Labshake search

7251 - 7300 of 10000+ citations since 2019

• Prolonged T cell – DC macro-clustering within lymph node microenvironments initiates Th2 cell differentiation in a site-specific manner

  Miranda R. Lyons-Cohen, et al., bioRxiv - Immunology 2023

  Quote: ... IRF4 (clone IRF4.3E4; BioLegend), CD45.1 (clone A20 ...
• Prolonged T cell – DC macro-clustering within lymph node microenvironments initiates Th2 cell differentiation in a site-specific manner

  Miranda R. Lyons-Cohen, et al., bioRxiv - Immunology 2023

  Quote: ... MHCII (clone M5/114.15.2; BioLegend), IRF4 (clone IRF4.3E4 ...
• Prolonged T cell – DC macro-clustering within lymph node microenvironments initiates Th2 cell differentiation in a site-specific manner

  Miranda R. Lyons-Cohen, et al., bioRxiv - Immunology 2023

  Quote: ... CD11b (clone M1/70; Biolegend), MHCII (clone M5/114.15.2 ...
• Prolonged T cell – DC macro-clustering within lymph node microenvironments initiates Th2 cell differentiation in a site-specific manner

  Miranda R. Lyons-Cohen, et al., bioRxiv - Immunology 2023

  Quote: ... (XMG1.2, Biolegend), or Th2 (anti-IFNγ 10µg/mL (XMG1.2 ...
• Prolonged T cell – DC macro-clustering within lymph node microenvironments initiates Th2 cell differentiation in a site-specific manner

  Miranda R. Lyons-Cohen, et al., bioRxiv - Immunology 2023

  Quote: ... CD19 (clone 6D5; BioLegend), CD44 (clone IM7 ...
• Targeting human γδ T cells as a potent and safe alternative to pan-T cells bispecific cell engagers

  Lola Boutin, et al., bioRxiv - Immunology 2023

  Quote: ... PE-conjugated anti-PD1 and FITC-conjugated anti-Vγ9 mAbs were purchased from BioLegend. BV510-conjugated anti-CD4 and BV421-conjugated anti-CD8 mAbs were purchased from BD Bioscience ...
• Mmp17-deficient mice exhibit heightened goblet cell effector expression in the colon and increased resistance to chronic Trichuris muris infection

  Pia M. Vornewald, et al., bioRxiv - Immunology 2023

  Quote: ... 96 well plates were coated with purified anti-mouse IgE (2ug/ml, Biolegend, Clone: RME-1) in 0.05M carbonate/ bicarbonate buffer and incubated overnight at 47C ...
• optiPRM: A targeted immunopeptidomics LC-MS workflow with ultra-high sensitivity for the detection of mutation-derived tumor neoepitopes from limited input material

  Mogjiborahman Salek, et al., bioRxiv - Immunology 2023

  Quote: ... SA-allophycocyanin (SA-APC, BioLegend), SA-Brilliant Violet 421 (SA-BV421 ...
• Sensitization with an allogeneic MHC class I molecule induces anti-tumor immunity in the absence of PD-1 in mice

  Komang Alit Paramitasari, Yasumasa Ishida, bioRxiv - Immunology 2023

  Quote: ... Immunostaining was performed using the following primary antibodies: rat anti-mouse CD8α (clone 53-6.7, eBioscience) and Armenian hamster anti-mouse CD11c (clone N418, BioLegend). CD8 primary staining signal was further amplified using an anti-rat IgG secondary antibody conjugated with Alexa Fluor (AF ...
• Altered DNA methylation underlies monocyte dysregulation and innate exhaustion memory in sepsis

  Blake A. Caldwell, Yajun Wu, Jing Wang, Liwu Li, bioRxiv - Immunology 2023

  Quote: ... and CD11b (APC-Cy7; Biolegend #101226). Cells were then washed with FACS buffer (HBSS with 2% fetal bovine serum ...
• Altered DNA methylation underlies monocyte dysregulation and innate exhaustion memory in sepsis

  Blake A. Caldwell, Yajun Wu, Jing Wang, Liwu Li, bioRxiv - Immunology 2023

  Quote: ... CD68 (APC-Fire750; Biolegend #137041), CD172a (BV510 ...
• Altered DNA methylation underlies monocyte dysregulation and innate exhaustion memory in sepsis

  Blake A. Caldwell, Yajun Wu, Jing Wang, Liwu Li, bioRxiv - Immunology 2023

  Quote: ... cells were stained with antibodies against Ly6C (Pacific Blue; Biolegend #128014), CD11b (BV650 ...
• Altered DNA methylation underlies monocyte dysregulation and innate exhaustion memory in sepsis

