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Citations for BioLegend :
7051 - 7100 of 7138 citations for Primary Human Melanocyte Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... adhesive bone marrow cells were obtained using the above protocol and selected cell populations were sorted as follows: Live/Dead staining was performed using zombie NIR (Biolegend, Cat. 423106) for 30min at 4°C ...
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bioRxiv - Immunology 2023Quote: Up to two million cells from each sample were stained with 2µl Totalseq A hashtag antibodies (Biolegend, see supplementary table for details) on ice for 20 minutes ...
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bioRxiv - Immunology 2024Quote: ... Cells were harvested and stained for 30min at 4°C with the following antibodies: anti-CD25 BV785 (Biolegend, Cat: 356139, 1:50), anti-CD127 PE (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... stably surface GFP-expressing T2 cells were identified with the GFP marker or with their CD19 and CD20 expressions via PE/Cy7-CD19 (Biolegend, cat# 302216) and PE/Cy7-CD20 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... cells were first stained with AViD Live/Dead Fixable Yellow Dead Cell Staining Reagent and subsequently stained with PE anti-CD3 (Biolegend, clone UCHT1), PE/Cy7 anti-CD56 (Biolegend ...
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bioRxiv - Neuroscience 2021Quote: ... The cell pellets were washed once with 15 mL PBS and resuspended in 1 mL of red blood cell (RBC) lysis buffer (BioLegend, San Diego, CA) for 2 min at room temperature to lyse RBCs ...
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bioRxiv - Genomics 2020Quote: ... Cells were blocked with 1% BSA/PBS for 30 minutes and then incubated with antibodies for HA.11 (BioLegend MMS-101P, 1:2000) and BIP (Abcam ab21685 ...
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bioRxiv - Immunology 2019Quote: ... After isolation cells were treated with Fc receptor blocking solution and stained with anti-ICAM-1-PE (1 μg/ml, HA58; Biolegend, San Diego, USA), anti-VCAM-1-BV711 (0.5 μg/ml ...
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bioRxiv - Microbiology 2021Quote: ... 20 μl cells were removed for flow analysis and remaining cells were incubated for 30 min on ice with 1 μg each of TotalSeq™-A0441 anti-mouse CD71 Antibody (Biolegend, Cat# 113824) and TotalSeq™-A0073 anti-mouse/human CD44 Antibody (Biolegend ...
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bioRxiv - Bioengineering 2021Quote: P14 CD8+ T cells were isolated as described above and incubated with DiO-labeled Db-GP33 liposomes and anti-mouse CD8 (Biolegend, Clone: 53-6.7) at 4°C or 37°C for 30 min ...
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bioRxiv - Bioengineering 2021Quote: ... 22 and 28 before differentiation of mast cells was assessed by flow cytometry with antibody markers for CD117 APC (2B8, Biolegend, San Diego, CA) and FcεRI PE (MAR-1 ...
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bioRxiv - Microbiology 2020Quote: ... After 48h of co-infection the cells were harvested and processed as above using an anti-MHC-II FITC-conjugated antibody (Biolegend San Diego, CA). Treatment with PMA (50 ng/ml ...
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bioRxiv - Microbiology 2021Quote: ... 100 µl containing 1 × 106 cells in PBS were incubated with yellow Zombie viability dye (1:1000 dilution, Cat. No 423104, Biolegend, San Diego, CA) for 15 minutes at room temperature followed by washing in FACS buffer ...
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bioRxiv - Immunology 2021Quote: Freshly prepared cardiac single-cell suspensions from healthy and myocarditic hearts were subjected to surface staining with Annexin V (Biolegend, San Diego, CA) and propidium iodide (PI ...
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bioRxiv - Cancer Biology 2020Quote: ... coated with intercellular adhesion molecule (ICAM-1, ICAM-2) and vascular cell adhesion molecule (VCAM-1) antibodies conjugated to Alexa Fluor 680 (BioLegend, San Diego, CA). Sorted cells were cultured in Endothelial Growth Media (EGM-2 ...
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bioRxiv - Immunology 2022Quote: Whole PBMCs were seeded at 1-2×10^6 cells per well in 48-well plates in the presence of 200ng/ml soluble anti-CD3 (OKT3 clone, Biolegend, San Diego, CA) and 10ng/ml IL-2 (Peprotech ...
