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Citations for BioLegend :
1301 - 1350 of 7287 citations for 7H Pyrazolo 4 3 d pyrimidin 7 one 1 4 dihydro 3 b D ribofuranosyl oxime monohydrochloride 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 500,000 cells were resuspended in PBS + 1% FBS and spun at 300g for 10 minutes at 4°C and then resuspended in 25 μL staining buffer (BioLegend #420201). 2.5 μL human TruStain FcX (BioLegend #42230 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation for 10 minutes at 20,000 Xg at 4 °C and VASP was subjected to immunoprecipitation using either His6- specific epitope tag affinity matrix (Biolegend #900501) or control antibody matrix (Novagen #69026) ...
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bioRxiv - Bioengineering 2023Quote: ... staining was performed for 30 minutes at 4 C with both 70 nM AlexaFluor647-conjugated HER2 and 1ng/μL anti-DYKDDDDK (anti-FLAG) Tag Antibody-PE (Biolegend, 637310). The 5A12 library was first incubated with either 0.5 nM VEGF (Acro ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed three times (300g, 5 min, 4⁰C) in cell staining buffer and kept in FluoroFix™ Buffer (422101, Biolegend) before run in a BD FACSymphony™ A5 Cell Analyzer (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... cells were washed three times (300g, 5 min, 4⁰C) in cell staining buffer and kept in FluoroFix™ Buffer (422101, Biolegend) prior to run in a BD FACSymphony™ A5 Cell Analyzer (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... cells were co-incubated with human CD16-expressing NK cells (NK92humCD16) for 4 hours at 37°C and the 421™ anti-human CD107a (Biolegend) antibody as a degranulation marker ...
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bioRxiv - Immunology 2024Quote: ... Cultured Raji cells and MV-4-11 cells were washed twice with PBS and stained with FITC-conjugated mouse anti-human CD19 (Biolegend, 302205) for 30 mins ...
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-mouse CD45.1 (Biolegend, cat.: 110735), anti-mouse TER-119 (eBiosciences ...
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bioRxiv - Immunology 2024Quote: ... for 4 h and stained for intracellular expression of either IL-4 or FOXP3 after fixation and permeabilization using the Fixation Buffer (BioLegend, # 420801), the Intracellular Staining Perm Wash Buffer (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... Splenocytes from naive or tumor-bearing AST;Cre-ERT2 mice were mixed with 2×106 C57BL/6 splenocytes and incubated in RPMI-10 for 4 hours at 37°C in the presence of brefeldin A (Biolegend 420601) and TAG peptide (SAINNYAQKL [0.5 μM] ...
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bioRxiv - Biophysics 2021Quote: Flow channels were constructed as previously described but anti-TAMRA antibody was replaced with Anti-ß3 tubulin antibody (BioLegend Poly18020 anti-Tubulin Beta 3). Microtubule nucleation was achieved by introducing 5-50 μM tubulin (1:17 molar ratio of Atto-633 labelled:unlabelled ...
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bioRxiv - Neuroscience 2020Quote: The cerebellar slices were treated either 3 times 24 hours apart with combinations of recombinant TNF (recombinant rat TNF, Biolegend, San Diego, CA, USA), LTα (recombinant human LT-α ...
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bioRxiv - Immunology 2019Quote: Mice were intranasally dosed with 3 µg in 20 µl per mouse recombinant murine TNF-α (r-TNF-α, carrier-free, 575204, BioLegend Ltd, UK) every day for 3 days ...
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bioRxiv - Cell Biology 2022Quote: ... half a million cells per sample were incubated for 10 min in 50 μl of PBS with 3% FSB and 5 μl of blocking buffer (BioLegend, San Diego, CA, USA) and then stained with 50x diluted TotalSeq-B cell feature barcode antibody (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated with PE-streptavidin (BioLegend, 405204), 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Cell Biology 2022Quote: One million fixed BM and spleen cells were immunolabelled for one hour at room temperature with Ter119-PE (1:200, Biolegend) and 1ug/ml Hoechst 33342 (Thermofisher ...
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bioRxiv - Cancer Biology 2021Quote: human HLA-A,B,C-PE (W6/32, Biolegend 311406), FC ...
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bioRxiv - Immunology 2021Quote: ... samples were stained intracellularly with Granzyme B (Pacific Blue, Biolegend) for 30 minutes at room temperature.
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bioRxiv - Microbiology 2022Quote: ... and FITC anti-human/mouse Granzyme B recombinant antibody (Biolegend). Samples were analyzed on the CytoFLEX LX flow cytometer (Beckman Coulter).
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bioRxiv - Immunology 2020Quote: ... B cells were labelled with 7AAD Viability Staining Solution (BioLegend) and 7AAD positive populations were removed ...
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bioRxiv - Immunology 2020Quote: ... and B cells were stained using anti-B220-AF488 (Biolegend). CSF1R-FRed signal was acquired using the 561-diode laser and Csf1r-EGFP or AF488 was acquired using the 488-diode laser both on the FV3000 main combiner (FV31-MCOMB) ...
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bioRxiv - Immunology 2021Quote: ... Alexa Fluor 647 anti-human Granzyme B antibody (GB11, BioLegend), Brilliant Violet (BV ...
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bioRxiv - Physiology 2023Quote: ... B cells were identified as APC-Cy7 B220 (Biolegend, USA); T cells were identified as CD4hi or CD8hi with the following antibodies ...
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bioRxiv - Immunology 2022Quote: ... Treg skew media contained TGF-B (5ng/mL; BioLegend, 763102) and anti-CD28 (2ug/mL ...
