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Citations for BioLegend :
3451 - 3500 of 3701 citations for Marker 7 Pcb Mixture W Pcb 118 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Final volumes were adjusted to a total of 2.5 mL per reaction and placed into a MojoSort magnet (480019, BioLegend) for 5 min ...
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bioRxiv - Immunology 2022Quote: ... was counted in an automated Vicell cell counter before 2X106 cells/well were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM) supplemented with 10% FBS and 10ng/ml Macrophage colony- stimulating factor (M-CSF) (Biolegend), (growth media ...
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bioRxiv - Microbiology 2023Quote: ... MBL) or H-2q-restricted LCMV NP118 peptide (MBL) and 50 U/mL of recombinant mouse IL-2 (BioLegend) for 1 h ...
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bioRxiv - Immunology 2023Quote: ... 130-120-662) for 3 days in 24-well plates coated with 3μg/ml anti-CD3 (145-2c11; Biolegend) and 3μg/ml anti-CD28 (37.51 ...
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bioRxiv - Cancer Biology 2023Quote: ... naïve CD4+ T cells were co-cultured with APCs at indicated ratio in the presence of 0.5 μg/mL (anti-CD3 BioLegend), 0.5ng/mL TGFβ (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... expanded CD4+ T cells were cocultured at different ratios with CFSE/CTV labeled CD8+ T cells and stimulated with Mouse T-Activator CD3/CD28 Dynabeads at 2 cells per bead ratio and 0.5ng/mL IL-2 (BioLegend). Cell culture media consisted with RPMI 1640 ...
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bioRxiv - Immunology 2023Quote: ... They were cultured in Th1-polarising media consisting of R10 with 1 μg/mL anti-mouse CD28 (102101, BioLegend), 5 μg/mL anti-mouse IL-4 (504102 ...
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bioRxiv - Immunology 2023Quote: ... the cells were harvested and washed twice before subjecting to propidium iodide (PI) solution (10 µg/ml) (BioLegend, #421302) for 10 min ...
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bioRxiv - Bioengineering 2023Quote: ... The 5x diluted streptavidin-coated nanovial suspension was then incubated with an equal volume of 71.5 μg/mL of biotinylated anti-human VEGF antibody (Biolegend) for 30 minutes at room temperature on a tube rotator and washed ...
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bioRxiv - Bioengineering 2023Quote: ... Excess recombinant VEGF-A was washed before reconstituting as 5x dilution of nanovials and incubating with equal volume of 71.5 μg/mL AF647 anti-VEGF-A (Biolegend) at room temperature for 30 minutes in the dark on a tube rotator ...
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bioRxiv - Bioengineering 2023Quote: ... This suspension was incubated with an equal volume of 71.5 μg/mL of oligo-barcoded anti-VEGF-A detection antibody (Biolegend) for 30 minutes at 37°C with gentle vortexing every 10 minutes before washing excess antibody with one high-dilution wash (>100x nanovial volume) ...
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bioRxiv - Bioengineering 2023Quote: ... and stained with Annexin V-FITC (5uL in 0.14 mL Annexin V Binding Buffer, #640914; Biolegend, San Diego, CA) for 10 minutes at 4°C in darkness ...
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bioRxiv - Immunology 2023Quote: ... a 10X molar excess of biotinylated RBD-Avi protein was incubated with 0.5 mg/ml of streptavidin-AlexaFluor 488 or streptavidin-AlexaFluor 647 (BioLegend) for 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... were coated with 1 μg/mL capture antibodies (IFNγ: clone R4-6A2, BD Pharmingen; or IL-17A: clone TC11-18H10.1, Biolegend) diluted in carbonate buffer ...
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bioRxiv - Immunology 2023Quote: ... 2×105 purified total B wells from B-Tfam mice placed in a 6mm transwell chamber with 5μm pore size and incubated for five hours in the presence or absence of murine CXCL12 (200ng/ml, Biolegend) with or without mitoTempo (100μM ...
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bioRxiv - Immunology 2023Quote: ... A 96 well plate was pre-incubated with a 5 µg/ml solution of anti-mouse CD3e (100314, Biolegend) in sterile PBS at 37°C for 2 h ...
