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• Spatiotemporal development of the human T follicular helper cell response to Influenza vaccination

  Stefan A Schattgen, et al., bioRxiv - Immunology 2023

  Quote: ... Ghost viability dye (Tonbo Biosciences),TCR a/b-BV421 (Clone: IP26, Biolegend), CD69-PerCP-eFluor-710(Clone ...
• Local CpG-Stat3 siRNA treatment improves antitumor effects of immune checkpoint inhibitors

  Chunyan Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... Cells were then incubated with FITC, and PE-conjugated antibodies (1:100, IFNr or FoxP3, GZMB) purchased from Biolegend. Aqua LIVE/DEAD used for cell viability was purchased from Invitrogen ...
• AI-based antibody discovery platform identifies novel, diverse and pharmacologically active therapeutic antibodies against multiple SARS-CoV-2 strains

  Cristina Moldovan Loomis, et al., bioRxiv - Immunology 2023

  Quote: ... MaxiSorpTM ELISA plates (Biolegend cat# 50-712-278) were coated with human ACE-2 Fc chimera protein (R&D Systems cat# 10544-ZN ...
• An Alzheimer’s disease risk variant in TTC3 modifies the actin cytoskeleton organization and the PI3K-Akt signaling pathway in iPSC-derived forebrain neurons

  Holly N. Cukier, et al., bioRxiv - Genetics 2023

  Quote: ... Purified anti-Tubulin β-3 (TUBB3) Antibody 1:1000 (Biolegend, #PRB-435P), Anti-MAP2 antibody 1:1000 (Abcam ...
• Exomap1 mouse: a transgenic model for in vivo studies of exosome biology

  Francis K. Fordjour, et al., bioRxiv - Cell Biology 2023

  Quote: ... mouse CD63 (Biolegend, Cat. No. 143902, San Diego, CA, USA), mouse CD81 (Biolegend ...
• Exomap1 mouse: a transgenic model for in vivo studies of exosome biology

  Francis K. Fordjour, et al., bioRxiv - Cell Biology 2023

  Quote: ... and human CD81(Biolegend, Cat. No. 349502, San Diego, CA, USA) (exomap1::Camk2a-Cre mouse and control exomap1+/ mouse) ...
• Exomap1 mouse: a transgenic model for in vivo studies of exosome biology

  Francis K. Fordjour, et al., bioRxiv - Cell Biology 2023

  Quote: ... These coverslips were then washed with PBS containing 0.025% Tween 20 and EVs were labeled with one of the following affinity reagents: 1) a mixture of AF647-labeled antibodies specific for mouse CD9 (Biolegend, Cat. No. 124802, San Diego, CA, USA), mouse CD63 (Biolegend ...
• Exomap1 mouse: a transgenic model for in vivo studies of exosome biology

  Francis K. Fordjour, et al., bioRxiv - Cell Biology 2023

  Quote: ... and human IgG were acquired from commercial sources (Biolegend).
• Emergence and suppressive function of Tr1 cells in glomerulonephritis

  Shiwa Soukou-Wargalla, et al., bioRxiv - Immunology 2023

  Quote: ... CD45.1 (APC, BioLegend, clone A20 ...
• Emergence and suppressive function of Tr1 cells in glomerulonephritis

  Shiwa Soukou-Wargalla, et al., bioRxiv - Immunology 2023

  Quote: ... CD279 PD1 (BV 605, BioLegend, clone 29F.1A12 ...
• Emergence and suppressive function of Tr1 cells in glomerulonephritis

  Shiwa Soukou-Wargalla, et al., bioRxiv - Immunology 2023

  Quote: ... CD366 (TIM-3) (BV 421, BioLegend, clone RMT3-23 ...
• Emergence and suppressive function of Tr1 cells in glomerulonephritis

  Shiwa Soukou-Wargalla, et al., bioRxiv - Immunology 2023

  Quote: ... CD45 (BV785, BioLegend, clone 30-F11 ...
• Maternal diet and gut microbiome composition modulate early life immune development

  Erica T Grant, et al., bioRxiv - Microbiology 2023

  Quote: ... Cells were stained with anti-CD4 BV605 (#100548, clone RM4-5, 1:700, Biolegend), anti-CD3 BV711 (#100241 ...
• Maternal diet and gut microbiome composition modulate early life immune development

  Erica T Grant, et al., bioRxiv - Microbiology 2023

  Quote: ... cells were incubated in 100 µl PBS 1X containing 1.5 μL Zombie NIR Fixable Viability kit (BioLegend, San Diego, CA, USA) at 4°C for 30 min ...
• ORF3c is expressed in SARS-CoV-2 infected cells and suppresses immune activation by inhibiting innate sensing

