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Citations for BioLegend :
5651 - 5700 of 7138 citations for Primary Human Melanocyte Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... the cells were washed with PBS and stained with fixable Zombie NIR dye (Biolegend, 1:500) for 20 min at RT ...
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bioRxiv - Immunology 2021Quote: ... cells were washed twice using 150 µl/well intracellular-staining Perm-Wash buffer (BioLegend, cat. 421002) diluted 1:10 in PBS ...
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bioRxiv - Immunology 2021Quote: ... cells were permeabilized and labeled with the described cocktail plus APC-IFN-γ (BioLegend Cat# 505810) and PE-IL-17A (BioLegend Cat# 506903) ...
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bioRxiv - Immunology 2021Quote: ... cells were first stained with biotin-conjugated anti-mouse antibodies against CD3 (BioLegend, clone 145-2C11), CD4 (BioLegend ...
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bioRxiv - Microbiology 2019Quote: ... Paraformaldehyde-fixed cells were stained using anti-HLA-DR APC conjugate (Biolegend, Clone L243, 1:50). Latently infected CD14+ monocytes were fixed with 1% paraformaldehyde and stained using anti-HLA-DR Pacific blue conjugate (Biolegend ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were incubated for 10 min using unconjugated anti-mouse CD16/32 antibody (Biolegend, C93, #101301) at 4°C prior to labelling with specific fluorochrome-labelled antibodies ...
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bioRxiv - Developmental Biology 2020Quote: ... Cell pellets were resuspended in Zombie Violet Live-Dead Stain (1:500 in PBS; BioLegend, 423113) and incubated for 15 min in the dark ...
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bioRxiv - Immunology 2022Quote: ... All cell lines showed similar expression of HLA-A2 by flow cytometry (clone BB7.2, Biolegend 343306).
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bioRxiv - Immunology 2021Quote: ... Cell sorts were performed with MojoSort Mouse Pan B Isolation Kit II (BioLegend, San Diego, CA) for CD19+ cells and with Miltenyi’s magnetic-activated cell sorting (MACS ...
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bioRxiv - Genomics 2022Quote: ... THP1 cells were first treated with FcX-blocking buffer (BioLegend #422302; 10 min at room temperature), before staining cells with a pool of 22 TotalSeq-A antibodies (Supplementary Table 3) ...
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bioRxiv - Immunology 2022Quote: ... cells were stained in PBS containing 2% (w/v) BSA and the appropriate antibodies from Biolegend. For analyzing intracellular cytokine IFN-γ and IL-17A ...
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bioRxiv - Immunology 2022Quote: ... cells were harvested by scraping and immediately stained with live-dead 405nm stain Zombie Violet (BioLegend). After live-dead staining ...
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bioRxiv - Genomics 2020Quote: ... Cells were washed once in cold PBS and stained for 15 minutes with Zombi NIR (Biolegend) diluted 1:500 in PBS to label dead cells ...
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bioRxiv - Immunology 2021Quote: ... To discriminate B-cell subpopulations antibodies were used as follows: CD5- BV510 (clone 53-7.3, BioLegend), CD1d-FITC (clone 1B1 ...
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bioRxiv - Immunology 2021Quote: For intracellular IgE staining cells were also pre-blocked with anti-mouse IgE (RME-1, BioLegend) in 1:50 final dilution for 30 minutes ...
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bioRxiv - Physiology 2019Quote: ... and FITC conjugated antibodies against B220 to identify B cells (1:100, catalog number 103206, BioLegend). Secondary antibodies conjugated to AlexaFluor 405 (1:400 ...
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bioRxiv - Bioengineering 2019Quote: ... The single cell suspension was then washed and stained with Zombie Aqua (BioLegend, San Diego, USA), incubated with Fc Block (BioLegend ...
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bioRxiv - Cancer Biology 2019Quote: ... and 2×106 cells were incubated with mouse anti-HLA class I antibody (1:200, BioLegend) for 30min followed by secondary antibody incubation for 30min ...
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bioRxiv - Immunology 2020Quote: ... Identification of apoptotic cells by Annexin V staining was performed according to manufacturer instructions (Biolegend, 640934). Quantification of proliferation using EdU incorporation was accomplished by IP injection of 0.5mg EdU (Thermo Fisher Scientific E10187) ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated with CFSE (65-0850-85) alone or with αCD3 (clone HIT3a, #300302, Biolegend) and αCD28 (clone CD28.2 ...
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bioRxiv - Immunology 2021Quote: ... For the exclusion of dead cells Zombie Aqua fixable viability kit (BioLegend, San Diego, CA, USA) was adjoined and incubated for 30 min dRT ...
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bioRxiv - Immunology 2021Quote: ... 18 μL of PHA (1 μg/μL stock) and 2 μL of Cell activation cocktail (Biolegend) are added to the tube ...
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bioRxiv - Immunology 2021Quote: ... Quantification of total cell numbers by flow cytometry was done using fluorescent beads (Biolegend Precision beads). For intracellular staining of IFNγ and IL-2 in vitro ...
