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• DOCK2-deficiency causes defects in anti-viral T cell responses and poor control of herpes simplex virus infection

  Katrina L. Randall, et al., bioRxiv - Immunology 2023

  Quote: ... PerCP Cy 5.5-conjugated anti-CD3 (BioLegend, 17A2), PE-conjugated anti-CD3 (BD ...
• DOCK2-deficiency causes defects in anti-viral T cell responses and poor control of herpes simplex virus infection

  Katrina L. Randall, et al., bioRxiv - Immunology 2023

  Quote: ... Brilliant Violet (BV) 605 conjugated anti-CD62L (Biolegend, MEL-14), PB-conjugated anti-CD44 (BioLegend ...
• DOCK2-deficiency causes defects in anti-viral T cell responses and poor control of herpes simplex virus infection

  Katrina L. Randall, et al., bioRxiv - Immunology 2023

  Quote: ... PB-conjugated anti-CD44 (BioLegend, IM7), APC-Cy7-conjugated live/dead stain ...
• DOCK2-deficiency causes defects in anti-viral T cell responses and poor control of herpes simplex virus infection

  Katrina L. Randall, et al., bioRxiv - Immunology 2023

  Quote: ... Biotin staining was followed by addition of BV605-conjugated streptavidin (BioLegend). For intracellular staining of FOXP3 ...
• Folate receptor overexpression shortens C. elegans lifespan by impeding adaptation to microbial metabolism

  Bideep Shrestha, Milla Tallila, Olli Matilainen, bioRxiv - Genetics 2023

  Quote: ... 1-16 Antibody (6E10) (used with 1:1000 dilution) was purchased from BioLegend. Anti-S6 Ribosomal Protein Antibody (used with 1:1000 dilution ...
• Decreased efficacy of a COVID-19 vaccine due to mutations present in early SARS-CoV-2 variants of concern

  Payton A.-B. Weidenbacher, et al., bioRxiv - Biochemistry 2023

  Quote: ... HRP goat anti-mouse (BioLegend 405306) was added at a 1:10,000 dilution in diluent buffer for 1 h at room temperature ...
• Metabolic regulation in erythroid differentiation by systemic ketogenesis in fasted mice

  Wenjuan Ma, et al., bioRxiv - Cell Biology 2023

  Quote: ... CD48 (HM48-1, APC-Cy7; BioLegend), Ter119 (TER-119 ...
• ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion

  Demis Menolfi, et al., bioRxiv - Genetics 2023

  Quote: ... and APC anti-mouse TER119 (Biolegend, 116212). Live cells were gated based on forward ...
• Gene editing without ex vivo culture evades genotoxicity in human hematopoietic stem cells

  Jing Zeng, et al., bioRxiv - Genetics 2023

  Quote: ... hCD33_PE (BioLegend, cat# 366608, RRID: AB_2566107), hCD3_PE_Cy7 (BioLegend ...
• ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion

  Demis Menolfi, et al., bioRxiv - Genetics 2023

  Quote: ... splenic B cells were collected and stained with FITC anti-mouse/human GL7 (Biolegend, Cat. No. 144604) and PE hamster anti-mouse CD95 (BD ...
• Gene editing without ex vivo culture evades genotoxicity in human hematopoietic stem cells

  Jing Zeng, et al., bioRxiv - Genetics 2023

  Quote: ... hCD34_FITC (BioLegend, cat# 343504, RRID: AB_1731852), were added to the cells and incubated on ice for 30 min ...
• Gene editing without ex vivo culture evades genotoxicity in human hematopoietic stem cells

  Jing Zeng, et al., bioRxiv - Genetics 2023

  Quote: ... hCD235a_FITC (BioLegend, cat# 349104, RRID: AB_10613463), hCD19_APC (BioLegend ...
• ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion

  Demis Menolfi, et al., bioRxiv - Genetics 2023

  Quote: ... with each antibody used at 1:200 dilution: FITC anti-mouse CD43 (Biolegend, 553270); PE anti-mouse IgM (Southern Biotech ...
• Evolution of chromosome arm aberrations in breast cancer through genetic network rewiring

