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Citations for BioLegend :
901 - 950 of 7287 citations for 7H Pyrazolo 4 3 d pyrimidin 7 one 1 4 dihydro 3 b D ribofuranosyl oxime monohydrochloride 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and stained (30 min at 4°C) with PE anti-TLR4 (Biolegend; clone Sa15-21; 145404) in blocking buffer (PBS with 2% rat serum) ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed 4 times with PBS and 40μl of HRP anti-rabbit IgG antibody (Biolegend) 1:1500 was added to each well and incubated for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... for one hour at room temperature followed by APC-Streptavidin secondary (1:500, Biolegend, 405207) and nuclear DAPI (0.1 μg/mL ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells (1 × 106) were stimulated for 7 days at 37°C with anti-mouse CD3 (BioLegend; 1 μg/mL) and CD28 (BioLegend ...
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bioRxiv - Physiology 2019Quote: ... and FITC conjugated antibodies against B220 to identify B cells (1:100, catalog number 103206, BioLegend). Secondary antibodies conjugated to AlexaFluor 405 (1:400 ...
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bioRxiv - Microbiology 2022Quote: ... GC B cells stained using rat anti-GL7 antibody (FITC; BioLegend, catalog no. 144604, 1:250), Tfh cells stained using anti-CD4 antibody (BioLegend ...
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bioRxiv - Pathology 2022Quote: ... and cells were stained in FACS buffer for 30 min at 4°C with the following anti-mouse antibodies: PacificBlue-conjugated anti-CD45 (Biolegend, clone 30-F11, #103126, 1/100), PE-Cy7-conjugated anti-CD11b (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... and PeCy-7-CD8 (BioLegend Cat# 100722). IFN-γ- and IL-17A- producing T cells were determined by intracellular cytokine assays as previously described (19 ...
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bioRxiv - Immunology 2019Quote: ... CD38-BV650 (clone HB-7) (all Biolegend), CD27-biotin (clone O323 ...
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bioRxiv - Genomics 2019Quote: ... BV785 anti-CD38 (Biolegend, Clone: HB-7), BV650 anti-CD19(Biolegend ...
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bioRxiv - Bioengineering 2020Quote: ... CD68 (PE/Cyanine 7, BioLegend catalog# 137015), and F4/80 (APC/Cyanine 7 ...
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bioRxiv - Cancer Biology 2022Quote: ... Viability was evaluated by 7-AAD (Biolegend) staining that was added to samples 5 min before sorting ...
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bioRxiv - Immunology 2019Quote: ... and IL-7 (2 ng/mL, Biolegend) at 37°C for 2 days ...
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bioRxiv - Immunology 2020Quote: ... PerCP-Cy5.5 anti-CD38 (HB-7, Biolegend), Brilliant Violet 421 anti-CD27 (M-T271 ...
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bioRxiv - Immunology 2020Quote: ... CD49a (TS2/7, Alexa Fluor 647, Biolegend), CD45 (HI30 ...
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bioRxiv - Immunology 2020Quote: ... CD38-PE-Cy7 (clone HB-7; BioLegend), CD27-Pacific Blue™ (clone O323 ...
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bioRxiv - Microbiology 2020Quote: ... and 7-AAD viability staining solution (BioLegend) by 1:200 ...
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bioRxiv - Immunology 2022Quote: ... anti-CD51 PE (Biolegend, clone RMV-7), anti-CD31 PE-Cy7 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... CD38 (PE-Cy7 clone HB-7, Biolegend), IgD (FITC polyclonal ...
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bioRxiv - Immunology 2023Quote: ... anti-Thy1.1 (OX-7) all from Biolegend; anti-CD103 (2E7) ...
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bioRxiv - Immunology 2023Quote: ... CD11a-PE/Cy7 (I21/7, Biolegend 153108), CD62L-APC/Cy7 (MEL-14 ...
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bioRxiv - Immunology 2024Quote: ... and IL-7 (20 ng/ml, Biolegend) for 2 days ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lines or expanded primary NK cells were stimulated with 3 ug/mL CD28 activating antibody (Biolegend, cat#302933) for 24 hours.
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bioRxiv - Immunology 2021Quote: ... spleen T cell IL-1β/IL-23 plus spermidine treatment group (SPT sper) with hashtag 3 (BioLegend, Cat# 155805); lymph node cell IL-1β/IL-23 stimulation group (LN stim ...
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bioRxiv - Physiology 2022Quote: ... Cells were then washed in HBSS with 3% BSA and incubated with CD34 Brilliant Violet 421 (BioLegend 230 119321) at 1:400 and CD24 PerCP-Cyanine 5.5 (eBioscience ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were washed three times in FACS buffer and resuspended in FACS buffer containing 3 μg/ml DAPI (BioLegend). Flow cytometric analysis was done on a BD LSRFortessa analyzer and sorting was done on a BD FACSAria1 machine.
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bioRxiv - Genomics 2021Quote: ... for RNA library construction while modifications for CITE-seq ADT and cell hashing HTO library preparation were performed according to the TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 or v3.1 single index protocol (BioLegend). The modifications include the addition of ADT and HTO additive primers to a final concentration of 0.2 uM in the cDNA amplification reaction (step 2.2) ...
