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• Single-cell multi-omic analysis of thymocyte development reveals drivers of CD4/CD8 lineage commitment

  Zoë Steier, et al., bioRxiv - Immunology 2023

  Quote: We prepared a panel containing 111 antibodies (TotalSeq-A mouse antibody panel 1, BioLegend, 900003217), which are enumerated in Table S1 ...
• Single-cell multi-omic analysis of thymocyte development reveals drivers of CD4/CD8 lineage commitment

  Zoë Steier, et al., bioRxiv - Immunology 2023

  Quote: ... or Zombie UV Fixable Viability Kit (Biolegend). Samples were blocked with anti-CD16/32 (2.4G2 ...
• Single-cell multi-omic analysis of thymocyte development reveals drivers of CD4/CD8 lineage commitment

  Zoë Steier, et al., bioRxiv - Immunology 2023

  Quote: ... Zombie NIR Fixable Viability Kit (Biolegend). Samples were blocked with anti-CD16/32 (2.4G2 ...
• ALK signalling primes the DNA damage response sensitizing ALK-driven neuroblastoma to therapeutic ATR inhibition

  Marcus Borenäs, et al., bioRxiv - Cancer Biology 2023

  Quote: ... CD8 (BV711; 100747) (Biolegend, San Diego, CA); TCRαβ (PE-CF594 ...
• Atypical B cells mediate poor response to Bacillus Calmette Guérin immunotherapy in non-muscle invasive bladder cancer

  Priyanka Yolmo, et al., bioRxiv - Cancer Biology 2023

  Quote: Plasma Ig were analysed using the LEGENDplex Mouse Immunoglobulin Isotyping panel (6-plex) by flow cytometry (Cat #740493, BioLegend, CA, USA). Plasma sample and standards was prepared using assay buffer as per the manufacturer’s instruction ...
• Enhancing anti-EGFRvIII CAR T cell therapy against glioblastoma with a paracrine SIRPγ-derived CD47 blocker

  Tomás A. Martins, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and (2) anti-NESTIN (clone 10C2; #656802, BioLegend, USA) and anti-EGFRvIII (clone RM419 ...
• Enhancing anti-EGFRvIII CAR T cell therapy against glioblastoma with a paracrine SIRPγ-derived CD47 blocker

  Tomás A. Martins, et al., bioRxiv - Cancer Biology 2023

  Quote: ... a fixation/permeabilization step was performed by incubating the cells for 20 min at RT using a Cyto-Fast Fix/Perm Buffer set (#426803, BioLegend, USA). Intracellular antibody staining was then performed for 25 min at RT ...
• Enhancing anti-EGFRvIII CAR T cell therapy against glioblastoma with a paracrine SIRPγ-derived CD47 blocker

  Tomás A. Martins, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and mouse TruStain FcX (#101320, BioLegend, USA) for 10 min ...
• Epigenomic Analyses Identify FOXM1 As a Key Regulator of Anti-Tumor Immune Response in Esophageal Adenocarcinoma

  Benjamin Ziman, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Cells were then stained with the following antibodies: Zombie Violet (BioLegend, #423113), CD3 conjugated PerCP/Cyanine (BioLegend ...
• WFIKKN2 is a bifunctional axon guidance cue that signals through divergent DCC family receptors

  Kelsey R. Nickerson, et al., bioRxiv - Developmental Biology 2023

  Quote: ... rabbit polyclonal antibodies against TuJ1 (Biolegend, 1:500), Peripherin (Millipore ...
• DNA Origami Tension Sensors (DOTS) to study T cell receptor mechanics at membrane junctions

  Yuesong Hu, et al., bioRxiv - Biophysics 2023

  Quote: ... biotinylated anti-mouse CD3e (Cat# 100304) and FITC anti-mouse TCRb antibody (Cat# 109206) were purchased from BioLegend (San Diego, CA). Azide-PEG4-NHS ester (Cat# AZ103-100 ...
• Single-cell multi-omic analysis of thymocyte development reveals drivers of CD4/CD8 lineage commitment

  Zoë Steier, et al., bioRxiv - Immunology 2023

  Quote: ... Propidium Iodine (Biolegend), Ghost Violet 510 (Tonbo) ...
• Injectable butyrate-prodrug micelles induce long-acting immune modulation and suppress autoimmune arthritis in mice

