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Citations for BioLegend :
3051 - 3100 of 7138 citations for Primary Human Melanocyte Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were then washed twice in Cell Staining Buffer (BioLegend) and were sorted on a BD FACS Aria-II flow cytometer (BD Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were resuspended in 100μL of Cell Staining Buffer (Biolegend) and incubated in the dark for 15 minutes at room temperature following the addition of 5μL of Annexin V-APC antibody and 10μL of PI solution ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed in cell stain buffer (Biolegend, USA) and resuspended in 100uL of the same buffer ...
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bioRxiv - Immunology 2020Quote: Veri-cells (Biolegend) were reconstituted according to the manufacturer’s instructions in 2 ml of Tyto running buffer and then loaded into the sort cartridge ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then incubated with the following primary antibodies diluted in the incubation solution overnight: chicken anti-neurofilament NF-M (1:1000) (#822701, BioLegend, USA, previously #PCK-593P, Covance), rat anti-MBP (1:300 ...
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bioRxiv - Neuroscience 2020Quote: ... the neurons were fixed and stained as previously described38 using primary antibodies for β-3-Tubulin (TUJ1; 1:200; BioLegend, San Diego, CA, USA; 801201) and TH (1:300 ...
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bioRxiv - Genomics 2023Quote: ... cells were trypsinized and resuspended as a single cell suspension in Cell Staining buffer (BioLegend Cat. No. 420201) at a density of 6 × 105 cells in 100 µl ...
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bioRxiv - Microbiology 2021Quote: Concentrations of cytokines and chemokines present in the serum from SARS-CoV-2 infected AGMs were quantified using a multiplex bead-based assay (1:4 dilution)-the LEGENDPlex Non-Human Primate Cytokine/Chemokines 13-plex (BioLegend, San Diego, CA USA). Analytes detected by this panel are the following ...
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bioRxiv - Microbiology 2021Quote: ... Staining for human immunoglobulins was performed in 100 μL with 1:50 (v/v) of the detection antibodies: anti-human IgM Brilliant Violet 650 (clone: MHM-88, Biolegend® Cat. No. 314526), anti-human IgG PE/ Dazzle™ 594(clone ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed in FACS buffer and stained on ice for 30 minutes with an Alexa647-labelled anti-human IgG-Fc antibody (Biolegend, San Diego, USA, #409320). Alexa647 mean fluorescence intensities were determined for GFP-positive HEK-293T cells with a FACS Attune Nxt and analyzed with the software Flow Logic™ (IInivai Technologies Mentone ...
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bioRxiv - Immunology 2020Quote: ... Samples were incubated for 30 minutes in complete FACS buffer (PBS supplemented with 2% FBS) and blocked with Human TruStain FcX™ blocking reagent (BioLegend, San Diego, CA) prior to the staining with antibodies or BiTEs.
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bioRxiv - Immunology 2022Quote: Supernatants were assessed for cytokine production using the LEGENDplex™ Human Inflammation Panel 1 (13-plex) in a V-bottom plate per the manufacturer’s instructions (BioLegend, San Diego, CA, USA). Flow cytometry data were acquired on a BD Fortessa analyzer (BD Biosciences ...
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bioRxiv - Bioengineering 2024Quote: ... The sections were subsequently stained with a rat anti-human YAP antibody conjugated with PE (dilution: 1:500; clone W19260A; Biolegend, San Diego, CA, USA) for 2 hours at room temperature ...
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bioRxiv - Immunology 2024Quote: ... in the presence or absence of rIFNB (500 ng/mL human [Peprotech, Cranbury, NJ, US]; 50 ng/mL murine [BioLegend, San Diego, CA, US]), with or without neutralizing antibodies or EGTA ...
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bioRxiv - Immunology 2024Quote: ... Isolated CD4+ T-cells were either maintained in a naïve state by seeding in complete growth medium alone or underwent TH1-activation in tissue culture flasks pre-coated with anti-human CD3 antibody (10 µg/mL) (BioLegend, San Diego, CA, US) in complete medium supplemented with anti-human CD28 antibody (5 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 30 minutes at 4 °C using the following antibodies: Brilliant Violet 711™ anti-human CD4 (344648, BioLegend; 1 μL/well), APC anti-human CD3 (300412 ...
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bioRxiv - Immunology 2023Quote: ... TCR-transduced CD8+ T cells were rapidly expanded for 5 days using irradiated feeder PBMCs and medium containing 60U/ml IL-2 (Proleukin, Novartis) and Ultra-LEAF™ Purified anti-human CD3 Antibody (clone OKT3, BioLegend 317326, 1:2000). After 5 days ...
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bioRxiv - Bioengineering 2023Quote: ... wherein each well mixed 50 μL of X2 photobleached conjugated magnetic beads with 50 μL solutions at different concentrations of a recombinant human Interleukin-8 (IL-8) protein (#574202, BioLegend, San Diego, CA, USA). The overall well concentration levels were 0.05 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were stained for one hour using the following antibodies: Allophycocyanin (APC) anti-human CD274 (5 μL/test; BioLegend #329707; BioLegend, San Diego, CA, USA), APC anti-mouse CD274 (5 μL/test ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed 3 x with 1% BSA before incubating for 1 h in anti-human IgG secondary antibody labelled with either APC (BioLegend, #410712, dilution 1:100) or Phycoerythrin (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... flow cytometry staining master mix was prepared containing 1:200 dilutions of PE anti-mouse/human CD11b Antibody (BioLegend, Cat#:101208, clone M1/70), APC anti-mouse CX3CR1 (Antibody BioLegend ...
