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• ISWI1 complex proteins facilitate developmental genome editing in Paramecium

  Aditi Singh, et al., bioRxiv - Molecular Biology 2023

  Quote: ... and mouse anti-His (1:2500, 362601, BioLegend) were diluted in 5% BSA in 1× PBS + 0.2% Tween20 for blotting ...
• Differentiation latency and dormancy signatures define fetal liver HSCs at single cell resolution

  Takashi Ishida, et al., bioRxiv - Developmental Biology 2023

  Quote: ... ESAM FITC(clone 1GB/ESAM, Biolegend, RRID: AB_2044017), SCA1 Alexa Fluor700 (clone D7 ...
• Differentiation latency and dormancy signatures define fetal liver HSCs at single cell resolution

  Takashi Ishida, et al., bioRxiv - Developmental Biology 2023

  Quote: ... CD19 APC (clone 1D3/CD19, Biolegend, RRID: AB_2629839), CD45.2 APCeFluor780 (clone 104 ...
• Differentiation latency and dormancy signatures define fetal liver HSCs at single cell resolution

  Takashi Ishida, et al., bioRxiv - Developmental Biology 2023

  Quote: ... CD63 PE (clone NVG-2, Biolegend, RRID: AB_11203532). DAPI (Millipore ...
• A CRISPR screen of HIV dependency factors reveals CCNT1 is non-essential in T cells but required for HIV-1 reactivation from latency

  Terry L Hafer, et al., bioRxiv - Microbiology 2023

  Quote: ... An early activation marker of T cells was also monitored using PE-Conjugated CD-69 antibody (Biolegend, 310906). Analysis was performed on FlowJo software ...
• An N-terminal delivery domain defines a new class of polymorphic T6SS effectors in Enterobacterales

  Andrea Carobbi, et al., bioRxiv - Microbiology 2023

  Quote: ... or anti-RNA polymerase sigma 70 (RNAp) antibodies (BioLegend ; mouse mAb #663205) for loading and lysis control ...
• Pericardial & Mediastinal Fat-Associated Lymphoid Clusters are rapidly activated in an alkane induced model of Systemic Lupus Erythematosus

  Karolina Bentkowska, et al., bioRxiv - Immunology 2023

  Quote: A mix and match Murine anti-viral Legendplex array (Biolegend) was used to detect CXCL1 and a 6-plex murine isotyping panel was used to detect total IgM ...
• Pericardial & Mediastinal Fat-Associated Lymphoid Clusters are rapidly activated in an alkane induced model of Systemic Lupus Erythematosus

  Karolina Bentkowska, et al., bioRxiv - Immunology 2023

  Quote: Murine cells were stained to distinguish live versus dead using Zombie aqua (Biolegend), blocked with anti-murine CD16/32 (clone 2.4G2 ...
• Pericardial & Mediastinal Fat-Associated Lymphoid Clusters are rapidly activated in an alkane induced model of Systemic Lupus Erythematosus

  Karolina Bentkowska, et al., bioRxiv - Immunology 2023

  Quote: ... 50μl of 3,3 ‘,5,5 ‘-Tetramethylbenzidine (TMB; Biolegend) was added ...
• Activation of Autoreactive Lymphocytes in the Lung by STING Gain-of-function Mutation Radioresistant Cells

  Kevin MingJie Gao, et al., bioRxiv - Immunology 2023

  Quote: ... after stimulating cells for 4 hours with Brefeldin A (Biolegend #420601). Absolute cell counts were determined using counting beads (Biolegend #424902) ...
• Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

  Audrey Lee, et al., bioRxiv - Immunology 2023

  Quote: ... anti-Ly6C (1:500; BV780; BioLegend #128041), anti-Ly6G (1:400 ...
• Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

  Audrey Lee, et al., bioRxiv - Immunology 2023

  Quote: ... anti-EpCAM (1:100; Biolegend 118214). Samples were then washed twice with PBS + 2% FBS + 1 mM EDTA and fixed with BD Cytofix (#554655) ...
• Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