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bioRxiv - Physiology 2023Quote: ... 120 µL was diluted in in 1x MojoSort buffer (2.5 mL) and placed in a MojoSort Magnet (Cat# 480019/480020, BioLegend) for 5 min for magnetic separation ...
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bioRxiv - Immunology 2023Quote: Activation consisted of 10μg/mL plate-bound anti-human CD3 antibody (300437) and 1μg/mL soluble anti-human CD28 antibody (302902) purchased from BioLegend, USA ...
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bioRxiv - Immunology 2023Quote: ... Activation consisted of 5μg/mL plate-bound anti-mouse CD3ε antibody (100340) and 5μg/mL soluble anti-mouse CD28 antibody (102116) purchased from BioLegend, USA ...
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bioRxiv - Immunology 2023Quote: ... Enriched CellTrace Violet-labeled OT-I CD8+ T cells were resuspended at 20,000 cells/100uL in complete RPMI + 10ng/mL of recombinant mouse IL-2 (Biolegend). As positive control ...
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bioRxiv - Biochemistry 2023Quote: ... Naïve CD4+ T cells were then activated with anti-CD3 (Biolegend, Cat#100340, 1:500 dilution to 5μg/mL), anti-CD28 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... were given by tail-vein intravenous (i.v.) injection in 200 μL of PBS with 1 μg/mL of anti-CD3 antibody (OKT3, Biolegend). Monthly post HPC transplant ...
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bioRxiv - Immunology 2023Quote: ... 3x105 sorted naïve CD4 T cells were stimulated with plate-bound anti-CD3 (145-2C11, Cytek) (5 μg/ml) and soluble anti-CD28 (clone 37.51, Biolegend) (1 μg/ml ...
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bioRxiv - Immunology 2024Quote: ... lymph node suspension from 2 mice per biological replicate were activated with 0.5 µg/mL anti-mouse CD3 (Biolegend) and 0.5 µg/mL anti-mouse CD28 (eBioscience ...
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bioRxiv - Immunology 2024Quote: ... HVEM binding to BTLA was assessed by incubating cells with the HVEM-Fc recombinant protein (20 µg/ml; Biolegend) followed by a staining with an Alexa488 conjugated-anti-IgG1 secondary Ab (clone HP6069 ...
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bioRxiv - Immunology 2024Quote: ... resuspended in 1 ml of PBS containing 1 µl live/dead stain (Zombie UV Fixable Viability kit (Biolegend, # 423107)) and incubated on ice for 30 min ...
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bioRxiv - Immunology 2024Quote: ... in presence (stimulated condition) or absence (unstimulated condition) of 5 μg/ml anti-human CD3ε (clone OKT3; #317302, BioLegend) in PBS at 37°C for 2h ...
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bioRxiv - Immunology 2024Quote: ... cells were resuspended in 50 µL Fc blocking medium (2.5 µg/ml purified anti-mouse CD16/32 Antibody (BioLegend) in Cell Staining Buffer (BioLegend)) ...
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bioRxiv - Cancer Biology 2021Quote: ... NKG2D ligands were blocked on the cancer cell lines and single cells of tumor organoids by incubating the target cells with 12 µg/mL anti-MICA/B (clone 6D4, BioLegend), 1 µg/mL anti-ULBP1 (clone 170818 ...
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bioRxiv - Cancer Biology 2021Quote: 96-well round-bottom plates were coated with either 2.5 µg/mL anti-CD3 (Biolegend, clone 145-2C11,Cat# 100359) or HIgG (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: ... Unlabeled cells were then centrifuged at 300 x g and labeled with a cocktail of 1 μg/ml of PerCP-Cy5.5 anti-EPCAM antibody and Zombie Aqua viability stain (Biolegend 423101) diluted 1:400 from stock concentration in FACS buffer.
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bioRxiv - Cell Biology 2020Quote: ... followed by incubation on ice with labeling cocktail consisting of 1 μg/ml of PerCP-Cy5.5 anti-EPCAM antibody and Zombie Aqua viability stain (Biolegend 423101) diluted 1:400 from stock concentration in FACS buffer ...