  Martin Müller, et al., bioRxiv - Microbiology 2023

  Quote: ... Rat polyclonal (IgG2a, κ) anti-human GAPDH (l:l000, BioLegend, Cat# 607902), Rabbit polyclonal anti-Sendai Virus (l:2000 ...
• The Hypoxia-regulated Ectonucleotidase CD73 is a Host Determinant of HIV Latency

  Hannah S. Sperber, et al., bioRxiv - Microbiology 2023

  Quote: ... cells were stained with APC anti-human CD73 (BioLegend, Cat. #344006) diluted in PBS (1:20 ...
• The Hypoxia-regulated Ectonucleotidase CD73 is a Host Determinant of HIV Latency

  Hannah S. Sperber, et al., bioRxiv - Microbiology 2023

  Quote: ... using the fixable Zombie viability dye (1:100; BioLegend, Cat. #423113) according to the manufacturer’s protocol ...
• Deep learning and CRISPR-Cas13d ortholog discovery for optimized RNA targeting

  Jingyi Wei, et al., bioRxiv - Bioengineering 2023

  Quote: ... and then blocked with Human TruStain FcX (Biolegend) for 10 minutes ...
• Selective inhibition of fibroblast-specific Domain Discoidin Receptor 1 (DDR1) reduces collagen deposition and modulates fibroblast-specific cytokine release within the breast microenvironment

  Daniel E.C. Fein, et al., bioRxiv - Cell Biology 2023

  Quote: ... CD45.2-PE (BioLegend 109807; 1:400), TCRβ-PE-Cy7 (BioLegend 109221 ...
• Centrosome amplification primes for apoptosis and favors the response to chemotherapy in ovarian cancer beyond multipolar divisions

  Frances Edwards, et al., bioRxiv - Cell Biology 2023

  Quote: ... Cells were stained using Annexin V APC and Propidium Iodide (Biolegend # 640932) at 0,6μg/mL and 10mg/mL respectively ...
• Multi-organ single-cell RNA-sequencing reveals early hyperglycaemia responses that converge on fibroblast dysregulation

  Adam T. Braithwaite, et al., bioRxiv - Cell Biology 2023

  Quote: ... cells were resuspended in 500 µl RPMI containing 10% FBS and labelled with commercially available DNA-labelled antibodies (TotalSeq-A, Biolegend) at 4 °C for 30 min ...
• Modular and integrative activity reporters enhance biochemical studies in the yeast ER

  Samantha G Martinusen, et al., bioRxiv - Synthetic Biology 2023

  Quote: ... OD600 of induced cultures was taken and used to determine approximate volumes of culture to stain two million cells with fluorescently labeled antibodies (anti-FLAG PE, Biolegend, cat# 637309, anti-HA Alexa 647, Biolegend, cat #682404). Cells were washed in PBS 0.5% BSA (0.5% BSA ...
• Modular and integrative activity reporters enhance biochemical studies in the yeast ER

  Samantha G Martinusen, et al., bioRxiv - Synthetic Biology 2023

  Quote: ... OD600 of induced cultures was taken and used to determine approximate volumes of culture to stain two million cells with fluorescently labeled antibodies (anti-FLAG PE, Biolegend, cat# 637309 ...
• Long-term hematopoietic stem cells trigger quiescence in Leishmania parasites

  Laura Dirkx, et al., bioRxiv - Microbiology 2023

  Quote: ... parasites were stained with 5-(and 6)-carboxyfluorescein diacetate succinimidyl ester (CFSE; Cell Division Tracker Kit, BioLegend) according to manufacturer’s instructions ...
• Ligand-Displaying E. coli Cells and Minicells for Programmable Delivery of Toxic Payloads via Type IV Secretion Systems

  Yang Grace Li, et al., bioRxiv - Microbiology 2023

  Quote: ... or RNA polymerase (RNAP; BioLegend) and horseradish peroxidase (HRP)-conjugated secondary antibodies (BioRad ...
• Fasciola hepatica GST mu-class suppresses the cytokine storm induced by E. coli-lipopolysaccharide whereas modulates the dynamic of peritoneal macrophages in a mouse model and suppresses the classical activation of macrophages