  Blake A. Caldwell, Yajun Wu, Jing Wang, Liwu Li, bioRxiv - Immunology 2023

  Quote: ... and CD38 (PE; Biolegend #102708). For Panel 2 ...
• Unanticipated interacting features of human gut-associated lymphoid tissues link microbiota, intestinal immunity and autoimmunity

  Lucia Montorsi, et al., bioRxiv - Immunology 2023

  Quote: ... CD20 (Biolegend, #382802). Slides were washed twice in PBS-T for 5min and incubated for 1h with anti-rabbit AlexaFluor555 (Invitrogen ...
• Regulation of BCR-mediated Ca²⁺ mobilization by MIZ1-TIMBIM4 safeguards IgG1⁺ GC B cell positive selection

  Lingling Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... BV421 anti-mouse IgD Antibody (Clone 11-26c.2a; BioLegend; Cat#405725; RRID: AB_2562743) BUV395 Rat Anti-Mouse IgD (Clone 11-26c.2a ...
• Regulation of BCR-mediated Ca²⁺ mobilization by MIZ1-TIMBIM4 safeguards IgG1⁺ GC B cell positive selection

  Lingling Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... BV605 Rat Anti-Mouse CD86 (Clone GL1; BioLegend; Cat#105037; RRID: AB_11204429) BV510 Armenian Hamster Anti-Mouse CD95 (Clone Jo2 ...
• Regulation of BCR-mediated Ca²⁺ mobilization by MIZ1-TIMBIM4 safeguards IgG1⁺ GC B cell positive selection

  Lingling Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... Alexa Fluor® 700 anti-mouse IgD Antibody (Clone 11-26c.2a; BioLegend; Cat#405730; RRID: AB_2563341) Alexa Fluor® 488 anti-mouse IgD Antibody (Clone 11-26c.2a ...
• Regulation of BCR-mediated Ca²⁺ mobilization by MIZ1-TIMBIM4 safeguards IgG1⁺ GC B cell positive selection

  Lingling Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... BV605 Mouse Anti-Mouse CD45.1 (Clone A20; BioLegend; Cat#110738; RRID: AB_2562565) BUV395 Mouse Anti-Mouse CD45.2 (Clone 104 ...
• Regulation of BCR-mediated Ca²⁺ mobilization by MIZ1-TIMBIM4 safeguards IgG1⁺ GC B cell positive selection

  Lingling Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... Alexa Fluor® 488 anti-mouse IgD Antibody (Clone 11-26c.2a; BioLegend; Cat#405718; RRID: AB_10730619) BV421 anti-mouse IgD Antibody (Clone 11-26c.2a ...
• Regulation of BCR-mediated Ca²⁺ mobilization by MIZ1-TIMBIM4 safeguards IgG1⁺ GC B cell positive selection

  Lingling Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... RRID: AB_10670489) PerCP/Cyanine5.5 anti-mouse IgD Antibody (Clone 11-26c.2a; BioLegend; Cat#405710; RRID: AB_1575113) Alexa Fluor® 700 anti-mouse IgD Antibody (Clone 11-26c.2a ...
• Regulation of BCR-mediated Ca²⁺ mobilization by MIZ1-TIMBIM4 safeguards IgG1⁺ GC B cell positive selection

  Lingling Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... TER-119 (clone TER-119, Biolegend) negative selection using anti-mouse biotinylated antibodies and anti-Biotin Microbeads (Miltenyi Biotec) ...
• Breakthrough infection elicits hypermutated IGHV3-53/3-66 public antibodies with broad and potent neutralizing activity against SARS-CoV-2 variants including BQ and XBB lineages

  Ling Li, et al., bioRxiv - Immunology 2023

  Quote: ... CD19-PE/Cy7 (BioLegend, 302215), CD27-APC (BioLegend ...
• CARs are organized in nanodomains in the plasma membrane of T cells that accumulate at tumor contact sites

  Christina Verbruggen, et al., bioRxiv - Immunology 2023

  Quote: ... CD3 (Hit3a) and matched isotype controls (all BioLegend, San Diego, CA, USA). CAR-transduced (thus EGFRt+ ...
• CARs are organized in nanodomains in the plasma membrane of T cells that accumulate at tumor contact sites

  Christina Verbruggen, et al., bioRxiv - Immunology 2023

  Quote: ... the cells were stained as described for dSTORM analysis with antibodies against scFv-Linker (evitria) or CD20 antibody (clone 2H7, Biolegend). Samples were stored at 4°C until imaging.
• CARs are organized in nanodomains in the plasma membrane of T cells that accumulate at tumor contact sites