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bioRxiv - Immunology 2021Quote: ... total thymocytes were processed to single cell suspension in 10% FBS/HBSS (20×106 cells/ml) and incubated with rat anti-mouse CD24 antibodies (M1/69, Biolegend) (30 μg/100 ×106 cells ...
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bioRxiv - Cell Biology 2022Quote: ... 105 - 106 cells were resuspended in FACS buffer with 1 µg/ml TruStain FcXTM anti-mouse CD16/32 antibody (BioLegend) to block for 5 minutes before adding conjugated surface antibodies in FACS buffer at a 2X concentration ...
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bioRxiv - Immunology 2020Quote: ... splenocytes from OT-II/Rag1/CD45.1 mice were cultured in a-CD28 coated plates (Biolegend, Cat. No. 102115, 1ug/ml) with complete RPMI (10%FBS ...
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bioRxiv - Immunology 2021Quote: ... Nunc ELISA plates were coated with 1 μg/ml Spike S1+S2ECD-His recombinant protein (SinoBiologicals) in ELISA coating buffer (Biolegend) overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... were produced by differentiating peripheral blood mononuclear cells (PBMC) in RPMI 1640 medium containing human M-CSF (10ng/mL, Biolegend) and 10% human platelets-free plasma for 5-7 days (42 ...
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bioRxiv - Immunology 2020Quote: ... The cells were activated in 96-well round bottom plates that were coated with 3ul/ml anti-CD3e (BioLegend #100202) and 5ul/ml anti-CD28 (BioLegend #102102 ...
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bioRxiv - Immunology 2021Quote: ... 0.05 mM β-mercaptoethanol) on a 96-well flat bottom plate coated with anti-CD3 antibody (10µg/ml, cat. # 317325, BioLegend). 7 x 103 DC3 or 5 x 104 monocytes were used per well ...
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bioRxiv - Cell Biology 2022Quote: ... lung cells were brought to a single-cell suspension via collagenase incubation and then stained for flow cytometry at a concentration of 5×106 cells/mL with Cd31 (Biolegend) and 3μM ALOD4-47.
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bioRxiv - Microbiology 2022Quote: ... the supernatant was discarded and the pellet was resuspended in 10 ml of cold 1X Red Blood Cell Lysis buffer (Biolegend). Samples were vortexed and incubated in the dark for 10 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... The plates were washed twice with PBS plus 2 % FBS and subsequently coated with 10 μg/ml unconjugated anti-CD16 (3GB, BioLegend) in PBS overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Anti-Mac-1 was reconstituted by mixing anti-CD11b (clone ICRF44) and CD18 (clone TS1/18) mAbs at a final concentration of 10 µg/mL for each (BioLegend). Anti-CD3 mAbs (clone UCHT1 ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were then washed once with ice-cold FACS buffer and incubated with 2 μg/mL PE anti-human IgG Fc (BioLegend) at 4 °C with gentle shaking in the dark for 1 hour ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were washed once with ice-cold FACS buffer and incubated with 1 μg/mL PE anti-human IgG Fc (Biolegend) at 4 °C with gentle shaking in the dark for 1 hour ...
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bioRxiv - Bioengineering 2022Quote: ... Streptavidin-coated nanovials were reconstituted at a five time dilution in Washing Buffer containing 140 nM (20 μg/mL) of each biotinylated antibody or cocktail of antibodies: anti-CD45 (Biolegend) and anti-IFN-γ (R&D Systems) ...
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bioRxiv - Biochemistry 2022Quote: ... neutrophils (1×106 cells/mL) were added to each well in the presence of APC conjugated anti-CD11b antibody CBRM1/5 (BioLegend) at 1 μg/mL ...
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bioRxiv - Microbiology 2021Quote: ... cell surface markers were detected by a 30 minute-incubation at 4°C with 4 µg/mL Pacific Blue-conjugated mouse anti-CD14 (clone 63D3, Biolegend), a 1:20 dilution of APC-conjugated mouse anti-CD163 (clone REA812 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 U/ml erythropoietin) for 1 hour following disaggregation before being stained (anti-CD71 Brilliant Violet 421, 1:5000 dilution – 113813 Biolegend, RRID ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were activated for 24h using 2 μg/ml of anti-Cd28 and anti-Cd3ε antibodies (102116 and 100340, BioLegend).