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8501 - 8550 of 10000+ citations since 2019

• Depletion of alloreactive B cells by chimeric alloantigen receptor T cells with drug resistance to prevent antibody-mediated rejection in solid organ transplantation

  Anna C. Dragon, et al., bioRxiv - Immunology 2023

  Quote: ... 7-AAD (BioLegend or BD) was used to discriminate dead CTV+ cells via flow cytometry ...
• MERTK Coordinates Efferocytosis by Regulating Integrin Localization and Activation

  Brandon H Dickson, et al., bioRxiv - Immunology 2023

  Quote: ... FITC anti-human CD18 antibody and FITC anti-human CD11c antibody (clone Bul15) were purchased from BioLegend (San Diego, USA). Rabbit anti-human MERTK antibody (D21F11 ...
• Injectable butyrate-prodrug micelles induce long-acting immune modulation and suppress autoimmune arthritis in mice

  Shijie Cao, et al., bioRxiv - Bioengineering 2023

  Quote: ... GM-CSF (20 ng/mL; recombinant Mouse GM-CSF (carrier-free) from BioLegend) ...
• Expression of a STING Gain-of-function Mutation in Endothelial Cells Initiates Lymphocytic Infiltration of the Lungs

  Kevin MingJie Gao, et al., bioRxiv - Immunology 2023

  Quote: ... Samples were fixed using Fluorofix buffer (Biolegend #422101). Intracellular staining was performed using a BD Biosciences Fixation/Permeabilization kit (BD Biosciences #554714 ...
• Soluble CTLA-4 mainly produced by Treg cells inhibits type 1 inflammation without hindering type 2 immunity to allow for inflammation resolution

  Motonao Osaki, Shimon Sakaguchi, bioRxiv - Immunology 2023

  Quote: ... anti- mCD45 (30-F11, BioLegend, 103140), anti-mCD45.1 (A20 ...
• Soluble CTLA-4 mainly produced by Treg cells inhibits type 1 inflammation without hindering type 2 immunity to allow for inflammation resolution

  Motonao Osaki, Shimon Sakaguchi, bioRxiv - Immunology 2023

  Quote: ... anti-mIL-4 (11B11, BD, 557728, or BioLegend, 504119), anti-m/hIL-5 (TRFK5 ...
• Soluble CTLA-4 mainly produced by Treg cells inhibits type 1 inflammation without hindering type 2 immunity to allow for inflammation resolution

  Motonao Osaki, Shimon Sakaguchi, bioRxiv - Immunology 2023

  Quote: ... 10 μg/mL anti-IL-4 mAb (11B11, BioLegend, 504122); Th2 condition ...
• Soluble CTLA-4 mainly produced by Treg cells inhibits type 1 inflammation without hindering type 2 immunity to allow for inflammation resolution

  Motonao Osaki, Shimon Sakaguchi, bioRxiv - Immunology 2023

  Quote: ... and stained with anti-Keratin 14 antibody (Poly9060, Chicken polyclonal IgY, 1:500, BioLegend), followed by anti-Chicken IgY Cross-Adsorbed Secondary Antibody conjugated with Alexa Fluor Plus 488 (Goat polyclonal IgG ...
• Multi-organ single-cell RNA-sequencing reveals early hyperglycaemia responses that converge on fibroblast dysregulation

  Adam T. Braithwaite, et al., bioRxiv - Cell Biology 2023

  Quote: ... cell suspensions were resuspended in PBS containing 2 % BSA and a viability dye (Zombie Red, Biolegend) for discrimination between live and dead cells ...
• ISWI1 complex proteins facilitate developmental genome editing in Paramecium

  Aditi Singh, et al., bioRxiv - Molecular Biology 2023

  Quote: ... and mouse anti-His (1:2500, 362601, BioLegend) were diluted in 5% BSA in 1× PBS + 0.2% Tween20 for blotting ...
• Heterogeneity of radial spokes structural components and associated enzymes in Tetrahymena cilia

  Marta Bicka, et al., bioRxiv - Cell Biology 2023

  Quote: ... monoclonal anti-HA antibody (BioLegend, San Diego, CA, USA) diluted 1:300 and 6-11 B1 (Sigma-Aldrich) ...
• Burkholderia pseudomallei BicA protein promotes pathogenicity in macrophages by regulating Invasion, Intracellular Survival, and Virulence

