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Citations for BioLegend :
6951 - 7000 of 7138 citations for Primary Human Melanocyte Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... CAR-transduced (thus EGFRt+) T cells were detected by staining with anti-EGFR antibody (clone AY13, Biolegend, San Diego, CA, USA) and scFv-Linker (custom made ...
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bioRxiv - Bioengineering 2023Quote: ... Purity of isolated T cell fractions was verified by staining with fluorophore-conjugated antibodies (all from Biolegend, San Diego, CA, USA) and 7-AAD staining solution (130-111-568 ...
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bioRxiv - Cell Biology 2022Quote: ... Twenty four hours after irradiation cells were trypsinized and stained using the FITC Annexin V Apoptosis Detection Kit with PI (Biolegend, CA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The reaction was stopped by centrifugation at 4°C followed by staining of cell surface LAMP-1 (CD107a) using FITC-coupled anti-LAMP-1 antibody (clone 1D4B, BioLegend, #121605) for 20 minutes at 4°C in the dark ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... was used to mark single cells of DMSO treated mice and TotalSeq™-B0304 anti-mouse Hashtag 4 Antibody (Clone M1/42; 30-F11; 155837; BioLegend) was used for aortic cells of TRAF-STOP treated mice ...
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bioRxiv - Immunology 2023Quote: ... All labelling was carried out at room temperature (RT) for 10mins protected from light with antibodies diluted in cell staining buffer (Biolegend; 420201). For AnnexinV staining ...
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bioRxiv - Cancer Biology 2023Quote: Cells were stimulated with IFNγ for 4h and the trypizinized cells were immunostained with VCAM1 antibody (1:100 dilution, BV421; BioLegend #305815). eBioscience™ Fixable Viability Dye eFluor™ 780 was used for discriminating dead cells ...
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bioRxiv - Developmental Biology 2023Quote: ... Then cells were again washed and followed by an incubation with anti-mouse IgG coupled to PE-Cy7 (Biolegend ref 406613) at a 1/200 dilution in BD wash buffer during 20 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... single cell suspensions of samples incubated with myeloid antibody cocktail were washed and resuspended in PBS for flow cytometry run whereas lymphoid antibody cocktail-stained cells were incubated overnight with 1 mL perm buffer (Biolegend; 421402) containing 10 μL of anti-FoxP3 (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... cell suspensions were stained for 30 min at 4°C in the dark with CD45-BV605 (1:50, BioLegend #304042, RRID:AB_2562106), ITGA6-PeCy7 (1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed in FACS buffer three times and fixed overnight in 200 µL True Nuclear fixation buffer (Biolegend #73158, #71360) at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Following incubation on ice for 30 min., cells were pelleted, washed, and resuspended in PBS/BSA containing streptavidin-APC (1:2000 dilution in PBS/BSA, (BioLegend #405207)) and incubated for 30 min on ice prior to washing ...
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bioRxiv - Physiology 2023Quote: ... Cisplatin is washed off and non-specific binding was blocked with a 10-minute staining of cells with Fc Block (Biolegend; 1µg). Cells were stained with 0.5µg of cell surface metal-conjugated antibodies for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... 1.5 million cells were washed twice with PBS prior to 15 min of staining with the Zombie NIRTM Fixable Viability dye (BioLegend – 423105), followed by 2 washes with FACS Buffer (PBS ...
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bioRxiv - Neuroscience 2024Quote: ... CD11b+CD45.2+CX3CR1+ microglia were sorted into a pre-cooled 5ml low-protein binding falcon tube (fisher scientific; Cat#: 13-864-407) containing 0.5ml cell staining buffer (BioLegend, Cat#: 420201). Walls of the tubes were washed and cells pelleted at 300g ...
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bioRxiv - Neuroscience 2023Quote: Endothelial cells isolated from brain from SARS-CoV-2 infected mice were stained with fixable viability dye (Zombie Green, Biolegend 423111) and blocked with Fc receptor blockade (anti-mouse CD16/32 ...
