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7601 - 7650 of 10000+ citations since 2019

• Human skeletal muscle organoids model fetal myogenesis and sustain uncommitted PAX7 myogenic progenitors

  Lampros Mavrommatis, et al., bioRxiv - Bioengineering 2023

  Quote: ... Single cell suspension was stained with PE anti-human CD82 Antibody (BioLegend, 342103) in 2%BSA and 2mM EDTA and sorted using FACS sorter (Beckman Coulter ...
• Engineered Wnt7a ligands rescue blood brain barrier and neurobehavioral deficits in a mouse model of COVID-19

  Troy N. Trevino, et al., bioRxiv - Neuroscience 2023

  Quote: ... CD68 (Biolegend FA-11), and Iba1 (Abcam ab178847) ...
• Human skeletal muscle organoids model fetal myogenesis and sustain uncommitted PAX7 myogenic progenitors

  Lampros Mavrommatis, et al., bioRxiv - Bioengineering 2023

  Quote: ... PE anti-human CD184[CXCR4] (Biolegend, clone 12G5) was applied together with the corresponding isotype controls ...
• Maternal diet and gut microbiome composition modulate early life immune development

  Erica T Grant, et al., bioRxiv - Microbiology 2023

  Quote: ... anti-CD8 PE-Cy5 (#100710, clone 53-6.7, 1:700, Biolegend), anti-FoxP3 eF450 (#48-5773-82 ...
• Splenic T follicular helper cells compromise Mycobacterium tuberculosis clearance in aged C57BL/6 mice

  Falak Pahwa, et al., bioRxiv - Immunology 2023

  Quote: ... CXCR5-PE (BioLegend, #145504), ICOS-PerCP/Cy5.5 (BioLegend ...
• Splenic T follicular helper cells compromise Mycobacterium tuberculosis clearance in aged C57BL/6 mice

  Falak Pahwa, et al., bioRxiv - Immunology 2023

  Quote: ... TNF-α (Biolegend, #740154), IL-1β (Biolegend ...
• Splenic T follicular helper cells compromise Mycobacterium tuberculosis clearance in aged C57BL/6 mice

  Falak Pahwa, et al., bioRxiv - Immunology 2023

  Quote: ... CD44-BV510 (BioLegend, #103044), CXCR5-PE (BioLegend ...
• Splenic T follicular helper cells compromise Mycobacterium tuberculosis clearance in aged C57BL/6 mice

  Falak Pahwa, et al., bioRxiv - Immunology 2023

  Quote: ... ICOS-PerCP/Cy5.5 (BioLegend, #313518), PD1-PE Cy7 (BioLegend ...
• Splenic T follicular helper cells compromise Mycobacterium tuberculosis clearance in aged C57BL/6 mice

  Falak Pahwa, et al., bioRxiv - Immunology 2023

  Quote: ... CD8a-BV421 (BioLegend, #100753), CD44-BV510 (BioLegend ...
• Selective targeting of α₄β₇/MAdCAM-1 axis suppresses liver fibrosis by reducing proinflammatory T cell recruitment to the liver

  Biki Gupta, et al., bioRxiv - Immunology 2023

  Quote: ... α4β7/LPAM1(cat. No. 120702, Clone: MECA-367, Biolegend), aSMA (cat ...
• Splenic T follicular helper cells compromise Mycobacterium tuberculosis clearance in aged C57BL/6 mice

  Falak Pahwa, et al., bioRxiv - Immunology 2023

  Quote: ... IL-6 (Biolegend, #740159) and IL-17A (Biolegend ...
• Emergence and suppressive function of Tr1 cells in glomerulonephritis

  Shiwa Soukou-Wargalla, et al., bioRxiv - Immunology 2023

  Quote: Surface staining panel for flow cytometric analysis: CD11b (PE-Cy7, BioLegend, clone M1/70 ...
• Extracellular Delivery of Functional Mitochondria Rescues the Dysfunction of CD4⁺ T Cells in Aging