  Audrey Lee, et al., bioRxiv - Immunology 2023

  Quote: ... anti-Ly6G (1:400; APC-Cy7; BioLegend #127624), anti-CD19 (1:100 ...
• Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

  Audrey Lee, et al., bioRxiv - Immunology 2023

  Quote: ... anti-CD45 (1:200; BV610; BioLegend #103140), anti-CD169 (1:200 ...
• Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

  Audrey Lee, et al., bioRxiv - Immunology 2023

  Quote: ... Single cells were stained with antibodies: Zombie UVTM (1:200 dilution; BUV496; Biolegend #423107), anti-Ly6C (1:500 ...
• Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

  Audrey Lee, et al., bioRxiv - Immunology 2023

  Quote: ... anti-CD11b (1:300; BV650; BioLegend #101239), anti-CD11c (1:400 ...
• Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

  Audrey Lee, et al., bioRxiv - Immunology 2023

  Quote: ... anti-CD169 (1:200; PE-Cy7; BioLegend #142412), anti-PDCA-1 (1:200 ...
• Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

  Audrey Lee, et al., bioRxiv - Immunology 2023

  Quote: ... CD69 (1:200 dilution; clone H1.2F3; BioLegend) and CD45 (1:200 dilution ...
• Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

  Audrey Lee, et al., bioRxiv - Immunology 2023

  Quote: ... IL-2 (1:100 dilution; clone JES6-5H4, BioLegend) and anti-IL-4 (1:100 dilution ...
• Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

  Audrey Lee, et al., bioRxiv - Immunology 2023

  Quote: ... CD4 (1:200 dilution; clone RM4-5, BioLegend), CD44 (1:400 dilution ...
• Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

  Audrey Lee, et al., bioRxiv - Immunology 2023

  Quote: ... and anti-IL-4 (1:100 dilution; clone 11B11, BioLegend). Cells were then washed twice with 1x Perm buffer and fixed with BD Cytofix for 10 min at room temp ...
• Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

  Audrey Lee, et al., bioRxiv - Immunology 2023

  Quote: ... for 5 min on ice before staining with fluorochrome-conjugated antibodies in FACS staining buffer (1× PBS, 2% FBS): CD3 (1:50 dilution; clone 145-2C11, BioLegend), CD8α (1:200 dilution ...
• Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

  Audrey Lee, et al., bioRxiv - Immunology 2023

  Quote: ... CD44 (1:400 dilution; clone IM7, BioLegend), CD69 (1:200 dilution ...
• NK cell-induced damage to P.falciparum-infected erythrocytes requires ligand-specific recognition and releases parasitophorous vacuoles that are phagocytosed by monocytes in the presence of immune IgG

  Padmapriya Sekar, et al., bioRxiv - Immunology 2023

  Quote: ... clone TS1/8 (BioLegend, # 309236); CD11a ...
• Anti-PD-1-iRGD Peptide Conjugate Boosts Antitumor Efficacy via Engagement Augmentation and Penetration Enhancement of T cells

  Yunfeng Pan, et al., bioRxiv - Immunology 2023

  Quote: ... and anti-CD45 FITC (#157607, Biolegend) for 30 minutes at 4[ ...
• Anti-PD-1-iRGD Peptide Conjugate Boosts Antitumor Efficacy via Engagement Augmentation and Penetration Enhancement of T cells

  Yunfeng Pan, et al., bioRxiv - Immunology 2023

  Quote: ... anti-mouse GZMB (AF647, Biolegend, #515406), anti-IFNγ (BV570 ...
• Anti-PD-1-iRGD Peptide Conjugate Boosts Antitumor Efficacy via Engagement Augmentation and Penetration Enhancement of T cells

  Yunfeng Pan, et al., bioRxiv - Immunology 2023

  Quote: ... single-cell suspensions were stained with anti-mouse IFNγ (APC, Biolegend, #505809; Percp-Cy5.5, #505822), anti-mouse GZMB (AF647 ...
• Single-cell profiling uncovers regulatory programs of pathogenic Th2 cells in allergic asthma

