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Citations for BioLegend :
8101 - 8150 of 8821 citations for 1 6 ANHYDRO β D GLUCOSE 2 3 4 TRI O ACETATE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... washed with PBS and stained for ASC using a primary antibody against the CARD domain (Biolegend; 1:1000 dilution) followed by an Alexa Fluor conjugated secondary antibody (Life technologies ...
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bioRxiv - Immunology 2021Quote: ... and stained with 1 ug/test cell-hashing antibody (TotalSeq C0251, C0254, C0256, C0260, clones LNH-95, 2M2, BioLegend) for 30 minutes at 4C ...
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bioRxiv - Immunology 2021Quote: ... The cells were washed with PBS/BSA/EDTA and stained either with anti-IgM PE (dilution 1:100, Biolegend) and anti-IgG Alexa FluorTM 647 (dilution 1:600 ...
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bioRxiv - Systems Biology 2021Quote: ... After spinning them down the cells were incubated overnight at 4C with PerCP/Cy5.5 anti-BrdU (1:30 dilution) (BioLegend) in a buffer containing 0.5% Tween 20 / 1% BSA/PBS and RNase (0.8 mg/ml) ...
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bioRxiv - Immunology 2021Quote: ... the cells were fixed for 1 h at RT using the True-Nuclear transcription Factor Buffer Set (BioLegend, 424401). Following the fixation ...
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bioRxiv - Bioengineering 2020Quote: ... Levels of TNF-α mRNA and protein were detected by qPCR (Table 1) and ELISA (BioLegend, San Diego, CA), respectively.
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bioRxiv - Neuroscience 2021Quote: ... for 15 min and cells were stained with fluorophore-conjugated antibodies: CD11b BV605 (1:200, clone M1/70, Biolegend), CD45 PerCP-Cy5.5 (1:200 ...
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bioRxiv - Cancer Biology 2020Quote: Single cell suspension obtained by trypsin treatment was washed and incubated with an anti-mouse CD326 (EpCAM) AlexaFluor 647 antibody (1:2000, clone G8.8, Biolegend). DAPI (10 μg/mL ...
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bioRxiv - Biochemistry 2021Quote: The following antibodies were used in this study: Anti-ZBP1 clone RonoB19 (WB 1:500, ref. 651602, Rat, BioLegend), anti-TIAR (C-18 ...
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bioRxiv - Biochemistry 2020Quote: ... Tetramers were prepared by mixing 1 or 5 pmol of biotinylated protein monomer with 0.25 to 1.25 pmol of streptavidin-PE (Biolegend #405245) respectively and incubating for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2021Quote: Whole blood or washed platelets were labelled with anti-human BV421 CD62p (P-selectin; 1/100, AK4, BioLegend, 304926), BV421 CD42b(1/300 ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated in 5% dried non-fat milk powder dissolved in TBS-Tween 0,1% (TBST) for 1 h at room temperature (RT) and subsequently incubated with primary anti-HA antibody (Biolegend) followed by incubation by secondary goat HRP-conjugated anti-mouse IgG (Promega) ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated with Calbryte 630TM (2μM; Stratech) for 30 minutes at 37°C followed by staining with CD42b-BV421 (1:100; Biolegend) for 15 minutes at room temperature and supplementation with 2mM CaCl2 ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated with 100 μl/well of the fixable dead cell viability dye Zombie Aqua (1:800, Biolegend) diluted in PBS for 30 min on ice ...
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bioRxiv - Cell Biology 2021Quote: 1×106 (for immunoblot) or 1.8×107 (for immunoprecipitation) Jurkat Cells were incubated with anti-CD3 (1 μg/ml, OKT3, 317325, BioLegend) and anti-CD28 (1 μg/ml ...
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bioRxiv - Genomics 2020Quote: ... Cells were then stained with a panel of 9 further DNA-oligonucleotide conjugated antibodies (0.33 μg/ml anti-CD71: BioLegend 113802 conjugated with ADT9 [CGAAGAAGGAGT], 1 μg/ml anti-CD41: BioLegend 133919 conjugated with ADT3 [TGTCCGGCAATA] ...
