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Citations for BioLegend :
3151 - 3200 of 7138 citations for Primary Human Melanocyte Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... cells were washed with Cell Staining Buffer and resuspended in Fixation Buffer (BioLegend) for 15 minutes ...
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bioRxiv - Immunology 2024Quote: ... cells were suspended in complete RPMI media containing cell activation cocktail (Biolegend, 423303) and subsequently incubated at 37°C in a CO2 incubator for 4 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... Obtained single cells were then activated using a cell activation cocktail (423301, Biolegend) and cultured for 6 h at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... ATCC MM.1S cells were washed three times with cell staining buffer (BioLegend) and stained with 5 μM MitoSOXTM Red (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were passaged with Accutase cell detachment solution (BioLegend, San Diego, CA, USA) and seeded on new dishes ...
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bioRxiv - Immunology 2023Quote: Cells were analyzed for cell surface markers using fluorophore-conjugated antibodies (BioLegend, eBioscience). Cell surface staining was performed in FACS Buffer (2% FBS and 2 mM EDTA in PBS ...
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bioRxiv - Physiology 2023Quote: ... cells resuspended in Cell Staining Buffer were stained with Zombie NIR (BioLegend, #423105) for 10 min at 25°C ...
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bioRxiv - Immunology 2023Quote: ... Pooled 8:2 CD45+:CD45-cells were resuspended in Cell Staining Buffer (BioLegend) and stained with a pool of 137 TotalSeq-C antibodies (Table ...
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bioRxiv - Genomics 2023Quote: ... cells were labeled with ten different antibodies using the cell hashing protocol (BioLegend)131 and loaded into 16 lanes ...
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bioRxiv - Immunology 2024Quote: ... followed by CD16a.NK-92 cells (5 × 104 cells/well) with brefeldin A (Biolegend), Golgi Stop (BD Biosciences ...
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bioRxiv - Biochemistry 2020Quote: ... the cultured cells were harvested and incubated with either fluorescein isothiocyanate (FITC)-conjugated anti-human FR-β antibody (m90916, a gift from Philip S. Low) or allophycocyanin (APC)-conjugated anti-human FR-β antibody (mouse IgG2a, BioLegend, San Diego, CA, USA), and isotype controls (mouse IgG-FITC ...
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bioRxiv - Genomics 2019Quote: ... cells were washed once in PBS and once in Flow buffer (PBS with 2% BSA) and stained with anti-human CD47-APC antibody (clone CC2C6, BioLegend, Cat. #323123/4, RRID: AB_2716202/3) in Flow buffer (20µl antibody/40×106 cells/ml ...
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bioRxiv - Immunology 2023Quote: CD8+ T cells were isolated from in vitro treated CAR-T cells using Mojosort™ Mouse CD8 T Cell Isolation Kit (Biolegend, 480035). After washing cells with PBS twice ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were stimulated as a single-cell suspension at a concentration of 4-5 x 106 cells/mL with the Cell Activation Cocktail (Biolegend, Cat # 423301) and incubated at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2019Quote: ... Red blood cells were osmotically lysed and CD4+ T-cells were isolated using a MojoSort Mouse CD4+ T Cell Isolation Kit (Biolegend, San Diego, CA). Purified CD4s were labeled with Cell Proliferation Dye eFluor450 (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated with Fc-blocking anti-CD16/32 anti-bodies followed by cell hashing antibody (1 μg/ml, BioLegend Cell Hashing A) (Stoeckius et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cell RNAseq data for the 7 PDA cell lines were generated as following: Cell lines were trypsinized and resuspended in Cell Staining Buffer (Biolegend, Cat. No. 420201) and incubated with proper Total Seq B antibodies using recommended protocol from Biolegend ...
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bioRxiv - Immunology 2024Quote: ... viable T cells were enriched using the MojoSort Mouse CD3 T Cell Isolation Kit and Mouse Dead Cell Removal Kit (BioLegend, San Diego, CA).
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bioRxiv - Immunology 2023Quote: CD3+ T cells were purified from BALB/c mouse spleen cells with the MojoSort mouse CD3 T cell isolation kit (Biolegend, San Diego, CA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Dead cells were labelled with commercially available Zombie UV Dead Cell Staining Kit (BioLegend). For the analysis of intracellular cytokines ...
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bioRxiv - Immunology 2021Quote: ... and red blood cells were lysed with 1x red blood cell lysis buffer (BioLegend). For organ harvest ...
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bioRxiv - Developmental Biology 2022Quote: ... Red blood cells were eliminated from dissociated cells using an RBC lysis buffer (BioLegend). Cells were then stained with Aqua live/dead viability dye (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated with dead cell dye Zombie red (1:1000 in PBS, Biolegend) for 15 min ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then washed with 2 ml of cell staining buffer (Cat. #420201, BioLegend), resuspended in 300 μl PBS and measured using an LSR II flow cytometer (BD Biosciences) ...