  Jacob L Stockton, et al., bioRxiv - Microbiology 2023

  Quote: ... detached using Accutase cell detachment solution (Biolegend) and seeded into 12 or 24 well plates (Corning) ...
• Burkholderia pseudomallei BicA protein promotes pathogenicity in macrophages by regulating Invasion, Intracellular Survival, and Virulence

  Jacob L Stockton, et al., bioRxiv - Microbiology 2023

  Quote: ... and 25 ng/mL M-CSF (Biolegend). Cells were incubated at 37°C with 5% CO2 for 5 days with media changes on days 3 and 5 ...
• Burkholderia pseudomallei BicA protein promotes pathogenicity in macrophages by regulating Invasion, Intracellular Survival, and Virulence

  Jacob L Stockton, et al., bioRxiv - Microbiology 2023

  Quote: ... The resulting adherent cells were detached from the petri dishes using Accutase cell detachment solution (Biolegend) and seeded into 12 or 24 well plates (Corning ...
• The zinc transporter Slc30a1 in macrophages plays a protective role against Salmonella infection

  Pinanong Na-Phatthalung, et al., bioRxiv - Immunology 2023

  Quote: ... The following fluorochrome-conjugated antibodies were used in this study (all from BioLegend): CD11b-Pacific Blue (clone M1/70 ...
• Functionally and metabolically divergent melanoma-associated macrophages originate from common bone-marrow precursors

  Gabriela A. Pizzurro, et al., bioRxiv - Immunology 2023

  Quote: ... CD24 BV605 (M1/69, 101827), CD103 BV711 (2E7, 121435), MHCII APC-Cy7 (M5/114.15.2, 107628), CD206 APC (C068C2, 141708) from Biolegend, Live/Dead eFluor506 (65-0866-18) ...
• Long-term cytotoxic NK cells with broad anti-tumour capacity proliferate selectively, without exhaustion, after BCG priming and extremely low doses of cytokines

  María-José Felgueres, et al., bioRxiv - Immunology 2023

  Quote: ... and stained with IFNγ-PE (Biolegend).
• Vγ9+Vδ2+Tcell-mediated purging of Listeria monocytogenes-infected epithelial cells requires butyrophilin 3A genes

  Katrin Fischer, et al., bioRxiv - Immunology 2023

  Quote: ... and γ9 TCR (Biolegend, Cat.No. B235686) or δ2 TCR staining (BD ...
• Long-term cytotoxic NK cells with broad anti-tumour capacity proliferate selectively, without exhaustion, after BCG priming and extremely low doses of cytokines

  María-José Felgueres, et al., bioRxiv - Immunology 2023

  Quote: ... PD1 (CD279)-APC (Biolegend); CD56-PC5 and NKG2A-APC (Beckman Coulter) ...
• Vγ9+Vδ2+Tcell-mediated purging of Listeria monocytogenes-infected epithelial cells requires butyrophilin 3A genes

  Katrin Fischer, et al., bioRxiv - Immunology 2023

  Quote: ... Quality and purity of isolated cells was estimated by flow cytometry after surface staining for CD3 (Biolegend, Cat.No. 300428), and γ9 TCR (Biolegend ...
• Myeloid-derived suppressor cells exacerbate poly(I:C)-induced lung inflammation in mice with renal injury and older mice

  Zhiqi Xie, et al., bioRxiv - Immunology 2023

  Quote: ... blocked with TruStain fcX (anti-mouse CD16/32) antibodies (BioLegend, CA, USA) for 5 min ...
• Engineered display of ganglioside-sugars on protein elicits a clonally and structurally constrained B cell response

  Lachlan P. Deimel, et al., bioRxiv - Immunology 2023

  Quote: ... and anti-mouse CD8-AF700 (1:200, RPA-T8, Biolegend). Following treatment with fixation and permeabilization buffers (Biolgend) ...
• Engineered display of ganglioside-sugars on protein elicits a clonally and structurally constrained B cell response

  Lachlan P. Deimel, et al., bioRxiv - Immunology 2023

  Quote: ... anti-mouse CD4-PE (1:200, RM4-5, Biolegend), anti-mouse CD8-PE (1:200 ...
• Engineered display of ganglioside-sugars on protein elicits a clonally and structurally constrained B cell response

  Lachlan P. Deimel, et al., bioRxiv - Immunology 2023

  Quote: ... 5 μg/mL brefeldin A (Biolegend) was added to all wells to suspend ER–Golgi trafficking and block cytokine secretion ...
• Engineered display of ganglioside-sugars on protein elicits a clonally and structurally constrained B cell response

