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Citations for BioLegend :
951 - 1000 of 1319 citations for 3 2 Methylphenoxy methyl piperidine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... cell suspensions were resuspended in PBS containing 2 % BSA and a viability dye (Zombie Red, Biolegend) for discrimination between live and dead cells ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with 2 ug/ml PE/Cyanine7-conjugated anti-mouse TLR4 antibodies (BioLegend, 145407) or 2 ug/ml PE-conjugated anti-mouse TLR2 antibodies (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... A total of 2 × 105 donor-matched PBMCs or ToMCs were labeled with CFSE (BioLegend #423801), pre-treated with different inhibitors for 30 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... immunoprecipitation was conducted for the supernantant as described above using 2 µg of M3.2 antibody (Biolegend) and 50 µl Protein G sepharose.
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bioRxiv - Immunology 2023Quote: IL-2 concentrations in the supernatant were measured by enzyme-linked immunosorbent assay (ELISA) from BioLegend. For the CRISPR screen ...
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bioRxiv - Immunology 2024Quote: ... Supplementary Table 1) and 1ul/staining volume of hashtag TotalSeqC antibody mastermix (Biolegend, Supplementary Table 2) was added to each sample and cells were incubated for 30 minutes at 4°C ...
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bioRxiv - Immunology 2024Quote: ... and then stained 1h in PBS 2% BSA + 10µg/mL 2.4G2 with anti-vimentin AF647 (Biolegend) and counterstained using 500ng/mL DAPI dilactate (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... The supernatant was used to determine the IL-2 secretion using the IL2 Elisa kit (#BioLegend), and the cells were used to determine the protein concentration using BCA assay (#Thermo ...
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bioRxiv - Neuroscience 2024Quote: ... Directly conjugated antibodies used were MDL-1-PE (Clone 14H4-1-2; 1:200; Biolegend # 371705), mouse IgG2b-PE isotype control (Clone MPC-11 ...
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bioRxiv - Immunology 2024Quote: ... PBMCs (2×106 cells/ml) were stained with 0.5 μM CFSE (BioLegend, SanDiego, CA, USA; B377681) in PBS 1X (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... 2 million cells of each sample were resuspended in 100 µL Cell Staining Buffer (420201, BioLegend) supplemented with 10% fetal bovine serum (F2442 ...
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bioRxiv - Cancer Biology 2024Quote: ... IL-22 were quantified using the LEGENDplex™ Human T Helper Cytokine Panels Version 2 (BioLegend) according to the manufacturer’s recommendations.
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bioRxiv - Bioengineering 2021Quote: ... T cells were similarly isolated and stained with Ghost Dye and lineage/differentiation markers (without CD19) and with TIM-3 BV711 (Biolegend clone F38-2E2), LAG-3 BV421 (Biolegend clone 11C3C65) ...
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bioRxiv - Cancer Biology 2024Quote: ... Spleenocytes were resuspended in 3 ml MojoSort™ buffer and enriched for CD8+ T cells using the MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend, 480035), following manufactureŕs instructions.
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bioRxiv - Microbiology 2024Quote: ... IL-10, IL-12p70, IFN-α2, IFN-β, IFN-λ1, IFN-λ2/3, IFN-γ, TNF-α, IP-10 and GM-CSF; BioLegend) assay was performed on patient plasma samples as per the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... The cells were expanded for additional 3 days and Siglec+ population was sorted by staining cells with anti-Siglec-7 APC (BioLegend, clone 6-434) or anti-Siglec-9 APC (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... all peptide-stimulated PBMCs were stained with TotalSeqTM-A 0390 anti-human CD127 - and TotalSeqTM-C 0085 anti-human CD25 oligo-antibodies (BioLegend, Supplementary table 3) to distinguish between conventional T cells (CD127+CD25- ...
