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Citations for BioLegend :
2951 - 3000 of 7138 citations for Primary Human Melanocyte Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... cells were incubated overnight at 4°C with purified monoclonal mouse anti-human ASC antibodies (TMS-1) (clone HASC-71) (Biolegend, California, USA) at a final concentration of 10 µg/ml diluted in 10% NGS + 0.5% Triton X-100 in PBS ...
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bioRxiv - Immunology 2021Quote: ... and bound antibodies were detected with a mouse IgG2a Alexa647-conjugated antibody directed against human IgG-Fc (BioLegend, San Diego, USA #409320). After washing the mean fluorescence intensities of transfected cells were determined with an Attune Nxt (Thermo Fisher Scientific ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... with 1 × 106 cells/mL 5000 rad irradiated PBMC + 50 U/mL IL-2 + 10 ng/mL IL-15 (both from NCI BRB Preclinical Biologics Repository) + 0.1 μg/mL each of anti-CD3 (Ultra-LEAF purified anti-human CD3 antibody clone OKT3; BioLegend, San Diego, CA) and anti-CD28 (Ultra-LEAF purified Anti-human CD28 antibody clone 28.2 ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were then evaluated for IFN-γ levels using the Legend Max ELISA kit with pre-coated plates (human IFN-γ) (Biolegend). Surface phenotype of cells that had been removed from the well was determined by flow cytometry using fluorochrome conjugated antibodies specific for 4-1BB or CD69.
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bioRxiv - Immunology 2022Quote: ... Cells were successively stained for 30 minutes on ice with the cocktail of fluorescently conjugated antibodies in 200 μl staining buffer (1X PBS + 2% FBS): 2 μg/ml biotinylated-RBD-Streptavidin-PE and FITC-anti-human IgG antibody (Biolegend, clone: M1310G05) and DAPI ...
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bioRxiv - Immunology 2019Quote: ... according to the manufacturer’s instructions and cultured in the above medium supplemented with 2 μg/mL anti-human RP105 antibody clone MHR73-11 (BioLegend Cat.# 312907).
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bioRxiv - Immunology 2020Quote: ... The cytokine production was measured using the ELISA Deluxe Set Human IL-8/CCL20/IL-1β (Biolegend, San Diego, California, United States), following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2021Quote: Enzyme-linked immunosorbent assays were completed using and according to the instructions found with the ELISA MAX™ Deluxe Set Human IL-2 (BioLegend #431804).
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bioRxiv - Immunology 2021Quote: ... P815 cells were used as target cells for human CTLs and pulsed for 1h at 37°C with 1µg/ml anti-CD3 antibody (clone UCHT1, Biolegend, Cat no. 300438). They were washed and resuspended at 1x105 cells/ml in killing assay media (RPMI without phenol red + 2% FCS ...
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bioRxiv - Immunology 2020Quote: ... Then these PBMCs was incubated with mixed antibodies cocktail at 4 °C for 30 min (the antibodies cocktail including FITC-anti-human CD19 antibody (Biolegend, clone: SJ25C1), BV421-anti-human IgD antibody (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated ON at 4°C with mouse anti-human CD66b antibody (BioLegend, 305102, 1:100 for both IHC and IF), anti-human CD8 (Invitrogen ...
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bioRxiv - Genetics 2021Quote: ... The cells were then incubated for 20 min at 4°C with the TotalSeq-C antibody mix: TotalSeq - C0048 anti-human CD45 Antibody (Biolegend; Cat# 368545), TotalSeq - C0103 anti-mouse/human CD45R/B220 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... HLA-A2 positive PBMC samples were further stained with PE labelled tetramer (home-made) and APC labelled human CD8+ antibody (BioLegend Cat#344721), and acquired with flow cytometer FACS Canto (BD).
