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• Hypoxic memory of tumor intrinsic type I interferon suppression promotes breast cancer metastasis

  Oihana Iriondo, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Cells were then fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Biolegend) before intracellular staining of Arginase I ...
• Hypoxic memory of tumor intrinsic type I interferon suppression promotes breast cancer metastasis

  Oihana Iriondo, et al., bioRxiv - Cancer Biology 2023

  Quote: ... F4/80 (Biolegend 123141), MHCII (BD Biosciences 562009) ...
• Unifying comprehensive genomics and transcriptomics in individual cells to illuminate oncogenic and drug resistance mechanisms

  Jeffrey R. Marks, et al., bioRxiv - Genomics 2023

  Quote: ... Following Calcein AM (BioLegend 425201), propidium iodide (Millipore Sigma P4864 ...
• Transferrin Receptor is Required for CD4+ T Cell Iron-Dependent Response During Acute Toxoplasma gondii Infection

  Stephen L. Denton, et al., bioRxiv - Immunology 2023

  Quote: ... Ly6C-PE (HK1.4, Biolegend 128008), Ly6G-PE/Cy7 (1A8 ...
• Deletion of the protein tyrosine phosphatase PTPN22 for adoptive T cell therapy facilitates CTL effector function but promotes T cell exhaustion

  Alexandra R. Teagle, et al., bioRxiv - Immunology 2023

  Quote: ... Cells were labelled with live/dead and surface stains prior to fixation and permeabilization with fixation buffer (Biolegend) and intracellular staining permeabilization wash buffer (Biolegend) ...
• Androgen exposure impairs neutrophil maturation and function within the infected kidney

  Teri N. Hreha, et al., bioRxiv - Immunology 2023

  Quote: ... CXCR2-PE/Cy7 (1:200, Biolegend #149316). Cells were washed ...
• Purinergic Preconditioning Induces Epigenomic and Transcriptomic Changes Resembling Epilepsy-associated Microglial States

  Ricardo Martins-Ferreira, et al., bioRxiv - Immunology 2023

  Quote: ... CD68 (APC-Cy7) (#333822 BioLegend), CD45 (KO ...
• A Th17 cell-intrinsic glutathione/mitochondrial-IL-22 axis protects against intestinal inflammation

  Lynn Bonetti, et al., bioRxiv - Immunology 2023

  Quote: ... soluble anti-CD28 (1µg/mL; Biolegend), plate-bound anti-CD3 antibody (5μg/mL ...
• Single-cell analysis of signalling and transcriptional responses to type I interferons

  Rachel E. Rigby, et al., bioRxiv - Immunology 2023

  Quote: ... BSA/0.01% (v/v) Tween-20 in cold RNase-free PBS + 10 μl Human TruStain FcX blocking solution (#422301, BioLegend)) and incubated for 10 min at 4°C ...
• Mechanical Force Imprints Neutrophils to Orchestrate Pulmonary Homeostasis

  Jin Wang, et al., bioRxiv - Immunology 2023

  Quote: ... or 30 µL 1% FITC-Dextran70 (TdB Labs, 21024) to visualize vasculature and 7 µL APC-Ly6G (Biolegend, 127614) antibody to label neutrophil 30 min before imaging.
• Sequential Switching Through IgG1 is Redundant for Allergic Reactivity and Memory to Allergens

  Joshua F.E. Koenig, et al., bioRxiv - Immunology 2023

  Quote: ... or biotinylated anti-mouse IgG (H+L) (Poly4053, Biolegend, 405301) for 2 hours at RT ...
• Triphasic production of IFNγ by innate and adaptive lymphocytes following influenza A virus infection

  George E Finney, et al., bioRxiv - Immunology 2023

  Quote: ... anti-ICOS BV785 (BioLegend; clone: C398.4A), anti-NK1.1 APC-Cy7 (BioLegend ...
• Establishment of CD1b-restricted immunity to lipid antigens in the pulmonary response to Mycobacterium tuberculosis infection

  Macallister C. Harris, et al., bioRxiv - Immunology 2023

  Quote: ... Viability of cells was assessed via Zombie Yellow amine-reactive permeability dye (BioLegend, Cat# 423103). Data were acquired using a spectral unmixing Cytek Aurora flow cytometer (Cytek ...
• Triphasic production of IFNγ by innate and adaptive lymphocytes following influenza A virus infection

