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Citations for BioLegend :
3201 - 3250 of 3701 citations for Marker 7 Pcb Mixture W Pcb 118 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: Plates for CD8+ T-cell activation were coated with 5μg/mL α-CD3 (Biolegend, 100239) in phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2024Quote: ... further supplemented with 20% heat-inactivated foetal bovine serum and 40ng/mL M-CSF (Biolegend). Half of the cell suspension was further supplemented with 20μg/mL vancomycin hydrochloride (Fisher BioReagents) ...
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bioRxiv - Immunology 2024Quote: ... the cells were incubated with 1ug/ml Ultra-LEAFTM purified anti-CD28 (clone CD28.2, Biolegend) agonistic antibody ...
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bioRxiv - Cell Biology 2024Quote: ... human donor RGCs were pretreated with 5 mg/mL annexin V (BioLegend, San Diego, CA), a calcium-dependent membrane-bound protein with high affinity to PS ...
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bioRxiv - Immunology 2024Quote: ... then floated on 2 μg/ml anti-Vγ5 and anti-CCR6 (BioLegend, San Diego, CA) for 1 hour at 37°C with 5.0% CO2 ...
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bioRxiv - Biochemistry 2024Quote: ... were coated overnight with purified anti-IL2 antibody (clone JES6-1A12, Biolegend, 2 μg/mL) diluted in PBS (50 μL/well) ...
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bioRxiv - Immunology 2024Quote: ... 10 µg/ml of Ultra-LEAFTM purified anti-mouse TNF antibody (clone MP6XT22, Biolegend, #100340), 10 µg/ml of Ultra-LEAFTM purified anti-mouse IL-17A antibody (clone QA20A33 ...
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bioRxiv - Neuroscience 2022Quote: ... Splenocytes were then filtered through a 40μM filter and stained with an antibody cocktail [Pacific Blue anti-CD45.1 (Biolegend no. 110722; 1:100 dilution), APC anti-CD45.2 (Biolegend no ...
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bioRxiv - Developmental Biology 2022Quote: Single-cell suspensions were obtained as described above and subsequently blocked for 5-minutes on ice with Fc block CD16/32 (Biolegend, #101302, 1:100), followed by the addition of antibodies for 30-minutes on ice ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were washed and resuspended in 100 μl cold differentiation media with 2μl anti-MHCI (Biolegend, anti-HLA-ABC, clone W6/32, 311406). After a 30min incubation at 4□C ...
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bioRxiv - Pathology 2019Quote: ... The ears were fixed in 4% paraformaldehyde for 20 minutes at 4°C and incubated overnight in 0.05% Triton X-100 in PBS containing anti-human CD31 (Biolegend, San Diego, CA, USA) antibody conjugated to either PE or AF647 in a 1:200 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed with 2 ml of FACS buffer followed by 300 μl of FACS buffer and resuspended in a total of 100 μl of Brilliant Violet 650 mouse anti-human CD45 (Biolegend catalog no. 304043), Alexa Fluor 700 mouse anti-human CD4 (Biolegend ...
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bioRxiv - Neuroscience 2020Quote: ... anti-neurofilament to a phosphorylated heavy chain neurofilament (NF-H; mouse anti-human monoclonal antibody SMI-34, dilution 1:100, epitope retrieval time 10 min, Biolegend 835503; Biolegend, San Diego, CA). Hemosiderin deposition was evaluated on a section with only counterstain ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with FACS buffer followed by PBS wash and stained with 100 μl of 1:1000 dilution of fixable Zombie Aqua (BioLegend, San Diego, CA) dye for 20 minutes at 4°C in dark ...
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bioRxiv - Immunology 2020Quote: ... and then incubated in the dark for 30 min at 4°C: Myeloid-lymphocyte common lineage panel – TruStain FcX (anti-CD16/32) (clone: 93; dilution: 1:100, BioLegend, San Diego, CA), CD45-Pacific Blue (clone ...
