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Citations for BioLegend :
801 - 850 of 1319 citations for 3 2 Methylphenoxy methyl piperidine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... rabbit) at 1:100 dilution (for immunodepletion) or 3 μg/ml of each 4G8 and 6E10 anti-Aβ antibodies (Biolegend) (for brain-derived Aβ enrichment ...
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bioRxiv - Microbiology 2023Quote: ... for 5 minutes at 4°C and then fixed using commercial fixation buffer for 3 minutes (ref no. 420801, BioLegend). For intracellular staining of Granzyme B and Perforin ...
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bioRxiv - Immunology 2023Quote: ... Sip-T or single cell suspensions of tumor-infiltrating lymphocytes (TILs) were resuspended in PBS as 1-3×106 cells/ml and stained with fixable Zombie Aqua viability dye (BioLegend) as per manufacturer’s recommendation ...
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bioRxiv - Immunology 2023Quote: ... Pooled cells from n = 3 - 5 mice/cohort were stained with PE- or APC-conjugated antibodies (from either BD or BioLegend) against murine F4/80 (clone ...
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bioRxiv - Immunology 2023Quote: ... Cells were activated for 3 h in a round-bottom 96-well plate using Cell Activation Cocktail (with Brefeldin A) (BioLegend). Plates were then centrifuged at 300 x g for 5 min and the pellets resuspended in 50 µL of Cell-ID™ Cisplatin-195Pt viability reagent (Standard BioTools) ...
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bioRxiv - Immunology 2023Quote: ... 106 of the CTV labeled T cells were stimulated in vitro for 3 or 5 days in cultures containing 100ng/ml IL-15 (BioLegend) or a combination of 100ng/ml IL-7 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... naïve CD8+ T cells were cultured in RPMI (10% FCS) and stimulated with plate-bound mCD3 mAbs (3 µg ml-1, clone 145-2C11, Biolegend), soluble mCD28 (0.5 µg ml-1 ...
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bioRxiv - Immunology 2022Quote: 3×106 cells from each tonsil donor (n=3) at each time point were stained with the Zombie Red fixable viability kit (Biolegend) for 10 min at RT ...
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bioRxiv - Immunology 2023Quote: ... THP-1 and SKOV-3 cell lines and primary human T cells were labeled with fluorescent antibody against human CD70 or isotype control (Biolegend) to verify antigen expression as described before35.
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bioRxiv - Cell Biology 2023Quote: ... samples were stained for proliferation marker Ki-67 Alexa Fluor 647 (1:250 dilution in 3% BSA, Cat. No. 652407; BioLegend) for 1 hour and nuclei counterstain bisbenzimide (1:1000 dilution in 1X dPBS ...
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bioRxiv - Cell Biology 2023Quote: ... Single cells were pelleted and re-suspended in a staining solution of DMEM containing 1% FBS and anti-Ki-67 Alex Fluor 647 (1:250 dilution in 3% BSA, Cat. No. 652407; BioLegend) for 1 hour on ice ...
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bioRxiv - Immunology 2023Quote: BMMCs were generated from the BM cells of C57BL/6 mice (Japan SLC, Hamamatsu, Japan) by cultivation in the presence of 5 ng/mL of mouse IL-3 (BioLegend) as previously described 18 ...
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bioRxiv - Genomics 2023Quote: ... Fluorophore conjugated antibodies against the following antigens were used for input and day 3 cells (All Biolegend unless otherwise noted): panel 1 ...
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bioRxiv - Cell Biology 2024Quote: ... the sperm smears were fixed for 10 min with 3.2 % paraformaldehyde and permeabilized with the Intracellular Staining Perm Wash Buffer for 3 x 5 min (421002, BioLegend, USA). Following permeabilization ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were fixed with cold methanol for 3 min at room temperature and incubated overnight at 4 °C with an anti-HA antibody (diluted 1:2000) (Biolegend,). Cells were then rinsed three times with PBS and incubated for 1 h at room temperature with an Alexa647-conjugated goat anti-mouse antibody (diluted 1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Residual red blood cells (RBC) were lysed incubating cell suspension for 3 minutes at 4°C with 1X RBC Lysis Buffer (BioLegend). Cell suspensions were washed with PBS ...
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bioRxiv - Immunology 2024Quote: ... Staining was performed by washing with 0.1% Triton-X 100 in PBS for 3 x 15 minutes then staining for CD3-PE (1:100, Biolegend, 100205) overnight at room temp ...
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bioRxiv - Immunology 2024Quote: ... bone marrow progenitor cells were harvested from the femur of C57BL/6 mice and plated at a density of 3×106/3mL well on 6-well plates with 20 ng/mL GM-CSF (BioLegend) in RPMI 1640 medium (Wisent ...
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bioRxiv - Immunology 2024Quote: ... bone marrow cells were plated at 3×105/mL density in IMDM medium containing 20 ng/mL mouse GM-CSF (BioLegend) and cultured for 7-9 days.
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bioRxiv - Immunology 2024Quote: ... incubated for 4 minutes at room temperature, centrifuged (3 min, 500g, 4C) and re-suspended with FACS buffer containing CD16/CD32 blocking antibodies (BioLegend) for 15 minutes on ice ...
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bioRxiv - Microbiology 2024Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Genomics 2024Quote: ... Cells were then cultured for 12-18h in DMEM/10%FBS supplemented with 100ng/mL mSCF/mIL-3/mIL-6 (BioLegend) at 37°C at 5% CO2 [Step Four] ...
