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Citations for BioLegend :
701 - 750 of 1319 citations for 3 2 Methylphenoxy methyl piperidine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... α-IL-2-AlexaFluor700 (1:200; Cat.-No. 503818, Biolegend, San Diego, USA) and α-TNF-α-Pe-Cy7 (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... or 2 μg ml−1 IL-6 neutralizing antibody (MP5-20F3, Biolegend) at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 ΔRBM RBD-PE/Cy5.5 (streptavidin-PE/Cy5.5 from BioLegend). The panel for the cohort that received the SARS-CoV-2 spike prime followed by the RBD homotrimer cocktail boost also included SARS-CoV RBD-APC (streptavidin-APC from BioLegend ...
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bioRxiv - Immunology 2021Quote: ... and anti-mouse-IL-2-PE-Cy7 (Biolegend Cat# 503832, RRID: AB_2561750). Stained cells were acquired by FACSAriaIII Flow Cytometer (BD Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... and AlexaFluor-647-conjuaged anti-CD18 antibody (M18/2, Biolegend, 1:100). Cells were washed with FACS buffer ...
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bioRxiv - Pathology 2021Quote: ... pan aSyn (N-terminal directed, SYN303 [aa 2-12 Cat# 82401, Biolegend] ...
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bioRxiv - Cell Biology 2021Quote: ... and 2 μg/ml soluble anti-mouse CD28 antibody (102116, BioLegend, USA) in RPMI 1640 medium (21870-076 ...
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bioRxiv - Immunology 2021Quote: ... PE-anti-human-Nectin-2 (TX31) (all from BioLegend, San Diego, CA). The following antibodies were used for in vivo blocking experiments ...
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bioRxiv - Immunology 2020Quote: ... KLRG1 APC-Cy7 (clone 2FI/KLRG1, BioLegend, cat# 138426, 2:100 dilution); CD279 (PD-1 ...
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bioRxiv - Immunology 2020Quote: ... CD279 (PD-1) BV650 (clone EH12.2H7, BioLegend, cat# 329950, dilution 2:100); CD45RA BV510 (clone HI100 ...
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bioRxiv - Immunology 2022Quote: ... 1-2×106 cells were incubated with Human TruStain FcX (BioLegend #422302) and Fixable Viability Stain 510 (BD #564406 ...
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bioRxiv - Neuroscience 2022Quote: ... Fisher) along with 2 µl of live/dead cell stain (DRAQ7, BioLegend) per ml and then FACS sorted.
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bioRxiv - Immunology 2022Quote: ... Rat anti-CD138 antibody (rat anti-CD138, clone 281-2, Biolegend, 142502) was applied to each section and specific bounds were visualized using a Alexa Fluor488-conjugated anti-rat antibody (JacksonImmunoResearch ...
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bioRxiv - Immunology 2024Quote: ... Media was additionally supplemented with 10 ng/mL murine IL-2 (BioLegend) prior to resuspension and subsequent passaging ...
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bioRxiv - Immunology 2024Quote: ... 1-2×106 cells were incubated with Fc receptor blocking reagent (BioLegend) for 5 min at RT ...
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bioRxiv - Immunology 2024Quote: ... and with 100 U/ml IL-2 (BioLegend, San Diego, CA, USA) (referred to as “Bead activated T cells” or “nonspecific activation” ...
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bioRxiv - Immunology 2024Quote: ... the gels were incubated with 2 μg/ml Calcein-AM (BioLegend, 425201) and 1 μg/ml Propidium Iodide (MP Biomedicals ...
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bioRxiv - Neuroscience 2023Quote: ... or 2 ug/ml PE-conjugated anti-mouse TLR2 antibodies (BioLegend, 148603) for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... anti-CD138 Alexa Flour 647 (1:80 dilution, clone 281-2, BioLegend), donkey anti-mouse IgG Alexa Fluor Plus 405 (1:200 dilution ...
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bioRxiv - Immunology 2023Quote: ... Culture supernatants were collected and analyzed for IL-2 by ELISA (BioLegend).
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bioRxiv - Immunology 2023Quote: ... Cells were washed with 2× Cyto-FastTM Perm Wash solution (CFPWS; BioLegend) and incubated with PGP9.5 for 20 min at RT ...
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bioRxiv - Immunology 2024Quote: ... 2 hours after start of restimulation 5μg/mL of Brefeldin A (BioLegend) was added to each well for the remaining 3 hours ...
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bioRxiv - Biochemistry 2024Quote: ... An anti-pERK1/2 (Thr202/Tyr204) antibody (6B8B69) was purchased from BioLegend. Dead cells were stained with the LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit for 633- or 635-nm excitation (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... cells were stained with 2 µl of TotalSeqTM-C hashing antibodies (Biolegend) in wash buffer for 30 min at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... permeabilized and stained with anti-IL-2 antibody (Clone, JES6-5H4, Biolegend) and analyzed using a BD LSR Fortessa (BD Biosciences ...
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bioRxiv - Bioengineering 2024Quote: ... and cells were cultured with 0.5 µg/mL recombinant IL-2 (BioLegend) in RPMI + 10% heat-inactivated FBS for 24 h before use in NK cell cytotoxicity or flow cytometry experiments.
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bioRxiv - Immunology 2024Quote: ... T cells were supplemented with 2 µg/mL anti-CD28 (37.51; Biolegend), seeded in a F-bottom 96-well plate precoated with 3 µg/mL anti-CD3 (145-2C11 ...