  George E Finney, et al., bioRxiv - Immunology 2023

  Quote: ... anti-γδTCR PE-Cy7 (BioLegend; clone: GL3), anti-ICOS BV785 (BioLegend ...
• Triphasic production of IFNγ by innate and adaptive lymphocytes following influenza A virus infection

  George E Finney, et al., bioRxiv - Immunology 2023

  Quote: ... or PerCP-Cy5.5 (ThermoFisher; B220 and F4/80, and BioLegend; MHCII). Cells were stained with a fixable viability dye eFluor 506 (ThermoFisher) ...
• Cross-Protection Induced by Highly Conserved Human B, CD4^+, and CD8⁺ T Cell Epitopes-Based Coronavirus Vaccine Against Severe Infection, Disease, and Death Caused by Multiple SARS-CoV-2 Variants of Concern

  Swayam Prakash, et al., bioRxiv - Immunology 2023

  Quote: ... the cells were stained with a live/dead fixable dye (Zombie Red dye, 1/800 dilution – BioLegend, San Diego, CA) for 20 minutes at room temperature ...
• Triphasic production of IFNγ by innate and adaptive lymphocytes following influenza A virus infection

  George E Finney, et al., bioRxiv - Immunology 2023

  Quote: ... anti-NK1.1 APC-Cy7 (BioLegend; clone: PK136), anti-PD1 BV711 (BioLegend ...
• Tempered IL-2 Signals Program PD-1 Checkpoint Blockade-Responsive Stem-like Exhausted T Cells During Priming

  Vandana Kalia, et al., bioRxiv - Immunology 2023

  Quote: ... cells were enriched from total splenocytes using the EasySep Mouse CD8+ T cell Isolation Kit (Biolegend) at day 3.5 post infection ...
• Triphasic production of IFNγ by innate and adaptive lymphocytes following influenza A virus infection

  George E Finney, et al., bioRxiv - Immunology 2023

  Quote: ... Antibodies used were: anti-CD3 BV785 (BioLegend; clone: 17A2), anti-CD4 BUV805 (BD Bioscience ...
• Cross-Protection Induced by Highly Conserved Human B, CD4^+, and CD8⁺ T Cell Epitopes-Based Coronavirus Vaccine Against Severe Infection, Disease, and Death Caused by Multiple SARS-CoV-2 Variants of Concern

  Swayam Prakash, et al., bioRxiv - Immunology 2023

  Quote: ... Plates were washed 4 times with PBS and 40μl of HRP anti-rabbit IgG antibody (Biolegend) 1:1500 was added to each well and incubated for 2 hours at room temperature ...
• Triphasic production of IFNγ by innate and adaptive lymphocytes following influenza A virus infection

  George E Finney, et al., bioRxiv - Immunology 2023

  Quote: ... anti-CD8b APC (BioLegend, clone; 53-5.8), anti-EpCAM AF594 (BioLegend ...
• Tempered IL-2 Signals Program PD-1 Checkpoint Blockade-Responsive Stem-like Exhausted T Cells During Priming

  Vandana Kalia, et al., bioRxiv - Immunology 2023

  Quote: All antibodies were purchased from Biolegend (San Diego, CA, USA) except for TCF-1 (Cell signaling ...
• Triphasic production of IFNγ by innate and adaptive lymphocytes following influenza A virus infection

  George E Finney, et al., bioRxiv - Immunology 2023

  Quote: ... anti-CD45.2 BV605 (BioLegend; clone: 104), anti-CD69 PE-Cy7 (ThermoFisher ...
• Triphasic production of IFNγ by innate and adaptive lymphocytes following influenza A virus infection

  George E Finney, et al., bioRxiv - Immunology 2023

  Quote: ... anti-PD1 BV711 (BioLegend; clone: 29F,1A12), and ‘dump’ antibodies ...
• Establishment of CD1b-restricted immunity to lipid antigens in the pulmonary response to Mycobacterium tuberculosis infection

