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Citations for BioLegend :
851 - 900 of 1319 citations for 3 2 Methylphenoxy methyl piperidine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... IFNg-PeCy7 (cat # 505826) and IL-2-PB (cat # 503820) were purchased from Biolegend. CD44-Percp-cyanine5.5 (cat# 45-0441-80 ...
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bioRxiv - Immunology 2023Quote: ... Cells were isolated from blood by adding 2 mL of RBC lysis buffer (Biolegend) per 100 uL of blood collected ...
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bioRxiv - Immunology 2024Quote: IL-2 and IFN-γ levels were measured with a sandwich ELISA kit (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... or SARS-CoV-2 nucleocapsid peptide pool together with 5μg/ml Brefeldin A (Biolegend) for 16 hours ...
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bioRxiv - Immunology 2022Quote: ... media was changed to complete EGM-2 with 1 ng/mL TNFα (Biolegend 575206) and shear stress of 1 dyn/cm2 was incorporated using a microfluidic pump (Ibidi 10902 ...
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bioRxiv - Synthetic Biology 2022Quote: ... and IL-2 or IFNγ were measured by ELISA following the manufacturer’s protocol (BioLegend). Absorbances were measured with a Synergy H1 Hybrid Multi-Mode Reader (BioTek).
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bioRxiv - Cell Biology 2022Quote: ... Phycoerythrin-conjugated anti-human tetherin (BioLegend, RS38E, FC 2 μg / 100 μl / 106 cells), Pacific Blue-conjugated anti-CD71 (Exbio ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1-2 million cells were incubated in 45ul of Cell Staining Buffer (BioLegend) per million cells with Trustain FcX block (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... BV421 rat anti-mouse CD45 (cat # 103133, BioLegend, clone 30-F11, 2 ng/μl), PE rat anti-mouse CD11b (cat # 101208 ...
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bioRxiv - Physiology 2024Quote: ... with 2% with Fetal Bovine serum with Fc block (Biolegend, San Diego, California, USA) and stained with monoclonal antibodies specific for CD66b (clone GM2H6) ...
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bioRxiv - Cancer Biology 2024Quote: Experiment 2: All conditions were multiplexed using Hashtag Oligonucleotide barcoding technology (TotalSeq-B, Biolegend) following manufacturer standard protocol ...
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bioRxiv - Immunology 2024Quote: ... Leukocytes were blocked with 2% normal mouse serum and 1% FcX Block (BioLegend, #101320) in FACS buffer for 30 minutes at 4°C prior to being stained with Zombie NIR (Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-I-A/I-E-Bv650 (cat#107641, Clone M5/114/15/2, Biolegend), anti-CD103-PE/Cyanine7 (cat#121425 ...
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bioRxiv - Cell Biology 2024Quote: ... resuspended in complete media supplemented with 20 ng/mL rm-IL-2 (Biolegend, #575406), and cultured in a T75 flask at 5×105 cells/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... VDR overexpressing SKOV-3 and ECC-1 cells were processed and stained with PDL1-BV605 antibody (Biolegend Inc, Catalog number:124321). VDR expressing SKOV-3 and ECC-1 cells ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL solution were then stained with 3 μL FITC anti-human CD20 antibody (BioLegend, 302304), 3.5 μL Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
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bioRxiv - Microbiology 2021Quote: ... Tissues were incubated with primary antibodies (mouse anti-HuC/D IgG clone 16A11 at 1:200 [Thermofisher], rabbit polyclonal anti-tubulin β-3 IgG at 1:500 [Biolegend]) in PBS containing 1% Triton X-100 for 48 h at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Every 106 B cells in 100 μl solution were stained with 3 μl FITC antihuman CD20 antibody (BioLegend, 302304, clone: 2H7), 3.5 μl Brilliant Violet 421 antihuman CD27 antibody (BioLegend ...
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bioRxiv - Molecular Biology 2020Quote: ... Linker oligo with 3’-terminal biotin was purchased from IDT (Coralville, Iowa) and purified streptavidin was purchased from BioLegend (Biolegend, #405150). Biotinylated antibody ...
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bioRxiv - Cell Biology 2021Quote: ... T cells were isolated and stimulated for 48 h with 3 μg/ml plate-bound anti-mouse CD3 antibody (100340, BioLegend, USA) and 2 μg/ml soluble anti-mouse CD28 antibody (102116 ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were blocked with PBS containing 3% BSA and stained with either APC-conjugate anti-human CD99 antibody clone 3B2/TA8 (1:20 dilution, BioLegend 341307), PE anti-human CD166 clone 3A6 (1:20 dilution ...
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bioRxiv - Microbiology 2021Quote: ... Sections were blocked with 10% sheep serum and 1% BSA in TBS for 30 min then incubated at 4°C overnight with 1 μg ml−1 rabbit polyclonal anti-tubulin β-3 IgG (Biolegend) and 1% BSA in TBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Wells were washed 3 times before adding 50 µL per well of Biotin anti-human IFN-γ Antibody (clone 4S.B3; #502504, BioLegend, USA) diluted 1:400 in 1% SureBlock solution and incubated 1 h at RT ...
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bioRxiv - Genetics 2023Quote: ... Recombinant Anti-AKT1 + AKT2 + AKT3 (phospho S472 + S473 + S474, Purified anti-Tubulin β-3 (TUBB3) Antibody 1:1000 (Biolegend, # PRB-435P), anti-GAPDH antibody 1:1000 (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: Oligo-labeled antibody staining was done on both melanoblasts and melanom cells using the TotalSeq™-A Antibodies and Cell Hashing with 10× Single Cell 3′ Reagent Kit (Biolegend). The HashTag Oligonucleotidess used for the analysis are mHTO01 ...