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bioRxiv - Immunology 2021Quote: ... followed by a single wash and a secondary staining with anti-mFc IgG1: PE and/or APC [clone RMG1-1] at 1:100 (Biolegend, Cat: 406608/406610). After enrichment for S1 ...
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bioRxiv - Cancer Biology 2022Quote: ... a sequential gating strategy was used to analyze cancer cells by staining with the following FACS antibodies (Figure S3F): CD45-Pacific Blue (BioLegend 103126, 1:100), CD31-Pacific Blue (BioLegend 102422 ...
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bioRxiv - Immunology 2023Quote: ... 500 uL cell suspension was added to 12x75mm polystyrene tubes and incubated with 100 uL TruStain FcX™ (BioLegend, San Diego, CA, USA) on ice for 20 min ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were suspended at 3×106 cell/ml and incubated for 30 min at 4C with 1:100 dilutions of the following antibodies: [APC dump channel: Ter-119 (Biolegend cat no. 116212), CD45 (Biolegend cat no ...
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bioRxiv - Immunology 2022Quote: ... Cells were centrifuged for 5 min at 500 x g and 4 °C and stained with Fc receptor blocker (TruStain fcX™, BioLegend, 1:100) and viability stain (Zombie Aqua™ Fixable Viability Kit ...
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bioRxiv - Immunology 2023Quote: ... All cytokine antibodies were used at 1:100 dilution in 1x BD Perm/Wash buffer (anti-IFN-γ (APC/Cyanine7 or PE, Biolegend, #505849, #505808) and anti-TNF-ɑ (BV711 Biolegend ...
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bioRxiv - Neuroscience 2024Quote: ... singlesuspension cells were stained at the cell surface in different panels with PerCP5.5-conjugated anti-CD45 (1:100, BioLegend Cat# 103132 (also 103131), RRID:AB_893340) ...
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bioRxiv - Cell Biology 2024Quote: ... non-specific Fc receptor binding was blocked using 1:100 dilution of TruStain FcX anti-mouse CD16/32 antibody (BioLegend, RRID:AB_1574975, Cat#101320). 5 min after Fc blocker treatment ...
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bioRxiv - Physiology 2024Quote: ... collected into FACS tubes (Falcon, cat no. 352052) and incubated with the following antibodies: AF700-labeled CD54 (1:100, BioLegend, cat no. 353126) and PE/Cy7-labeled CD62E (1:100 ...
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bioRxiv - Genomics 2020Quote: ... and the cell pellet was resuspended to 108 cells/mL in 1X Nanobead buffer (BioLegend #480017). Contaminating immune cells were depleted using anti-CD45 Magnetic Nanobeads (BioLegend #488028 ...
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bioRxiv - Immunology 2019Quote: ... and acquired as such or after labelling with either 2 μg/ml anti-CD14-APC (61D3; Biolegend), 2 μg/ml anti-CD41-PE (HIP8 ...
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bioRxiv - Immunology 2021Quote: ... the cells were lysed and fixed with 2 ml of 1x RBC Lysis/Fixation Solution (Biolegend) for 15 minutes at RT and washed with cold FACS-buffer (PBS with 1 % FCS) ...
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bioRxiv - Immunology 2020Quote: ... Samples were normalized to 0.5 mg/mL protein in Legendplex assay buffer (Biolegend, San Diego, CA) using a Bradford Assay (BioRad ...
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bioRxiv - Immunology 2022Quote: ... recombinant human IL-12p70 and recombinant human IL-18 at 10ng/ml (Biolegend; San Diego, CA).
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bioRxiv - Immunology 2021Quote: ... Co-stimulation was added with peptides: 1μg/mL anti-CD49d (Clone 9F10, BioLegend Cat# 304301, RRID:AB_314427) and CD28-ECD (Clone CD28.2 ...
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bioRxiv - Immunology 2022Quote: ... Two washed in Fly-P buffer followed by incubation in 10ug/ml streptavidin-phycoerythrin (PE, Biolegend) for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... A second staining with 10 μg/ml IgG2b mouse monoclonal antibody 9C4 directed against EpCam (Biolegend) allowed the identification of epithelial cells by indirect detection with anti-IgG2b Cyanine-3 labelled secondary Ab (Jackson ImmunoScience ...