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bioRxiv - Immunology 2023Quote: ... 6 x 106 cells were stained with the appropriate cocktail of cell-surface conjugated antibodies in 200µL FWB with the fixable viability dye Zombie Red (BioLegend; 1:1000) for 30 minutes on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... maximum cellular cytokine release per well was determined by the addition of 0.2 μL Cell Activation Cocktail (without Brefeldin A) (BioLegend Cat. #423302) per 100 μL cell solution ...
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bioRxiv - Cancer Biology 2023Quote: ... About 1*106 live cells were then blocked with TruStain FcXTM anti-mouse CD16/32 antibody (Clone 93; Biolegend; Cat #101320) on ice for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... and incubated in 100 μl of Cell Staining Buffer with TruStain FcX™ (anti-mouse CD16/32) Antibody (1:100; 101320, Biolegend) and True-Stain Monocyte Blocker™ (1:20 ...
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bioRxiv - Immunology 2023Quote: ... cells were washed three times (300g, 5 min, 4⁰C) in cell staining buffer and kept in FluoroFix™ Buffer (422101, Biolegend) prior to run in a BD FACSymphony™ A5 Cell Analyzer (BD Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed three times (300g, 5 min, 4⁰C) in cell staining buffer and kept in FluoroFix™ Buffer (422101, Biolegend) before run in a BD FACSymphony™ A5 Cell Analyzer (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... CD4 T cells were isolated from the spleen and LNs of indicated naïve OT-II strains with Mouse CD4 T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) and labeled with 1.0 µM CFSE (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies to the following proteins were used at the indicated concentrations: Ubq (1:5000, P4D1, Cell Signaling; 1:5000, P4G7-HRP, BioLegend), Actin (1:10,000 ...
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bioRxiv - Biophysics 2023Quote: ... then cells were stained overnight with DAPI and an Alexa Fluor 647-conjugated anti-cytochrome c antibody (Biolegend, clone 6H2.B4). Stained cells were analyzed using an Attune NxT flow cytometer ...
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bioRxiv - Immunology 2023Quote: ... LN single cell suspensions were stimulated in a 96-well round-bottom plate with Cell Stimulation Cocktail containing PMA and ionomycin (eBioscience 00-4970-03, Thermo Fisher or 423302, BioLegend) at 37 °C for 1 hour ...
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bioRxiv - Bioengineering 2024Quote: ... cells were washed and stained with an Allophycocyanin (APC) labeled secondary antibody (Donkey anti-rabbit IgG DyLight™ 649, Biolegend 406406) for 30-45 minutes at room temperature in 2% normal donkey serum ...
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bioRxiv - Immunology 2024Quote: Cells were stained with 20 µL of surface antibody cocktail with Zombie NIR fixable viability kit (1:400-1000, Biolegend, 423105) in PBS for 20 minutes on ice ...
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bioRxiv - Developmental Biology 2024Quote: Single-cell suspensions were obtained at day 2.5 of differentiation Cells were first labeled with Zombie NIR fixable viability dye (BioLegend, 1:500) for the exclusion of dead cells ...
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bioRxiv - Immunology 2023Quote: ... CD3+ T cells were isolated from spleens of 6-to-8-week-old OT-II mice by MojoSort mouse CD3+ T cell isolation kit (Biolegend 480024). Stimulated FLS were washed with PBS and co-cultured with CD3+ T cells at a concentration of 5-10×105 cells/mL in 10% RPMI for 5 days with either the cognate OVA323-339 antigen (2μg/mL ...
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bioRxiv - Immunology 2024Quote: Production of IFNγ and IL-10 from T cell culture supernatant was measured using Legend Max ELISA Kit Mouse IFNγ (BioLegend, 430807) and Mouse IL-10 (BioLegend ...
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bioRxiv - Microbiology 2024Quote: ... each sample was counterstained with the desired concentration (0.5ul/1 × 106 cells) of 7AAD viability staining solution (Biolegend; Cat. No. 420404) before analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... primary AML blast or CD34+ cells purified from CMML patients or healthy donors were suspended in Annexin V Binding buffer (BioLegend #422201) and incubated with anti-Annexin V antibody (BioLegend #640920 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were pelleted by centrifugation (2,000 rpm 5 min) and then treated with 1 mL 1X red blood cell (RBC) Lysis Buffer (Biolegend; cat# NC9067514) to lyse red blood cells ...