  Elena Kuzmin, et al., bioRxiv - Genomics 2023

  Quote: ... and PE/Cy7 anti-mouse H2Kd antibody clone SF1-1.1 (Biolegend, # 116622). All cells were maintained at 37°C ...
• PP2A-B55^SUR-6 promotes nuclear envelope breakdown in C. elegans embryos

  Sukriti Kapoor, Kuheli Adhikary, Sachin Kotak, bioRxiv - Cell Biology 2023

  Quote: ... in combination with 1:200 rabbit anti-mAB414 (Biolegend: 902907). Embryos were fixed in methanol at –20°C for 30 minutes and incubated with primary antibodies for one hour at room temperature ...
• Metabolic regulation in erythroid differentiation by systemic ketogenesis in fasted mice

  Wenjuan Ma, et al., bioRxiv - Cell Biology 2023

  Quote: ... CD150 (TC15-12F12.2, BV-421; BioLegend), CD48 (HM48-1 ...
• Rationally designed multimeric nanovaccines using icosahedral DNA origami for molecularly controlled display of SARS-CoV-2 receptor binding domain

  Qingqing Feng, et al., bioRxiv - Bioengineering 2023

  Quote: ... anti-mouse CD4 PerCP (Biolegend, Cat: #100432).
• Rationally designed multimeric nanovaccines using icosahedral DNA origami for molecularly controlled display of SARS-CoV-2 receptor binding domain

  Qingqing Feng, et al., bioRxiv - Bioengineering 2023

  Quote: ... anti-mouse CXCR5 APC (Biolegend, Cat: #145506), anti-mouse CD103 APC (Biolegend ...
• Rationally designed multimeric nanovaccines using icosahedral DNA origami for molecularly controlled display of SARS-CoV-2 receptor binding domain

  Qingqing Feng, et al., bioRxiv - Bioengineering 2023

  Quote: ... anti-mouse CD138 APC (Biolegend, Cat: #142506), anti-mouse CXCR5 APC (Biolegend ...
• Rationally designed multimeric nanovaccines using icosahedral DNA origami for molecularly controlled display of SARS-CoV-2 receptor binding domain

  Qingqing Feng, et al., bioRxiv - Bioengineering 2023

  Quote: ... anti-mouse IFNγ PE/Cyanine7 (Biolegend, Cat: #505826), anti-mouse F4/80 PE/Cyanine7 (Biolegend ...
• Rationally designed multimeric nanovaccines using icosahedral DNA origami for molecularly controlled display of SARS-CoV-2 receptor binding domain

  Qingqing Feng, et al., bioRxiv - Bioengineering 2023

  Quote: ... anti-mouse CD103 APC (Biolegend, Cat: #121414), anti-mouse CD8 FITC (Biolegend ...
• Rationally designed multimeric nanovaccines using icosahedral DNA origami for molecularly controlled display of SARS-CoV-2 receptor binding domain

  Qingqing Feng, et al., bioRxiv - Bioengineering 2023

  Quote: ... anti-mouse CD103 PE (Biolegend, Cat: #121406), anti-mouse CD21/CD35 PE (Biolegend ...
• Rationally designed multimeric nanovaccines using icosahedral DNA origami for molecularly controlled display of SARS-CoV-2 receptor binding domain

  Qingqing Feng, et al., bioRxiv - Bioengineering 2023

  Quote: ... anti-mouse CXCR3 PE (Biolegend, Cat: #126505), anti-mouse CD103 PE (Biolegend ...
• Rationally designed multimeric nanovaccines using icosahedral DNA origami for molecularly controlled display of SARS-CoV-2 receptor binding domain

  Qingqing Feng, et al., bioRxiv - Bioengineering 2023

  Quote: ... anti-mouse CD11C FITC (Biolegend, Cat: #117306), anti-mouse CD95 (Fas ...
• Versatile Tissue-Injectable Hydrogels with Extended Hydrolytic Release of Bioactive Protein Therapeutics

  Eric S. Nealy, et al., bioRxiv - Bioengineering 2023

  Quote: All antibodies and viability dyes were purchased from Biolegend (San Diego, CA, USA). Zombie AquaTM live dead ...
• Rationally designed multimeric nanovaccines using icosahedral DNA origami for molecularly controlled display of SARS-CoV-2 receptor binding domain