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bioRxiv - Cancer Biology 2022Quote: ... spun at 700 g for 3 minutes at 4°C and washed in MACS Buffer 3× before being incubated with the following antibodies: APC-conjugated rat anti-CD3 (5 μL/10×106 cells; Biolegend, 100408), PE-conjugated rat anti-CD8b (2.5 μL/10×106 cells ...
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bioRxiv - Bioengineering 2024Quote: ... hiPSC-CMs were dissociated by enzymatic digestion (Miltenyi Multi Tissue dissociation kit 3) and stained with the Zombie NIR™ Fixable Viability Kit (BioLegend). Then ...
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bioRxiv - Immunology 2023Quote: To determine galectin-3 or galectin-8 expression the following: intracellular protein and lysosomes were stained using anti-Lamp1-BV421 (clone H4A3, BioLegend, USA), anti-Galectin-3-AF488 (clone B2C10 ...
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bioRxiv - Immunology 2023Quote: ... 1x106 B cells in 100 μl buffer were incubated with a panel of antibodies including 3 μl FITC anti-human CD20 antibody (BioLegend, 302304), 3.5 μl Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Cells were rinsed with FACS buffer (PBS containing 0.5% BSA and 2mM EDTA) for 3 min at 300g and incubated with Fc Receptor Blocking solution (Human TruStain FcX™, Biolegend) for 10 minutes prior to cell surface staining with fluorescently labeled antibodies in Brilliant Stain buffer (BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... and preMG were resuspended in 3:1-medium supplemented with 50 ng/mL recombinant human MCSF (Macrophage Colony Stimulating Factor, BioLegend, #574804). Cells were counted using an automated cell counter (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... 0.3% triton in PBS) for 3 hours then incubated with Alexa Fluor 594 anti-β-amyloid (Clone 6E10; 1:1,000; BioLegend; 803019), Olig2 (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... diluted in PBS with NHS and Triton X (both at 1%): 1) mouse isotype IgG2a anti-Tubulin beta 3 (TUJ1, 1:1000, BioLegend #801201), 2 ...
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bioRxiv - Cell Biology 2024Quote: ... The cell suspension was distributed over a 96-well plate (200 μL/well) that had been precoated with 3 μg/mL anti-CD3ε (Biolegend, #1000340) for 2 h ...
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bioRxiv - Cancer Biology 2021Quote: ... and 2 mM EDTA then stained with anti-mouse CD31-APC (1:500, Biolegend, 102507), CD45-APC (1:500 ...
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bioRxiv - Immunology 2021Quote: ... at 1:100 dilution in 2% BSA-PBS prior to using streptavidin BV421 (405225, BioLegend) and anti-human VRC01 mAb conjugated to AF488.
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bioRxiv - Microbiology 2020Quote: ... PBS supplemented with 0.1 µg/ml antifade-4 6-diamidino-2-phenylindole (DAPI) (BioLegend, USA) was added to the cells for at least 30 min before imaging ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... resuspended in 1x DAPI solution (in PBS plus 0.5% BSA and 2 mM EDTA; BioLegend) at 5×106 cells/ml ...
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bioRxiv - Immunology 2021Quote: ... Cells were subsequently blocked with 2 μL of TruStain FcX Fc receptor blocking antibody (BioLegend) for 10 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... 2×106 cells were incubated for 5 hours with Brefeldin A (BioLegend, San Diego, California) and Monensin (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... Lung cells were plated at 2 million cells/well and incubated with Zombie UV (Biolegend) for dead cell discrimination ...
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bioRxiv - Neuroscience 2021Quote: ... and Alexa Fluor 647 anti-CD106 antibody (2 μg/mL, VCAM-1, 429 MVCAM.A, BioLegend) were used ...
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bioRxiv - Immunology 2023Quote: ... The 96-well ELISA plates were pre-coated with purified anti-mouse IL-2 (BioLegend) using coating buffer (0.1 M Na2HPO ...
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bioRxiv - Immunology 2023Quote: ... hydrogel beads were stained with anti-CD138 PE (1:80 dilution, clone 281-2, BioLegend), anti-FLAG PE-Cy7 (1:200 dilution ...
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bioRxiv - Microbiology 2023Quote: ... we administered 2 μg of APC/Fire750-labeled anti-CD45 mAb (Biolegend, clone 30-F11) in mice anesthetized with an overdose of ketamine and xylazine ...
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bioRxiv - Biophysics 2023Quote: ... and 6.25 μg/mL activation antibodies against Integrin β2 (clone m24 and LFA1/2, Biolegend) were added in cell culture medium at the start of the culture.
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bioRxiv - Bioengineering 2024Quote: ... Each tube of cells then received 2 µL of Fc block (BioLegend, San Diego, CA), 10 µL of Brilliant Stain Buffer (Becton Dickinson) ...
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bioRxiv - Genomics 2023Quote: ... cells were incubated in the antibody mix (2 μg/ml rat anti-CD326 (BioLegend 312502), 1 μg/ml rabbit anti-RAD51-AF647 (Bioacademia 70-012) ...
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bioRxiv - Immunology 2024Quote: ... The cells were then stained with a cocktail of cell surface antibodies (Biolegend, Table 2) in 0.5%BSA/PBS and analyzed on a LSR II flow cytometer (BD Biosciences) ...
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bioRxiv - Biochemistry 2023Quote: ... Western blot analysis of the samples were done using anti- HA (1:2 000; BioLegend) and anti-Flag M2 (1:5 000 ...