  Christina Verbruggen, et al., bioRxiv - Immunology 2023

  Quote: ... CAR-transduced (thus EGFRt+) T cells were detected by staining with anti-EGFR antibody (clone AY13, Biolegend, San Diego, CA, USA) and scFv-Linker (custom made ...
• Breakthrough infection elicits hypermutated IGHV3-53/3-66 public antibodies with broad and potent neutralizing activity against SARS-CoV-2 variants including BQ and XBB lineages

  Ling Li, et al., bioRxiv - Immunology 2023

  Quote: ... CD38-APC/Cy7 (BioLegend, 356615), human IgM-AF700 (BioLegend ...
• Breakthrough infection elicits hypermutated IGHV3-53/3-66 public antibodies with broad and potent neutralizing activity against SARS-CoV-2 variants including BQ and XBB lineages

  Ling Li, et al., bioRxiv - Immunology 2023

  Quote: ... CD27-APC (BioLegend, 356409), CD38-APC/Cy7 (BioLegend ...
• Breakthrough infection elicits hypermutated IGHV3-53/3-66 public antibodies with broad and potent neutralizing activity against SARS-CoV-2 variants including BQ and XBB lineages

  Ling Li, et al., bioRxiv - Immunology 2023

  Quote: ... human IgD-perCP/Cy5.5 (BioLegend, 348207), anti-His-FITC (Proteintech ...
• Breakthrough infection elicits hypermutated IGHV3-53/3-66 public antibodies with broad and potent neutralizing activity against SARS-CoV-2 variants including BQ and XBB lineages

  Ling Li, et al., bioRxiv - Immunology 2023

  Quote: ... anti-FLAG-PE (BioLegend, 637309) and DAPI ...
• Breakthrough infection elicits hypermutated IGHV3-53/3-66 public antibodies with broad and potent neutralizing activity against SARS-CoV-2 variants including BQ and XBB lineages

  Ling Li, et al., bioRxiv - Immunology 2023

  Quote: ... human IgM-AF700 (BioLegend, 314537), human IgD-perCP/Cy5.5 (BioLegend ...
• Dysregulation of γδ intraepithelial lymphocytes precedes Crohn’s disease-like ileitis

  Weili Xu, et al., bioRxiv - Immunology 2023

  Quote: ... anti-CD44 (1M7, Biolegend), anti-CD5 (53.7-3 ...
• HIF-1α-mediated mitochondrial-glycolytic reprogramming controls the transition of precursor to terminally exhausted T cells

  Hao Wu, et al., bioRxiv - Immunology 2023

  Quote: ... were isolated from single cell suspension of lymph nodes and spleen by negative selection using the MojoSort Mouse CD8 T cell isolation kit (BioLegend). Alternatively ...
• HIF-1α-mediated mitochondrial-glycolytic reprogramming controls the transition of precursor to terminally exhausted T cells

  Hao Wu, et al., bioRxiv - Immunology 2023

  Quote: ... CD8+ T cells were labelled with anti-CD8α-APC (Biolegend, clone 53-6.7) and positively enriched using anti-APC microbeads (Miltenyi Biotech) ...
• Rewiring of the TCR signalosome in natural intestinal Intraepithelial T lymphocytes drives non-deletional tolerance

  Harriet J. Watt, et al., bioRxiv - Immunology 2023

  Quote: ... followed by anti-mouse PE (Biolegend), LAT (1:200) ...
• Rewiring of the TCR signalosome in natural intestinal Intraepithelial T lymphocytes drives non-deletional tolerance

  Harriet J. Watt, et al., bioRxiv - Immunology 2023

  Quote: ... Stained T-IEL and unstained lymph node T cells were mixed at a 1:1 ratio before stimulation with 30 μg/ml anti-CD3 (Clone 145-2C11, Biolegend) crosslinked with 5 μg/ml anti-Hamster (Jackson Immunoresearch) ...
• Rewiring of the TCR signalosome in natural intestinal Intraepithelial T lymphocytes drives non-deletional tolerance

  Harriet J. Watt, et al., bioRxiv - Immunology 2023

  Quote: Mice were treated with 50 µg anti-CD3 Ultra-LEAF (Clone 145-2C11) (Biolegend) or sterile PBS (Gibco ...
• Rewiring of the TCR signalosome in natural intestinal Intraepithelial T lymphocytes drives non-deletional tolerance

  Harriet J. Watt, et al., bioRxiv - Immunology 2023

  Quote: ... followed by anti-rabbit Dylight649 (1:500) (Biolegend), NTAL-PE (1:100 ...
• Rewiring of the TCR signalosome in natural intestinal Intraepithelial T lymphocytes drives non-deletional tolerance

  Harriet J. Watt, et al., bioRxiv - Immunology 2023

  Quote: ... and cKit-APC-Cy7 (Biolegend). Lineage-negative ...
• Rewiring of the TCR signalosome in natural intestinal Intraepithelial T lymphocytes drives non-deletional tolerance

