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Citations for BioLegend :
1101 - 1150 of 4924 citations for Recombinant Mouse CD28 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... low endotoxin (Super affinity) (Aero) followed by a secondary staining with APC conjugated anti-human IgG Fc Antibody (Biolegend). For cytotoxicity assay analysis ...
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bioRxiv - Immunology 2022Quote: ... before leukocytes were separated using 30 and 70% Percoll gradients and subsequently stained for 30 minutes in FACS buffer (PBS/2.5%FCS/10mM EDTA/0.01% NaN3) with the following primary antibodies: CD45-PacificBlue (Biolegend, 103126), CD19-biotin (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... CD141 BV605(Clone:M80 Cat:344117) in the presence of human anti-Fc Receptor antibodies (TrueStain; Biolegend; cat. no. 101320). cDCs1 were FACS-sorted as CD45+Lin- (Lin:CD3 ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed with LunaStain cell staining buffer (Polaris Biology, China) and first stained with Fc block (Biolegend, USA) for 10 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... approximately 200,000 cells per sample were resuspended in staining buffer (PBS, 2% BSA, and 0.01% Tween) and incubated for 10 minutes with Fc block (TruStain FcX (BioLegend) and FcR blocking reagent (Miltenyi) ...
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bioRxiv - Immunology 2023Quote: ... cells were stained for sorting in PBS containing 2% FCS at 4 °C with the following antibodies: CD271-PE (1:200; ME20.4, BioLegend), CD235a-PECy7 (1:20 ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated 20 min on ice with anti-CD16/32 (Fc-block, clone 93, BioLegend, San Diego, CA) then stained with the following antibodies directed toward cell surface receptors ...
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bioRxiv - Immunology 2023Quote: ... and resuspended in FACS buffer (PBS with 0.5% BSA and 2 mM EDTA) with Fc blocked using human TrueStain FcX (Biolegend) and incubated in the dark for 10 min at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... cells were washed with FACS buffer (PBS + 2% FBS + 0.05% NaN3) and incubated with Fc receptor blocking solution (Human TruStain FcX, BioLegend) for 5 minutes ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently resuspended in staining buffer (PBS with 0.5% bovine serum albumin and 2 mM EDTA) and Fc-blocked using TruStain FcX reagent (BioLegend). Cells were then stained with the following fluorophore-conjugated anti-mouse antibodies for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in FACS buffer (PBS with 0.5% BSA and 2 mM EDTA) and Fc blocked using human TrueStain FcX (Biolegend). For cell surface staining ...
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bioRxiv - Immunology 2023Quote: ... Roughly 0.5 x 106 cells were washed in FACS buffer (PBS + 3% FCS + 0.1% sodium azide) and stained with either FITC-coupled anti-FcεR1 (1:100, clone MAR-1, BioLegend, #134306) and PE-coupled anti-CD117 (1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... Osaka University) were pre-incubated with anti-human-Fc secondary antibodies fused to APC (BioLegend, #410712, dilution 1:100) for 1 h in 1% BSA in PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... then resuspended in ice-cold MACS buffer (DPBS + 0.5% BSA + 2mM EDTA) and incubated with Fc Block (Biolegend 156603) for 5 min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... one million cells were first resuspended in 100 μL buffer containing 5 μL Fc Receptor Blocking Solution (Biolegend, #422302) and incubated for 15 minutes ...
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bioRxiv - Immunology 2023Quote: ... and single cell preparations were obtained by passing the cell mixture through a PluriStrainer (500 – 70µM cell strainers; 43-50500, PluriSelect) before blocking with Fc block for 10 min (422302, BioLegend). CD45+ cells were enriched using anti-CD45 magnetic MicroBeads and LS columns (130-045-801 and 130-042-401 ...
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bioRxiv - Developmental Biology 2024Quote: ... 45 µL of cell suspension was transferred to a new 1.5 mL Eppendorf tube (approximately 350,000 cells) and 5 µL of Human TruStain FcX™ Fc Blocking reagent (Biolegend) were added to block unspecific binding of the hashing antibodies ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were thawed and staining for surface antigens was performed following Fc receptor blockade with Human TruStain FcX (BioLegend). Central/effector memory CD4+ T cells were defined as live/CD16-/CD14-/CD123-/CD56-/CD11c-/CD19-/CD4+ > excluding CD25hiCD127lo > CD45RA-/CD27+ ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then blocked on ice with PBS + 7.5% FCS for 15 minutes before staining with antibody cocktails (see key resource table) containing TruStain Monocyte Blocker (Biolegend) in FACS buffer for 45 minutes at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Barcoded cells were combined into a composite sample before incubation in Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) for 10 min at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... rabbit or mouse anti-Tubb3 (TUJ1, Abcam rabbit 52623, Biolegend mouse 801201), mouse anti-Citron kinase (CITK ...