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bioRxiv - Cell Biology 2020Quote: ... treated cells were harvested and stained with 5 μg/ml propidium iodide and Annexin V (Biolegend) in Annexin V-binding buffer (10 mM Hepes pH 7.4 ...
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bioRxiv - Immunology 2020Quote: ... Th2 cells were cultured for 4 days with plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-4 (4 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... Th17 cells were differentiated in the presence of plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-6 (20 ng/ml ...
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Blunted Fas signaling favors RIPK1-driven neutrophil necroptosis in critically ill COVID-19 patientsbioRxiv - Immunology 2021Quote: ... Ultra-LEAF™ purified anti-human CD95 (FAS) blocking antibody (10 μg/ml, clone A16086F, Biolegend) or respective DMSO and H2O control were added prior to incubation ...
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bioRxiv - Immunology 2020Quote: ... after which time cells were stained with 5 μg/ml CD8-APC (clone SK1; Biolegend, UK), harvested and CSFE dilution measured by flow cytometry ...
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bioRxiv - Immunology 2019Quote: ... at 0.1μg/ml each in 100μl total volume: AF647-conjugated anti-human/mouse CD11b (#101220, BioLegend), PE-conjugated anti-mouse Ly6C (#101220 ...
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bioRxiv - Immunology 2020Quote: ... wells were supplemented with 10 μg/ml of αLFA1 antibody (Clone: TS 1/18, BioLegend, 302116) or with 10 μg/ml of αFAS-L antibody (BD ...
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bioRxiv - Systems Biology 2021Quote: ... centrifuged and resuspended in 5 ml of 1X red blood cell (RBC) lysis buffer (420301, BioLegend) for 5 min on ice ...
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bioRxiv - Immunology 2021Quote: 96-well U-bottomed plates were coated with 100μL of 0.5μg/mL streptavidin (BioLegend Cat#270302) at room temperature (18-25°C ...
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bioRxiv - Genetics 2020Quote: ... Cells were stimulated for 3 hours with 2.5 μg/ml of anti-CD3 (Biolegend, catalog 317315) and anti-CD28 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... with or without the inclusion of interleukin-4 at 20ng/mL (wt/vol, IL-4, BioLegend), interferon-g at 2.5ng/mL (wt/vol ...
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bioRxiv - Immunology 2022Quote: ... Jurkat cells were labelled with 25 μg/ml anti-human CD3 antibody (BioLegend, 317304, clone OKT3) and 3 μg/ml recombinant annexin V (BioLegend ...
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bioRxiv - Neuroscience 2022Quote: ... + 1% Glutamax + 1% Pen-strep + 0.1 ug/ml IL-34 (PeproTech, #200-34; Biolegend Inc, #577904) + 10 ng/ml GM-CSF (PeproTech ...
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bioRxiv - Immunology 2022Quote: ... in B cell medium in the presence or absence of 10 μg/ml anti-CD86 (BioLegend) or 1 μg/ml CD40L (Peprotech ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were incubated with 1 μg/mL of phycoerythrin (PE)-conjugated anti-human IgG Fc (BioLegend). Cells were washed once and resuspended in ice-cold FACS buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... and pre-incubated with anti-mouse Fc block at 10 μg mL-1 (#101320, BioLegend, USA) or anti-human Fc block (#422302 ...
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bioRxiv - Cancer Biology 2022Quote: ... we incubated the beads with 3.5 μg/ml biotinylated anti-CD81 antibody (BioLegend, clone TAPA-1) at the room temperature for 40 min ...
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bioRxiv - Immunology 2022Quote: ... coated flat bottomed 96 well plates with soluble anti-CD28 (2 μg/mL) (ULTRA-LEAF Biolegend), and with or without human recombinant soluble BAFF at 500 ng/mL ...