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bioRxiv - Immunology 2020Quote: Cells were first incubated in 100 μL of PBS with 2mM EDTA for 15 min with 5μL Fc Block (Human TruStain FcX™, Biolegend, cat #422301) and a 1:500 dilution of Zombie UV viability dye (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... each sample was pelleted at 1,150 x g in a centrifuge for 5 min at room temperature and then stained with 50 µL of an antibody cocktail containing 1:50 diluted anti-human CD45-AF700 (Biolegend, clone HI30), 1:100 diluted anti-human CD3-FITC (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... each sample was pelleted at 1,150 x g in a centrifuge for 5 min at room temperature and then stained with 50 µL of an antibody cocktail containing 1:100 diluted anti-human CD3-FITC (BioLegend, clone UCHT1), 1:100-diluted anti-human CD4-PE (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed twice and resuspended in PBS containing 2% FBS before staining in the presence of Fc block (Miltenyi 130-059-901) with anti-human CD45 Alexa700 (clone HI30, BioLegend cat #304024) and anti-mouse CD45.1 PE-Cy7 (clone A20 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed 3 times with Cell Staining Buffer and stained with either Alexa Fluor (AF) 488 mouse anti-human BAX antibody (0.5 μg/mL, Biolegend, Cat. No. 633603) or AF488 Mouse IgG1 κ isotype control (5 μg/mL ...
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bioRxiv - Immunology 2024Quote: ... Primary human CD4+ T cells from CTLA4-deficient patients or healthy controls were purified from 5 million frozen PBMCs using a biotin labeled anti-human CD4 antibody (OKT4; BioLegend; 1:20) followed by magnetic separation with anti-biotin microbeads (Miltenyi Biotic ...
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bioRxiv - Neuroscience 2023Quote: ... and stained overnight with Alexa 488-conjugated Alexa Fluor® 488 anti-tubulin β3 and Alexa Fluor® 647 anti-mouse/human CD324 (E-cadherin) antibodies (#801203 and #147308, BioLegend). Each batch of anti-tubulin β3 antibody was titrated before use to minimize background staining ...
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bioRxiv - Microbiology 2023Quote: ... Nonspecific antigen binding to Fc receptors was inhibited via 10 minutes of blocking with Human TruStain FcX (BioLegend, San Diego, CA, USA). Cells were subsequently stained with mouse anti-human CD20 (Alexa-700) ...
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bioRxiv - Bioengineering 2023Quote: ... and stained with the following antibodies in FACS staining buffer (PBS with 2% Fetal Bovine Serum, 1% Penicillin-Streptomycin): anti-human CD31 (BioLegend, WM59, FITC), anti-human CD45 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... we employed the LEGENDplex™ Human Inflammation Panel 1 (13-plex) flow cytometry-based assay kit (#740809, BioLegend, San Diego, CA, USA). This panel allowed us for simultaneous quantification of 13 human inflammatory cytokines and chemokines (IL-1β ...
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bioRxiv - Immunology 2023Quote: ... Co-culture supernatant was collected following completion of culture and analyzed for IL-2 using the ELISA MAX™ Deluxe Set for human IL-2 (Biolegend, 431804) at a dilution of 1:5.
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bioRxiv - Biophysics 2023Quote: ... 100 μL of PRP was incubated with 1 μg/mL AF647-conjugated CD42b rabbit anti-human antibody (BioLegend, San Diego, CA, USA) to label the platelets ...
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in 90 μl of 1% BSA in PBS and Fc blocked with 10 μl Human Trustain FcX (Biolegend cat. 422302) for 10 minutes on ice then stained on ice for 45 minutes with a pool of 268 antibodies in 100 μl ...
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bioRxiv - Immunology 2023Quote: Freshly sorted CD8+ T lymphocytes of four healthy volunteers were labelled with anti-human Hashtag antibodies TotalSeq-C (Biolegend, San Diego, CA) and were pooled together ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 30 minutes at 4 °C using antigen probe (1.5 μL/well) and the following conjugated antibodies: APC anti-human CD3 (300412, BioLegend; 1 μL/well), PerCP/Cyanine 5.5 anti-human CD19 (302230 ...
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bioRxiv - Cancer Biology 2023Quote: ... the tumor organoids were treated with the immune checkpoint inhibitor Nivolumab (Selleckchem, Cat#A2002) and the Ultra-LEAF Purified Human IgG4 Isotype Control Recombinant Antibody (Biolegend, Cat#403701) at a concentration of 10 μg/mL ...