  Lachlan P. Deimel, et al., bioRxiv - Immunology 2023

  Quote: ... The following surface stain cocktail was prepared: anti-mouse F4/80-PE (1:200, BM8, Biolegend), anti-mouse Gr-1 (1:200 ...
• Engineered display of ganglioside-sugars on protein elicits a clonally and structurally constrained B cell response

  Lachlan P. Deimel, et al., bioRxiv - Immunology 2023

  Quote: ... anti-mouse IgD-AF700 (1:200, 11-26c.2a, Biolegend), anti-mouse IgM-PE/Cy7 (1:200 ...
• Engineered display of ganglioside-sugars on protein elicits a clonally and structurally constrained B cell response

  Lachlan P. Deimel, et al., bioRxiv - Immunology 2023

  Quote: ... anti-mouse CD4-APC (1:200, RM4-5, Biolegend) and anti-mouse CD8-AF700 (1:200 ...
• Engineered display of ganglioside-sugars on protein elicits a clonally and structurally constrained B cell response

  Lachlan P. Deimel, et al., bioRxiv - Immunology 2023

  Quote: ... anti-mouse CD8-PE (1:200, RPA-T8, Biolegend), anti-mouse B220-eFluor450 (1:100 ...
• Engineered display of ganglioside-sugars on protein elicits a clonally and structurally constrained B cell response

  Lachlan P. Deimel, et al., bioRxiv - Immunology 2023

  Quote: ... Cells were subsequently washed with PBS with ice cold 2mM EDTA and stained with TruStain FcX Plus (Biolegend) and LIVE/DEAD Fixable Blue on ice for 30 minutes ...
• Engineered display of ganglioside-sugars on protein elicits a clonally and structurally constrained B cell response

  Lachlan P. Deimel, et al., bioRxiv - Immunology 2023

  Quote: ... Surface markers were subsequently stained on ice for 20 minutes: anti-mouse CD3-PE (1:200, 17A2, Biolegend), anti-mouse CD4-APC (1:200 ...
• Myeloid-derived suppressor cells exacerbate poly(I:C)-induced lung inflammation in mice with renal injury and older mice

  Zhiqi Xie, et al., bioRxiv - Immunology 2023

  Quote: ... a 7-amino actinomycin D viability staining solution (BioLegend) was added to each sample to stain dead cells ...
• Mycobacterium tuberculosis resides in lysosome-poor monocyte-derived lung cells during chronic infection

  Weihao Zheng, et al., bioRxiv - Immunology 2023

  Quote: ... CD44-PE/Cy7 (BioLegend, 103030), CD154-APC (BioLegend ...
• Differentiation latency and dormancy signatures define fetal liver HSCs at single cell resolution

  Takashi Ishida, et al., bioRxiv - Developmental Biology 2023

  Quote: ... CD150 biotin (clone TC15-12F12.2, Biolegend, RRID: AB_345278), Streptavidin APC-eFluor780(eBioscience ...
• Differentiation latency and dormancy signatures define fetal liver HSCs at single cell resolution

  Takashi Ishida, et al., bioRxiv - Developmental Biology 2023

  Quote: ... F4/80 FITC (clone BM8, Biolegend, RRID: AB_893502), CD201/EPCR PE (clone eBio1560 ...
• The receptor binding domain of SARS-CoV-2 spike protein fused with the type IIb E. coli heat-labile enterotoxin A subunit as an intranasal booster after mRNA vaccination

  He-Chin Hsieh, et al., bioRxiv - Microbiology 2023

  Quote: ... the plates were incubated with TMB (3,3’,5,5’-Tetramethylbenzidine) (BioLegend) substrate for 15 minutes ...
• PARP12 is required to repress the replication of a Mac1 mutant coronavirus in a cell and tissue specific manner

  Catherine M. Kerr, et al., bioRxiv - Microbiology 2023

  Quote: ... clone RA3-6B2)PDCA-1/CD317 (Pacific Blue, Biolegend, 1:200, clone 129C1), F4/80 (Pacific Orange ...
• PARP12 is required to repress the replication of a Mac1 mutant coronavirus in a cell and tissue specific manner

  Catherine M. Kerr, et al., bioRxiv - Microbiology 2023

  Quote: ... Ly6C (BV785, Biolegend, 1:300, clone HK1.4), Ly6G (PE-efluor610 ...
• PARP12 is required to repress the replication of a Mac1 mutant coronavirus in a cell and tissue specific manner

  Catherine M. Kerr, et al., bioRxiv - Microbiology 2023

  Quote: ... CD4 (BV605, Biolegend, 1:200, clone GK1.5),CD8a (APC-Cy7 ...
• Border-forming wound repair astrocytes