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bioRxiv - Neuroscience 2021Quote: ... Alexa Fluor 488 anti-CD54 antibody (2 μg/mL, ICAM-1, YN1/1.7.4, BioLegend), and Alexa Fluor 647 anti-CD106 antibody (2 μg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... we used an antibody against the Rpb3 subunit (2 μl per reaction, Biolegend 665004) conjugated to 20 μl protein G magnetic beads (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Splenocytes were plated at 2 million cells/well and incubated with Zombie UV (Biolegend) for dead cell discrimination ...
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bioRxiv - Immunology 2021Quote: ... 0.1% 2-mercapto-ethanol 1000X solution) supplemented with IL-7 (10 ng/mL, Biolegend). To activate T cells ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then washed with 2 ml of cell staining buffer (Cat. #420201, BioLegend), resuspended in 300 μl PBS and measured using an LSR II flow cytometer (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... Cells (2 x 105) were incubated with PerCP-anti-CD4 (clone A161A1) antibody (Biolegend) for 15 min at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... These microglia were labeled with FITC anti-mouse CD45 (2 µg/mL, Biolegend, USA), APC anti-mouse CD11b (5 µg/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM EDTA) and stained with Zombie Aqua Fixable Viability Kit (BioLegend, 1:200). Living ...
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bioRxiv - Cell Biology 2021Quote: ... EVs were then pulled down by incubating with anti CD81-Biotin (Eat-2, Biolegend) coated streptavidin beads overnight rotating at 4°C (Exobeads ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 μM (in vitro) and neutralizing HMGB1 of 10µg/ml were obtained from Biolegend (isotype control mouse IgG2a kappa ...
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bioRxiv - Immunology 2020Quote: ... supernatants were harvested and TNF and IL-2 levels were measured by ELISA (Biolegend) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... B cells were labelled with FITC-labelled anti-IgD antibody (BioLegend clone IA6-2) per manufacturer’s instructions and FITC positive populations were removed ...
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bioRxiv - Immunology 2020Quote: 2 µg of primary conjugated anti-mouse Ly6G-Alexa Fluor 647 (clone 1A8, Biolegend) diluted into 200 µl PBS were injected intravenously via tail vein ten minutes prior to imaging as previously published [15,16].
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed with PBS/2% FBS and stained with streptavidin conjugated BV605 (BioLegend) if CD90 biotin-conjugated antibody was used ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in 100 μl of FACS buffer with 2 μl 7AAD (BioLegend) and incubated in the dark on ice for a further 5 minutes ...
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bioRxiv - Immunology 2021Quote: ... and anti-CD32-PE-Cy7 (clone: FUN-2) (all from Biolegend, San Diego, CA), and anti-CD206-APC-Vio 770 (clone ...
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bioRxiv - Immunology 2021Quote: ... and Dectin-2 expression were examined by using rat anti-mouse Dectin-1 (Biolegend) followed by Alexa-594-conjugated goat anti-rat (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... CD18 (M18/2) and β7 (FIB 504) were purchased from BioLegend (San Diego, CA). Secondary AlexaFluor-labelled antibodies were from Jackson ImmunoResearch (West Grove ...
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bioRxiv - Neuroscience 2023Quote: ... PE rat anti-mouse CD11b (cat # 101208, BioLegend, clone M1/70, 2 ng/μl), APC rat anti-mouse ACSA-2 (cat # 130-117-535 ...
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bioRxiv - Immunology 2023Quote: ... Leukocytes were blocked with 2% normal mouse serum and 1% FcX Block (BioLegend, #101320) in FACS buffer for 30 minutes at 4°C prior to being stained with fluorescently conjugated antibodies to CD3e (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... and secretion of IFNγ and IL-2 were measured by ELISA (ELISA MAX, BioLegend) in supernatant removed after 24-hour incubation.
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bioRxiv - Immunology 2023Quote: ... biotinylated SARS-CoV-2 Spike RBD protein was mixed with Streptavidin-BV421 (405225, BioLegend) at a 4:1 molar ratio for 1 hour at 4 °C to obtain the antigen probe ...
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bioRxiv - Genomics 2024Quote: ... antibody for Th1 conditions or 10ng/mL rhIL-2 and 5ng/mL rhTGFý (Biolegend) for Treg conditions.
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bioRxiv - Developmental Biology 2024Quote: ... 2 µL (1 µg) of the corresponding hashing antibody (Totalseq-A, Biolegend, Table S4) were added to the blocked samples ...
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bioRxiv - Immunology 2024Quote: ... plates were coated with 2 µg/mL of anti-IgE (clone RME-1, BioLegend) and for IgG1 ELISAs ...
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bioRxiv - Immunology 2024Quote: ... Purified mouse anti-SARS-CoV-2 spike protein S2 antibody (1:3000 dilution, BioLegend) and HRP-conjugated goat anti-mouse secondary antibody (1:3750 dilution ...
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bioRxiv - Immunology 2023Quote: ... Antibodies used for studies related to the Fra-2 model were purchased from Biolegend. Assessment of fibrosing alveolitis by Micro-CT images were obtained with a Perkin Elmer Quantum FX system (Caliper Life Sciences ...
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bioRxiv - Immunology 2023Quote: ... H-2Dd (Anti-mouse H-2Dd antibody; clone 34-2-12; Biolegend; Cat #110606), CD25 (BV510 Rat Anti-Mouse CD25 ...