  Qingqing Feng, et al., bioRxiv - Bioengineering 2023

  Quote: ... anti-mouse/human GL7 PE/Cyanine7 (Biolegend, Cat: #144619), anti-mouse EpCAM PE/Cyanine7 (Biolegend ...
• Rationally designed multimeric nanovaccines using icosahedral DNA origami for molecularly controlled display of SARS-CoV-2 receptor binding domain

  Qingqing Feng, et al., bioRxiv - Bioengineering 2023

  Quote: ... anti-mouse CD8 FITC (Biolegend, Cat: #100706), anti-mouse CD11C FITC (Biolegend ...
• Rationally designed multimeric nanovaccines using icosahedral DNA origami for molecularly controlled display of SARS-CoV-2 receptor binding domain

  Qingqing Feng, et al., bioRxiv - Bioengineering 2023

  Quote: ... anti-mouse CD3 APC (BioLegend, Cat: #100312), anti-mouse CD19 APC (BioLegend ...
• Rationally designed multimeric nanovaccines using icosahedral DNA origami for molecularly controlled display of SARS-CoV-2 receptor binding domain

  Qingqing Feng, et al., bioRxiv - Bioengineering 2023

  Quote: ... anti-mouse CD21/CD35 PE (Biolegend, Cat: #123410), anti-mouse CD69 PE (Biolegend ...
• Rationally designed multimeric nanovaccines using icosahedral DNA origami for molecularly controlled display of SARS-CoV-2 receptor binding domain

  Qingqing Feng, et al., bioRxiv - Bioengineering 2023

  Quote: ... anti-mouse F4/80 PE/Cyanine7 (Biolegend, Cat: #123114), anti-mouse CD279 (PD-1 ...
• Rationally designed multimeric nanovaccines using icosahedral DNA origami for molecularly controlled display of SARS-CoV-2 receptor binding domain

  Qingqing Feng, et al., bioRxiv - Bioengineering 2023

  Quote: ... anti-mouse CD69 PE (Biolegend, Cat: #104508), anti-mouse CD44 PE (Biolegend ...
• Rationally designed multimeric nanovaccines using icosahedral DNA origami for molecularly controlled display of SARS-CoV-2 receptor binding domain

  Qingqing Feng, et al., bioRxiv - Bioengineering 2023

  Quote: ... anti-mouse CD19 APC (BioLegend, Cat: #115512), anti-mouse CD138 APC (Biolegend ...
• Selective Inhibition of Interleukin-2 Inducible T Cell Kinase (ITK) Enhances Anti-Tumor Immunity in Association with Th1-skewing, Cytotoxic T cell Activation, and Reduced T Cell Exhaustion

  Lih-Yun Hsu, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Live naïve CD4+ T cells were purified from PBMCs post staining with fluorescence labelled antibodies to human CD3(OKT3, Biolegend), CD4 (RPA-T4 ...
• Selective Inhibition of Interleukin-2 Inducible T Cell Kinase (ITK) Enhances Anti-Tumor Immunity in Association with Th1-skewing, Cytotoxic T cell Activation, and Reduced T Cell Exhaustion

  Lih-Yun Hsu, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-human IL-4 (OA19A66, Biolegend) and anti-human IL-5 (TRFK5 ...
• Selective Inhibition of Interleukin-2 Inducible T Cell Kinase (ITK) Enhances Anti-Tumor Immunity in Association with Th1-skewing, Cytotoxic T cell Activation, and Reduced T Cell Exhaustion

  Lih-Yun Hsu, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Biolegend)neutralizing antibodies (10 ug/ml each, Biolegend); Th2 condition ...
• Selective Inhibition of Interleukin-2 Inducible T Cell Kinase (ITK) Enhances Anti-Tumor Immunity in Association with Th1-skewing, Cytotoxic T cell Activation, and Reduced T Cell Exhaustion

  Lih-Yun Hsu, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Permeabilized cells were stained with antibodies against IFNγ (XMG1.2, Biolegend), and TNF (MP6-XT22 ...
• Selective Inhibition of Interleukin-2 Inducible T Cell Kinase (ITK) Enhances Anti-Tumor Immunity in Association with Th1-skewing, Cytotoxic T cell Activation, and Reduced T Cell Exhaustion