  Matarr Khan, et al., bioRxiv - Immunology 2023

  Quote: ... and red blood cell lysis was performed using 1x RBC lysis buffer (BioLegend, 420301). Total cells were determined using a Coulter counter (Beckman Coulter) ...
• Altered metabolic function induced by amyloid-beta oligomers and PSEN1-mutations in iPSC-derived astrocytes

  R. J. Elsworthy, et al., bioRxiv - Neuroscience 2023

  Quote: ... and IL-8 FITC (BH0814, BioLegend) conjugated antibodies for 30 minutes ...
• CDDO-Methyl Ester Inhibits BRAF Inhibitor Resistance and Remodels the Myeloid Compartment in BRAF-mutant Melanoma

  Gretel M. Torres, et al., bioRxiv - Immunology 2023

  Quote: ... Single cell suspensions were then tagged with mouse Total-Seq anti-mouse hashtags C0306 (Brafi: Hashtag 6) and C0307 (Brafi + C-Me: Hashtag 7) (BioLegend) according to manufacturers’ protocols.
• DOCK2-deficiency causes defects in anti-viral T cell responses and poor control of herpes simplex virus infection

  Katrina L. Randall, et al., bioRxiv - Immunology 2023

  Quote: ... anti-CD8 (clone 53-6.7; BioLegend) and in some cases anti-CD62L (MEL-14 ...
• CDDO-Methyl Ester Inhibits BRAF Inhibitor Resistance and Remodels the Myeloid Compartment in BRAF-mutant Melanoma

  Gretel M. Torres, et al., bioRxiv - Immunology 2023

  Quote: ... Single-cell suspensions were stained with fluorophore-conjugated antibodies obtained from BioLegend: anti-CD206-Pe-Dazzle ...
• DOCK2-deficiency causes defects in anti-viral T cell responses and poor control of herpes simplex virus infection

  Katrina L. Randall, et al., bioRxiv - Immunology 2023

  Quote: ... and in some cases anti-CD62L (MEL-14, BioLegend) and intracellular staining with anti-GzmB (GB11 ...
• DOCK2-deficiency causes defects in anti-viral T cell responses and poor control of herpes simplex virus infection

  Katrina L. Randall, et al., bioRxiv - Immunology 2023

  Quote: ... anti-CD8 (as above) and anti-IFNγ (XMG1.2, BioLegend), stained intracellularly[17] ...
• The zinc transporter Slc30a1 in macrophages plays a protective role against Salmonella infection

  Pinanong Na-Phatthalung, et al., bioRxiv - Immunology 2023

  Quote: ... The following fluorochrome-conjugated antibodies were used in this study (all from BioLegend): CD11b-Pacific Blue (clone M1/70 ...
• Functionally and metabolically divergent melanoma-associated macrophages originate from common bone-marrow precursors

  Gabriela A. Pizzurro, et al., bioRxiv - Immunology 2023

  Quote: ... CD24 BV605 (M1/69, 101827), CD103 BV711 (2E7, 121435), MHCII APC-Cy7 (M5/114.15.2, 107628), CD206 APC (C068C2, 141708) from Biolegend, Live/Dead eFluor506 (65-0866-18) ...
• Long-term cytotoxic NK cells with broad anti-tumour capacity proliferate selectively, without exhaustion, after BCG priming and extremely low doses of cytokines

  María-José Felgueres, et al., bioRxiv - Immunology 2023

  Quote: ... and stained with IFNγ-PE (Biolegend).
• Vγ9+Vδ2+Tcell-mediated purging of Listeria monocytogenes-infected epithelial cells requires butyrophilin 3A genes

  Katrin Fischer, et al., bioRxiv - Immunology 2023

  Quote: ... and γ9 TCR (Biolegend, Cat.No. B235686) or δ2 TCR staining (BD ...
• Long-term cytotoxic NK cells with broad anti-tumour capacity proliferate selectively, without exhaustion, after BCG priming and extremely low doses of cytokines