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bioRxiv - Physiology 2022Quote: Apoptotic cells were analyzed by flow cytometry using the FITC Annexin V Apoptosis Detection Kit with propidium iodide (BioLegend, San Diego, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... single cell suspensions from mouse spleens were stained with fluorescein isothiocyanate (FITC)-conjugated anti-Ly-6A/E (Sca-1) mAb (Biolegend; San Diego, CA). To analyze erythrocyte maturation ...
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bioRxiv - Genomics 2022Quote: ... Bone marrow mononuclear cells were stained for 30 minutes on ice with the following antibodies: anti-CD19(APC/Cy7) (clone HIB19; Biolegend; Cat. num. 302217) in a 1:20 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... and were stained at 20 μl of FACS buffer per 1×106 cells containing the first set of following antibodies (all from Biolegend, unless specified otherwise): biotinylated hematopoietic lineage antibodies against NK1.1 (clone PK136) ...
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bioRxiv - Immunology 2020Quote: ... DCs were isolated from spleens and lymph nodes of C57BL/6 mice using a MojoSort™ Mouse Pan Dendritic Cell Isolation Kit (BioLegend cat. 480097). DCs were cultured with supernatants of necrotic keratinocytes generated by an overnight culture of UV-irradiated (2 J/m2/s for 40 min ...
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bioRxiv - Immunology 2020Quote: Concentrations of IL-1β and IL-6 in cell culture supernatants or blood serum were measured using cytokine-specific ELISAs (BioLegend ELISA Max kits), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... the cells were incubated for 15 minutes on ice with an Alexa Fluor 647-anti-IgG antibody (clone M1301G05, Biolegend, San Diego, CA). Control experiments were performed by skipping the incubation with TIM-4 ...
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bioRxiv - Immunology 2022Quote: ... Sorted naïve 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11, BioLegend, San Diego, CA) and soluble 1 μg/mL anti-mouse CD28 mAb (clone 37.51 ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were washed and resuspended in 100 μl cold differentiation media with 2μl anti-MHCI (Biolegend, anti-HLA-ABC, clone W6/32, 311406). After a 30min incubation at 4□C ...
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bioRxiv - Physiology 2019Quote: ... cells were stained with Tag-It Violet™ Proliferation and Cell Tracking Dye according to the manufacturer’s instructions (Catalog #425101, Biolegend, San Diego, CA). Briefly ...
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bioRxiv - Biochemistry 2021Quote: Quadruplicate samples of WT HEK293T and CCT5-FLAG tagged cells were lysed and purified using anti-FLAG affinity matrix (Biolegend cat no. 651501) using 20mM HEPES pH 7.5 and 100mM NaCl wash buffer and 200mM Glycine/150mM NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... CD25 surface expression were further confirmed by staining the transduced HEK293T cells with anti-CD25 Brilliant Violet 605 antibody (BioLegend, Clone BC96, #302632). Fluorescence was monitored by flow cytometry and was compared to non-transduced HEK293T cells (data not shown) ...
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bioRxiv - Microbiology 2019Quote: ... as assessed by measuring the percentage of CD3+/CD4+ cells via flow cytometry with FITC-anti-CD3 (Biolegend, San Diego, CA, catalogue #317306) and APC-anti-CD4 (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: ... were prepared from mT/mG mice as described with modifications.55 Ten million bone marrow cells/well were plated overnight in medium containing 20ng/ml murine GM-CSF(BioLegend, San Diego, CA), replenishing the medium daily for three days ...
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bioRxiv - Immunology 2021Quote: BMDMs were generated by culturing total BM cells for 7 days in complete RPMI supplemented with 10 ng/mL recombinant mouse M-CSF (BioLegend, Cat# 576406M-CSF). C8-S cells (ATCC ...
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bioRxiv - Cell Biology 2022Quote: ... and the cells were resuspended in 200 μL biding buffer with 2μL 7-AAD and 2μL annexin V-FITC (Biolegend Inc, San Diego, CA, USA). The plate was incubated for 15 min in darkness and measured in the flow cytometer FC 500 (Beckman-Coulter ...
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bioRxiv - Cancer Biology 2022Quote: ... a sequential gating strategy was used to analyze cancer cells by staining with the following FACS antibodies (Figure S3F): CD45-Pacific Blue (BioLegend 103126, 1:100), CD31-Pacific Blue (BioLegend 102422 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then stained for viability using Zombie NIR™ fixable viability kit for 15 min at room temperature (Cat # BioLegend, CA, USA), followed by fluorescently labeled high- or low-molecular weight HA (2.5mg/ml in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... obtained single cell suspensions were stained with the fixable viability Zombie Aqua™ dye according with the manufacturer instructions (#423101; BioLegend, 1:500).