  Harriet J. Watt, et al., bioRxiv - Immunology 2023

  Quote: ... CD4 and CD8β cells were depleted from the T-IEL compartment using CD4 and CD8β biotinylated antibodies and magnetic streptavidin nanobeads (MojoSort) (Biolegend). CD8α+ cells were purified from CD4 and CD8β depleted T-IEL and lymph node T cells using the CD8α Positive Selection Kit (Stemcell Technologies) ...
• Upregulated Fcrl5 disrupts B cell anergy and causes autoimmunity

  Chisato Ono, et al., bioRxiv - Immunology 2023

  Quote: ... TER-119 (TER-119) (all from BioLegend, BD Biosciences ...
• Upregulated Fcrl5 disrupts B cell anergy and causes autoimmunity

  Chisato Ono, et al., bioRxiv - Immunology 2023

  Quote: ... or MojoSort Mouse Pan B cell Isolation Kit (BioLegend), following the manufacturer’s instructions ...
• Dendritic Cells Overcome Cre/Lox Induced Gene Deficiency by Siphoning Material From Neighboring Cells Using Intracellular Monitoring—a Novel Mechanism of Antigen Acquisition

  Christopher H. Herbst, et al., bioRxiv - Immunology 2023

  Quote: ... Blocking antibodies specific for the following proteins were used: 2.5 μg/ml CD11b (M1/70, BioLegend, #101224), 2.5 μg/ml CD11c (N418 ...
• Dendritic Cells Overcome Cre/Lox Induced Gene Deficiency by Siphoning Material From Neighboring Cells Using Intracellular Monitoring—a Novel Mechanism of Antigen Acquisition

  Christopher H. Herbst, et al., bioRxiv - Immunology 2023

  Quote: ... A single cells suspension was generated and stained with eBioscience’s Fixable Viability Dye eFluor780 and anti-CD90.2 (30-H12, BioLegend, #105308) and anti-CD19 (6D5 ...
• IL-15 enhances anti-tumor immunity of sipuleucel-T by activating lymphocyte subsets and reversing immunoresistance

  Muhammad A. Saeed, et al., bioRxiv - Immunology 2023

  Quote: ... ELISA for IFN-γ and IL-10 were also performed with human ELISA MAX Deluxe Sets (BioLegend) to verify the multiplex ELISA results ...
• Inducible nitric oxide synthase (iNOS) is necessary for GBP-mediated T. gondii restriction in murine macrophages via vacuole nitration and intravacuolar network collapse

  Xiao-Yu Zhao, et al., bioRxiv - Immunology 2023

  Quote: ... surface markers were stained with following antibodies at 1:200 dilution at 4°C for 30 min: CD11b- Pacific Blue (BioLegend, 101224), CD45-BV650 (BioLegend ...
• Inducible nitric oxide synthase (iNOS) is necessary for GBP-mediated T. gondii restriction in murine macrophages via vacuole nitration and intravacuolar network collapse

  Xiao-Yu Zhao, et al., bioRxiv - Immunology 2023

  Quote: ... CD45-BV650 (BioLegend, 103151), CD11c-BV711 (BioLegend ...
• Inducible nitric oxide synthase (iNOS) is necessary for GBP-mediated T. gondii restriction in murine macrophages via vacuole nitration and intravacuolar network collapse

  Xiao-Yu Zhao, et al., bioRxiv - Immunology 2023

  Quote: ... I-A/I-E-AF647 ((BioLegend, 107618), Ly6G-APC/Cy7 (BioLegend ...
• Five inhibitory receptors display distinct vesicular distributions in T cells

  Jiahe Lu, et al., bioRxiv - Immunology 2023

  Quote: ... CL4 offspring were phenotyped by staining PBMCs with anti-CD8-APC (53-6.7, Biolegend, RRID: AB_312750) and anti-Vβ8.2 FITC (KJ16-133 ...
• Soluble CTLA-4 mainly produced by Treg cells inhibits type 1 inflammation without hindering type 2 immunity to allow for inflammation resolution

  Motonao Osaki, Shimon Sakaguchi, bioRxiv - Immunology 2023

  Quote: ... anti-mCD200R3 (Ba13, BioLegend, 142206), anti-mCD24 (M1/69 ...
• Soluble CTLA-4 mainly produced by Treg cells inhibits type 1 inflammation without hindering type 2 immunity to allow for inflammation resolution

  Motonao Osaki, Shimon Sakaguchi, bioRxiv - Immunology 2023

  Quote: ... anti-mFcεRIα (MAR-1, BioLegend, 134304), anti-mCD49b (HMα2 ...