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bioRxiv - Immunology 2022Quote: ... added for last 3 hrs of stimulation and stained with IFN-γ (XMG1.2; eBioscience) and IL-17A (TC11-18H10.1; Biolegend) using a Cytofix/Cytoperm kit (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... These cells were stimulated with 10μg/ml of anti-CD3 (OKT-3) and anti-CD28 (28.2) antibodies (Ultra-LEAF™, Biolegend) in R10-50-15 for 48 hours ...
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bioRxiv - Bioengineering 2020Quote: ... dilutions of the serum were mixed with a constant 3 nM of an HRP conjugated anti-OVA antibody (BioLegend) and incubated for 2 h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... mice were injected into the retro-orbital sinus with a total of 3 mg of PE/Cy7-CD45.2 (104, BioLegend) in 200 μl sterile DPBS ...
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bioRxiv - Immunology 2023Quote: ... 130-120-662) for 3 days in 24-well plates coated with 3μg/ml anti-CD3 (145-2c11; Biolegend) and 3μg/ml anti-CD28 (37.51 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... For the HashTag library TotalSeq™-B0303 anti-mouse Hashtag 3 Antibody (Clone M1/42; 30-F11; 155835; BioLegend) was used to mark single cells of DMSO treated mice and TotalSeq™-B0304 anti-mouse Hashtag 4 Antibody (Clone M1/42 ...
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bioRxiv - Immunology 2024Quote: ... intestine and skin (n = 3, PCW 9.5) were stained with the following antibodies (clone, fluorophore, company): CD3 (UCHT1, FITC, Biolegend), CD7 (M-T701 ...
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bioRxiv - Synthetic Biology 2024Quote: ... 50,000 CAR-T cells were co-cultured with 100,000 target cells for 5h in the presence of CD107a antibody (Supp. Table 3) and 1x Brefeldin A (Biolegend). Following co-culture ...
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bioRxiv - Immunology 2024Quote: ... which is RPMI-1640-based and supplemented with 5 μg/mL recombinant mouse IL-3 (#575506 or #575508, BioLegend) as described in previous studies (6 ...
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bioRxiv - Immunology 2024Quote: Single cell suspensions from 3 B16-OVA WT tumours were labelled with TotalSeq(TM)-C0301 antibody (Biolegend, Cat. 155861), and 3 IFNγRKO tumours were labelled with TotalSeq(TM)-C0302 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... coupled with the protocol TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 or v3.1 (Single Index) from BioLegend.
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bioRxiv - Cell Biology 2021Quote: ... 0.1% Sodium Azide and with one of the following primary antibody dilutions: Mouse Anti-HA (1:2000; HA.11, Biolegend), Mouse Anti-V5 (1:2000 ...
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bioRxiv - Cell Biology 2022Quote: ... Stained cells and “fluorescence minus one” controls were then resuspended in SB + 1:1000 DNase + 1:1000 Draq7 (Biolegend, #424001) as a live/dead stain ...
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bioRxiv - Immunology 2023Quote: ... Each sample was labelled with one specific hashtag (1:400 dilution) and CITE-seq antibodies (1:800 dilution, all Biolegend). After labelling for 30 min at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... Synomag-D+ DC were intradermally injected into each hind footpad immediately following Tag-It Violet™ (Violet+) cell tracking dye incorporation (Biolegend, San Diego, USA). MPI was done immediately following injection (day 0 ...
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bioRxiv - Immunology 2023Quote: Plasma and culture supernatants were collected and frozen at -80°C until cytokines measurement by commercial ELISA kits (human IL-1ß and IL-18 R&D Systems; human TNF-α and IFN-γ, Biolegend).
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bioRxiv - Immunology 2021Quote: Splenic B cells were isolated from bone marrow chimeras (MojoSort™ Mouse Pan B Cell Isolation Kit; Biolegend). Control and WNK1-deficient B cells were labelled with CellTracker blue (CMAC ...
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bioRxiv - Microbiology 2023Quote: Naïve B cells were isolated from spleens by immunomagnetic depletion using MojoSort Pan B cell Isolation Kit (Biolegend) and cultured in RPMI supplemented with L-glutamine ...
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bioRxiv - Cell Biology 2019Quote: ... cells were then incubated for 10 minutes at 4°C in human Fc Blocking reagent (FcX, BioLegend). Cells were incubated at 4°C for a further 30 minutes with 0.5µg of TotalSeq™-A (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... lymph node cell IL-1β/IL-23 stimulation group (LN stim) with hashtag 4 (BioLegend, Cat# 155807); lymph node cell IL-1β/IL-23 plus spermidine treatment group (LN sper ...
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bioRxiv - Immunology 2021Quote: ... and cells were instead stained with 4′,6-diamidino-2-phenylindole (DAPI; BioLegend, San Diego, CA, USA) and gated for live (DAPI- ...
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bioRxiv - Genetics 2019Quote: ... anti-CD25-PE (8 µl) and anti-CD45RA-BV785 (4 µl, clone HI100, Cat. no. 304140, BioLegend), incubated at RT in the dark for at least 30 min. ...
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bioRxiv - Cell Biology 2021Quote: ... cells were then incubated for 10 minutes at 4°C in human Fc Blocking reagent (FcX, BioLegend). Cells were incubated at 4°C for a further 30 minutes with 0.5μg of TotalSeq-A (Biolegend ...