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bioRxiv - Immunology 2022Quote: ... T cells were added to anti-CD3 coated wells at 106 cells/mL in the presence of 1 μg/mL anti-CD28 (Biolegend, LEAF purified, 37.51), 100 U/ml IL-2 (gift from K ...
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bioRxiv - Bioengineering 2024Quote: T cells were activated by incubating PBMCs with anti-CD3 (OKT3, Tonbo/Cytek, San Diego, CA) and anti-CD28 (302934, Biolegend, San Diego, CA) antibodies in PBS at 37°C for 3 to 5 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... Filtered single cell suspensions were blocked (anti-mouse CD16 / CD32) and stained for live/dead discrimination (Zombie Aqua, BioLegend, or EMA, Thermo Fisher) prior to staining with fluorophore-conjugated antibodies (30 min ...
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bioRxiv - Cell Biology 2024Quote: ... naïve CD4 and CD8 T-cells were isolated from syngeneic cryopreserved PBMC aliquots using the MojoSort magnetic negative selection kits (BioLegend, 480041 and 480045). Naïve T-cells were incubated in complete medium supplemented with 10 IU/mL recombinant human IL-2 (R&D Systems ...
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bioRxiv - Physiology 2024Quote: IL-1β concentrations in the conditioned medium from murine splenic cells were measured by ELISA kits (mouse IL-1β, 432604, BioLegend, San Diego, USA) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: NK cells were enriched from splenocytes from WT or Pvrig KO C57BL/6 mice by using the MojoSort™ Mouse NK Cell Isolation Kit (Biolegend, cat. 480049). Enriched NK cells were cultured and stimulated in vitro in RPMI-1640 Medium (ATCC ...
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bioRxiv - Immunology 2024Quote: ... Cell samples were stained with the following antibodies and stains (in multiple sets to prevent fluorophore overlap): B220-FITC (BioLegend, clone RA3-6B2), CD11c-PacBlue (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... 500 uL cell suspension was added to 12x75mm polystyrene tubes and incubated with 100 uL TruStain FcX™ (BioLegend, San Diego, CA, USA) on ice for 20 min ...
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bioRxiv - Immunology 2023Quote: ... The whole cell pellet from the entire organ was stained with a panel of directly conjugated antibodies supplied by Biolegend (San Diego, US) or eBioscience (San Diego ...
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bioRxiv - Immunology 2023Quote: ... tumor cell suspensions were incubated 10 min at RT with Fc block (Leico; C381) and a viability dye (Biolegend; Zombie Aqua (ref # 423102) or Zombie NIR (ref # 423106) ...
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bioRxiv - Microbiology 2023Quote: ... 2 million splenocytes were resuspended in 50 µl of fluorescence-activated cell sorter (FACS) buffer (PBS with 2% fetal bovine serum) and blocked with TruStain fcX (clone 93; BioLegend, San Diego, CA). The cells were washed and stained to identify B cell subsets with fluorophore-conjugated antibodies against CD45.2 (clone 104) ...
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bioRxiv - Biophysics 2023Quote: ... in combination with a strong forwards and side scatter (for neutrophils) or a second cell-type specific surface marker (CD3 (Biolegend, cat. no. 300408) for T cells ...
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bioRxiv - Cancer Biology 2023Quote: ... NKG2D and DNAM-1 surface receptors on NK cells were blocked utilizing 25 μg/ml of purified anti-mouse NKG2D (Biolegend, clone CX5, 130202), purified anti-mouse DNAM-1 (Biolegend ...
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bioRxiv - Physiology 2023Quote: CD8+ cells were isolated from the spleens of four 8-week-old diabetic female NY8.3 NOD mice using a MojoSort Mouse CD8 T-cell isolation kit (Cat# 480043, BioLegend, San Diego, CA, USA) and MojoSort buffer (Cat# 480017 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were pretreated with 100 U/mL IFN-γ for 24h and stained with 1:1000 H2kb-SIINFEKL Ab (BioLegend,Clone:25-D1.16). GFP-expressing cell lines were used for GFP compensation ...