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bioRxiv - Immunology 2023Quote: ... granzyme B (clone:QA18A28; PE Biolegend, clone:QA16A02; Alexa Fluor 647 Biolegend), granzyme A (clone:CB9 ...
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bioRxiv - Cancer Biology 2023Quote: ... B cells were identified using APC-Cy7 B220 (Biolegend, USA), while T cells were identified using CD4hi or CD8hi with the following antibodies ...
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bioRxiv - Immunology 2023Quote: ... anti-human HLA-A,B,C (clone W6/32, BioLegend), anti-human CCR7 (clone 3D12 ...
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bioRxiv - Cell Biology 2023Quote: ... and immunoprobed with one of anti-α2-spectrin (Biolegend #803201 ...
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bioRxiv - Cell Biology 2023Quote: ... with one washing step with Cell Staining buffer (BioLegend) between each group of antibodies ...
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bioRxiv - Immunology 2021Quote: ... infarct macrophages as CD45+/CD11b+/Ly6G−/F4/80+ cells and infarct neutrophils as CD45+/CD11b+/F4/80−/Ly6G+ cells.7–9 The following antibodies were used: anti-CD45 (clone 104, 1:100, BioLegend/BD Biosciences); anti-F4/80 (clone BM8 ...
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bioRxiv - Immunology 2020Quote: ... Annexin V/7-AAD staining kit was purchased from Biolegend (#640930).
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bioRxiv - Cancer Biology 2022Quote: ... or 7-AAD Viability Staining Solution (BioLegend, San Diego, CA, USA). The combination of monoclonal antibodies used to identify T-cell subsets is described in the main text ...
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bioRxiv - Immunology 2022Quote: Pacific Blue Annexin V Apoptosis Detection Kit with 7-AAD (BioLegend) was used to characterize cell death by flow cytometry ...
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bioRxiv - Physiology 2021Quote: ... APC Annexin V apoptosis detection kit with 7-AAD (Biolegend, #640930); Sytox™ Blue Dead Cell Stain for flow cytometry (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells were resuspended in sorting buffer with 7-AAD (BioLegend) to gate the viable cells ...
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bioRxiv - Immunology 2023Quote: ... and Phycoerythrin Cyanine 7 (PE-Cy7) mouse anti-human CD11c (BioLegend). The cells were then incubated in the dark on ice for 20 min ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were also incubated with 7-AAD (Biolegend, USA, Catalog #420404) prior to sorting ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: ... 20ng/mL IL-7 and 20 ng/mL IL-33(BioLegend).
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bioRxiv - Cancer Biology 2023Quote: ... PE/Cyanine 7 anti-mouse CD325 (Ep-CAM) antibody (118216, BioLegend); CD31 (PECAM-1 ...
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bioRxiv - Immunology 2024Quote: ... anti-CD4-APC-CY-7 (clone GK1.5) (BioLegend, SanDiego, CA, USA) (1:200) ...
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bioRxiv - Immunology 2024Quote: ... anti-CD107a-PE-Cy-7 (clone 1D4B) (BioLegend, SanDiego, CA, USA) (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... Intracellular P-STAT5 staining was then performed in PBS with wash steps performed at 1800 rpm for 6 min at 4°C.The following antibody clones and fluorochromes were used: CD3 (UCHT1, Biolegend; Bv510, Bv711, FITC), CD8 (SK1 ...
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bioRxiv - Bioengineering 2022Quote: ... For immune cells detections the tumoroids sections also were incubated overnight at 4°C with anti-B220 (Biolegend 103224, APC-cy7 conjugated) and anti-cd11b (Biolegend 101208 ...
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Single cell analysis of the cellular heterogeneity and interactions in the injured mouse spinal cordbioRxiv - Neuroscience 2020Quote: ... supernatant was aspirated and the cells were incubated in blocking buffer mixture for 5min at 4°C (1uL anti-mouse CD16/32 TruStain Fcx (RRID: AB-1877271 AB-1574973, Biolegend Cat #: 101320) and 99uL FACS buffer (1% BSA + 0.05% sodium azide in HBSS w/o Ca2+/Mg2+)) ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were collected by centrifugation (1500rpm; 5mins; 4°C) and washed twice with Annexin V binding buffer (Biolegend; 1ml per wash). The cell pellets were resuspended in of binding buffer (100μl ...
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bioRxiv - Immunology 2020Quote: ... Unbound antibodies were removed by washing with PBS were added at 5 × 104 cells/well in the presence of 4 μg/mL brefeldin A (Biolegend, Cat # 420601), 5 μg/mL GolgiStop (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Then these PBMCs was incubated with mixed antibodies cocktail at 4 °C for 30 min (the antibodies cocktail including FITC-anti-human CD19 antibody (Biolegend, clone: SJ25C1), BV421-anti-human IgD antibody (Biolegend ...
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bioRxiv - Genetics 2021Quote: ... The cells were then incubated for 20 min at 4°C with the TotalSeq-C antibody mix: TotalSeq - C0048 anti-human CD45 Antibody (Biolegend; Cat# 368545), TotalSeq - C0103 anti-mouse/human CD45R/B220 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... cytokine staining mixture (containing anti-IFN-γ, anti-IL-2, anti-IL-4, anti-IL-10 and anti-TNF-α antibodies, Biolegend) was added to each well ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed and stained in PermWash buffer (eBioscience) for 30 min at 4°C with Abs (purchased from BioLegend unless noted) against Bcl2 (BCL/10C4) ...