  Bianca N. Valdes-Fernandez, et al., bioRxiv - Microbiology 2023

  Quote: ... and the Fc receptor of the cells was blocked using an anti-mouse CD16/32 antibody (Fcγ R III/II, Ly-17; BioLegend). After 30 min of incubation at 4°C cells were washed with FACS Buffer (PBS containing 1% FBS ...
• Fasciola hepatica GST mu-class suppresses the cytokine storm induced by E. coli-lipopolysaccharide whereas modulates the dynamic of peritoneal macrophages in a mouse model and suppresses the classical activation of macrophages

  Bianca N. Valdes-Fernandez, et al., bioRxiv - Microbiology 2023

  Quote: PECs isolated from experimental animals were stained and incubated for 30 min with Zombie Violet Fixable Viability Dye (BioLegend) to assess dead cells ...
• Imaging α-synuclein pathologies in animal models and patients with Parkinson’s and related diseases

  Hironobu Endo, et al., bioRxiv - Neuroscience 2023

  Quote: ... and amyloid β (6E10; BioLegend, 803004), and polyclonal antibody against tau phosphorylated at Ser 199 and Thr 202 (pS199/202 ...
• Expression of ALS-PFN1 impairs vesicular degradation in iPSC-derived microglia

  Salome Funes, et al., bioRxiv - Neuroscience 2023

  Quote: ... and TNF-α (430204) from BioLegend according to the manufacturer’s protocol ...
• Transcriptome analyses of mouse cardiac myocytes and non-cardiomyocytes: postmitotic vs. proliferative cells

  Yasuhiro Takenaka, et al., bioRxiv - Physiology 2023

  Quote: ... 7-AAD (BioLegend, #420403) was used to gate live cells ...
• Transcriptome analyses of mouse cardiac myocytes and non-cardiomyocytes: postmitotic vs. proliferative cells

  Yasuhiro Takenaka, et al., bioRxiv - Physiology 2023

  Quote: ... CD45-PE (BioLegend, #103105), CD31-PE (BioLegend ...
• Transcriptome analyses of mouse cardiac myocytes and non-cardiomyocytes: postmitotic vs. proliferative cells

  Yasuhiro Takenaka, et al., bioRxiv - Physiology 2023

  Quote: ... followed by incubation with the antibodies against TER119-PE (BioLegend, #116207), CD45-PE (BioLegend ...
• Vascular Endothelial-derived SPARCL1 Exacerbates Viral Pneumonia Through Pro-Inflammatory Macrophage Activation

  Gan Zhao, et al., bioRxiv - Immunology 2023

  Quote: ... PE/Cyanine7 anti-mouse CD64 (FcγRI) antibody(1:200, Biolegend, X54-5/7.1), Alexa Fluor 488 or PE–conjugated rat anti-mouse CD31 [platelet endothelial cell adhesion molecule 1 (PECAM1)] antibody (1:200 ...
• Vascular Endothelial-derived SPARCL1 Exacerbates Viral Pneumonia Through Pro-Inflammatory Macrophage Activation

  Gan Zhao, et al., bioRxiv - Immunology 2023

  Quote: ... Brilliant Violet 421™ anti-mouse NK-1.1 antibody(1:100; Biolegend, PK136), PE-Cyanine7 CD127 Monoclonal antibody (1:100 ...
• Vascular Endothelial-derived SPARCL1 Exacerbates Viral Pneumonia Through Pro-Inflammatory Macrophage Activation

  Gan Zhao, et al., bioRxiv - Immunology 2023

  Quote: ... Alexa Fluor 700 anti-mouse/human CD45R/B220 antibody(1:50; Biolegend, RA3-6B2), BUV395 anti-mouse CD11b(1:200 ...
• Vascular Endothelial-derived SPARCL1 Exacerbates Viral Pneumonia Through Pro-Inflammatory Macrophage Activation

  Gan Zhao, et al., bioRxiv - Immunology 2023

  Quote: ... Stained cells and “fluorescence minus one” (FMO) controls were then resuspended in SB + 1:1000 DNase + 1:1000 Draq7 (BioLegend, #424001) as a live/dead stain ...
• Vascular Endothelial-derived SPARCL1 Exacerbates Viral Pneumonia Through Pro-Inflammatory Macrophage Activation

  Gan Zhao, et al., bioRxiv - Immunology 2023

  Quote: ... PE/Cyanine5 anti-mouse CD3ε antibody (1:200; Biolegend, 145-2C11), Alexa Fluor 700 anti-mouse/human CD45R/B220 antibody(1:50 ...
• Vascular Endothelial-derived SPARCL1 Exacerbates Viral Pneumonia Through Pro-Inflammatory Macrophage Activation