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bioRxiv - Biophysics 2021Quote: Flow channels were constructed as previously described but anti-TAMRA antibody was replaced with Anti-ß3 tubulin antibody (BioLegend Poly18020 anti-Tubulin Beta 3). Microtubule nucleation was achieved by introducing 5-50 μM tubulin (1:17 molar ratio of Atto-633 labelled:unlabelled ...
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bioRxiv - Neuroscience 2020Quote: The cerebellar slices were treated either 3 times 24 hours apart with combinations of recombinant TNF (recombinant rat TNF, Biolegend, San Diego, CA, USA), LTα (recombinant human LT-α ...
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bioRxiv - Cell Biology 2022Quote: ... half a million cells per sample were incubated for 10 min in 50 μl of PBS with 3% FSB and 5 μl of blocking buffer (BioLegend, San Diego, CA, USA) and then stained with 50x diluted TotalSeq-B cell feature barcode antibody (BioLegend ...
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used in this study: anti-tubulin beta-3 conjugated to Alexa Fluor 488 (BioLegend; AB_2562669; mouse IgG2a, 1:200), Alexa Fluor 555 (BD PharMingen ...
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bioRxiv - Cancer Biology 2023Quote: Other conjugated antibodies used in this study include the following: Alexa Fluor® 647 anti-Tubulin Beta 3 (1:1000, Biolegend, #657406, clone AA10), Alexa Fluor® 488 anti-Vimentin (1:500 ...
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bioRxiv - Immunology 2021Quote: ... spleen T cell IL-1β/IL-23 stimulation group (SPT stim) with hashtag 2 (BioLegend, Cat# 155803); spleen T cell IL-1β/IL-23 plus spermidine treatment group (SPT sper ...
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bioRxiv - Molecular Biology 2021Quote: ... or day 5 IL-2 cultured NK cells isolated using the MojoSort NK isolation kit from Biolegend, but instead of analysis by flow cytometry ...
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bioRxiv - Neuroscience 2021Quote: ... Alexa Fluor 594 or Alexa Fluor 647 conjugated anti-mouse CD31 (2 μg/mL; clone MEC13.3; BioLegend) was used ...
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bioRxiv - Immunology 2022Quote: ... media was supplemented with 2 μg/ml of mouse IgG1 K anti-human PD-1 antibody (Biolegend) either alone or in combination with 1 μg/ml mouse IgG2 B anti-human TIGIT (R&D systems ...
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bioRxiv - Immunology 2021Quote: ... and cells were instead stained with 4′,6-diamidino-2-phenylindole (DAPI; BioLegend, San Diego, CA, USA) and gated for live (DAPI- ...
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bioRxiv - Immunology 2021Quote: ... IFN-β and IFN-γ were determined using the LEGENDplex mouse type 1/2 interferon panel (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... we incubated the nanowell array with 4 µg/ml PE anti-CD63 antibody (BioLegend, clone NVG-2) for 45 minutes at 37°C ...
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bioRxiv - Immunology 2022Quote: ... as well as SARS-CoV-2-S1 (biotin-coupled; Acro; #S1N-C82E8) and fluorochrome-coupled streptavidin (Biolegend). The anti-ST6GAL1 and anti-FUT8 Abs were labeled with Alexa Fluor 488 labeling kit (Life Technologies GmbH ...
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bioRxiv - Immunology 2020Quote: ... frozen PBMC were thawed and stained with 2 μl of anti-human CD3-AF488 (clone UCHT1, Biolegend) and 1 μl of anti-human CD14-PE (clone 61D3 ...
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bioRxiv - Neuroscience 2022Quote: ... Pre-centrifuged 50 μL of hashtag antibody solution containing 2 uL of TotalSeq-A Hashtag antibody (BioLegend), with a unique barcode sequence for each iPS cell line ...
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bioRxiv - Physiology 2022Quote: ... mice were intravenously injected with 2 μg of Alexa Fluor 647 anti-mouse CD127 antibody (A7R34, BioLegend) and euthanized 5 minutes after injection ...