  Shijie Cao, et al., bioRxiv - Bioengineering 2023

  Quote: ... CD40 (PerCP-Cy5.5, Cat#124623, BioLegend), I-A/I-E (FITC ...
• Injectable butyrate-prodrug micelles induce long-acting immune modulation and suppress autoimmune arthritis in mice

  Shijie Cao, et al., bioRxiv - Bioengineering 2023

  Quote: ... CD169 (BV605, Cat#142413, BioLegend), Foxp3 (AF488 ...
• Leukemia circulation kinetics revealed through blood exchange method

  Alex B. Miller, et al., bioRxiv - Cancer Biology 2023

  Quote: ... 20µg (100µL) of recombinant mouse E-selectin (BioLegend: 755504) was injected retro-orbitally 20 minutes prior to blood exchange.
• Pathogenic Mutations in the C2A Domain of Dysferlin form Amyloid that Activates the Inflammasome

  Isaac L. Scott, et al., bioRxiv - Biophysics 2023

  Quote: ... TMB and Streptavidin conjugated to horseradish peroxidase (HRP) (Catalog 405103, Lot B167931) were from Biolegend (San Diego, CA). Pierce lactate dehydrogenase (LDH ...
• Bile acids mediate liver-bone marrow crosstalk

  Daniele Vitale, et al., bioRxiv - Immunology 2023

  Quote: ... FITC Anti mouse/rat CD29 (Biolegend, Cat. 102205), PE/Cy7 anti-mouse CD105 (Biolegend ...
• Bile acids mediate liver-bone marrow crosstalk

  Daniele Vitale, et al., bioRxiv - Immunology 2023

  Quote: ... Brilliant Violet 711 anti-mouse Ly-6A/E (Sca-1) (Biolegend, Cat. 108131), APC/Cy7 anti-mouse CD48 (Biolegend ...
• EZH2 inhibition promotes tumor immunogenicity in lung squamous cell carcinomas

  Tanner J. DuCote, et al., bioRxiv - Cancer Biology 2023

  Quote: ... NGFR-PECy7 (BioLegend, #345110), CD49f-FITC (Invitrogen ...
• EZH2 inhibition promotes tumor immunogenicity in lung squamous cell carcinomas

  Tanner J. DuCote, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Antibodies used for mouse studies: I-A/I-E-PerCP-Cy5.5 (BioLegend #107626), H-2Kd/H-2Dd-Alexa Fluor® 647 (BioLegend #114712) ...
• EZH2 inhibition promotes tumor immunogenicity in lung squamous cell carcinomas

  Tanner J. DuCote, et al., bioRxiv - Cancer Biology 2023

  Quote: ... CD11b-PECy7 (BioLegend # 101216) with the live-dead stain Zombie UV™ (BioLegend 423107) ...
• EZH2 inhibition promotes tumor immunogenicity in lung squamous cell carcinomas

  Tanner J. DuCote, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-CD45-FITC (BioLegend #103108), anti-CD3-BB700 (BioLegend #100328) ...
• EZH2 inhibition promotes tumor immunogenicity in lung squamous cell carcinomas

  Tanner J. DuCote, et al., bioRxiv - Cancer Biology 2023

  Quote: ... F4/80-PECF594 (BioLegend #123146), CD11b-PECy7 (BioLegend # 101216 ...
• EZH2 inhibition promotes tumor immunogenicity in lung squamous cell carcinomas

  Tanner J. DuCote, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-CD4-BV786 (BioLegend #100552), anti-CD45-FITC (BioLegend #103108) ...
• EZH2 inhibition promotes tumor immunogenicity in lung squamous cell carcinomas

  Tanner J. DuCote, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Sca1-APCCy7 (BioLegend #108126), EpCAM-PECy7 (BioLegend #118216) ...
• EZH2 inhibition promotes tumor immunogenicity in lung squamous cell carcinomas

  Tanner J. DuCote, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Ly6G-BV786 (BioLegend #127645), F4/80-PECF594 (BioLegend #123146) ...
• Inhibition of BCR::ABL1 tyrosine kinase activity Aids in the Generation of Stable Chronic Myeloid Leukemia Induced Pluripotent Stem Cells