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bioRxiv - Immunology 2022Quote: ... neutrophils were incubated with autologous activated platelets (1:10 ratio) (65) for 2.5h and human recombinant CCL28 (50 nM) (BioLegend), the CCR3 antagonist SB328437 (10 mM ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated with PE-streptavidin (BioLegend, 405204), SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... primary antibody 4G8 was incubated overnight at a 1:1000 dilution at room temperature (RT) (Biolegend, San Diego, CA). HRP-linked secondary goat anti-mouse antibody at a 1:1000 dilution (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... The cell suspension was stained using allophycocyanin (APC)/Cy7-conjugated rat anti-mouse CD45 antibody (1:200, BioLegend, #101319), PE-conjugated rat anti-mouse EpCam antibody (1:500 ...
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bioRxiv - Immunology 2022Quote: ... and the cells were resuspended in 200 μL of PBS supplemented with 1% FBS and containing 2.5 μL of Alexa Fluor 647-conjugated anti-human CD127antibody (BioLegend) or Alexa Fluor 647-conjugated mouse IgG1 k isotype control (BioLegend) ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: Both FLT3L and GM-CSF BMDCs were stained with 1:1000 Zombie Green Fixable Viability Kit (BioLegend, Cat# 423111) and were blocked with anti-mouse CD16/32 (BioLegend ...
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bioRxiv - Microbiology 2022Quote: The HA epitope was detected with mouse monoclonal antibody (mAb) HA.11 (diluted 1:1000) (BioLegend, item no. 901515) or rabbit polyclonal antibody (pAb ...
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bioRxiv - Immunology 2022Quote: ... The plate was washed five times after 1-hour secondary antibody incubation and developed with tetramethylbenzidine substrate (Biolegend, 421101). The reaction was stopped with 1 M phosphoric acid after 20 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained 20 min with APC-conjugated anti-CD24 antibody (1:100 dilution, 138506, Biolegend, San Diego, CA) and FITC-conjugated anti-Epcam antibody (1:500 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... Cochlea sections were also immunolabeled with rabbit anti-β3 tubulin (1:1000; clone TUJ1, Biolegend, San Diego, CA, USA) overnight at 4 °C followed by goat anti-rabbit Alexa-488 (1:200 ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were: Syn303 [a mouse monoclonal antibody that recognizes residues 1-5 of human α-synuclein (50)] (BioLegend) at 1:4,000 ...
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bioRxiv - Genomics 2022Quote: CRISPR/Cas9 RNP-treated Th2 cells were stained intracellularly with APC anti-GATA3 antibody (Biolegend #653806, 1:40 dilution) at room temperature for 30 minutes using True-Nuclear Transcription Factor Buffer Set (Biolegend #424401 ...
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bioRxiv - Cancer Biology 2022Quote: ... and lineage-negative cells (Lin-) were analyzed for c-Kit (clone 2B8; eBioscience) and Sca-1 (clone D7; BioLegend) expression to discriminate Kit+ Sca-1- (KL ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated 1:100 with antibodies directed against cell surface proteins CD11b-BV421 (BioLegend, 101251, clone M1/70), CD45-Percp-Cy5.5 (BioLegend ...
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bioRxiv - Cell Biology 2022Quote: ... Syt5/9 (SYSY, cat#105053), Tomosyn-1 (SYSY, cat#183103), Vamp2 (SYSY, Cat#104008) and Snap25 (Biolegend, Cat#836304) were used in Western blotting ...
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bioRxiv - Developmental Biology 2022Quote: ... the cell suspension was pelleted by centrifugation and cells were resuspended in FACS buffer containing 7AAD (BioLegend, 1:100). The flow cytometry analysis was performed on an LSR-II flow cytometer (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... Oxidative stress was induced using 0.03% H2O2 for 1 hour then cells were washed stained with viability dye Zombie Aqua (Biolegend) and placed on ice before immediate flow cytometry analysis.
