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Citations for BioLegend :
1301 - 1350 of 4924 citations for Recombinant Mouse CD28 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Frozen PBMC of post-CA patients were thawed and incubated in media (RPMI1640 + 2% FBS) with recombinant human Nectin-2 (Biolegend) or isotype control (ENZO ...
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bioRxiv - Immunology 2021Quote: ... The antigenicity and the immunogenicity of the purified recombinant spike protein was validated (Supplementary Fig 1) against verified available recombinant spike protein (BEI Resources, NR52397) and anti-SARS-CoV-2 spike antibody clone A20085C (BioLegend).
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bioRxiv - Neuroscience 2022Quote: ... neuronal activity was recorded for 15– 30 min daily before and after treatment with recombinant CXCL11 (200 nM, BioLegend #573606) using the Maestro Pro MEA System (Axion BioSystems) ...
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bioRxiv - Immunology 2022Quote: ... 5×104 B cells were first incubated with 5 µg/ml anti-IgM complexes and 1 µg/ml recombinant Human CD40L (Biolegend) for 30 mins and then 1×104 Tfr cells were added ...
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bioRxiv - Physiology 2023Quote: ... zoledronic acid (Novartis Pharmaceuticals) and/or 50 μg/kg (i.p.) recombinant human granulocyte-colony stimulating factor (G-CSF; carrier-free, BioLegend #578604) for three days ...
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bioRxiv - Physiology 2023Quote: Kupffer cells cultured in 96-well plates were supplemented with 30 μg EPO per well (recombinant rat EPO carrier-free, Biolegend). After 1,5 hours ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at 37°C and 5% CO2 for one hour to settle and then stimulated with 20 ng/mL recombinant IFNg (BioLegend), 100 ng/mL LPS-EB Ultrapure (Invivogen ...
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bioRxiv - Immunology 2023Quote: ... 200 μl for 96-well plates) supplemented with 1 μg/ml of recombinant human B cell-activating factor (BAFF, BioLegend). 1 µg/ml rHA from H1N1 (PR8 ...
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bioRxiv - Immunology 2022Quote: ... before staining with anti-mouse Granzyme B-efluor 450 and anti-mouse IFNγ-PE (BioLegend). Brain cells isolated from Pba-infected mice injected with BrdU were stained using the FITC BD BrdU Flow Kit (BD Pharmingen™ ...
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bioRxiv - Immunology 2019Quote: ... anti-mouse CD86 (GL1) and anti-mouse CD40 (Clone 3/23) were all from Biolegend.
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bioRxiv - Molecular Biology 2019Quote: ... FITC anti-mouse CD4 (100509) and FITC anti-mouse/human CD45R/B220 (103205) from BioLegend, Streptavidin PerCP-Cyanine5.5 (45-4317-82 ...
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bioRxiv - Systems Biology 2020Quote: ... while mouse spleen samples were incubated with PE anti-mouse TCR β chain antibody (BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... anti-mouse CD8 AlexFluor700 (cloneYTS156.7.7) and anti-mouse CD44 PerCP-Cy5.5 (clone IM7; all Biolegend) in PBS + 0.5% BSA + 0.05%NaN3 ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies used for mouse cells are rat anti-mouse CD8a conjugated to FITC (BioLegend, 100706) and rat anti-mouse CD19 conjugated to PE (BioLegend ...
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bioRxiv - Microbiology 2021Quote: ... 15 μg Dylight488-conjugated anti-mouse CD31 (LEAF purified anti-mouse CD31, clone 390, Biolegend, #102412 + DyLight488 Antibody Labelling Kit ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa594 anti-mouse Ly-6G/Ly-6C (Gr-1) antibody (BioLegend 108448, 10ug per mouse), rat Ultra-LEAF™ purified anti-mouse CD106 antibody ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa594 anti-mouse Ly-6G/Ly-6C (Gr-1) antibody (BioLegend 108448, 10ug per mouse) and PE rat anti-mouse CD62E (for E-selectin ...
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bioRxiv - Immunology 2023Quote: ... and different TotalSeq-C Anti-Mouse Hashtags (TotalSeq-C Anti-Mouse Hashtags 1-10 Biolegend #155861-155879 (Supplemental Tables 1 and 2) ...
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bioRxiv - Neuroscience 2021Quote: ... or monoclonal fluorescent conjugated antibodies (rat anti-mouse CD3, clone 17A2; mouse anti-mouse CD45.2, clone 104; all from Biolegend and used at 0.4mg/kg i.v.).
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bioRxiv - Immunology 2019Quote: After isolation cells were treated with Fc receptor blocking solution and stained with anti-CD14-APC (2 μg/ml, 61D3; Biolegend, San Diego, USA), anti-CD41-PE (2 μg/ml ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 µl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 μl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... Red blood cell lysis was performed and resulting leukocytes were resuspended in PBS + 1% BSA and blocked for 10 mins on ice with FC block and stained with Sytox® blue viability marker and conjugated antibodies anti-CD45-APC/Cy7 (Biolegend, 103115), anti-CD11b-APC (Biolegend ...
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bioRxiv - Neuroscience 2020Quote: ... Cell suspensions were incubated for 20 minutes on ice with anti-CD16/CD32 to block Fc receptors (1:50; BioLegend Cat #: 553141) and with a viability dye ...
