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Citations for BioLegend :
1201 - 1250 of 4924 citations for Recombinant Mouse CD28 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... single cell suspensions were incubated on ice for 10 minutes with Fc receptor-blocking anti-CD16/32 (BioLegend Cat# 101301). Cell suspensions were first stained for 5 minutes at room temperature with LIVE/DEAD® Fixable Red Dead Cell Stain Kit (ThermoFisher Cat# L23102) ...
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bioRxiv - Immunology 2023Quote: ... naïve CD8+ T cells were cultured in RPMI (10% FCS) and stimulated with plate-bound mCD3 mAbs (3 µg ml-1, clone 145-2C11, Biolegend), soluble mCD28 (0.5 µg ml-1 ...
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bioRxiv - Immunology 2023Quote: All flow cytometry was performed at Fred Hutchison Cancer Center utilizing a 28-color panel focused on T cell phenotyping (Supplementary Table 1) [33] CVC and PBMC were incubated with Fc-blocking reagent (BioLegend) and fixable UV Blue Live/Dead reagent (ThermoFisher ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were washed once with PBS and incubated in 50 µL PBS containing human Fc block (Supplementary Table 1) and Zombie Aqua viability dye (1:200, BioLegend) for 15 min at 4°C ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with FACS staining buffer (PBS containing 2% fetal bovine serum [FBS] and 1 mM EDTA) and incubated with an Fc receptor blocker (TrueStain fcX; BioLegend) for 5 minutes at 4℃ ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed and stained for 30min at 4°C in the dark with Fc block diluted 1:200 and subsets of the following antibodies: anti-CD103 (2E7, Biolegend), anti-CD11b (M1/70 ...
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bioRxiv - Cancer Biology 2022Quote: ... or TFH mimicking signals (including 0.02 μg/mL recombinant human IL-4, Peprotech; 0.05 μg/ml recombinant human IL-21, Peprotech, and 5 μg/mL anti-human CD40, Biolegend). Complete RPMI medium contained RPMI 1640 (Gibco ...
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bioRxiv - Immunology 2022Quote: ... neutrophils were incubated with autologous activated platelets (1:10 ratio) (65) for 2.5h and human recombinant CCL28 (50 nM) (BioLegend), the CCR3 antagonist SB328437 (10 mM ...
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bioRxiv - Pathology 2022Quote: ... or B cell supernatant pre-treated with a recombinant antibody to deplete IL-10 (IgG2b, clone JES5-16E3, Biolegend). As controls ...
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bioRxiv - Cancer Biology 2022Quote: Cells cultured in 2D were seeded at 5 × 105 cells/well in 6-well plates and incubated with recombinant human interferon gamma (IFNγ) (0.5 ng/mL) (Biolegend) and/or recombinant tumour necrosis factor alpha (TNFα ...
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bioRxiv - Immunology 2023Quote: ... Human Th17 differentiation assays were performed similarly except the concentrations of recombinant human IL-6 (30 ng/ml, BioLegend), TGF- β1 (2.25ng/mL) ...
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bioRxiv - Immunology 2024Quote: ... KO1, KO2) and treatment conditions (no IFN, IFN-β, IFN-λ) were incubated with separate oligonucleotide-tagged antibodies (HTO) for multiplexing (Biolegend TotalSeq, A0301 - A0309). Groups were counted and pooled in equal abundance ...
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bioRxiv - Bioengineering 2022Quote: ... HRP-conjugated anti-mouse secondary HRP goat anti-mouse IgG antibody (#4053, Biolegend, USA) (1:5000 in TBST ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were stained using PerCP anti-mouse CD3 and APC anti-mouse CD4 (Biolegend) for 20 min at room temperature ...
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Accumulation of TCR signaling from self-antigens in naive CD8 T cells mitigates early responsivenessbioRxiv - Immunology 2023Quote: ... the following biotinylated anti-mouse or anti-mouse/human antibodies were purchased from BioLegend: CD4 (clone RM4-5) ...
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bioRxiv - Cancer Biology 2023Quote: ... mouse anti-human/mouse Granzyme B conjugated to PerCP/Cyanine5.5 (clone QA16A02, Biolegend, 372211), mouse anti-human Perforin conjugated to PE/Cyanine7 (clone B-D48 ...
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bioRxiv - Cell Biology 2019Quote: ... purified mouse IgG1(401401, Biolegend); 14C10 ...
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bioRxiv - Immunology 2020Quote: ... α-mouse PD-L1-PE (BioLegend), α-mouse PD1-APC (BioLegend) ...
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bioRxiv - Immunology 2020Quote: Mouse α-PD-L1 (BioLegend), α-mouse PD-L1-PE (BioLegend) ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... anti-mouse CD4 (APC, Biolegend) and anti-mouse CD8a (PE ...