  Esther Sathya Bama Benjamin, et al., bioRxiv - Cancer Biology 2023

  Quote: ... the cells were stained with an APC-conjugated anti-CD34 antibody (BioLegend).
• Various Cell Types in the Bone Marrow sustain Primary B-Cell Precursor Acute Lymphoblastic Leukemia

  Mandy W. E. Smeets, et al., bioRxiv - Cancer Biology 2023

  Quote: ... which included Brilliant violet 421 anti-human CD19 (Biolegend), and Alexa Fluor 750-conjugated human ALCAM/CD166 (R&D Systems ...
• Prolonged exposure to lung-derived cytokines is associated with inflammatory activation of microglia in patients with COVID-19

  Rogan A. Grant, et al., bioRxiv - Immunology 2023

  Quote: ... Cells were then resuspended in 50µL ice-cold 1:10 human TruStain FcX (BioLegend 422302) in MACS buffer and incubated for at least 5 minutes on ice ...
• Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

  Audrey Lee, et al., bioRxiv - Immunology 2023

  Quote: ... anti-CD11c (1:400; BV421; Biolegend #117330), anti-CD86 (1:300 ...
• Activation of Autoreactive Lymphocytes in the Lung by STING Gain-of-function Mutation Radioresistant Cells

  Kevin MingJie Gao, et al., bioRxiv - Immunology 2023

  Quote: ... Absolute cell counts were determined using counting beads (Biolegend #424902). Cells were acquired on an Aurora (Cytek ...
• Regulation of BCR-mediated Ca²⁺ mobilization by MIZ1-TIMBIM4 safeguards IgG1⁺ GC B cell positive selection

  Lingling Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... PerCP/Cyanine5.5 Rat Anti-Mouse CD19 (Clone 6D5; BioLegend; Cat#115534; RRID: AB_2072925); BUV395 Rat Anti-Mouse CD19 (Clone 1D3 ...
• Regulation of BCR-mediated Ca²⁺ mobilization by MIZ1-TIMBIM4 safeguards IgG1⁺ GC B cell positive selection

  Lingling Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... BV421™ anti-mouse/human CD45R/B220 Antibody (Clone RA3-6B2; BioLegend; Cat#103251; RRID: AB_2562905) BV605 Mouse Anti-Mouse CD45.1 (Clone A20 ...
• Ex vivo quantification of anti-tumor T-cell activity upon anti-PD-1 treatment in patient-derived lung tumor-on-chip

  Irina Veith, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Anti-PD-1 antibody (Selleck, #SE-A2002-5MG,) or IgG4 isotype control (Biolegend, #403702) were added at a concentration of 10µg/ml.
• Ex vivo quantification of anti-tumor T-cell activity upon anti-PD-1 treatment in patient-derived lung tumor-on-chip

  Irina Veith, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-OX-40 (Biolegend, #350012, diluted 1:20), anti-CD137 (BD ...
• A novel miR-99b-5p-Zbp1 pathway in microglia contributes to the pathogenesis of schizophrenia

  Lalit Kaurani, et al., bioRxiv - Neuroscience 2023

  Quote: ... and CD11b FITC (Clone M1/70, Biolegend). Antibody-labeled CD45low CD11b+ microglial cells were sorted using a FACSAria 4L SORP cell sorter (Becton Dickinson ...
• A novel miR-99b-5p-Zbp1 pathway in microglia contributes to the pathogenesis of schizophrenia

  Lalit Kaurani, et al., bioRxiv - Neuroscience 2023

  Quote: ... Single cell suspension was labeled by using anti-mouse CD45 BV 421 (Clone 30-F11, Biolegend) and CD11b FITC (Clone M1/70 ...
• Selective Inhibition of Interleukin-2 Inducible T Cell Kinase (ITK) Enhances Anti-Tumor Immunity in Association with Th1-skewing, Cytotoxic T cell Activation, and Reduced T Cell Exhaustion

  Lih-Yun Hsu, et al., bioRxiv - Cancer Biology 2023

  Quote: ... CD4 (GK1.5, Biolegend), PD-1 (29F.1A12 ...
• Activated interstitial macrophages are a predominant target of viral takeover and focus of inflammation in COVID-19 initiation in human lung

