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Citations for BioLegend :
3001 - 3050 of 7138 citations for Primary Human Melanocyte Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... Thereafter the sections were incubated overnight at 4°C with primary antibody (the primary antibodies for C3 and CFH were monoclonal from Santa Cruz raised in mouse and for NF-KB the monoclonal primary antibody was from Biolegend raised in mouse) diluted in 1X PBS followed by washing with 1X PBS and incubation for 60 minutes with biotinylated anti-mouse immunoglobulin ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.2 g/L NaN3] for 15 min after which the grids were incubated on a 20 μl drop of primary antibodies—1:10 dilution of 6E10 (Biolegend, San Diego, CA, USA) and anti-αSyn MJFR1 (Abcam ...
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used in this study: anti-tubulin beta-3 conjugated to Alexa Fluor 488 (BioLegend; AB_2562669; mouse IgG2a, 1:200), Alexa Fluor 555 (BD PharMingen ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant containing the dead cells was aspirated and cells were resuspended in Cell Staining Buffer (BioLegend).
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bioRxiv - Neuroscience 2022Quote: ... 1×106 cells from each iPS cell line were resuspended in 45 μL cell staining buffer (BioLegend) in a protein low-binding microcentrifuge tube separately and incubated with 5 μL Fc blocking reagent (BioLegend ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were harvested for cell cycle analysis using the Phase-Flow BrdU Cell Proliferation Kit (Biolegend, USA). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... Cell-hashing was performed by incubating 1X106 cells with Total-SeqB (B0301-B0304) cell-hashing antibodies (Biolegend) for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... and the cell pellet was treated to remove red cells (Red blood cell lysis buffer, 420301, Biolegend). After a wash ...
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bioRxiv - Biophysics 2022Quote: ... coverslips of each batch were incubated with 30 µg/ml anti-human IgG Fc (cross-reacts with rabbit) conjugated to Alexa Fluor-488 (Cat# 409321; BioLegend, San Diego, CA) for 45 minutes at room temperature in the dark ...
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bioRxiv - Cancer Biology 2019Quote: ... washed once with PBS at concentration 2×10^6/mL in RPMI1640 containing 10% FBS and 100U/ml of recombinant human IL-2 (BioLegend, San Diego, CA). After 72h ...
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bioRxiv - Bioengineering 2021Quote: ... Anti-FLAG epitope (DYKDDDDK, catalog# 637301) and horseradish peroxidase (HRP) Donkey anti-human IgG antibody (catalog #410902) were purchased from Biolegend (San Diego, CA). The secondary antibody anti-rat IgG HRP (catalog# 7077 ...
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bioRxiv - Neuroscience 2020Quote: 250 μg protein from each human brain sample lysate was used for immunoprecipitation (IP) with a combination of Tau12 (Biolegend, cat. no. 806501), Tau 5 (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants from triplicate wells of each condition were collected at 24 hrs and assayed for the IL-8 concentration using a human IL-8 enzyme-linked immunosorbent assay (ELISA) kit according to the instructions of the manufacturer (BioLegend, San Diego, CA). IL-8 values were reported as normalized values defined as a proportion increased or decreased compared to values obtained for J99 ...
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bioRxiv - Neuroscience 2020Quote: ... in PBS and then incubated with an Fc receptor blocking solution (Human TruStain FcX, 1:20 in staining buffer; Biolegend, San Diego, CA) for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... Aβ peptides were extracted from 250 µL human plasma using immunoprecipitation with anti-β-Amyloid 17-24 (4G8) and anti-β-Amyloid 1-16 antibodies (6E10, both Biolegend®) coupled to Dynabeads™ M-280 Sheep Anti-Mouse IgG magnetic beads (Thermofisher ...