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bioRxiv - Bioengineering 2024Quote: ... antibody while staining with B2M (Beta-2 Microglobulin) (1:200, 316312 BioLegend) antibody for 60 min in the dark at +4⁰C to identify pluripotency state and B2M KO of the cells before acquiring on flow cytometer.
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bioRxiv - Cancer Biology 2021Quote: ... Cell lines or expanded primary NK cells were stimulated with 3 ug/mL CD28 activating antibody (Biolegend, cat#302933) for 24 hours.
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bioRxiv - Immunology 2021Quote: ... spleen T cell IL-1β/IL-23 plus spermidine treatment group (SPT sper) with hashtag 3 (BioLegend, Cat# 155805); lymph node cell IL-1β/IL-23 stimulation group (LN stim ...
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bioRxiv - Physiology 2022Quote: ... Cells were then washed in HBSS with 3% BSA and incubated with CD34 Brilliant Violet 421 (BioLegend 230 119321) at 1:400 and CD24 PerCP-Cyanine 5.5 (eBioscience ...
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bioRxiv - Genomics 2021Quote: ... for RNA library construction while modifications for CITE-seq ADT and cell hashing HTO library preparation were performed according to the TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 or v3.1 single index protocol (BioLegend). The modifications include the addition of ADT and HTO additive primers to a final concentration of 0.2 uM in the cDNA amplification reaction (step 2.2) ...
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bioRxiv - Immunology 2022Quote: ... added for last 3 hrs of stimulation and stained with IFN-γ (XMG1.2; eBioscience) and IL-17A (TC11-18H10.1; Biolegend) using a Cytofix/Cytoperm kit (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... These cells were stimulated with 10μg/ml of anti-CD3 (OKT-3) and anti-CD28 (28.2) antibodies (Ultra-LEAF™, Biolegend) in R10-50-15 for 48 hours ...
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bioRxiv - Bioengineering 2020Quote: ... dilutions of the serum were mixed with a constant 3 nM of an HRP conjugated anti-OVA antibody (BioLegend) and incubated for 2 h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... and seeded in the antibody-coated flask at 3–5×105 cells/cm2 together with soluble 1 µg/mL anti-CD28 antibodies (clone 37.51; 102116, BioLegend). Cells were cultured for 48 hr at 37°C in a humidified 5% CO2 atmosphere ...
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bioRxiv - Immunology 2022Quote: ... mice were injected into the retro-orbital sinus with a total of 3 mg of PE/Cy7-CD45.2 (104, BioLegend) in 200 μl sterile DPBS ...
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bioRxiv - Synthetic Biology 2024Quote: ... 50,000 CAR-T cells were co-cultured with 100,000 target cells for 5h in the presence of CD107a antibody (Supp. Table 3) and 1x Brefeldin A (Biolegend). Following co-culture ...
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bioRxiv - Immunology 2024Quote: ... intestine and skin (n = 3, PCW 9.5) were stained with the following antibodies (clone, fluorophore, company): CD3 (UCHT1, FITC, Biolegend), CD7 (M-T701 ...
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bioRxiv - Immunology 2024Quote: ... which is RPMI-1640-based and supplemented with 5 μg/mL recombinant mouse IL-3 (#575506 or #575508, BioLegend) as described in previous studies (6 ...
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bioRxiv - Immunology 2024Quote: Single cell suspensions from 3 B16-OVA WT tumours were labelled with TotalSeq(TM)-C0301 antibody (Biolegend, Cat. 155861), and 3 IFNγRKO tumours were labelled with TotalSeq(TM)-C0302 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... coupled with the protocol TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 or v3.1 (Single Index) from BioLegend.
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bioRxiv - Immunology 2023Quote: ... 130-120-662) for 3 days in 24-well plates coated with 3μg/ml anti-CD3 (145-2c11; Biolegend) and 3μg/ml anti-CD28 (37.51 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... For the HashTag library TotalSeq™-B0303 anti-mouse Hashtag 3 Antibody (Clone M1/42; 30-F11; 155835; BioLegend) was used to mark single cells of DMSO treated mice and TotalSeq™-B0304 anti-mouse Hashtag 4 Antibody (Clone M1/42 ...
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bioRxiv - Immunology 2024Quote: ... with the addition of either DCs (1 cell:3 Tresp) with 0.5 μg/ml purified anti-CD3 (145-2C11, Biolegend), or Dynabeads Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation (Gibco ...
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bioRxiv - Immunology 2024Quote: ... about 3-4 million CD8+ T cells were blocked with Fc receptor blocking solution (Human TruStain FcX, BioLegend, #422302) for 10 minutes on ice and stained with BEAM-T assemblies for 15 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... anti-IgD-FITC (1:20 dilution, clone IA6-2, cat. no. 348206, BioLegend), anti-IgM-FITC (1:20 dilution ...
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bioRxiv - Microbiology 2021Quote: ... in the presence of 2 μg/mL soluble αCD28 antibody(clone CD28.2, Biolegend). RNAi knockdown of DCAF1 was performed as described before52 using ON-TARGET plusHuman DCAF1 siRNA - SMARTpool (Dharmacon ...
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bioRxiv - Bioengineering 2022Quote: ... and 50 μg/mL anti-IL-2 Alexa Fluor 488 (Biolegend, Catalog: 500314) in 50μL washing buffer per 6 μL nanovial volume at 37°C for 30 minutes protected from light ...
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bioRxiv - Immunology 2020Quote: ... Supernatants were collected to measure IL-2 by ELISA (ELISA MAX Deluxe, BioLegend) and proliferation assessed by methyl-3[H]-thymidine (1 µCi/well ...