  Macallister C. Harris, et al., bioRxiv - Immunology 2023

  Quote: ... Cells were placed in 4 ml of 1X annexin binding buffer (Biolegend, cat#: 422201) with biotinylated annexin V (Biolegend ...
• An autophagy program that promotes T cell egress from the lymph node controls responses to immune checkpoint blockade

  Diede Houbaert, et al., bioRxiv - Immunology 2023

  Quote: ... anti-KLRG1 (138423, Biolegend), anti-GranzymeB (130-116-486 ...
• An autophagy program that promotes T cell egress from the lymph node controls responses to immune checkpoint blockade

  Diede Houbaert, et al., bioRxiv - Immunology 2023

  Quote: ... anti-CD19 (115545, Biolegend), anti-NK1.1 (746876 ...
• Regulation of BCR-mediated Ca²⁺ mobilization by MIZ1-TIMBIM4 safeguards IgG1⁺ GC B cell positive selection

  Lingling Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... followed by intracellular staining with True-Nuclear transcription factor buffer set (Biolegend) for further fixation and permeabilization ...
• CARs are organized in nanodomains in the plasma membrane of T cells that accumulate at tumor contact sites

  Christina Verbruggen, et al., bioRxiv - Immunology 2023

  Quote: ... and secretion of IFNγ and IL-2 were measured by ELISA (ELISA MAX, BioLegend) in supernatant removed after 24-hour incubation.
• Soluble CTLA-4 mainly produced by Treg cells inhibits type 1 inflammation without hindering type 2 immunity to allow for inflammation resolution

  Motonao Osaki, Shimon Sakaguchi, bioRxiv - Immunology 2023

  Quote: ... anti-mCD38 (90, eBioscience, 17-0381-82, or BioLegend, 102714), anti-h/mGL-7 (GL-7 ...
• Soluble CTLA-4 mainly produced by Treg cells inhibits type 1 inflammation without hindering type 2 immunity to allow for inflammation resolution

  Motonao Osaki, Shimon Sakaguchi, bioRxiv - Immunology 2023

  Quote: ... anti-mCD68 (FA-11, BioLegend, 137017), anti-mCD64 (X54-5/7.1 ...
• Soluble CTLA-4 mainly produced by Treg cells inhibits type 1 inflammation without hindering type 2 immunity to allow for inflammation resolution

  Motonao Osaki, Shimon Sakaguchi, bioRxiv - Immunology 2023

  Quote: ... anti-mCD206 (C068C2, BioLegend, 141705), anti-mCD163 (S15049I ...
• Soluble CTLA-4 mainly produced by Treg cells inhibits type 1 inflammation without hindering type 2 immunity to allow for inflammation resolution

  Motonao Osaki, Shimon Sakaguchi, bioRxiv - Immunology 2023

  Quote: ... anti-mCD64 (X54-5/7.1, BioLegend, 139304), anti- mCD71 (RI7217 ...
• Distinct Functional States of Neutrophils by Actin Disassembly and NF-kB/STAT3 Signaling

  Niko Heineken, et al., bioRxiv - Immunology 2023

  Quote: ... After washing cells were fixed and permeabilized using the True-Nuclear™ Transcription Factor Buffer Set (BioLegend) followed by intracellular staining for 30 minutes at room temperature ...
• Distinct Functional States of Neutrophils by Actin Disassembly and NF-kB/STAT3 Signaling

  Niko Heineken, et al., bioRxiv - Immunology 2023

  Quote: ... Zombie NIR (BioLegend) was used to exclude dead cells according to the Manufacturer’s instructions ...
• High-resolution map of the Fc-functions mediated by COVID-19 neutralizing antibodies

  Ida Paciello, et al., bioRxiv - Immunology 2023

  Quote: ... Cells were fixed with fixation buffer (Biolegend) for 15 minutes on ice ...
• Mmp17-deficient mice exhibit heightened goblet cell effector expression in the colon and increased resistance to chronic Trichuris muris infection

  Pia M. Vornewald, et al., bioRxiv - Immunology 2023

  Quote: ... The antibody solution was then removed and the isolated lymph node cells were added at 2×105 cells per well together with soluable anti-mouse CD28 (102112, Biolegend) at a final concentration of 2 µg/ml ...
• Selective inhibition of fibroblast-specific Domain Discoidin Receptor 1 (DDR1) reduces collagen deposition and modulates fibroblast-specific cytokine release within the breast microenvironment

