5ng/ml Recombinant Mouse IFN-ψ (Biolegend, Cat. No:575302) for 24 hours ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with 2 ug/ml PE/Cyanine7-conjugated anti-mouse TLR4 antibodies (BioLegend, 145407) or 2 ug/ml PE-conjugated anti-mouse TLR2 antibodies (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Cultures were treated with anti-human CD2 mAb (10µg/mL; Cat# 300240; Clone: RPA-2.10; Biolegend) or IgG (10 µg/mL ...
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bioRxiv - Immunology 2024Quote: ... LN or spleen single cell suspensions were activated with 0.5 µg/mL anti-mouse CD3 (Biolegend) and 0.5 µg/mL anti-mouse CD28 (eBioscience ...
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bioRxiv - Immunology 2024Quote: ... 10 µg/ml of Ultra-LEAFTM purified anti-mouse IL-4 antibody (clone 11B11, Biolegend, #504122), 10 ng/ml animal-free recombinant murine IFNγ (PeproTech ...
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bioRxiv - Neuroscience 2024Quote: ... Supernatant was collected and 10ul of 1mg/mL anti-HA antibody was added (Biolegend Clone 16B12). Samples were placed at 4C on a rotator for four hours ...
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bioRxiv - Immunology 2024Quote: ... and then stained 1h in PBS 2% BSA + 10µg/mL 2.4G2 with anti-vimentin AF647 (Biolegend) and counterstained using 500ng/mL DAPI dilactate (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... 10 µg/ml of Ultra-LEAFTM purified anti-mouse IL-17A antibody (clone QA20A33, Biolegend, # 946504), 10 µg/ml of Ultra-LEAFTM purified anti-mouse IL-4 antibody (clone 11B11 ...
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bioRxiv - Cell Biology 2020Quote: ... Surface marker staining was performed for 30 min on ice using CD34-eFluor 660 (eBioscience, clone RAM34, 1:100) and ITGA6-pacific blue (Biolegend, clone GoH3, 1:200). Cells were washed with PBS and stained with annexin V-AF488 (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were washed with FACS buffer and surface marker staining was performed for 30 min on ice using CD34-eFluor 660 (eBioscience, clone RAM34, 1:100) and ITGA6-PE/Cy7 (Biolegend, clone GoH3, 1:1000). The cells were washed with 1 % BSA in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Human tissues were incubated for 25 min at 4 °C with the following primary antibodies: CD24-PE (BioLegend, clone ML5, Cat, #: 311105, 1:100), CD44-FITC (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: ... Surface marker staining was performed for 30 min on ice with the following antibodies: CD34-eFluor 660 (eBioscience, clone RAM34, 1:100) and ITGA6-PE/Cy7 (Biolegend, clone GoH3, 1:1000) for sorting or CD34-eFluor 660 (eBioscience ...
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bioRxiv - Immunology 2022Quote: Antibody staining was done in the dark for 30 minutes at 4°C in 30 μL of FACS buffer with antibodies and anti-CD16/32 (TruStain FcX™ PLUS; BioLegend, #156604; 1:100). Acquisition was done on a BD FACSCanto™ II or BD LSRFortessa (both BD Biosciences) ...
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bioRxiv - Neuroscience 2022Quote: ... APC-Cy7 rat anti-mCD45 (1:200, Becton Dickinson, Franklin Lakes, NJ, USA) and APC-Cy7 anti-mTer119 (1:100, Biolegend, San Diego, CA, USA). In order to sort neural stem and precursor cells ...
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bioRxiv - Neuroscience 2023Quote: ... were blocked with 5% BSA and 5% goat serum in PBST (PBS/0.25% Triton X-100) for 30 min and then incubated with monoclonal 6E10 antibody (BioLegend, cat# 803001, 1:1000 dilution) overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed 3 x with 1% BSA before incubating for 1 h in anti-human IgG secondary antibody labelled with either APC (BioLegend, #410712, dilution 1:100) or Phycoerythrin (Abcam ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Isolated aortic single cells were stained with Fc receptor blocker (TruStain FcX anti-mouse CD16/32; 101319; BioLegend, San Diego, CA, USA, 1:100) for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were permeabilized with 0.1% Triton X-100 in PBS (5 min at RT) and immunolabeled with anti-HA (Biolegend, 901501; 1:500 in PBS) for 1 hr at RT ...
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bioRxiv - Genetics 2023Quote: ... The barcoded cells were then collected into a single tube and stained with a cocktail of antibodies: CD33 PECy7 (5 µL per 100 µL stain, Biolegend, cat#303434, clone WM53) and pSTAT3 AlexFlour488 (2.5 µL per 100 µL stain ...