  Lih-Yun Hsu, et al., bioRxiv - Cancer Biology 2023

  Quote: Cells were stained with the following antibodies against mouse CD45 (30-F1, Biolegend), CD8 (53-6.7 ...
• Transcription Factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress

  Milad Soleimani, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Primary antibodies against CD44 (BioLegend, 338806), CD24 (BioLegend ...
• Transcription Factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress

  Milad Soleimani, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and CD49f (BioLegend, 313612) were diluted in the flow wash buffer and incubated with cells for 30min at room temperature ...
• Transcription Factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress

  Milad Soleimani, et al., bioRxiv - Cancer Biology 2023

  Quote: ... CD24 (BioLegend, 311104), and CD49f (BioLegend ...
• Multiple myeloma long-term survivors display sustained immune alterations decades after first line therapy

  Raphael Lutz, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Drop-in CD27 Brilliant Violet 510 (clone O323, Biolegend, San Diego, USA) and CD138 Brilliant Violet 421 (clone MI15 ...
• Caspase-2 is essential for proliferation and self-renewal of nucleophosmin-mutated acute myeloid leukemia

  Dharaniya Sakthivel, et al., bioRxiv - Cancer Biology 2023

  Quote: ... APC anti-human CD14 (clone 63D3; BioLegend). Alexa Fluor 647 Phalloidin was purchased from Cell Signaling Technology ...
• A small molecule inhibitor of PTP1B and PTPN2 enhances T cell anti-tumor immunity

  Shuwei Liang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Ly49H (clone 3D10, BioLegend Cat# 144702) antibody for NK stimulation was from BioLegend ...
• A small molecule inhibitor of PTP1B and PTPN2 enhances T cell anti-tumor immunity

  Shuwei Liang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... PB or Brilliant Ultra Violet (BUV) 395-conjugated CD8 (BioLegend Cat# 100759 ...
• Human colorectal cancer: upregulation of the adaptor protein Rai in TILs leads to cell dysfunction by sustaining GSK-3 activation and PD-1 expression

  Tommaso Montecchi, et al., bioRxiv - Cancer Biology 2023

  Quote: ... At the end of the incubation cells were stained with PE anti-CD8 antibody (clone RPA-T8, Biolegend #301008) and the degranulation extent was measured among the GFP+ population as the percentage of CD8+ T cells expressing LAMP1 on their surface by flow cytometry analysis.
• Human colorectal cancer: upregulation of the adaptor protein Rai in TILs leads to cell dysfunction by sustaining GSK-3 activation and PD-1 expression

  Tommaso Montecchi, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Transfected cells were then stimulated for 24h at 37°C with plate bound 1μg/ml of anti-CD3 (clone UCHT1, BioLegend #300402) and 2μg/ml of anti-CD28 (clone CD28.2 ...
• Human colorectal cancer: upregulation of the adaptor protein Rai in TILs leads to cell dysfunction by sustaining GSK-3 activation and PD-1 expression

  Tommaso Montecchi, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Jurkat transfectants were activated for 5 minutes at 37°C in serum-free RPMI-1640-sodium bicarbonate by crosslinking the anti-CD3 antibody (1μg/ml, clone UCHT1, BioLegend #300402) with secondary anti-mouse antibodies for immunoblot analysis of GSK-3 α/β and Akt phosphorylation.
• Human colorectal cancer: upregulation of the adaptor protein Rai in TILs leads to cell dysfunction by sustaining GSK-3 activation and PD-1 expression

  Tommaso Montecchi, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Monensin (2mM, Biolegend #420701) was added 1h after the incubation start to block vesicular recycling ...
• Phosphoprotein dynamics of interacting tumor and T cells by HySic

  Sofía Ibáñez-Molero, et al., bioRxiv - Cancer Biology 2023

  Quote: ... totalPAK1 (1:500, Biolegend, 868601), peroxidase conjugated anti-mouse IgG (1:5000 ...
• Anti-CD117 CAR T cells incorporating a safety switch eradicate human acute myeloid leukemia and hematopoietic stem cells

  Chiara F. Magnani, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and IL-2 were investigated with an enzyme-linked immunosorbent assay (ELISA MAXTM Deluxe Set, Biolegend®, San Diego, CA, USA) according to the manufacturer’s instruction ...