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bioRxiv - Immunology 2023Quote: ... pDCs were gated for flow cytometry with 1:20 dilution commercial mouse anti-human BDCA-2-Alexa488 (stock 200 ug/mL, BioLegend, San Diego, CA, USA) and 1:20 dilution mouse anti-human CD123 (IL-3)-PE (stock 200 ug/mL ...
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bioRxiv - Immunology 2024Quote: ... Blocking of CD47 was performed with purified mouse anti-human CD47 mAb (αCD47-fuFc) clone CC2C6 (cat. no. 323102, Biolegend, San Diego, CA, USA), and humanized anti-CD47 IgG2σ mAb (αCD47-siFc ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were washed twice with cold cell staining buffer (Biolegend, 420201) and resuspended in binding buffer ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were incubated in Red blood cell lysis buffer (420301, Biolegend) 3-4 min at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Red blood cells were lysed using red cell lysis buffer (Biolegend) and lymphocytes washed with ice-cold phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2022Quote: ... Cells were blocked with 3% BSA/PBS cell staining buffer (BioLegend) and incubated at 37°C for 2 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell suspensions were then washed with cell staining buffer (Biolegend 420201) prior to cell surface staining ...
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bioRxiv - Cell Biology 2022Quote: ... Cell pellet was resuspended in red blood cell lysis buffer (BioLegend) for 90 seconds and then diluted in 3x volume of PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... The cell suspension was washed using Cell staining buffer (Biolegend, #420201), then incubated with SYTOX™ Blue Dead Cell Stain (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... tumor single cell suspensions were stimulated with Cell Activation Cocktail (Biolegend) for 4 hours to allow accumulation of intracellular cytokines according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were washed twice with cold BioLegend’s Cell Staining Buffer (Biolegend), and then resuspended in Annexin V Binding Buffer at a concentration of 1.0 ×106 cells/ml ...
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bioRxiv - Genomics 2021Quote: ... cells were washed and resuspended with cell staining buffer (Biolegend, 420201) for hashing with TotalSeq-A anti-human Hashtag reagents (Supplementary Table 6 ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with red blood cell (RBC) lysis buffer (Biolegend) for 3 minutes at room temperature and were then washed in complete media once before analysis by flow cytometry.
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bioRxiv - Immunology 2022Quote: ... cells were stimulated with cell activation cocktail (Biolegend, San Diego, CA) and Brefeldin A for 4 hours prior to fixing and permeabilization ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed twice with cell staining buffer (BioLegend, Cat. # 420201) and transferred into a 5mL Polystyrene round-bottom tube with cell-strainer cap tubes (Falcon) ...
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bioRxiv - Physiology 2022Quote: ... Cells were then washed with Cell Staining Buffer (BioLegend 420-201) and incubated at 4°C for 30 minutes with secondary antibodies (Supplemental Table 5) ...
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bioRxiv - Cancer Biology 2024Quote: MM cells were washed three times with Cell Staining Buffer (BioLegend) and resuspended in their respective cell culture media with 100 nM MitoTracker Green (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... MM cells were washed three times with Cell Staining Buffer (BioLegend) and resuspended in tetramethylrhodamine ethyl ester (TMRE ...
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bioRxiv - Cancer Biology 2024Quote: MM cells were washed three times with Cell Staining Buffer (BioLegend) and fixed in 1x Fixation Buffer (4% paraformaldehyde ...
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bioRxiv - Cancer Biology 2024Quote: ... All cells were treated with Red Blood Cell Lysis buffer (Biolegend) and suspensions were filtered through 70 μm Nitex nylon mesh before further use.
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bioRxiv - Cell Biology 2023Quote: ... Cells were treated with 1:1000 diluted Cell Stimulation cocktail (Biolegend) overnight at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... Subsequent cell expansions were done using Accutase® Cell Solution (Biolegend) for gentle detachment ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were incubated in red blood cell lysis buffer (420301, Biolegend) 3-4 min at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Remaining cells were resuspended in cell staining buffer (BioLegend, CA, USA ) at a concentration of 2 × 105 – 1 × 106 cells per 100 µl ...
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bioRxiv - Cancer Biology 2023Quote: Single-cell suspensions from tumors or CD3 T cells isolated using MojoSort Mouse CD3 T Cell Isolation Kit (Biolegend) following the manufacture’s protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were gated on singlets and dead cells were excluded using a Zombie Green cell death dye (BioLegend, # 423111).
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed and incubated in primary conjugated antibodies (EZH2, BD Bioscience; CD133, Miltenyi; CD15, Biolegend; Sox2, Sino Biological Inc.; HIF1a, Biolegend; HIF2a, Abcam; and ENT1, Abcam) for 30 minutes at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Immunofluorescence staining was performed in 96 well plates on seminiferous tubules with primary antibodies major histocompatibility complex class II [MHC Class II RT1D (BioLegend, Clone OX-17, 205401, 1:200)] ...
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bioRxiv - Immunology 2021Quote: CD3+ T cells from lung single-cell suspensions (1×107 cells) were isolated by negative selection using the MojoSort mouse CD3+ T-cell Isolation kit (Biolegend) according to the manufacturer’s instructions ...