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Citations for BioLegend :
1151 - 1200 of 4924 citations for Recombinant Mouse CD28 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... anti-mouse CD3 (Biolegend), anti-mouse CD206 (Biolegend) ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-mouse B220 (Biolegend), anti-mouse CD3 (Biolegend) ...
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bioRxiv - Immunology 2019Quote: ... anti-mouse CD107b (Mac-3) and anti-mouse TNF (MP6-XT22) (all Biolegend). Live-Dead Fixable Green and Live-Dead Fixable Aqua Dead Cell stains was purchased from Thermofisher (Boston ...
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bioRxiv - Microbiology 2021Quote: ... APC mouse anti-mouse CD64 (Clone X54-5/7.1, Biolegend, San Diego, CA), APC-Cy7 rat anti-mouse CD11b (Clone M1/70 ...
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bioRxiv - Immunology 2021Quote: Indicated mouse cytokines were measured using the LEGENDplex Mouse Macrophage/Microglia Panel (BioLegend). Samples were prepared according to manufacturer's instructions and analysed by flow cytometry ...
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bioRxiv - Immunology 2021Quote: ... rat anti-mouse IL-6 or rat anti-mouse Ly6G (all from Biolegend), followed by fluorescence-conjugated secondary antibodies ...
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bioRxiv - Bioengineering 2023Quote: ... Isolated cells were mouse (TruStain FcX, anti-mouse CD16/32, clone 93, Biolegend) and human (TruStain FcX ...
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bioRxiv - Neuroscience 2023Quote: ... rat purified anti-mouse CD49d antibody (For VLA-4α, BioLegend 103701, 1µg/mouse), rat IgG2b κ control antibody (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Rat purified anti-mouse CD11a antibody (For LFA-1α, BioLegend 101101, 1µg/mouse), rat purified anti-mouse CD49d antibody (For VLA-4α ...
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bioRxiv - Bioengineering 2024Quote: ... FITC anti-mouse CD8a antibody and APC/Cyanine7 anti-mouse CD4 antibody (BioLegend) in PBS ...
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bioRxiv - Physiology 2024Quote: ... mouse anti-mouse CD64-PE/Dazzle594 (clone X54-5/7.1, cat# 139320, BioLegend), rat anti-mouse PDGFRα-PE/Cy5 (clone APA5 ...
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bioRxiv - Biochemistry 2020Quote: ... incubated with secondary antibody (1/100 dilution of chicken anti-HIS-FITC polyclonal from Immunology Consultants Laboratory; or 1/250 anti-human IgG-APC clone HP6017 from BioLegend) for 30 minutes on ice ...
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bioRxiv - Cell Biology 2020Quote: ... before plating the cells in petri dishes in RPMI 1640 medium containing 10 % heat-inactivated FCS and 50 ng/ml M-CSF (BioLegend) or 50 ng/ml GM-CSF (BioLegend) ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: Cells were incubated with anti-CD16/32 (Fc block) prior to staining with the following fluorophore or biotin conjugated monoclonal antibodies purchased from Biolegend, eBioscience ...
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bioRxiv - Immunology 2021Quote: ... cells were washed twice in FACS buffer and incubated with rat anti-human Fc antibody conjugated to allophycocyanin (Cat: 410712, BioLegend) for 15 minutes on ice ...
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bioRxiv - Microbiology 2021Quote: ... the cells were incubated in PBS-5% FCS on ice for 30 min with rabbit anti-K10 (1:1000) (Biolegend), and K10 was visualized with anti-rabbit AF488 (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... Human B cells were incubated in human Fc receptor blocking antibody then stained with Zombie Aqua fixable viability dye (1:500 dilution) (BioLegend), according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: For analysis of LILRB2 expression on marmoset and human monocyte derived macrophages were first blocked with human TruStain FcX Fc blocking solution (Biolegend), then labelled with and without anti-human LILRB2 (5 µg/mL ...
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bioRxiv - Systems Biology 2020Quote: ... The cells were incubated with each antibody for 45 min at −22–25°C after FcR blocking using Fc blocker (Human TruStain FcX, Biolegend) or anti-mouse CD16/CD32 antibody ...
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bioRxiv - Microbiology 2021Quote: ... Cells were filtered through a 70 µm strainer and red blood cells were lysed before blocking FC receptors using anti-CD16/CD32 (BioLegend). Cell-specific proteins were labeled with the following antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were blocked for 10 min on ice following addition of 10 μL Fc blocking solution (BioLegend, cat. no. 422301), 4 μL of a 1% dextran sulfate solution (Research Products International ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and resuspended in 100 μL Binding Buffer containing 100 nM M1 anti-FLAG antibody labeled with Alexa Fluor 488 and Alexa Fluor 647 anti-human IgG Fc (BioLegend) diluted 1:100 (v/v) ...
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bioRxiv - Immunology 2022Quote: ... T cells were washed in PBS/1% FCS and stained using the following mAbs in appropriate combinations: anti-OX40-PE-Cy7 (BioLegend), anti-GITR- BV421 (BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... and the DAXL-88 (anti-AXL) binding assay used Alexa Fluor 647 AffiniPure anti-Fc tag Goat Anti-Human IgG (410711, BioLegend). Anti-Human CD4 antibody (BD ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were then washed once with ice-cold FACS buffer and incubated with 2 μg/mL PE anti-human IgG Fc (BioLegend) at 4 °C with gentle shaking in the dark for 1 hour ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were washed once with ice-cold FACS buffer and incubated with 1 μg/mL PE anti-human IgG Fc (Biolegend) at 4 °C with gentle shaking in the dark for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was discarded and the cell pellets were resuspended in 10% FCS in PBS containing Alexafluor647 anti-Ki67 (Biolegend; 1:200 in binding buffer ...