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bioRxiv - Biochemistry 2020Quote: ... HA-Flag-tagged and GFP-tagged proteins were detected using HRP-conjugated monoclonal anti-HA (1:5000, BioLegend #901501) or polyclonal anti-GFP antibodies (1:2000 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and a further 1 h incubation with anti-His HRP conjugate (BioLegend; San Diego, CA, USA; cat. no. 652504) diluted (1:2500 ...
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bioRxiv - Immunology 2020Quote: ... The following antibodies were used for flow cytometry and stimulation: FITC-labelled anti-HLA-A2 (BB7.2, Biolegend, 1:40), AlexaFluor® 647-labeled anti-HLA-A,B,C (W6/32 ...
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bioRxiv - Microbiology 2020Quote: ... 1;100 dilution) and an antibody against SR-B1 for FACS (363208, clone m1B9; dilution 1:100) were from Biolegend. The SARS-2-S mAb (GTX632604 ...
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bioRxiv - Microbiology 2021Quote: ... in PBS containing the primary antibodies at a 1:100 dilution: mouse anti-DC/L-SIGN (Biolegend, cat. 845002), rabbit anti-DC-SIGN (Cell Signaling ...
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bioRxiv - Genomics 2021Quote: ... and then incubated 1:100 with antibodies directed against cell surface proteins CD11b-BV421 (BioLegend 101251, clone M1/70), CD45-APC (BioLegend 103112 ...
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bioRxiv - Immunology 2020Quote: ... and Tregs (anti-CD3-BV510, anti-CD4-PE, anti-CD8-AF700, anti-CD25-APC (1:100, Biolegend, Cat#302610), anti-FoxP3-AF488 (1:100 ...
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bioRxiv - Immunology 2020Quote: ... Th-17 (anti-CD3-BV510, anti-CD4-PE, anti-CD8-AF700, anti-IL17A-APC (1:100, Biolegend, Cat#512334)) and Tregs (anti-CD3-BV510 ...
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bioRxiv - Neuroscience 2021Quote: ... The cell pellet was then stained with antibodies to microglial cell surface markers (CD11b-PE Cy7, 1:200 Biolegend, # 101216 ...
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bioRxiv - Cancer Biology 2020Quote: ... and F480 (BM8, #123114), Gr-1 (clone RB6-8C5, #108428), Ly6G (Clone 1A8; #127645) and Ly6C (Clone HK1.4, #128030) from BioLegend. After 30 minutes of staining ...
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bioRxiv - Systems Biology 2021Quote: ... and then stained the cells for 30 min with 1:500 anti-NGFR APC-labeled clone ME20.4 (BioLegend, 345107). We washed the cells five times with 0.1% BSA/PBS and followed with one final wash with PBS for 2 min at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... Enriched EPCs from Day 10 were examined by staining with APC Anti-Human CD71 antibody (334107; Biolegend; 1:100) and incubating for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... and DAPI/conjugated PECAM1 antibody (0.04 µg/ml DAPI, Sigma, 10236276001; 1:250 Alexa647-anti-human PECAM1, Biolegend, 303112) in blocking buffer for 1 hr each ...
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bioRxiv - Immunology 2021Quote: ... Antibodies used to stain for IL-4Rα were APC anti-human CD124 (IL-4Rα) (Biolegend, clone G077F6, 1:100) and Live/Dead Fixable Green (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used for intracellular staining: Anti-human TBET FITC (1:50, clone 4B10, BioLegend, cat # 644812), Anti-human Granzyme B FITC (1:100 ...
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bioRxiv - Immunology 2020Quote: ... Cells were then stained for surface markers followed by intracellular cytokines using antibodies specific for IFN-y (4S.B3, 1:40) (Biolegend) IL-2 (MQ1-17H12 ...
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bioRxiv - Immunology 2020Quote: ... and incubated with biotin conjugated anti-CD8 (53-6.7) and anti-CD19 (6D5) at a dilution of 1:200 (both from Biolegend) and 50μL/mL normal rat serum for 10 min at RT ...