  Colwyn A. Headley, et al., bioRxiv - Immunology 2023

  Quote: ... Fluorophores were detected using UV laser diode 405 nm (for α-CD4 Pacific Blue; Biolegend) and solid-state lasers 488nm (for Turbo-GFP tagged hexokinase 1 mitochondria (HK-GFP-mito) ...
• Engineered Wnt7a ligands rescue blood brain barrier and neurobehavioral deficits in a mouse model of COVID-19

  Troy N. Trevino, et al., bioRxiv - Neuroscience 2023

  Quote: ... Cells were fixed (Biolegend 421403) and analyzed (Beckman CytoFLEX S).
• Simultaneous selection of nanobodies for accessible epitopes on immune cells in the tumor microenvironment

  Thillai V. Sekar, et al., bioRxiv - Bioengineering 2023

  Quote: ... PE/Cy5-CD25+ and PerCP/Cy5.5-I-A/I-E (Biolegend). FMO controls were prepared for the respective markers.
• Engineered Wnt7a ligands rescue blood brain barrier and neurobehavioral deficits in a mouse model of COVID-19

  Troy N. Trevino, et al., bioRxiv - Neuroscience 2023

  Quote: Cells were sequentially incubated with viability dye (Zombie Green, Biolegend 423111), Fc receptor blockade (Biolegend 101319) ...
• Selective inhibition of fibroblast-specific Domain Discoidin Receptor 1 (DDR1) reduces collagen deposition and modulates fibroblast-specific cytokine release within the breast microenvironment

  Daniel E.C. Fein, et al., bioRxiv - Cell Biology 2023

  Quote: ... CD19-APC (BioLegend 115511; 1:800), CD8-BV711 (BioLegend 100747 ...
• Selective inhibition of fibroblast-specific Domain Discoidin Receptor 1 (DDR1) reduces collagen deposition and modulates fibroblast-specific cytokine release within the breast microenvironment

  Daniel E.C. Fein, et al., bioRxiv - Cell Biology 2023

  Quote: ... CD4-APC-Cy7 (BioLegend 100413; 1:400). Propidium iodide (1:5000 ...
• LAG3 regulates antibody responses in a murine model of kidney transplantation

  Michael Nicosia, et al., bioRxiv - Immunology 2023

  Quote: ... and the following antibodies: rabbit monoclonal anti-mouse/human Mac-2 (clone M3/M8; Biolegend #125401), rabbit monoclonal anti-mouse CD4 (clone EPR19514 ...
• Hypoxic memory of tumor intrinsic type I interferon suppression promotes breast cancer metastasis

  Oihana Iriondo, et al., bioRxiv - Cancer Biology 2023

  Quote: ... BRx68 cells grown in different conditions were stained with HLA-ABC antibody (Biolegend, 311410). The myeloid-derived suppressor cells (MDSC ...
• The Prp19C subunits Cwc15 and Syf2 function in TREX occupancy and transcription elongation

  Laura Henke-Schulz, Rashmi Minocha, Katja Sträßer, bioRxiv - Biochemistry 2023

  Quote: ... or the CTD of Rpb1 (8WG16, Biolegend).
• The Prp19C subunits Cwc15 and Syf2 function in TREX occupancy and transcription elongation

  Laura Henke-Schulz, Rashmi Minocha, Katja Sträßer, bioRxiv - Biochemistry 2023

  Quote: ... 4 μl of the monoclonal antibody 8WG16 (Biolegend) were added for 1.5 h at RT followed by 1 h incubation with Protein G Dynabeads ...
• Chemokine receptor 5 signaling in PFC mediates stress susceptibility in female mice

  Hsiao-Yun Lin, et al., bioRxiv - Animal Behavior and Cognition 2023

  Quote: ... CD31 (1:300, 102501, Biolegend). Sections were then washed and incubated with secondary antibody (1:400 ...
• Reprogramming of breast tumor-associated macrophages with modulation of arginine metabolism

  Veani Fernando, et al., bioRxiv - Cancer Biology 2023

  Quote: ... CD163 (BioLegend, Cat. No. 333609), CD206 (BioLegend ...
• Reprogramming of breast tumor-associated macrophages with modulation of arginine metabolism