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bioRxiv - Neuroscience 2022Quote: ... ELISA kit (CSB-E18033h, Generon) and monomeric α-Syn was analyzed using LEGEND MAX™ Human α-Synuclein ELISA Kit (SIG-38974, BioLegend). Then it was normalized by total protein per well using Pierce BCA Protein Assay Kit (23225 ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were stained for 20 minutes on ice with 1µg of BioLegend TotalSeq-A anti-human hashtag oligo antibody (BioLegend TotalSeq-A0251 to A0258). Cells were washed 3 times then pooled at equal ratios for sequencing ...
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bioRxiv - Biochemistry 2022Quote: Moles of product formed in hydrolysis reactions used for ACE2 activity and specificity profiling were determined by comparing area under the curve for LC chromatograms corresponding to these reactions to areas under the curve observed for control reactions in which > 95% input substrate conversion was achieved by incubation of substrate with 25 nM recombinant human ACE2 commercial standard (BioLegend, San Diego, CA) for between one and four hours pending peptide substrate ...
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bioRxiv - Pathology 2019Quote: ... IL-9 and IL-6 determinations (Human IL-4, IL-5, IL-9 and IL-6 MAX, Biolegend, San Diego, CA 92121 USA) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... freshly harvested PDX or EHS tumors were processed to single cells47 and stained with PE-conjugated anti-human CD298 PE (BioLegend; 1:50 dilution) and FITC-conjugated anti-mouse CD45 FITC (BioLegend ...
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bioRxiv - Pathology 2019Quote: ... The ears were fixed in 4% paraformaldehyde for 20 minutes at 4°C and incubated overnight in 0.05% Triton X-100 in PBS containing anti-human CD31 (Biolegend, San Diego, CA, USA) antibody conjugated to either PE or AF647 in a 1:200 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed with 2 ml of FACS buffer followed by 300 μl of FACS buffer and resuspended in a total of 100 μl of Brilliant Violet 650 mouse anti-human CD45 (Biolegend catalog no. 304043), Alexa Fluor 700 mouse anti-human CD4 (Biolegend ...
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bioRxiv - Neuroscience 2020Quote: ... anti-neurofilament to a phosphorylated heavy chain neurofilament (NF-H; mouse anti-human monoclonal antibody SMI-34, dilution 1:100, epitope retrieval time 10 min, Biolegend 835503; Biolegend, San Diego, CA). Hemosiderin deposition was evaluated on a section with only counterstain ...
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bioRxiv - Immunology 2021Quote: ... Bacteria were then resuspended in 0.2µm-filtered solutions of appropriate secondary reagents (Alexa 647-anti-human IgG, Jackson Immunoresearch 1:200, Brilliant Violet 421-anti-Rabbit IgG, Biolegend 1:200). This was incubated for 10-60 min before cells were washed as above and resuspended for acquisition on a BD LSRII or Beckman Coulter Cytoflex S ...
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bioRxiv - Immunology 2022Quote: Activated γδT cells were seeded in round-bottom plates with FITC conjugated anti-mouse CD107a (1D4B) antibody or anti-human CD107a (H4A3) (Biolegend, San Diego, CA). γδT cells were co-cultured with A20 or K562 at a ratio of 1:1 ...
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bioRxiv - Neuroscience 2022Quote: ... human Aβ was immunodepleted from TgCM with the mouse antibody 6E10 (Purified anti-β-Amyloid, 1-16 antibody BioLegend Cat# 803004, RRID: AB_2715854) and Protein G Sepharose beads (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... The panel of cytokines presented in Fig 5 was measured using the LEGENDplex™ Human Inflammation Panel 1 (13-plex) (BioLegend, Cat# 740809) kit ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were then stained overnight at 4 °C in a humidified chamber with mouse monoclonal anti-human CD68 (hCD68, 1:400, BioLegend, clone Y1/82A) and rabbit polyclonal anti-mouse IL-10 (mIL-10 ...
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bioRxiv - Bioengineering 2024Quote: ... cells were washed twice with 2% FACS buffer (1X PBS and 2% BSA) centrifuged and stained with human TruStain FcX (Biolegend, San Diego, CA) for 20 minutes ...