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bioRxiv - Immunology 2024Quote: ... then treated with Human TruStain FcX for blocking Fc receptors and stained with Brilliant Violet (BV) 421 anti-CD3 (BioLegend, clone OKT3), APC anti-human CD7 (BioLegend ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa Fluor® 700 anti-human CD41 antibody and Zombie NIR® fixable dye were obtained from BioLegend (San Diego, CA, USA). Anti-human CD62P conjugated PE antibody was obtained from Beckman Coulter (Brea ...
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bioRxiv - Microbiology 2024Quote: Suspensions of ∼106 HIV-infected TMCs in PBS with 1% FBS were incubated with BV711 anti-human CD3 antibody (Biolegend, catalog # 300464) at 4°C for 30 min ...
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bioRxiv - Bioengineering 2024Quote: Supernatants were collected after a 48-hour PBMCs and TCM incubation and immediately assessed for cytokine secretion using LEGENDplex Human Inflammation Panel 1 (Biolegend catalog #740809) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the PBMC staining panel for purity: anti-CD4 FITC (BioLegend catalog #317408, clone OKT4), anti-CD20 PerCP/Cy5.5 (BioLegend catalog #375510 ...
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bioRxiv - Developmental Biology 2022Quote: ... The cells were washed in Cell Staining Buffer (Biolegend) and stained with TotalSeq HTO antibodies for 30 min on ice ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were stained with Zombie Aqua cell dye (BioLegend) for 15 min at a 1:200 dilution in cell staining buffer (BioLegend) ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were washed with Cell Staining Buffer (Biolegend, #420201), then washed ...
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bioRxiv - Microbiology 2023Quote: ... lung cells were incubated with cell activation cocktail (Biolegend) and monensin for 4 h ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were stained with cell-hashing antibodies (TotalSeq, BioLegend) before cell capture ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were stained for dead cells (Zombie NIR; Biolegend) and surface markers (CD4 and CD8 ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were washed once with Cell Staining Buffer (Biolegend) prior to cell counting and antibody staining ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed three times with cell staining buffer and resuspended in Cell Staining Buffer (BioLegend) prior to flow cytometry using a MACSQuant Analyzer (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then incubated with primary antibody that recognizes an epitope within the humanized Aβ sequence using Purified Anti-B-Amyloid (BioLegend, Cat#803012, Lot#B251533) directly conjugated with horseradish peroxide (HRP ...
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bioRxiv - Immunology 2020Quote: ... ASC was stained using a primary antibody anti-ASC (polyclonal anti-PYD ASC, Santa Cruz or monoclonal anti-CARD ASC, Biolegend; 1:1000 dilution) or anti-Luciferase (Abcam ...
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bioRxiv - Cancer Biology 2019Quote: ... Sigma)for one hour at room temperature and incubated overnight at 4 °C with the following primary antibodies: anti-CD63 (BioLegend, San Diego, CA, USA), anti-Cytochrome c ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated for 1 hour at 37 °C with different primary antibodies: anti-β-III-tubulin (1:4000, 801202, Biolegend, San Diego, CA), anti-neurofilament SMI312 (1:1000 ...
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bioRxiv - Bioengineering 2021Quote: The Zombie Aqua™ Fixable Viability dye and all primary antibodies used for flow cytometry analysis were obtained from BioLegend (San Diego, CA, USA) (Table 1).
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bioRxiv - Cell Biology 2021Quote: ... followed by the addition of 1:20 (1:40 final) primary antibodies against LAMP1 (AF488- or BV605-conjugated; BioLegend cat. no. 328610 and 328634), and/or TFRC (FITC-conjugated ...
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bioRxiv - Genomics 2021Quote: ... 5% normal goat serum in PBS for 1 hour and incubated at 4°C overnight with primary antibody (Biolegend, cat. 613402, dilution 1:500), then at room temperature for 3 hours with secondary antibody (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2023Quote: ... Sections were then stained with primary antibody diluted in 1% BSA in PBS for 1 hour with rat anti CD68 (BioLegend 137002 1:400 dilution), rabbit anti Lyve1 (abcam ab14917 1:200 dilution) ...