  Veani Fernando, et al., bioRxiv - Cancer Biology 2023

  Quote: ... CD206 (BioLegend, Cat. No. 321109), anti-mouse F4/80 (BioLegend ...
• Reprogramming of breast tumor-associated macrophages with modulation of arginine metabolism

  Veani Fernando, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-mouse CD80 (BioLegend, Cat. No. 104706), anti-mouse CD163 (BioLegend ...
• Reprogramming of breast tumor-associated macrophages with modulation of arginine metabolism

  Veani Fernando, et al., bioRxiv - Cancer Biology 2023

  Quote: ... CD40 (BioLegend, Cat. No. 334305), CD80 (BioLegend ...
• Reprogramming of breast tumor-associated macrophages with modulation of arginine metabolism

  Veani Fernando, et al., bioRxiv - Cancer Biology 2023

  Quote: ... The antibodies used are as follows: CD68 (BioLegend, Cat. No. 333821), CD40 (BioLegend ...
• Reprogramming of breast tumor-associated macrophages with modulation of arginine metabolism

  Veani Fernando, et al., bioRxiv - Cancer Biology 2023

  Quote: ... CD80 (BioLegend, Cat. No. 305205), CD163 (BioLegend ...
• Reprogramming of breast tumor-associated macrophages with modulation of arginine metabolism

  Veani Fernando, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-mouse IL12 (BioLegend, Cat. No. 505206), anti-mouse IL10 (BioLegend ...
• Reprogramming of breast tumor-associated macrophages with modulation of arginine metabolism

  Veani Fernando, et al., bioRxiv - Cancer Biology 2023

  Quote: ... 1µg/ml anti-human CD3 antibody (BioLegend, Clone OKT3, Cat. No. 317326) and 1µg/ml anti-human CD28 antibody ((BioLegend ...
• Reprogramming of breast tumor-associated macrophages with modulation of arginine metabolism

  Veani Fernando, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-mouse CD163 (BioLegend, Cat. No. 155306), anti-mouse NK1.1 (ThermoFisher ...
• Reprogramming of breast tumor-associated macrophages with modulation of arginine metabolism

  Veani Fernando, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and 1µg/ml anti-human CD28 antibody ((BioLegend, Clone CD28.2, Cat. No. 302934). The cells were maintained in a 37°C ...
• Glutamine antagonist DRP-104 suppresses tumor growth and enhances response to checkpoint blockade in KEAP1 mutant lung cancer

  Ray Pillai, et al., bioRxiv - Cancer Biology 2023

  Quote: Mice were sedated with ketamine and xylazine and then were injected with 2 ug of APC anti-CD45 (2 ug per mouse diluted in 100 uL PBS, Biolegend 30-F11) retro-orbitally ...
• Glutamine antagonist DRP-104 suppresses tumor growth and enhances response to checkpoint blockade in KEAP1 mutant lung cancer

  Ray Pillai, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Cells were then stained with live dead staining (Zombie UV fixable viability dye, Biolegend #423107) and PeCy7 anti-CD45 (see flow cytometry methods section for staining protocol).
• Glutamine antagonist DRP-104 suppresses tumor growth and enhances response to checkpoint blockade in KEAP1 mutant lung cancer

  Ray Pillai, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Live/dead staining was initially performed per protocol (Zombie UV fixable viability dye, Biolegend 423107). Cells were then blocked with Fc block (2.4G2 ...
• Functional precision oncology for follicular lymphoma with patient-derived xenograft in avian embryos

  Manon Zala, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Cells were first blocked with 2% mouse serum (eBioscience San Diego, California) and Human Fc Block (Biolegend San Diego, California) for 10 minutes on ice ...
• RNA binding proteins and glycoRNAs form domains on the cell surface for cell penetrating peptide entry

  Jonathan Perr, et al., bioRxiv - Cell Biology 2023

  Quote: ... primary conjugated antibodies (AF647) were used directly for cell labeling and included mouse IgG1,k isotype (BioLegend, 400130), mouse IgG2b isotype (BioLegend ...
• Various Cell Types in the Bone Marrow sustain Primary B-Cell Precursor Acute Lymphoblastic Leukemia