  Timothy Ting-Hsuan Wu, et al., bioRxiv - Genomics 2023

  Quote: ... CD206 (Biolegend 321132), CD14 (BD 562698) ...
• Activated interstitial macrophages are a predominant target of viral takeover and focus of inflammation in COVID-19 initiation in human lung

  Timothy Ting-Hsuan Wu, et al., bioRxiv - Genomics 2023

  Quote: ... CD204 (Biolegend 371906), CD206 (Biolegend 321132) ...
• Activated interstitial macrophages are a predominant target of viral takeover and focus of inflammation in COVID-19 initiation in human lung

  Timothy Ting-Hsuan Wu, et al., bioRxiv - Genomics 2023

  Quote: ... then incubated with cell viability marker propidium iodide (PI, 1 μg/mL, Biolegend 421301). Flow cytometry was performed on a FACS Aria II (BD Biosciences).
• Single-component multilayered self-assembling protein nanoparticles displaying extracellular domains of matrix protein 2 as a pan-influenza A vaccine

  Keegan Braz Gomes, et al., bioRxiv - Microbiology 2023

  Quote: ... FITC anti-mouse CD3 antibody (BioLegend, catalog no. 100204), Alexa Fluor 700 anti-mouse CD4 antibody (BioLegend ...
• Single-component multilayered self-assembling protein nanoparticles displaying extracellular domains of matrix protein 2 as a pan-influenza A vaccine

  Keegan Braz Gomes, et al., bioRxiv - Microbiology 2023

  Quote: ... including Zombie NIR live/dead stain (BioLegend, catalog no. 423106), Brilliant Violet 510 anti-mouse/human CD45R/B220 antibody (BioLegend ...
• Sequential early-life viral infections modulate the microbiota and adaptive immune responses to systemic and mucosal vaccination

  Yuhao Li, et al., bioRxiv - Microbiology 2023

  Quote: ... and CD19 (BioLegend, 1:200, respectively), and Fixable Aqua Dead Cell Stain (Invitrogen ...
• Sequential early-life viral infections modulate the microbiota and adaptive immune responses to systemic and mucosal vaccination

  Yuhao Li, et al., bioRxiv - Microbiology 2023

  Quote: ... PD-1(Biolegend, RMP1-30, 1:200), KLRG1(Biolegend ...
• Sequential early-life viral infections modulate the microbiota and adaptive immune responses to systemic and mucosal vaccination

  Yuhao Li, et al., bioRxiv - Microbiology 2023

  Quote: ... CD103(Biolegend, M290, 1:200), PD-1(Biolegend ...
• Single-component multilayered self-assembling protein nanoparticles displaying extracellular domains of matrix protein 2 as a pan-influenza A vaccine

  Keegan Braz Gomes, et al., bioRxiv - Microbiology 2023

  Quote: ... APC/Fire 810 anti-mouse CD279 (PD-1) antibody (BioLegend, catalog no. 135251), and Brilliant Violet 605 anti-mouse CD185 (CXCR5 ...
• Single-component multilayered self-assembling protein nanoparticles displaying extracellular domains of matrix protein 2 as a pan-influenza A vaccine

  Keegan Braz Gomes, et al., bioRxiv - Microbiology 2023

  Quote: ... APC anti-mouse CD154 antibody (BioLegend, catalog no. 106510), Brilliant Violet 421 anti-mouse CD69 Antibody (BioLegend ...
• Murine leukemia virus can infect non-dividing cells

  Karen Salas-Briceno, Wenming Zhao, Susan R. Ross, bioRxiv - Microbiology 2023

  Quote: ... cells were stained with 7-AAD Viability staining solution (BioLegend), Zombie Aqua Dye (BioLegend) ...
• Identification of kinase inhibitors as potential host-directed therapies for intracellular bacteria

  Robin H.G.A. van den Biggelaar, et al., bioRxiv - Microbiology 2023

  Quote: ... CD14-PE/Cy7 (clone 63D3) and CD1a-AF647 (clone HI149) were purchased from Biolegend (San Diego, CA, USA). Mouse anti-human CD11b-BB515 (clone ICRF44 ...