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bioRxiv - Developmental Biology 2022Quote: ... mouse anti-SMA (Biolegend; 904601), rabbit anti-Sox9 (Millipore ...
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bioRxiv - Immunology 2021Quote: ... or mouse G-CSF (BioLegend). The blocking of H3 and G-CSF was performed by preincubating the different 3% plasma with 0.5 µg/ml of anti-hH3 ...
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bioRxiv - Cancer Biology 2020Quote: ... VE-Cadherin (mouse, 348502; BioLegend), CD63 (mouse ...
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bioRxiv - Developmental Biology 2019Quote: ... and α-mouse PDGFRα (BioLegend). Data were acquired on LSR-Fortessa flow cytometer (BDbiosciences ...
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bioRxiv - Immunology 2021Quote: ... anti-mouse EPCAM-PECy7 (BioLegend), anti-mouse Ly6G-Alexa Fluor 700 (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... anti-mouse CD11c-PerCPCy5.5 (BioLegend), anti-mouse CD11b-APC-Cy7 (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... anti-mouse MHCII (107601, Biolegend), and anti-mouse E-cadherin (ab76319 ...
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bioRxiv - Bioengineering 2020Quote: ... BV785 anti-mouse CD11b (BioLegend), AF532 anti-mouse CD8a (Invitrogen) ...
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bioRxiv - Bioengineering 2020Quote: ... AF647 anti-mouse CD40 (BioLegend), APC-R700 anti-mouse Ly-6G (BioLegend) ...
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bioRxiv - Bioengineering 2020Quote: ... BV510 anti-mouse CD19 (BioLegend), BV570 anti-mouse CD3 (BioLegend) ...
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bioRxiv - Bioengineering 2020Quote: ... BV570 anti-mouse CD3 (BioLegend), BV650 anti-mouse F4/80 (BioLegend) ...
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A novel neural stem cell-derived immunocompetent mouse model of glioblastoma for preclinical studiesbioRxiv - Cancer Biology 2020Quote: ... mouse anti-GFAP (644701, BioLegend). Secondary antibodies were ...
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bioRxiv - Synthetic Biology 2021Quote: ... and mouse anti-HA (BioLegend) antibodies ...
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bioRxiv - Microbiology 2020Quote: ... α-mouse CD4 BV-650 (Biolegend) and α-mouse CD44 FITC (BD BioSciences ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse α-HA (Biolegend #901513), Mouse α-PDI (Affinity Bioreagents #MA3-018) ...
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bioRxiv - Cell Biology 2022Quote: ... or mouse IgG1 (BioLegend, 400102) was added and samples were incubated for 1 hour at 4°C under rotation ...
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bioRxiv - Microbiology 2020Quote: ... or mouse-anti-CD34 (Biolegend), all diluted 1:100 in antibody dilution buffer (PBS containing 1% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2020Quote: ... Anti-HA mouse antibody (BioLegend) was adsorbed to protein G agarose beads and incubated with the cell lysates for 2h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... α-mouse CD4 BV-650 (Biolegend), α-mouse Ly-6C APC-Cy7 (BD BioSciences ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse anti-HA (901501, Biolegend), rabbit anti-HDAC1 (#2062 ...
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bioRxiv - Neuroscience 2020Quote: ... anti-sAPPα (mouse, 6E10, BioLegend), anti-human APP770 (rabbit ...
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bioRxiv - Developmental Biology 2022Quote: ... FITC anti-mouse CD41 (Biolegend), PE rat anti-mouse CD43 (BD Bioscience ...
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bioRxiv - Pathology 2022Quote: ... A4G8 (1/500, mouse, Biolegend), AT8 (1/1000 ...
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bioRxiv - Immunology 2022Quote: ... anti-mouse CD45.2-PE (BioLegend), hamster anti-mouse CD3e-FITC (BD Biosciences) ...
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bioRxiv - Immunology 2019Quote: ... and anti-mouse IgE (BioLegend), respectively ...
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bioRxiv - Cell Biology 2019Quote: ... Mouse mAb anti-GFP (BioLegend), mouse mAb anti-c-myc (9E10 ...
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bioRxiv - Microbiology 2021Quote: ... FITC anti-mouse CD8 (BioLegend) for CD3 ...
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bioRxiv - Immunology 2019Quote: ... Biotinylated mouse anti IgG2A (Biolegend) served as the isotype control ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-HA 16B12 (Biolegend), rat anti-HA (Roche) ...
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bioRxiv - Immunology 2020Quote: ... Anti mouse Lambda (Biolegend, #407306) were used to define different cellular populations ...