  Timothy M. O’Shea, et al., bioRxiv - Neuroscience 2023

  Quote: ... IP of HA-positive ribosomes was performed by incubating with Anti-HA.11 Epitope Tag Antibody (Biolegend, Cat# 901515) for 4 hours in a microcentrifuge tube on a microtube rotator kept at 4°C ...
• Discovering host protein interactions specific for SARS-CoV-2 RNA genome

  Roberto Giambruno, et al., bioRxiv - Molecular Biology 2023

  Quote: ... clone 16B12 from Biolegend (Cat #901501). Anti-Flag-M2 (Merck ...
• The focal adhesion protein talin is a mechanically-gated A-kinase anchoring protein (AKAP)

  Mingu Kang, et al., bioRxiv - Cell Biology 2023

  Quote: ... membranes were incubated with V5-HRP-containing solution (BioLegend, 1:4000, 10% NFDM) for another 12 h ...
• IL-17 signalling is critical for controlling subcutaneous adipose tissue dynamics and parasite burden during chronic Trypanosoma brucei infection

  Matthew C. Sinton, et al., bioRxiv - Immunology 2023

  Quote: ... and Brefeldin A (BioLegend) for 3 h at 37 °C and 5% CO2 ...
• IL-17 signalling is critical for controlling subcutaneous adipose tissue dynamics and parasite burden during chronic Trypanosoma brucei infection

  Matthew C. Sinton, et al., bioRxiv - Immunology 2023

  Quote: ... The CD16/CD32 receptors were then blocked by incubating with a 1/50 dilution of TruStain FcX™ (BioLegend) in PBS at room temperature for 15 min ...
• A bacterial microbiome is dispensable for the induction of CD8 T cell exhaustion

  Miriam Kuhlmann, et al., bioRxiv - Immunology 2023

  Quote: ... and anti-CD8a (53-6.7) was obtained from Biolegend. Cells were fixed in PBS containing 2% formaldehyde for 15 min ...
• IL-17 signalling is critical for controlling subcutaneous adipose tissue dynamics and parasite burden during chronic Trypanosoma brucei infection

  Matthew C. Sinton, et al., bioRxiv - Immunology 2023

  Quote: ... Cells were activated for 3 h in a round-bottom 96-well plate using Cell Activation Cocktail (with Brefeldin A) (BioLegend). Plates were then centrifuged at 300 x g for 5 min and the pellets resuspended in 50 µL of Cell-ID™ Cisplatin-195Pt viability reagent (Standard BioTools) ...
• A bacterial microbiome is dispensable for the induction of CD8 T cell exhaustion

  Miriam Kuhlmann, et al., bioRxiv - Immunology 2023

  Quote: ... and dead cells were stained using the Zombie NIR™ Fixable Viability Kit (Biolegend). Cells were stained with APC-conjugated MHC class I tetramers of H-2Db complexed with LCMV gp33-41 (TCMetrix ...
• Multiple Receptor Tyrosine Kinases Regulate Dengue Infection of Hepatocytes

  Natasha M. Bourgeois, et al., bioRxiv - Systems Biology 2023

  Quote: ... were used at a concentration of 1:100 then stained with anti-rabbit-PE or anti-mouse Pacific Blue secondary antibody (BioLegend® #406421, Life Technologies #P10993) for flow cytometry.
• Mapping the epitopes of the Human Leukocyte Antigen E antibodies 3D12 and 4D12

  Simon Brackenridge, et al., bioRxiv - Immunology 2023

  Quote: ... and 8 clones that were negative by surface staining with W6/32 (BioLegend) grown out ...
• The Regulator FleQ Post-Transcriptionally Regulates the Production of RTX Adhesins by Pseudomonas fluorescens

  Alexander B. Pastora, George A. O’Toole, bioRxiv - Microbiology 2023

  Quote: ... for 1 hour at 25°C and then transferred to TBST supplemented with purified anti-HA antibody clone 16B12 (Biolegend) at a 1:2000 dilution for 1h at 25°C ...
• Cell specificity of adeno-associated virus (AAV) serotypes in human cortical organoids

  Morgan M. Stanton, et al., bioRxiv - Neuroscience 2023

  Quote: ... Cells were centrifuged at 1000 rpm and resuspended in flow buffer containing the primary antibodies: rat GFP (Biolegend, 338001, 1:200), guinea pig MAP2 (Synaptic Systems ...