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bioRxiv - Immunology 2020Quote: Cells were first incubated in 100 μL of PBS with 2mM EDTA for 15 min with 5μL Fc Block (Human TruStain FcX™, Biolegend, cat #422301) and a 1:500 dilution of Zombie UV viability dye (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... in FACS buffer for 30 minutes at 4°C followed by 2 wash steps with FC stain buffer and resuspended in Fluorofix (BioLegend, cat. 422101) for 30 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with Fixable Viability Stain 510 for 15 minutes at 4 °C followed by anti-Fc blocking reagent (Biolegend, Cat# 101320) for 10 minutes prior to surface staining ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted down at 1,500 rpm at 4°C for 5 min and cell pellets were resuspended in 100 uL FACS buffer (PBS + 4% FBS +0.5 mM EDTA) containing Fc block (BioLegend, 101302, 1:200). After 15 min incubation at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Nonspecific antigen binding to Fc receptors was inhibited via 10 minutes of blocking with Human TruStain FcX (BioLegend, San Diego, CA, USA). Cells were subsequently stained with mouse anti-human CD20 (Alexa-700) ...
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in 90 μl of 1% BSA in PBS and Fc blocked with 10 μl Human Trustain FcX (Biolegend cat. 422302) for 10 minutes on ice then stained on ice for 45 minutes with a pool of 268 antibodies in 100 μl ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and incubated in FACS buffer (1xPBS, 2%FCS) with the following anti-human antibodies: CD19-BV785 (BioLegend, 1:10), CD3-APC-eFluor 780 (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed twice and resuspended in PBS containing 2% FBS before staining in the presence of Fc block (Miltenyi 130-059-901) with anti-human CD45 Alexa700 (clone HI30, BioLegend cat #304024) and anti-mouse CD45.1 PE-Cy7 (clone A20 ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were anaesthetised as described above and intranasally inoculated with 100ng of carrier free recombinant protein (rIL-1α – Biolegend #575002, rIL-1β – Biolegend #575102) suspended in sterile phosphate buffered saline (PBS) ...
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bioRxiv - Cell Biology 2021Quote: ... non-adherent cells were moved to bacteriological Petri dishes the next day and differentiated over 1 week in cDMEM containing 20 ng/mL recombinant murine M-CSF (Biolegend, 576404). Generation of murine bone-marrow derived dendritic cells (BMDC ...
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bioRxiv - Microbiology 2020Quote: ... 5 × 105 cells/mL of CD14+ enriched cells were stimulated using recombinant human (rh) GM-CSF and rhIL-4 (BioLegend, USA) at a concentration of each 500 IU/mL in RPMI1640 media supplemented with 10% FCS at 37°C in 5% CO2 ...
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bioRxiv - Genomics 2022Quote: ... 0.2×106 naïve CD4+ T cells were cultured in presence of 10 ng/mL of recombinant IL-12 (BioLegend, Cat#577002), 1 ng/mL of recombinant IL-2 (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: For inflammatory activation colon fibroblasts were plated in 6-wells and after 24 hours treated with 1 ng/ml murine recombinant IL-1α (Biolegend, 575002) in PBS for the indicated time periods ...
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bioRxiv - Genetics 2023Quote: ... Recombinant Anti-AKT1 + AKT2 + AKT3 (phospho S472 + S473 + S474, Purified anti-Tubulin β-3 (TUBB3) Antibody 1:1000 (Biolegend, # PRB-435P), anti-GAPDH antibody 1:1000 (Sigma Aldrich ...
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bioRxiv - Immunology 2023Quote: ... For the memory B cell EliSpot, PBMC were stimulated with 1 µg/ml R848 (SigmaAldrich, SML0196) and 10 ng/ml recombinant human IL-2 (Biolegend, 791904) for 5 days before harvesting and plating of 50-100.000 cells/well ...
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bioRxiv - Cell Biology 2023Quote: ... breast cancer cells and myeloma cells were treated with recombinant hTRAIL (B294007, Biolegend, 1 to 300 ng/ml for different cells) for 3 h in the presence or absence of either HL-III (5 milliunits/ml) ...
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bioRxiv - Immunology 2022Quote: ... including mouse α-αM (clone ICRF44 for human experiments, clone M1/70 for mouse experiments, Biolegend), mouse anti-human/mouse α3 (α-α3 ...
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bioRxiv - Immunology 2020Quote: ... 116212) and T cell (PE rat anti-mouse CD4, Biolegend, 557308; FITC anti-mouse CD8a, Biolegend, 100706 ...
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bioRxiv - Immunology 2021Quote: ... APC-conjugated anti-mouse CD44 antibody and Pe-Cy7-conjugated anti-mouse CD62L antibodies (BioLegend, UK) were used for surface markers staining ...
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bioRxiv - Cell Biology 2022Quote: ... BV711 Mouse anti mouse CD64 (Monoclonal; clone X54-5/7.1; Biolegend; 1:100; Cat#139311, FACS). BV421 Rat anti mouse CD68 (Monoclonal ...
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bioRxiv - Genomics 2024Quote: ... 0.1μg mouse TotalSeq™-A HashTag and the mouse TotalSeq™-A universal cocktail v1.0 (Biolegend) diluted 1:4 ...
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bioRxiv - Microbiology 2020Quote: ... and goat anti-mouse HRP (Biolegend) at 1:10,000 in 2% BSA PBS-T for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-LAMP1 (H4A3, Biolegend, #328601), mouse anti-LAMP1 (H4A3 ...
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bioRxiv - Immunology 2021Quote: ... anti-mouse NKG2D-APC (130211, Biolegend) (dilution 1:100) ...
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PD-1 checkpoint blockade disrupts CD4 T cell regulated adaptive B cell tolerance to foreign antigensbioRxiv - Immunology 2021Quote: ... anti-mouse IgG1 (RMG1-1, BioLegend), polyclonal Hamster IgG (BioXcell) ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-HA (HA.11) (BioLegend), rabbit anti-HA (Bethyl ...