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bioRxiv - Bioengineering 2024Quote: The supernatant of CAR T and cancer co-culture systems was measured for their expression of cytokines using the LegendPlex Human Essential Immune Response Panel (Cat # 740930, BioLegend, San Diego, CA) (IL-4 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Determination of the cytokine levels in the supernatants was carried out using commercial ELISA kits for human IL-6 and TNF (BioLegend, San Diego, US). ELISA was carried out according to the manufacturer’s instructions.
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bioRxiv - Biophysics 2023Quote: ... Flow cytometry is further used to determine the expression profile of the integrins CD29 (PE anti-human CD29 antibody; Biolegend, cat. no. 303003) and CD18 (PE anti-human CD18 antibody ...
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bioRxiv - Microbiology 2023Quote: ... Samples were stained immediately after fixation with Alexa Fluor 647-labeled mouse-anti-human CD56 (10 μg/mL, Biolegend, San Diego, CA, USA) for 1 h and washed with PBS (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... coated glass bottom chamber and then labeled with 1ugꞏml−1 of Alexa Flour (AF) 488 conjugated anti-human CD45 antibodies (Biolegend, Cat# 304017, clone HI30) in 2% BSA in PBS for 20 min at RT ...
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bioRxiv - Immunology 2023Quote: ... prior to the end of the CD45- sort one vial of TotalSeq-C Human Universal Antibody Cocktail v1.0 (Biolegend, San Diego, CA, USA) was equilibrated to room temperature for 5min and then spun at 10,000g for 30 seconds ...
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bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the NK-92 staining panel: anti-CD107a (LAMP-1) FITC (BioLegend catalog #328606, clone H4A3), anti-CD56 (NCAM ...
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bioRxiv - Immunology 2024Quote: ... and activated for 72h with 10 µg/mL of plate-bound Ultra-LEAF™ purified anti-human CD3 monoclonal antibody (clone OKT3, BioLegend, cat. 317325) and 5 µg/mL of soluble Ultra-LEAF™ purified anti-human CD28 monoclonal antibody (clone 28.2 ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were fixed using a paraformaldehyde cell-fixation buffer (BioLegend). Flow cytometry was performed using a BD LSRFortessa Flow Cytometer ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were stained with Zombie Aqua cell viability dye (BioLegend) for 15 min at a 1:200 dilution in cell staining buffer (BioLegend ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were stained with Zombie Aqua cell viability dye (BioLegend) for 15 min at a 1:200 dilution in cell staining buffer (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... cells were processed with red blood cell lysis buffer (Biolegend) and plated in 1640 RPMI (Biological Industries ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed and resuspend in cell staining buffer (BioLegend). Flow cytometry data were acquired on a cytometer (LSR Fortessa TM ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed in the cell staining buffer (Biolegend, 420201). For experiments on cell surface antibody staining ...
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bioRxiv - Developmental Biology 2022Quote: ... Cell pellets were resuspended in Cell Staining Buffer (Biolegend, # 420201). Cell counting was performed using a Countess 3 Automated Cell Counter (ThermoFisher).
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bioRxiv - Genomics 2019Quote: ... cells were suspended in Cell Staining Buffer (BioLegend, Cat# 420201) and blocked using Human TruStain FcX reagent (BioLegend ...
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bioRxiv - Cell Biology 2021Quote: ... cell pellets were washed twice using cell staining buffer (BioLegend), permeabilized and treated with EdU detection solution ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell pellets were resuspended in cell staining buffer (Biolegend, 420201). In a round-bottom 96-well plate ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in 100 µl Cell Staining Buffer (Biolegend) added with 5 µl of Human TruStain FcX™ Fc Blocking reagent (Biolegend) ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were treated with red blood cell lysis buffer (BioLegend) for 2 minutes and washed with PBS ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and resuspend in cell staining buffer (BioLegend). Flow cytometry data were acquired on cytometer (LSR Fortessa TM ...