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bioRxiv - Immunology 2022Quote: The collected BMDCs were centrifuged once and resuspended in cell staining buffer (PBS supplemented with 2% FBS) containing anti-Fc receptor mAb (Biolegend). Cells were blocked for 10 min at 4°C ...
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bioRxiv - Immunology 2019Quote: ... cells were washed once in in 1ml of PBS-1%FCS (8’, 350rcf).Cells were then separately labeled with hashtag antibodies (Biolegend TotalSeq-A antibodies ...
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bioRxiv - Immunology 2021Quote: ... resuspended in 100 µL PBE with a 1:100 dilution of biotin anti-human IgG Fc antibody (clone HP6017, BioLegend) for human serum or plasma derived antibodies or a 1:25 dilution of biotin goat anti-rat or anti-mouse IgG antibody (A16088 ...
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bioRxiv - Immunology 2020Quote: ... for 30’ at RT in PBS containing 2% FCS and 1 mM EDTA prior to secondary staining with streptavidin-BrilliantViolet 510 (Biolegend) for 15’ on ice ...
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bioRxiv - Immunology 2020Quote: ... then cells were washed with 2% FCS-PBS and surface-stained with anti-human HLA-DR-Pacific Blue or -APC (clone L243; Biolegend) and anti-human CD123-FITC or -PeCy5 (clone 6H6 ...
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bioRxiv - Immunology 2021Quote: ... Single cell suspensions were blocked with rat anti-mouse FcγIII/II receptor (CD16/CD32) blocking antibodies (“Fc-Block”) and stained with live/dead cell-exclusion dye (Zombie UV dye; Biolegend). The cells were then incubated with fluorophore-conjugated antibodies directed against cell surface antigens ...
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bioRxiv - Immunology 2021Quote: ... Cells were then blocked with human Fc-block (Miltenyi) in the presence or absence of purified anti-CD36 antibody (Clone 5-271; Biolegend) at 10 μg/ml for a further 15 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 million cells were resuspended in 1 mL of FACS buffer and 50 μL of Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) was added and left to bind the cells on ice for 15 minutes ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and resuspended in 100 μL Buffer B containing 100 nM M1 anti-FLAG antibody labeled with Alexa Fluor 488 (In house) and Alexa Fluor 647 anti-human IgG Fc (BioLegend) diluted 1:100 (v/v) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice and resuspended for 30 minutes on ice in 1/250 anti-human IgG1-Fc-FITC (clone HP6017, BioLegend) and 1/1000 anti-hemagglutinin (HA ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in PBS containing 2% FCS at 4°C with the following antibodies: CXCR1-PerCP (1:50; 8F1/CXCR1, BioLegend), CD11b-PE (1:100 ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in PBS containing 2% FCS at 4°C with the following antibodies: CXCR1-FITC (1:100; 8F1/CXCR1, BioLegend), CD19-PerCP (1:50 ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in PBS containing 2% FCS at 4°C with the following antibodies: CD235a-PECy7 (1:20; HI264, BioLegend), CD31-APCCy7 (1:20 ...
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bioRxiv - Immunology 2022Quote: ... 2 % FCS (1 to 5 x107 cells/500 μl) and incubated with the following mAbs: CD45 AF700 (Biolegend, clone HI30), CD34 PB (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... cells were stained for sorting in PBS containing 2% FCS at 4°C with the following antibodies: CD138-FITC (1:20; MI15, BioLegend), CD45-PerCP-Cy5.5 (1:20 ...
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bioRxiv - Immunology 2022Quote: ... Cells were stained with a staining mix containing Fc receptor blocker (TruStain fcX™, BioLegend, Amsterdam, The Netherlands, 1:100), viability stain (Zombie Aqua™ and Zombie Green™ Fixable Viability Kit ...
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bioRxiv - Immunology 2022Quote: ... 2 % FCS (1 to 5 x107 cells/500 μl) and incubated with the following mAbs: CD45 AF700 (Biolegend, clone HI30), CD34 PB (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed in FACS stain buffer and resuspended in diluted Human TruStain FcX Fc blocking reagent according to manufacturer’s instructions (Biolegend, USA) at 4□°C for 10 minutes protected from light ...
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bioRxiv - Immunology 2023Quote: ... Samples were then split into 7 FACS tubes and incubated with 100uL of diluted Human TruStain FcX Fc blocking reagent according to manufacturer’s instructions (Biolegend, USA) at 4□°C for 10 minutes protected from light ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated with anti-CD16/CD32 (clone 93, eBioscience, 14-0161-86) for Fc receptor blockade and stained with Zombie Yellow (Biolegend) for 10 minutes at room temperature in the dark to distinguish live and dead cells ...
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bioRxiv - Genomics 2023Quote: ... Cells were pre-incubated with 5 µl of Human TruStain FcX™ (Fc Receptor Blocking Solution, BioLegend Cat. No. 422301) per 100µl of cell suspension for 5–10 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Cells were centrifuged at 400 g for 8 minutes and stained with lanthanide-labeled antibodies against immunophenotypic markers in CyTOF buffer containing Fc receptor blocker (BioLegend) for 30 minutes at room temperature (RT) ...
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bioRxiv - Immunology 2023Quote: ... were resuspended in labeling buffer (1% BSA in PBS) and underwent Fc blocking for 10 minutes on ice using Human TruStain FcX (BioLegend). Next ...