  María-José Felgueres, et al., bioRxiv - Immunology 2023

  Quote: ... PD1 (CD279)-APC (Biolegend); CD56-PC5 and NKG2A-APC (Beckman Coulter) ...
• Vγ9+Vδ2+Tcell-mediated purging of Listeria monocytogenes-infected epithelial cells requires butyrophilin 3A genes

  Katrin Fischer, et al., bioRxiv - Immunology 2023

  Quote: ... Quality and purity of isolated cells was estimated by flow cytometry after surface staining for CD3 (Biolegend, Cat.No. 300428), and γ9 TCR (Biolegend ...
• Myeloid-derived suppressor cells exacerbate poly(I:C)-induced lung inflammation in mice with renal injury and older mice

  Zhiqi Xie, et al., bioRxiv - Immunology 2023

  Quote: ... blocked with TruStain fcX (anti-mouse CD16/32) antibodies (BioLegend, CA, USA) for 5 min ...
• Engineered display of ganglioside-sugars on protein elicits a clonally and structurally constrained B cell response

  Lachlan P. Deimel, et al., bioRxiv - Immunology 2023

  Quote: ... and anti-mouse CD8-AF700 (1:200, RPA-T8, Biolegend). Following treatment with fixation and permeabilization buffers (Biolgend) ...
• Engineered display of ganglioside-sugars on protein elicits a clonally and structurally constrained B cell response

  Lachlan P. Deimel, et al., bioRxiv - Immunology 2023

  Quote: ... anti-mouse CD4-PE (1:200, RM4-5, Biolegend), anti-mouse CD8-PE (1:200 ...
• Engineered display of ganglioside-sugars on protein elicits a clonally and structurally constrained B cell response

  Lachlan P. Deimel, et al., bioRxiv - Immunology 2023

  Quote: ... 5 μg/mL brefeldin A (Biolegend) was added to all wells to suspend ER–Golgi trafficking and block cytokine secretion ...
• Engineered display of ganglioside-sugars on protein elicits a clonally and structurally constrained B cell response

  Lachlan P. Deimel, et al., bioRxiv - Immunology 2023

  Quote: ... The following surface stain cocktail was prepared: anti-mouse F4/80-PE (1:200, BM8, Biolegend), anti-mouse Gr-1 (1:200 ...
• Engineered display of ganglioside-sugars on protein elicits a clonally and structurally constrained B cell response

  Lachlan P. Deimel, et al., bioRxiv - Immunology 2023

  Quote: ... anti-mouse IgD-AF700 (1:200, 11-26c.2a, Biolegend), anti-mouse IgM-PE/Cy7 (1:200 ...
• Engineered display of ganglioside-sugars on protein elicits a clonally and structurally constrained B cell response

  Lachlan P. Deimel, et al., bioRxiv - Immunology 2023

  Quote: ... anti-mouse CD4-APC (1:200, RM4-5, Biolegend) and anti-mouse CD8-AF700 (1:200 ...
• Engineered display of ganglioside-sugars on protein elicits a clonally and structurally constrained B cell response

  Lachlan P. Deimel, et al., bioRxiv - Immunology 2023

  Quote: ... anti-mouse CD8-PE (1:200, RPA-T8, Biolegend), anti-mouse B220-eFluor450 (1:100 ...
• Engineered display of ganglioside-sugars on protein elicits a clonally and structurally constrained B cell response

  Lachlan P. Deimel, et al., bioRxiv - Immunology 2023

  Quote: ... Cells were subsequently washed with PBS with ice cold 2mM EDTA and stained with TruStain FcX Plus (Biolegend) and LIVE/DEAD Fixable Blue on ice for 30 minutes ...
• Engineered display of ganglioside-sugars on protein elicits a clonally and structurally constrained B cell response

  Lachlan P. Deimel, et al., bioRxiv - Immunology 2023

  Quote: ... Surface markers were subsequently stained on ice for 20 minutes: anti-mouse CD3-PE (1:200, 17A2, Biolegend), anti-mouse CD4-APC (1:200 ...