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bioRxiv - Immunology 2022Quote: ... The abundance of Nectin-2+ monocytes and NK cell intracellular cytokine production of IFN-γ (4S.B3, Biolegend) were analyzed by flow cytometry (BD LSRFortessa™ Cell Analyzer ...
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bioRxiv - Immunology 2022Quote: ... or plate-bound α-CD3ε (2 μg/mL) and plate-bound CD155-Fc (1 μg/mL; BioLegend) for four days ...
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bioRxiv - Immunology 2022Quote: Cells were stained for flow cytometry using the following antibodies diluted in FACS Buffer (2% FBS, 0.01% Sodium Azide, PBS): BV711-anti-CD326 (EpCAM) (Clone:G8.8, BioLegend), BUV395-anti-CD45.2 (Clone:104 ...
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bioRxiv - Cancer Biology 2020Quote: ... 100,000–500,000 single cells of breast tumours were suspended in 100μl staining buffer (2% BSA, 0.01% Tween in PBS) before adding 10µl Fc-blocking reagent (FcX, BioLegend). and incubating during 10 min on ice ...
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bioRxiv - Immunology 2020Quote: ... anti-human BDCA-2-PE (clone AC144; Miltenyi Biotech) and anti-human CD123-APC (clone 6H6; Biolegend). Cells were incubated with surface antibodies at 4°C for 30 minutes ...
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bioRxiv - Genomics 2021Quote: ... Cells sorted for IL-2 were treated with Cell Activation Cocktail with Brefeldin A (Biolegend, Cat #423304) for 4 hours prior to fixation ...
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bioRxiv - Immunology 2020Quote: ... or 2) 10 μg/mL αLFA-1 + 10 μg/mL antibodies against CD16 (αCD16; BioLegend, clone 3G8). All loading solutions were mixed in PBS and supplemented with 10 μg/mL bovine serum albumin (BSA ...
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bioRxiv - Immunology 2021Quote: ... CD8 BUV496 or BV650 (2:100 or 4:100; BD Biosciences Cat# 612942 or Biolegend Cat# 301042), and Live/Dead eFluor506 (5:1000 ...
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bioRxiv - Immunology 2022Quote: ... B cells were stimulated for 2 days with 10 ug/ml of anti-CD40 (Biolegend, Cat#102812) and 10 ng/ml of rec ...
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bioRxiv - Immunology 2022Quote: ... in the presence of rhIL-2 (50U/ml, Novartis) and anti-IFNγ (5µg/ml, clone XMG1.2, Biolegend). For intracellular cytokine staining ...
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bioRxiv - Immunology 2022Quote: ... in the presence or absence of a mouse anti-human PD1 (2 μg/mL) blocking antibody (Biolegend). CD4+ T-cells were identified using a mouse-anti human CD4 (OKT4 clone ...
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bioRxiv - Immunology 2022Quote: ... and MDA-231 cells were stained with APC conjugated anti-human CD340 (erbB2/HER-2) Antibody (Biolegend). MR1 expression on T2 cells was assessed with anti-MR1-PE (Biolegend) ...
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bioRxiv - Immunology 2024Quote: ... Cells were fixed with 2% paraformaldehyde (Pierce, 28906) diluted in PBS or commercial fixation buffer (Biolegend, 420801) for 20 minutes on ice ...
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bioRxiv - Genomics 2024Quote: ... at a 1:300 dilution with DAPI 2 μl in 1 ml of 10 μg/ml (BioLegend) and kept on light rotation for 2 h at RT ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed once and resuspended in 100μl blocking buffer (2%FBS and 1% FcX (BioLegend, 422302) in PBS ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies were used at a concentration of 1:100: CD14 (BioLegend, #123312, clone Sa14-2), CD86 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... and penicillin in a 96-well plate pre-coated with anti-CD3Ab (2 μg/ml, Biolegend 100360), along with the addition of 5 μg/ml anti-CD28 antibody (Biolegend 102122 ...