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Citations for BioLegend :
1101 - 1150 of 7287 citations for 7H Pyrazolo 4 3 d pyrimidin 7 one 1 4 dihydro 3 b D ribofuranosyl oxime monohydrochloride 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... then stained 15 minutes for surface markers and fixed for 20 minutes with 4% paraformaldehyde (PFA) in PBS (BioLegend). Cells were then permeabilized with 0.5 % saponin in Running buffer and stained for intracellular markers ...
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bioRxiv - Molecular Biology 2020Quote: ... 600 μL of WCE was incubated overnight at 4°C with 10μg of monoclonal RNA Poll antibody (Biolegend, USA), 2μg of anti-myc antibody (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... Th1 cells were differentiated for 4 days in the presence of plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-12 (25 ng/ml ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Tissue was then blocked with 10% normal donkey serum/0.1% Triton X- 100/0.01% NaN3 followed by staining with Alexa Fluor 488 conjugated anti-human CD4 OKT 4) antibody (Biolegend) at 4° Celsius overnight (1:200 diluted in PBS) ...
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bioRxiv - Immunology 2020Quote: ... stained for 20 min at 4 °C in PBS/2% FCS in presence of Fc receptor blocking reagent (Biolegend) with anti-murine CD326-BV605 ...
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bioRxiv - Microbiology 2021Quote: ... Primary CD4+ T cells were activated for 4-5 days on T25 flasks coated with 5μg anti-CD3 antibody (clone OKT3, Biolegend) in the presence of 2μg/ml soluble anti-CD28 antibody (clone CD28.2 ...
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bioRxiv - Immunology 2020Quote: The following antibodies and reagents were used for flow cytometry: CD4 clone RM-4-5 (BD Biosciences, BioLegend, Tonbo), CD8α clone53-6.7 (BD ...
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bioRxiv - Immunology 2020Quote: ... Sections were stained at 4°C on an agitation stage overnight with anti-CD169 and anti-Ly6G antibodies (BioLegend). Stained sections were microscopically analyzed using Leica SP8 confocal microscope (Leica Microsystem ...
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bioRxiv - Cancer Biology 2022Quote: ... we collected the beads and labeled them with 4 μg/ml PE-labeled anti-CD63 antibody (BioLegend, clone H5C6) for 45 min at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... Samples were washed in PBS and were incubated at 4°C with CD16/32 Fc block (cat#101302, BioLegend) in PBS for 10 minutes before staining with antibodies (Supplementary Table 1 ...
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bioRxiv - Immunology 2022Quote: Human cells were surface stained for 25 minutes at 4 °C with antibodies to with anti-CD3 (Clone UCHT1, eBioscience) and anti-CD56 (Clone 5.1H11, Biolegend).
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were stained 30min at 4°C in the dark with PerCP/Cyanine5.5 anti-mouse Ly6G (clone:1A8, cat. no. 127615, BioLegend), FITC anti-mouse/human CD11b (clone:M1/70 ...
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bioRxiv - Neuroscience 2023Quote: ... and stained for intracellular cytokines (IFN-γ PE #505808, IL-4 BV421 #504120, IL-17A PE-Cy7 #506922, Biolegend) with the Cyto-Fast™ Fix/Perm Buffer Set (#426803 ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in the dark for 15 min at 4°C with fluorochrome-conjugated antibodies in PBS containing anti-CD16/CD32 (2.4G2; BioXcell or BioLegend). Cells were washed and ...
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bioRxiv - Systems Biology 2022Quote: Sorted live single cells were centrifuged at 400 x g for 5min at 4°C and resuspended in 25μL of cell staining buffer (BioLegend). Human TruStain FcX FC blocking reagent (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were incubated in primary antibody in 0.1 M Tris/2% FBS in a humidified chamber overnight at 4°C (SMI-32, Biolegend 801702 ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was discarded and cells were stained for 10 min at 4 °C with 2.5 μl Human TruStain FcX block (BioLegend) and 22.5 μl FACS buffer ...
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bioRxiv - Cell Biology 2023Quote: ... tumor cell phenotyping and mouse blood phenotyping were evaluated with the following anti-human antibodies: CD155 (SKII.4, Biolegend), CD19 (HIB19 ...
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bioRxiv - Genomics 2024Quote: ... The sorted cells were centrifuged at 400xg for 5mins at 4°C and re-suspended in 25μl Cell Staining Buffer (BioLegend). 2.5μl of 1:10 TruStain FcX™ PLUS (antimouse CD16/32 ...
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bioRxiv - Immunology 2024Quote: ... B cells were isolated using the EasySep Human B Cell Isolation Kit (StemCell #17954) or the MojoSort Human Pan B Cell Isolation Kit (BioLegend #480082) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Viability of the cells was >90% (7-AAD, Biolegend), and purity for CD41a+ CD42b- was >85% and CD41a+ CD42b+ was >95% ...
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bioRxiv - Genomics 2021Quote: ... anti-IL-2 (MQ1-17H12; PE Cy-7; Biolegend), and anti-MIP-1β (D21-1351 ...
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bioRxiv - Bioengineering 2021Quote: ... APC/Cyanine 7 anti-human CD8 antibody (#344714, Biolegend). Population of Annexin V+and 7AAD+ Raji cells obtained by gating out T cells (CD4+/CD8+ ...