  Daniel E.C. Fein, et al., bioRxiv - Cell Biology 2023

  Quote: ... CD8-BV711 (BioLegend 100747; 1:800), CD4-APC-Cy7 (BioLegend 100413 ...
• Anti-PD-1-iRGD Peptide Conjugate Boosts Antitumor Efficacy via Engagement Augmentation and Penetration Enhancement of T cells

  Yunfeng Pan, et al., bioRxiv - Immunology 2023

  Quote: ... cell suspensions were stained with Zombie AquTM Fixable Viability Kit (#423101, Biolegend) and anti-CD45 FITC (#157607 ...
• Transkingdom mechanism of MAMP generation by chitotriosidase (CHIT1) feeds oligomeric chitin from fungal pathogens and allergens into TLR2-mediated innate immune sensing

  Tzu-Hsuan Chang, et al., bioRxiv - Immunology 2023

  Quote: Appropriate ELISA kits were used to quantify human or murine IL-6 and/or TNF concentrations according to manufacturer’s recommendations (Biolegend). The 450 nm absorbance was measured by a SpectraMax® plate reader.
• Hypoxic memory of tumor intrinsic type I interferon suppression promotes breast cancer metastasis

  Oihana Iriondo, et al., bioRxiv - Cancer Biology 2023

  Quote: ... for 15 minutes and incubated with the human-specific anti-CD298-APC antibody (Biolegend, 341706) for 30 minutes ...
• Association of NOTCH3 with Elastic Fiber Dispersion in the Infrarenal Abdominal Aorta of Cynomolgus Monkeys

  Sohei Ito, et al., bioRxiv - Pathology 2023

  Quote: ... mouse anti human CD68 antibody (0.5 µg/mL, 916104, BioLegend), or rabbit NOTCH3 antibody (1 µg ...
• GSK3α/β restrains IFNγ-inducible costimulatory molecule expression in alveolar macrophages, limiting CD4⁺ T cell activation

  Laurisa M. Ankley, et al., bioRxiv - Immunology 2023

  Quote: ... CD11a-PE (Biolegend, Cat no. 153103) (all diluted 1:400 in PBS) ...
• GSK3α/β restrains IFNγ-inducible costimulatory molecule expression in alveolar macrophages, limiting CD4⁺ T cell activation

  Laurisa M. Ankley, et al., bioRxiv - Immunology 2023

  Quote: ... and stained with MHCII-FITC (BioLegend, Cat no. 107606), CD40-APC (BioLegend ...
• GSK3α/β restrains IFNγ-inducible costimulatory molecule expression in alveolar macrophages, limiting CD4⁺ T cell activation

  Laurisa M. Ankley, et al., bioRxiv - Immunology 2023

  Quote: ... or IFNβ (BioLegend, Cat no. 581304), recombinant cytokines were added at 20 ng/mL for 24 hours ...
• Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

  Audrey Lee, et al., bioRxiv - Immunology 2023

  Quote: ... CD8α (1:200 dilution; clone 53-6.7, BioLegend), CD4 (1:200 dilution ...
• Comparative Analysis of Methods to Reduce Activation Signature Gene Expression in PBMCs

  Lovatiana Andriamboavonjy, et al., bioRxiv - Immunology 2023

  Quote: ... RBC lysis buffer (BioLegend, cat#420301) was added for 5 minutes on ice before washing the buffy coat ...
• NK cell-induced damage to P.falciparum-infected erythrocytes requires ligand-specific recognition and releases parasitophorous vacuoles that are phagocytosed by monocytes in the presence of immune IgG

  Padmapriya Sekar, et al., bioRxiv - Immunology 2023

  Quote: ... clone M1/70 (BioLegend, #101284); CD18 ...
• Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

  Audrey Lee, et al., bioRxiv - Immunology 2023

  Quote: ... and CD45 (1:200 dilution; clone 30-F11, BioLegend). Cells were incubated for 20 min at 4°C and washed 2x with PBS + 2% FBS + 1 mM EDTA ...
• Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

  Audrey Lee, et al., bioRxiv - Immunology 2023

  Quote: ... anti-TNF-α (1:100 dilution; clone MP6-XT22, BioLegend).