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bioRxiv - Immunology 2023Quote: ... the cells were stained for 20 minutes at 4 °C using a Cyto-Fast™ Fix/Perm Buffer Set (426803, BioLegend; 100 μL/tube). After being washed and resuspended with Cyto-Fast™ perm wash solution ...
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bioRxiv - Neuroscience 2020Quote: ... anti-neurofilament to a phosphorylated heavy chain neurofilament (NF-H; mouse anti-human monoclonal antibody SMI-34, dilution 1:100, epitope retrieval time 10 min, Biolegend 835503; Biolegend, San Diego, CA). Hemosiderin deposition was evaluated on a section with only counterstain ...
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bioRxiv - Immunology 2020Quote: ... 10mM HEPES),and stained at 4°C for 25 minutes using the following cell surface antibodies: CD14 Pacific Blue (clone M5E2, BioLegend cat# 301815, 2:100 dilution); CD19 Pacific Blue (clone H1B19 ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were pelleted and resuspended in FACS buffer for staining with the following antibodies for 20 min at 4° C: Ter119-FITC (BioLegend, Catalog No. 116205, 1/100), EpCAM-PE (BioLegend ...
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bioRxiv - Systems Biology 2023Quote: ... were used at a concentration of 1:100 then stained with anti-rabbit-PE or anti-mouse Pacific Blue secondary antibody (BioLegend® #406421, Life Technologies #P10993) for flow cytometry.
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bioRxiv - Immunology 2024Quote: ... Yeast cells were washed two times with PBSF and subsequently stained for 30 minutes on ice with a cocktail of anti-HA APC antibody (Biolegend, Clone: 16B12, dilution 1:100), goat F(ab′)2 anti-human IgG PE (SouthernBiotech ...
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bioRxiv - Neuroscience 2021Quote: ... Alexa Fluor 594 or Alexa Fluor 647 conjugated anti-mouse CD31 (2 μg/mL; clone MEC13.3; BioLegend) was used ...
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bioRxiv - Immunology 2022Quote: ... media was supplemented with 2 μg/ml of mouse IgG1 K anti-human PD-1 antibody (Biolegend) either alone or in combination with 1 μg/ml mouse IgG2 B anti-human TIGIT (R&D systems ...
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bioRxiv - Microbiology 2019Quote: ... Sputum samples were also stained with 1 µg/ml of PE anti-human CD45RO in PBS (BioLegend) for 30 min to stain activated and memory T cells ...
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bioRxiv - Immunology 2019Quote: ... and the following day were restimulated with either 1 μg/ml each of anti-CD3 (HIT3α, Biolegend) or PMA and ionomycin (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... then incubated with 0.5 ml of human TruStain FcX Fc receptor blocking solution (Biolegend, San Diego, CA) for 10 minutes.
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bioRxiv - Microbiology 2020Quote: ... or SIV Gag or Env peptides (1 μg/mL) for 1 hour with CD107a PECy5 (eBioH4A3, BioLegend) in R10 media before adding 1 mg/mL of Brefeldin A (Sigma-Aldrich®) ...
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bioRxiv - Microbiology 2020Quote: ... Primary detection of TRX-tagged YpTop1 was with primary murine monoclonal anti-TRX (BioLegend #658902, 1μg/ml), ...
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bioRxiv - Cancer Biology 2020Quote: ... we incubated the nanowell array with 4 µg/ml PE anti-CD63 antibody (BioLegend, clone NVG-2) for 45 minutes at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... and then plated in a culture plate pre-coated with 10 µg/mL anti-CD3Ɛ antibody (Biolegend). Culture medium was exchanged to R10 containing IL-2 and the cell density was adjusted to be 0.5 – 1x106 cells/ml every 24 h.
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bioRxiv - Cancer Biology 2022Quote: ... Cell death was assessed by flow cytometry using 0.1μg/mL propidium iodide (PI, Biolegend, cat no: 421301) and neutral beads (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: ... purified anti-SIRPα mAbs (clone SE5A5) and G1 isotype mAbs were used at 10 µg/mL (BioLegend). Anti-Mac-1 was reconstituted by mixing anti-CD11b (clone ICRF44 ...
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bioRxiv - Cancer Biology 2022Quote: ... Approximately 1×106 cells were aliquoted in 5 ml tubes and incubated with Fc Blocking antibody (Biolegend). Antibodies used for immune profiling are detailed in Supplementary Table 1 ...
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bioRxiv - Immunology 2020Quote: ... injected with 200 μL biotin-conjugated anti-CD45 mAb in PBS (15 μg/mL, Biolegend, clone 104) into the lateral tail vein ...