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Citations for BioLegend :
1001 - 1050 of 7287 citations for 7H Pyrazolo 4 3 d pyrimidin 7 one 1 4 dihydro 3 b D ribofuranosyl oxime monohydrochloride 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Cocktail 1 was used to identify CD3+CD14+ cells and contained 3 μl of anti-human CD3-AF700 antibody (clone UCHT1, Biolegend) and 1 μl of anti-human CD14-PE antibody (clone 61D3 ...
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bioRxiv - Developmental Biology 2020Quote: Cells were resuspended in 5ml CMF-PBS with 3% BSA (MilliporeSigma, A9576-50ml) and plated on anti-GD3 (Biolegend, 917701) 10cm plates (untreated plates coated overnight at 4°C with 13μg GD3 antibody in 10ml 50mM Tris-HCl pH9.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... for 3 min and stained using mouse anti-human HER2 antibody conjugated to FITC (20 μg/ml, clone 24D2, BioLegend). Cells were incubated with anti-HER2 antibody for 20 min at 4°C in the dark and washed once with PBS supplemented with 1% FCS prior to the FACS analysis ...
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bioRxiv - Immunology 2020Quote: ... mice were anesthetized with Ketamine/Xylazine (Patterson Veterinary) and injected intravenously with 3 μg of fluorophore-labelled anti-CD45 (30-F11, Biolegend) monoclonal antibody through the retro-orbital sinus to label intravascular leukocytes ...
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bioRxiv - Genomics 2020Quote: Cell and nuclei labelling were performed according to an adapted Biolegend cell hashing protocol (TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 3.1 Protocol, Biolegend). Briefly ...
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bioRxiv - Genomics 2021Quote: ... The MNC fraction was depleted of red blood cells by lysis steps involving 3 incubations at room temperature for 20 mins/10 mins/10 mins respectively with RBC lysis buffer (BioLegend). Tissue lymphocytes were isolated from Peyer’s patches in intestinal mucosa using laser capture microdissection and subjected to protein lysis as outlined above ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell viability was assessed by flow cytometry after 3 days of drug or vehicle treatment using Helix NP NIR viability dye (BioLegend). Dose-response curves were fitted through interpolation using GraphPad Prism v.7 nonlinear regression fit ([inhibitor] versus normalized response–variable slope).
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bioRxiv - Synthetic Biology 2021Quote: ... incubated at a concentration of 0.1 µg/mL in HF for 3 minutes at room temperature or Zombie NIRTM (BioLegend, 423106) incubated in PBS at a 1:500 dilution for 15 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: 3×106 cells from each tonsil donor (n=3) at each time point were stained with the Zombie Red fixable viability kit (Biolegend) for 10 min at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... protein expression (ADT) and hashtag (HTO) libraries were generated using Chromium Single Cell 3’ version 2 reagents (10x genomics and Biolegend) per manufacturers’ protocols ...
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bioRxiv - Immunology 2022Quote: ... resuspended in 50 μl FACS-buffer containing the respective fluorochrome-coupled Abs (anti-TACI/CD267 PE, clone eBio8F10-3, eBioscience, anti CD138 PECy7, clone 281-2, Biolegend, anti CD19 BV510 ...
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bioRxiv - Immunology 2023Quote: ... Panels 2 and 3 incorporated anti-Digoxygenin (RD System, #611621), anti-FITC (Standard BioTools, #3174006B) and anti-Biotin (Biolegend, #409002) metal-tagged antibodies to detect the RNAscope staining output.
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bioRxiv - Immunology 2023Quote: ... Pooled cells from n = 3 - 5 mice/cohort were stained with PE- or APC-conjugated antibodies (from either BD or BioLegend) against murine F4/80 (clone ...
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bioRxiv - Immunology 2023Quote: ... Cells were activated for 3 h in a round-bottom 96-well plate using Cell Activation Cocktail (with Brefeldin A) (BioLegend). Plates were then centrifuged at 300 x g for 5 min and the pellets resuspended in 50 µL of Cell-ID™ Cisplatin-195Pt viability reagent (Standard BioTools) ...
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bioRxiv - Immunology 2023Quote: ... THP-1 and SKOV-3 cell lines and primary human T cells were labeled with fluorescent antibody against human CD70 or isotype control (Biolegend) to verify antigen expression as described before35.
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bioRxiv - Immunology 2023Quote: BMMCs were generated from the BM cells of C57BL/6 mice (Japan SLC, Hamamatsu, Japan) by cultivation in the presence of 5 ng/mL of mouse IL-3 (BioLegend) as previously described 18 ...
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bioRxiv - Genomics 2023Quote: ... Fluorophore conjugated antibodies against the following antigens were used for input and day 3 cells (All Biolegend unless otherwise noted): panel 1 ...
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bioRxiv - Immunology 2023Quote: ... 106 of the CTV labeled T cells were stimulated in vitro for 3 or 5 days in cultures containing 100ng/ml IL-15 (BioLegend) or a combination of 100ng/ml IL-7 (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... Viability of BMNC (0.25 x106) was assessed by Annexin V and 7-AAD staining (APC Annexin V Apoptosis Detection Kit 7-AAD, Biolegend) using FCM.
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bioRxiv - Microbiology 2022Quote: ... total lymphocytes were counted and naïve B cells were isolated using Mojosort Naïve B cell isolation beads (Biolegend 480068) or Naïve B cell Isolation Kit II (Miltenyi 130-091-150 ...
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bioRxiv - Immunology 2019Quote: ... anti-CD38– APC-Cy7 (clone HB-7; BioLegend), and anti-CD20–FITC (clone 2H7 ...