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bioRxiv - Bioengineering 2021Quote: ... APC/Cyanine 7 anti-human CD8 antibody (#344714, Biolegend). Total apoptosis of Raji cells was obtained by gating for 7AAD+ and Annexin V+ population after gating out T cells (CD4+CD8+) ...
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bioRxiv - Bioengineering 2021Quote: ... PE/Cyanine 7 anti-human CD4 antibody (#317414, Biolegend), APC/Cyanine 7 anti-human CD8 antibody (#344714 ...
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bioRxiv - Bioengineering 2020Quote: ... and F4/80 (APC/Cyanine 7, BioLegend catalog# 123117). All antibodies were diluted 1:200 ...
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bioRxiv - Immunology 2022Quote: ... B220 (#103235) and GL-7 (#144617) purchased from Biolegend. GC B cells were sorted on a BD Aria III FACs sorter (Beckton Dickinson ...
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bioRxiv - Microbiology 2022Quote: ... Cells were stained with 7-AAD for viability (Biolegend).
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bioRxiv - Immunology 2020Quote: ... the exclusion of 7-AAD and Annexin V (BioLegend) was assessed at various time points via flow cytometry per manufacturer recommendations.
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bioRxiv - Immunology 2019Quote: ... CD8a APC-Cyanine 7 (APC-Cy7, clone: HIT8a, Biolegend), PD-1 phycoerythrin (PE)-Cy7 (clone EH12.2H7 ...
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bioRxiv - Immunology 2019Quote: ... 7-AAD or Zombie Aqua fixable viability dye (Biolegend). Fluorescence-minus-one controls confirmed gating strategies ...
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bioRxiv - Microbiology 2022Quote: ... and 5 μL 7-AAD Viability Staining Solution (Biolegend). After incubation ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cell viability dyes used were 7-AAD (Biolegend; 420404) or Sytox blue (Fisher Scientific ...
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The loss-of-function of SOCS2 increases the inflammatory response to Staphylococcus aureus infectionbioRxiv - Immunology 2023Quote: ... Cell viability was determined using 7-AAD dye (Biolegend). The amount of live (GFP+ 7AAD- ...
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bioRxiv - Immunology 2023Quote: ... 5 μL of 7-AAD (50 µg/mL, BioLegend) or helix green (1 nM final concentration ...
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bioRxiv - Microbiology 2023Quote: ... anti-CD8 (PE-Cy-7, 53-6.7, BioLegend 100721), anti-FoxP3 (AF532 ...
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bioRxiv - Immunology 2024Quote: ... PE/Cyanine 7 anti-mouse CCR7 (clone 4B12, BioLegend), Brilliant Violet 786 anti-mouse CD3e (clone 145-2C11 ...
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bioRxiv - Immunology 2023Quote: ... or a combination of 100ng/ml IL-7 (BioLegend) and 100ng/ml IL-18 (BioLegend ...
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bioRxiv - Biochemistry 2022Quote: ... the transferred membrane was blocked with Blocking One and incubated with HRP-linked streptavidin (BioLegend, #405210, 1:10,000) in TBS-T containing 5% Blocking One for 1 h ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated with PE-streptavidin (BioLegend, 405204), SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Cell Biology 2020Quote: ... T- and B-cell depleted cultures were incubated with antibodies for CD34 (clone 561, PE conjugate, BioLegend, 1:100) prior to sorting ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated to PE-streptavidin (BioLegend, 405204), SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Cancer Biology 2022Quote: ... about 1-2e6 mononuclear cell suspension was stained with a panel of Total-seq B antibodies (1ug/each, Biolegend) and fluorochrome conjugated flow antibodies for CD45 (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... each sample was labelled with 0.1 ug of a cell hashing antibody (TotalSeq-B anti-human Hashtag antibodies 1 through 8, BioLegend). Cells were labelled for 30 minutes on ice in the dark ...
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bioRxiv - Immunology 2021Quote: ... plates were coated at least 5 hours prior to use with 3 ug/ml plate-bound anti-CD3 antibody (clone OKT3; BioLegend, LEAF grade), 1 ug/ml soluble anti-CD28 antibody (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... Cytokine staining of splenocytes after attLm-OVA infection was carried out after re-stimulation of CD8 T cell enriched splenocytes with 50 nM SIINFEKL peptide for 3 hours with Brefeldin A (Biolegend, cat#:420601).
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected 3 days after coculture initiation and used to quantify T cell activation levels with ELISA (BioLegend, Cat no. 430801) following the manufacturer’s protocols.
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bioRxiv - Immunology 2024Quote: ... Primary lymphocytes isolated from skin draining lymph nodes from adult C57BL/6 mice were cultured on plates precoated with anti-mouse α-CD3 (3 μg/ml, Biolegend, #100340) and anti-mouse α-CD28 (3 μg/ml ...
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bioRxiv - Bioengineering 2019Quote: ... MCF-7 cells in EPB were stained with a 1:20 dilution of FITC Anti-human CD326 (Biolegend, Cat.no. 324204) for 30 min at 4 °C to allow for population tracking in flow cytometry ...
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bioRxiv - Cancer Biology 2021Quote: ... For the assessment of CD71 expression in TF-1 and DAMI cells were labeled with the phycoerythrin (PE)-cyanine 7 (Cy7)-conjugated CD71 (BioLegend) (#113811) ...