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bioRxiv - Immunology 2021Quote: ... the cells were lysed and fixed with 2 ml of 1x RBC Lysis/Fixation Solution (Biolegend) for 15 minutes at RT and washed with cold FACS-buffer (PBS with 1 % FCS) ...
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bioRxiv - Immunology 2021Quote: ... and for exclusion of dead cells Zombie Aqua fixable viability kit (BioLegend, San Diego, CA, USA) were used ...
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bioRxiv - Immunology 2021Quote: ... cells were washed in FACS buffer and stained using a Zombie Aqua Fixable Viability kit (Biolegend) and incubated for 20 minutes at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Cells were processed for flow cytometry and stained with BV421 anti-mouse CD45 (Biolegend, Cat# 103134), FITC anti-mouse CD71 (Biolegend ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were resuspended in FACS buffer with FC block (anti-CD16/CD32, 1:500, Biolegend 101330) and incubated for 5 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... all cell suspensions were Fc blocked with rat anti-mouse CD16/32 (clone 93, BioLegend, USA) and then stained with 10 μg/mL AlexaFluor647 Armenian hamster anti-mouse CD3ε (clone 145-2C11 ...
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bioRxiv - Neuroscience 2021Quote: ... and CD45-CD31+ cell fraction aliquots were incubated with anti-CD16/32 (BioLegend, 14-0161-82) to block Fc receptors ...
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bioRxiv - Cell Biology 2021Quote: ... CD4+CD25- T cells (Tconv) were purified by adding a biotinylated anti-CD25 antibody (PC61; Biolegend) to the CD4+ T cell negative isolation kit to deplete Treg cells ...
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bioRxiv - Microbiology 2020Quote: ... One million parasite of each sample/strain were washed twice in cell staining buffer (BioLegend, 420201) and resuspended in 100 μL of the LPG backbone specific CA7AE antibody at 0.125μL/100μL dilution in staining buffer ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then washed twice with 1x PBS + 2% FBS and fixed with Fluorofix Buffer (BioLegend) at RT for 30 minutes in the dark with shaking ...
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bioRxiv - Immunology 2021Quote: ... Live bone marrow cells were subject to FcR-receptor blocking (1:3; BioLegend; Cat. No. 101319) and viability staining (1:500 ...
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bioRxiv - Immunology 2021Quote: ... cell samples were stimulated in vitro with PMA/Ionomycin in the presence of Brefeldin A (BioLegend) and Monensin (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... Cells were then stained with the following antibodies: CD45 (Alexa Flour 700-Biolegend, Clone: 30-F11), CD11b (V450-BD Horizon ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated in blocking buffer (50 µL PBS/0.5% BSA, 1 µL FcR block [Biolegend]) for 20 minutes at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were washed and a cocktail of antibodies was added: CD11b (Biolegend, clone M1/70 #101207), Ly-6C (Biolegend ...
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bioRxiv - Cancer Biology 2020Quote: ... dissociated to single cells and stained with fluorescent conjugated antibodies for CD298 (Biolegend, cat. no. 341704) and MHC-I (eBioscience ...
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bioRxiv - Microbiology 2021Quote: ... cells were permeabilized using Intracellular Staining Permeabilization Wash Buffer (Perm Wash buffer; Biolegend; dilution 1:10), and stained intracellularly in 100 μl/well of anti-mouse IFN-γ (clone XMG1.2 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were resuspended in FACS buffer and stained with combination of APC-anti-Epcam (BioLegend, 118214) and PE/Cy7-anti-CD45 (Biolengend ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells for flow cytometry analysis were fixed for 20 min with a fixation buffer (BioLegend). For staining of intracellular antigens ...
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bioRxiv - Cell Biology 2021Quote: ... and an anti-FLAG AF549 conjugate (for cells expressing FLAG-tagged M; 1:1000, Biolegend, 637314) for 20 minutes at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... MCT-treated CD11b+ cells were isolated from tumors using MojoSort Mouse CD11b Selection Kit (BioLegend, 480109). For transfecting every million primary CD11b+ cells ...
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bioRxiv - Bioengineering 2022Quote: ... Activation of CD8 CAR-T cells was determined with CD25 staining using CD25-APC antibody (Biolegend). CAR expressions of ACE2 CAR and anti-Spike CAR and ACE2 expression of ACE2-293 cells were determined with SARS-CoV-2 S1 protein ...
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bioRxiv - Cell Biology 2022Quote: ... a suspension of mononuclear cells was stained with Alexa 700 anti-CD3 (1:100, BioLegend, 100215), PE-Cy7 anti-CD19 (1:100 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with intracellular monoclonal antibody-fluorochrome conjugates: IL-2-PE (BioLegend, cat.# 500307), TNF-α-FITC (BD ...
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bioRxiv - Physiology 2022Quote: RBCs present in a single-cell suspension were lysed using 1X RBC Lysis buffer (BioLegend, 420302) for 3 min at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: 1–2 × 106 NB4 treated cells were harvested and labelled with FITC-conjugated Annexin-V (Biolegend) for 15 min according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed and stained with BV711 conjugated anti-mouse CD8a (Biolegend, 1:200, cat# 563046), PE conjugated anti-mouse CD4 (Biolegend ...