  Gan Zhao, et al., bioRxiv - Immunology 2023

  Quote: ... slides were probed with primary antibodies (CD31 1:200, Biolegend, MEC13.3 ...
• Vascular Endothelial-derived SPARCL1 Exacerbates Viral Pneumonia Through Pro-Inflammatory Macrophage Activation

  Gan Zhao, et al., bioRxiv - Immunology 2023

  Quote: ... PE anti-mouse Ly-6G antibody(1:200; Biolegend, 1A8), PE/Cyanine7 anti-mouse CD64 (FcγRI ...
• Monomeric amyloid-β inhibits microglial inflammatory activity in the brain via an APP/heterotrimeric G protein-mediated pathway

  Hyo Jun Kwon, Devi Santhosh, Zhen Huang, bioRxiv - Neuroscience 2023

  Quote: ... Supernatants were collected and used for cytokine ELISA assays per manufacturer’s instructions (Biolegend). Total RNAs were prepared from collected cells using Trizol (Invitrogen ...
• Vascular Endothelial-derived SPARCL1 Exacerbates Viral Pneumonia Through Pro-Inflammatory Macrophage Activation

  Gan Zhao, et al., bioRxiv - Immunology 2023

  Quote: ... The cells were then stained with Alexa Fluor® 700 anti-mouse CD206 (MMR) antibody (1:200; Biolegend, C068C2) for 30 min at room temperature ...
• Vascular Endothelial-derived SPARCL1 Exacerbates Viral Pneumonia Through Pro-Inflammatory Macrophage Activation

  Gan Zhao, et al., bioRxiv - Immunology 2023

  Quote: ... Brilliant Violet 711™ anti-mouse/human CD11b antibody (1:200; Biolegend, M1/70), Brilliant Violet 421™ anti-mouse F4/80 antibody(1:100 ...
• Hypoxic memory of tumor intrinsic type I interferon suppression promotes breast cancer metastasis

  Oihana Iriondo, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Antibodies used were CD86 (Biolegend, 105037), F4/80 (Biolegend 123141) ...
• Hypoxic memory of tumor intrinsic type I interferon suppression promotes breast cancer metastasis

  Oihana Iriondo, et al., bioRxiv - Cancer Biology 2023

  Quote: ... CD11b (Biolegend, 101207), CD206 (Biolegend ...
• Hypoxic memory of tumor intrinsic type I interferon suppression promotes breast cancer metastasis

  Oihana Iriondo, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Ly6C (Biolegend, 128012), CD11b (Biolegend ...
• Hypoxic memory of tumor intrinsic type I interferon suppression promotes breast cancer metastasis

  Oihana Iriondo, et al., bioRxiv - Cancer Biology 2023

  Quote: ... CD206 (Biolegend, 141732), Ly6G (BD Biosciences ...
• Alternative splicing of its 5’-UTR limits CD20 mRNA translation and enables resistance to CD20-directed immunotherapies

  Zhiwei Ang, et al., bioRxiv - Immunology 2023

  Quote: ... We stably expressed these plasmids in OCI-Ly8 cells and used fluorescence activated cell sorting (FACS) to enrich for cells that stained negative with PE anti-human CD20 antibodies (clone 2H7, Biolegend). This sorted CD20-negative cell population were the OCI-Ly8 CD20KO cells.
• Transkingdom mechanism of MAMP generation by chitotriosidase (CHIT1) feeds oligomeric chitin from fungal pathogens and allergens into TLR2-mediated innate immune sensing

  Tzu-Hsuan Chang, et al., bioRxiv - Immunology 2023

  Quote: ... supernatants were subjected to ELISA according to manufacturer’s instruction (Biolegend).
• Antibodies Expand the Scope of Angiotensin Receptor Pharmacology

  Meredith A. Skiba, et al., bioRxiv - Pharmacology and Toxicology 2023

  Quote: ... or 1:1000 (v/v) Alexafluor 647 conjugated Anti-Human IgG Fc (Biolegend) (for AT118-L Fc Fusion proteins ...
• Targeting mitochondrial dysfunction using methylene blue or mitoquinone to improve skeletal aging

  Sher Bahadur Poudel, et al., bioRxiv - Pharmacology and Toxicology 2023

  Quote: ... Mononuclear cells were collected and washed with complete medium and 104 cells per well in 96 wells plate were plated in the presence of 40ng/ml M-CSF(Biolegend; cat#576402) and 60ng/ml RANKL (R&D Systems ...