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bioRxiv - Immunology 2020Quote: ... Bound mouse IgG was detected with a mouse IgG-specific secondary (Biolegend). For avidity measurements ...
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bioRxiv - Immunology 2022Quote: ... anti-mouse APC/Cyanine7 mouse CD107a Antibody (Clone 1D4B; BioLegend, Cat#: 121616); anti-CD184 (CXCR4 ...
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bioRxiv - Immunology 2023Quote: ... BV605 Mouse Anti-Mouse CD45.1 (Clone A20; BioLegend; Cat#110738; RRID: AB_2562565) BUV395 Mouse Anti-Mouse CD45.2 (Clone 104 ...
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bioRxiv - Immunology 2024Quote: ... and anti-mouse IgD (Alexa Fluor® 647 anti-mouse IgD, Biolegend) was applied onto the section for 2 h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... and PE/Fire 810 mouse anti-mouse CD161 (NK1.1) (BioLegend Cat# 156535) Gating strategies for flow cytometry are described in Extended Data Fig ...
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bioRxiv - Physiology 2024Quote: ... or mouse anti-mouse NK1.1-APC/Fire750 (clone PK136, cat# 108752, BioLegend), and rat anti-mouse/human B220-APC/Fire810 (clone RA3-6B2 ...
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bioRxiv - Systems Biology 2020Quote: ... respective concentrations (ranging from 25 ng/mL to 100 ng/mL) of recombinant human IL-8 (Biolegend, 715404) and/or recombinant human CCL2 (Peprotech ...
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bioRxiv - Cell Biology 2022Quote: ... Bone marrow cells were differentiated into BMDMs using 40 ng/mL recombinant M-CSF (BioLegend, catalog number 576406) in the same media formulation as TR-AMs ...
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bioRxiv - Microbiology 2024Quote: ... Activation of MAPK and NF-κB signaling pathways was triggered using human recombinant TNF-α (BioLegend, Cat. 570102). Detailed information concerning experimental procedure is given in supplementary material.
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bioRxiv - Immunology 2024Quote: ... and 10 ng/ml recombinant macrophage colony-stimulating factor (M-CSF; Cat# 576402; BioLegend, San Diego, CA, USA) for 7 days ...
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bioRxiv - Cell Biology 2020Quote: ... mouse CD31 (APC, Biolegend), mouse Ter119 (APC ...
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bioRxiv - Immunology 2021Quote: ... mouse CD4-PE (Biolegend), human CD4-APC (Biolegend) ...
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bioRxiv - Immunology 2020Quote: ... α-mouse PD1-APC (BioLegend), α-mouse Ly6G-VioBlue (TONBO Biosciences) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... anti-mouse CD19 (Biolegend, cat# 115546 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... anti-mouse CD3 (Biolegend, cat#100236 ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse CD31 (APC, Biolegend), mouse Ter119 (APC ...
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bioRxiv - Microbiology 2020Quote: ... mouse HA (BioLegend, #901515), rabbit SQSTM1/p62 (Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: ... TMEM119 (mouse primary, Biolegend, 853302 ...
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bioRxiv - Microbiology 2023Quote: ... Mouse-anti-GFP (Biolegend), Mouse-anti-H2BK120ub (Active Motif) ...
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bioRxiv - Microbiology 2023Quote: ... mouse CD45.1-AlexaFluor647 (BioLegend) were diluted 1:300 in 2% BSA in PBS and used for primary staining of the cells overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-HA (mouse, Biolegend) and anti-KPNA3 (rabbit ...
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bioRxiv - Microbiology 2023Quote: ... Mouse-anti-SSRP1 (Biolegend), Mouse-anti-GFP (Biolegend) ...
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bioRxiv - Microbiology 2023Quote: ... Mouse-anti-Spt16 (Biolegend), Mouse-anti-SSRP1 (Biolegend) ...
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bioRxiv - Cancer Biology 2023Quote: ... mouse CD45 (BioLegend #103130), human CD8 (BioLegend #344712) ...
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bioRxiv - Bioengineering 2022Quote: ... anti mouse CD90 (Biolegend), anti-mouse VCAM-1 (eBioscience ...
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bioRxiv - Immunology 2023Quote: ... and mouse (Biolegend, #401402) antibodies were used ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse CD45 (BioLegend #103130), human CD45 (BioLegend #304014) ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-mouse MHCII (Biolegend) and anti-mouse CD11b (eBioscience) ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-mouse CD206 (Biolegend), anti-mouse MHCII (Biolegend ...