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bioRxiv - Cell Biology 2021Quote: ... surface staining was performed using 7-AAD (Biolegend) and fluorochrome-tagged antibodies to CD45 and CD117 (both from Biolegend) ...
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bioRxiv - Cancer Biology 2022Quote: ... CD45 PE Cyanine 7 (BioLegend Cat# 304125, RRID:AB_10709440), CD42b APC Cyanine7 (BioLegend Cat# 303920 ...
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bioRxiv - Immunology 2022Quote: ... anti-CD49a PE/Cy7 (clone TS2/7, Biolegend), anti-CD45 allophycocyanin (APC ...
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bioRxiv - Immunology 2022Quote: ... Dead cells were stained with 7-AAD (Biolegend) or ZOMBIE NIR (Biolegend) ...
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bioRxiv - Immunology 2020Quote: ... anti-GL-7-PE-Cy7 (cat.no:144620,Biolegend),anti-IgD-BV786 (cat.no ...
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bioRxiv - Cancer Biology 2020Quote: ... the cells were stained with 7-AAD (Biolegend) for live/dead discrimination ...
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bioRxiv - Immunology 2019Quote: ... and CD56-Phycoerythrin Cyanine 7 (clone NCAM; BioLegend).
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bioRxiv - Microbiology 2019Quote: ... Cellular DNA was counterstained with 7-AAD (BioLegend) and the cells were analyzed on a BD LSR Fortessa instrument.
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bioRxiv - Immunology 2020Quote: ... 7-AAD viability staining solution was from Biolegend. MART-1 peptide (MART-126-35A27L ...
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bioRxiv - Systems Biology 2019Quote: ... trypsinized and stained with 7-AAD (BioLegend 420404) or Zoombie NIR (BioLegend 423105 ...
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bioRxiv - Immunology 2019Quote: ... anti-αV integrin RMV-7 was from BioLegend, and anti-Mac1 mAb M1/70 was purified from hybridoma supernatant ...
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bioRxiv - Immunology 2022Quote: ... Dead cells were excluded using 7-AAD (Biolegend).
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bioRxiv - Immunology 2022Quote: ... anti-CD49a PE/Cy7 (clone TS2/7, Biolegend), anti-CD45 allophycocyanin (APC ...
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bioRxiv - Systems Biology 2023Quote: ... Cell viability was determined using 7-AAD (BioLegend) or PI staining solution (Invitrogen eBioscience) ...
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bioRxiv - Cell Biology 2023Quote: ... cell suspensions were spiked with 7-AAD (BioLegend) to label non-viable cells ...
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bioRxiv - Immunology 2023Quote: ... cells were stained with 7-AAD (BioLegend, #420404) cellular DNA content marker (5 μl per sample ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated at 4°C for a further 30 minutes with 0.5µg of TotalSeq™-A (Biolegend) monoclonal anti-CD10 ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained with the following antibodies at 4°C for 45 minutes: CD56-BV421 (HCD56, BioLegend), CD4-BV605 (OKT4 ...
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bioRxiv - Immunology 2021Quote: ... for 1 hour and subsequently stained overnight at 4°C with mouse anti-human TCR Vα7.2-PE (Clone 3C10, Biolegend), mouse anti-human CD161-AF488 (Clone NKR-P1A ...
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bioRxiv - Immunology 2019Quote: ... The reconstitution efficiency was analyzed by FACS 4 weeks after BMT using CD45.1-APC (A20; eBioscience) and CD45.2-PerCP (104; Biolegend) antibody.
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bioRxiv - Neuroscience 2021Quote: ... single cell suspensions were incubated for 15 minutes at 4°C in Zombie NIR Fixable viability dye (Biolegend) diluted 1:500 in PBS ...
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bioRxiv - Immunology 2021Quote: ... for 15 minutes on ice followed by staining with fluorescently conjugated Abs: anti-CD11a (clone M17/4- Biolegend), anti-KLRG1 (clone MAFA- Biolegend) ...
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bioRxiv - Microbiology 2021Quote: ... primary CD4+T cells were activated for 4 days with 1μg/ml plate-bound αCD3 antibody (cloneOKT3, Biolegend) in the presence of 2 μg/mL soluble αCD28 antibody(clone CD28.2 ...
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bioRxiv - Immunology 2022Quote: ... and then stained for 30 min at 4°C with a FITC-conjugated anti-mouse CD4 Ab (BioLegend) and PE-conjugated anti-mouse CCR5 (a Th1 marker (27)) ...
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bioRxiv - Immunology 2022Quote: ... and then stained for 30 min at 4°C with a FITC-conjugated anti-mouse CD4 Ab (BioLegend) before fixation ...
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bioRxiv - Immunology 2022Quote: ... and then stained for 30 min at 4°C with a FITC-conjugated anti-mouse CD4 Ab (BioLegend) and PE-conjugated anti-mouse CCR5 (a Th1 marker (27)) ...
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bioRxiv - Immunology 2022Quote: ... BioLegend) for 30min at 4 °C and intracellular markers (BV421-labeled anti-mouse IFN-γ, Cat# 505830, BioLegend; FITC-labeled anti-mouse IL-2 ...
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bioRxiv - Microbiology 2022Quote: ... and stained intracellularly for 30 minutes in PBS with antibodies including: PE anti-mouse IL-4 antibody (Biolegend) or PE anti-mouse IFN-γ antibody (Biolegend) ...
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bioRxiv - Developmental Biology 2020Quote: Freshly isolated mammary cells were incubated at 4°C for 20 min with the following antibodies from Biolegend: anti-CD24-BViolet421 (clone M1/69 ...