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bioRxiv - Cell Biology 2024Quote: ... Cell pellets were gently resuspended in 100 µl FACS buffer and stained with antibodies (CD38-APC, Clone: HIT2 (Biolegend, 1:100), CD45RA-PE-Cy7 ...
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bioRxiv - Cancer Biology 2024Quote: ... and the cells were subsequently stained overnight with DAPI and an Alexa Fluor 647-conjugated anti-cytochrome c antibody (Biolegend, 612310) at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... The cell suspension was filtered through a 70 μm cell strainer and seeded into a 150 mm petri dish with 40 ng/ml of m-CSF (BioLegend, 576406) for 7 days ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were treated with CD16/CD32 blocking antibody (Ultra-LEAF Purified Anti-mouse CD16/32 Antibody, Biolegend, San Diego, CA, USA), to prevent phagocytosis ...
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bioRxiv - Evolutionary Biology 2024Quote: Dissociated cells derived from forebrain and connective tissue were stained with APC-anti-mouse CD45 antibody (Biolegend, San Diego, CA; 103112), PE-Cy7-anti rat CD45 (Biolegend ...
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bioRxiv - Bioengineering 2024Quote: ... supernatants were drawn off the cells and analyzed for cytokine content (i.e., IL-2, IFN-γ, and TNF-α) by ELISA kits (Biolegend).
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bioRxiv - Cancer Biology 2024Quote: ... the cells were washed with DPBS three times and stained with 1 µg/mL Streptavidin-Alexa Fluor 647 (AF647) (BioLegend, #405237) for another 30 min ...
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bioRxiv - Immunology 2024Quote: ... single cell suspension was incubated with the cell hashtag antibodies (unique DNA barcodes listed in Supplementary Table 2) and the cocktail of 119 unique cell surface antibodies (Biolegend, #199901), as per manufacturer’s protocol.
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bioRxiv - Cancer Biology 2021Quote: ... The cells and the culture media were collected and stained with annexin V-FITC mAb (BioLegend, San Diego, CA, Cat. No. 640906) in binding buffer (10 mM HEPES ...
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bioRxiv - Cell Biology 2020Quote: ... Around 900 μl of plasma from each mouse was used to stain for T cell (1:150, APC-CD3e/clone 145-2C11, Biolegend Cat. 100312), B cell (1:300 ...
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bioRxiv - Immunology 2021Quote: The IFN-β levels in cell supernatants and mouse sera were measured with ELISA kits purchased from BioLegend (San Diego, CA, USA).
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bioRxiv - Molecular Biology 2021Quote: ... the cells were incubated for 10 min at RT in 1.0 μg anti-mouse CD16/32 antibody (BioLegend, San Diego, CA, USA) containing 500 μl FACS buffer for Fc receptor blocking ...
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bioRxiv - Immunology 2020Quote: ... CAFs were co-cultured overnight with CD4+ T cells at a 1:1 ratio in the presence of pan-HLA antibody (5μg/ml, Biolegend, Cat. No.361702) or GC1q R antibody (10μg/ml ...
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bioRxiv - Immunology 2019Quote: After isolation cells were treated with Fc receptor blocking solution and stained with anti-CD14-APC (2 μg/ml, 61D3; Biolegend, San Diego, USA), anti-CD41-PE (2 μg/ml ...
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bioRxiv - Immunology 2019Quote: PBMCs were seeded in a 24 well plate at 2 × 106 cells/well in RPMI-1640 with anti-CD107a antibody (Biolegend, 1:200). Then ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then stained with 100 μL of their respective stains (αCD4-FITC, αCD8-FITC, αCD19-FITC, αCD14-APC; Biolegend, https://www.biolegend.com) at a concentration of 1μg/mL for 30 min at 4°C ...