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bioRxiv - Molecular Biology 2021Quote: ... Collected cells were re-suspended in 100 µL Red Cell Lysis Buffer (BioLegend, USA) and incubated for 5 min ...
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bioRxiv - Cell Biology 2019Quote: ... anti-CD3-APC for T-cells and anti-CD19-APC for B-cells (BioLegend).
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bioRxiv - Cancer Biology 2022Quote: ... Red blood cells (RBC) were removed from cell suspension using RBC lysis buffer (BioLegend). The dissociated cells were washed with PBS and resuspended in 1% FBS in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Red blood cells (RBCs) were lysed by incubating cells with RBC lysis buffer (BioLegend) for 15 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... PFA-fixed cells PBMCs were resuspended and blocked in Cell Staining buffer (BioLegend Inc.) containing Fc blocking antibody against CD16/CD32 (BioLegend Inc ...
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bioRxiv - Cancer Biology 2020Quote: ... Single cell suspension of spleen cells were stained for B220 (BioLegend; clone: RA3-6B2), CD19 (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then washed and stained with the Zombie NIR (cell death marker, Biolegend). The stained cells were analyzed by flow cytometry with a FACSCanto II cytometer (BD Biosciences) ...
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bioRxiv - Cell Biology 2020Quote: ... Cell pellets were resuspended in 5 ml of red blood cell lysis solution (BioLegend) on ice for 5 min followed by the addition of 20 mL of complete media ...
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bioRxiv - Neuroscience 2022Quote: ... and the cell pellet was incubated with red blood cell (RBC) lysis buffer (Biolegend) for 5 mins at room temperature followed by a wash with 1% Bovine serum albumin in Phosphate buffer saline (PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were first stained with a cell viability dye Zombie Green (BioLegend, Cat# 423111) for 20 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... and spleens collected for isolation of CD4+ T cells and dendritic cells (Stemcell, Biolegend) for in vitro experiments.
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bioRxiv - Genomics 2024Quote: ... The cell pellets were then washed with 500μL of cell staining buffer (BioLegend 420201) and resuspended in either 100μL of 4% PFA or 250μL of Cytofix/Cytoperm fixation and permeabilization buffer (BD Biosciences 554714) ...
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bioRxiv - Immunology 2024Quote: CD4+ T cells were isolated with MojoSortTM Mouse CD4 T cell isolation kit (Biolegend) from spleens of WT mice with > 90% purity ...
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Convergence, plasticity, and tissue residence of regulatory T cell response via TCR repertoire prismbioRxiv - Immunology 2023Quote: ... Antibodies for magnetic cell isolation and fluorescence activated cell sorting were purchased from Biolegend, Thermofisher and TONBO BIO ...
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bioRxiv - Cell Biology 2023Quote: ... cells were depleted of red blood cells by using RBC Lysis Buffer (BioLegend, 420301), and 1 × 106 cells per sample were used for staining ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated using MojoSort Mouse CD4 T cell isolation kit (BioLegend). Upon isolation ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1-2 million cells were incubated in 45ul of Cell Staining Buffer (BioLegend) per million cells with Trustain FcX block (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... Red blood cells and dead cells were removed using an RBC lysis buffer (BioLegend) and a Dead Cell Removal kit (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: ... Stimulated cells were stained for cell surface expression of CD45-BV650 (Biolegend, clone HI30), CD3-FITC (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed off and antibody secreting cells were detected using IgG biotin (BioLegend) followed by streptavidin-alkalkine phosphatase (R&D Systems) ...
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bioRxiv - Immunology 2024Quote: ... Cells were re-activated ex vivo with Cell Activation Cocktail containing Brefeldin A (Biolegend) for 4h at 37°C.
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were pelleted and resuspended in 100 μl of Cell Staining Buffer (420201, BioLegend) followed by incubation with 1 μl of TruStain FcX™ PLUS (156603 ...
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bioRxiv - Immunology 2020Quote: ... B cells (Cat≠1115535; Biolegend), NK (Cat≠108905 ...
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bioRxiv - Immunology 2022Quote: ... or cell stim cocktail (Biolegend) for 18 hours at 37°C before supernatants where assessed for cytokine by ELISA (R&D systems).
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bioRxiv - Systems Biology 2019Quote: ... and CD326+ cells (Biolegend, 118212). After antibody staining ...
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bioRxiv - Genomics 2022Quote: ... in Cell Staining Buffer (BioLegend) for 10 minutes prior to the addition of 100 uL of the antibody cocktail ...