  Mandy W. E. Smeets, et al., bioRxiv - Cancer Biology 2023

  Quote: ... FITC Annexin V (Biolegend), and propidium iodide (PI ...
• Chemogenetic inhibition of IST1-CHMP1B interaction impairs endosomal recycling and promotes unconventional LC3 lipidation at stalled endosomes

  Anastasia Knyazeva, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-ALIX (1:1000, #634502, BioLegend), anti-TSG101 (1:1000 ...
• Lineage tracing of newly accrued nuclei in skeletal myofibers uncovers distinct transcripts and interplay between nuclear populations

  Chengyi Sun, et al., bioRxiv - Cell Biology 2023

  Quote: ... and Ter119 for negative selection (all PE-conjugated, Biolegend). The labeling efficacy of Pax7rtTA ...
• Lineage tracing of newly accrued nuclei in skeletal myofibers uncovers distinct transcripts and interplay between nuclear populations

  Chengyi Sun, et al., bioRxiv - Cell Biology 2023

  Quote: ... The resultant mononuclear cell suspensions were treated with antibodies against VCAM1 (Biolegend) for APC detection of myogenic cells ...
• EZH2 inhibition promotes tumor immunogenicity in lung squamous cell carcinomas

  Tanner J. DuCote, et al., bioRxiv - Cancer Biology 2023

  Quote: ... they were then stained with EpCAM-PECy7 (BioLegend #118216) and CD31-APC (Biolegend Cat# 102510) ...
• A genome-wide CRISPR screen in human prostate cancer cells reveals drivers of macrophage-mediated cell killing and positions AR as a tumor-intrinsic immunomodulator

  Anniek Zaalberg, et al., bioRxiv - Cancer Biology 2023

  Quote: ... cells were labeled with an antibody cocktail containing Anti-CD14-PE/Cy7 (Clone 63D3, 367111, BioLegend, 1:100), Anti-CD80-APC (Clone 2D10 ...
• Soluble CTLA-4 raises the threshold for T-cell activation and modulates anti-tumour immunity

  Paul T. Kennedy, et al., bioRxiv - Cancer Biology 2023

  Quote: ... PBMCs and HeLa target cells were co-cultured in u-bottom 96 well plates at a range of T:E ratios (indicated in figure legends) T cells were stimulated with 10 µg/mL plate-immobilised anti-CD3 (OKT3, BioLegend) and cultured for 4 days prior to staining with anti-CD8-APC (SK1 ...
• Soluble CTLA-4 raises the threshold for T-cell activation and modulates anti-tumour immunity

  Paul T. Kennedy, et al., bioRxiv - Cancer Biology 2023

  Quote: ... cells were harvested and stained with anti-mouse-CD8α (BioLegend) for analysis of T cell proliferation by flow cytometry as above.
• A genome-wide CRISPR screen in human prostate cancer cells reveals drivers of macrophage-mediated cell killing and positions AR as a tumor-intrinsic immunomodulator

  Anniek Zaalberg, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Anti-CD86-FITC (Clone BU63, 374203, BioLegend, 1:100) for 45 minutes on ice ...
• Soluble CTLA-4 raises the threshold for T-cell activation and modulates anti-tumour immunity

  Paul T. Kennedy, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and supplemented with sCTLA-4 conditioned media or murine CTLA-4-Fc fusion protein (10 µg/mL, BioLegend). After 4 days ...
• Soluble CTLA-4 raises the threshold for T-cell activation and modulates anti-tumour immunity

  Paul T. Kennedy, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and cultured for 4 days prior to staining with anti-CD8-APC (SK1, BioLegend) and proliferation measured by flow cytometry ...
• EZH2 inhibition promotes tumor immunogenicity in lung squamous cell carcinomas

  Tanner J. DuCote, et al., bioRxiv - Cancer Biology 2023

  Quote: ... H-2Kd/H-2Dd-Alexa Fluor® 647 (BioLegend #114712), NGFR (Cell Signaling #8238S) ...