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Citations for BioLegend :
5451 - 5500 of 6089 citations for Piggybac Transposable Element Derived 3 PGBD3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... The cell suspension was then either resuspended in the appropriate media for in vitro culture (discussed below) or incubated with 5µl/1ml/lung anti-mouse CD16/32 antibody (BioLegend, USA) for 30min at 4°C before further processing.
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bioRxiv - Immunology 2024Quote: ... and stained in media for 20 min at RT with Fixable Viability Dye 780 (eBioscience) and surface antibodies for CD19 (6D5, Biolegend), CD8α (53-6.7 ...
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bioRxiv - Immunology 2024Quote: ... the cells were washed again and stained a second time with a Pacific-Blue-conjugated anti-CD3 antibody (clone UCHT1, Biolegend) and a LIVE/DEAD-UV dye (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... The antibodies in the MDSC mastermix were used at individually titrated dilutions and included: CD33 clone P67.6 (Pe-Cy7, Biolegend #366617), CD14 clone MθP9 (APC-Cy7 ...
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bioRxiv - Immunology 2024Quote: ... Pelleted cells were suspended in FACS buffer and stained for 30 min at 4 °C with the desired antibody cocktails (BioLegend) of APC-Fire 750-anti CD11b (Cat ...
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bioRxiv - Immunology 2024Quote: ... cells were centrifuged and then resuspended in FACS buffer containing an antibody cocktail consistent of anti-CD11b AF488 (101217; Biolegend), anti-CD45 APC/Cy7 (157618 ...
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bioRxiv - Cancer Biology 2024Quote: ... Surface staining of the MDSC panel consisted of the following primary antibodies diluted in FACS buffer: anti-mouse CD45-Brilliant Violet 650 (1:200, 103151, Biolegend), anti-mouse F4/80-Brilliant Violet 785 (1:200 ...
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bioRxiv - Immunology 2024Quote: Surface staining of lung single cell suspensions was performed in PBS with 2% Newborn Calf Serum on ice for 30 minutes with the following antibodies: TruStain FcX PLUS (S17011E, BioLegend), APC-labeled Ly6G (1A8 ...
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bioRxiv - Physiology 2024Quote: ... The cell suspensions were treated with the fluorescent dye-conjugated antibodies and were labeled with Zombie Aqua Viability Dye (BioLegend). Then the cell suspensions were fixed with 4% paraformaldehyde ...
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bioRxiv - Pathology 2024Quote: PFA-fixed tissue sections were blocked with normal donkey serum (10% vol/vol) and incubated at 4 °C overnight with primary antibodies anti-F4/80 (123102, BioLegend), anti-Col1a1 (NB600-408 ...
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bioRxiv - Molecular Biology 2024Quote: ... TNFSF14 neutralization was achieved with a mouse anti-mouse LIGHT blocking antibody (clone 3D11, isotype control mouse IgG2b, clone 27-35, BioLegend), kindly provided by Prof ...
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bioRxiv - Molecular Biology 2024Quote: ... Primary antibodies were incubated overnight at 4°C in a humidified chamber: mouse anti-acetylated tubulin (1:400, BioLegend, 802001) and rabbit anti-GFP (1:500 ...
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bioRxiv - Immunology 2024Quote: ... the cells were harvested and labelled on the surface with the following monoclonal antibodies (Abs) from Biolegend (San Diego, USA): APC-conjugated anti-CD3 Abs (clone Mouse IgGak OKT3) ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were incubated with biotinylated anti-mouse antibodies (CD31, 1:150, BioLegend; CD45, 1:150, BioLegend; and Sca-1, 1:150, BioLegend) at 4°C for 40 min ...
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bioRxiv - Immunology 2024Quote: Naïve CD4+ T cells isolated from WT mice spleen and LN were treated with or without 10 µg/ml anti-β2 integrin non-function blocking antibody (BioLegend) for 1 hour and added onto 96 well plate coated with anti-CD3/CD28 along with either 2.5µg/ml of rLum or rBgn or rDcn or 1µg/ml of ICAM-1 for 30 minutes to check phospho-Akt-1 expression or 6 hours to check activation CD69 expression or 72 hours to check proliferation (CD4+ T cells were pre-labeled with CFSE for proliferation assay) ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T cells were then negatively selected from the mix by incubating on ice for 15 min with biotin conjugated antibodies: CD19 (115504, BioLegend) at 1:1000 ...
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bioRxiv - Immunology 2024Quote: ... Fcγ receptors were blocked prior to surface antigen staining by incubating the cells in flow cytometry staining buffer containing Human TruStain FcX antibody (BioLegend) for 15 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... Whole blood was immediately stained for flow cytometric analysis with combinations of the following human specific antibodies: CD4 (BV421, RPA-T4,Biolegend), CD4 (APC-Cy7 ...
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bioRxiv - Immunology 2024Quote: ... cells were stained for flow cytometry analysis with the following human specific antibodies: HLA-A,B,C(PE-Cyanine7, W6/32, Biolegend), CD4 (BV421 ...
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bioRxiv - Immunology 2024Quote: ... Isolation purity was verified by flow cytometry using antibodies against neutrophil surface markers anti-human CD11b (Alexa Fluor 594) (Biolegend) and anti-human CD15 (SSEA-1)(Alexa Fluor 488 ...
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bioRxiv - Immunology 2024Quote: ... Then cells were incubated in the dark for 20 minutes with 50µL of the antibody mixture: 1:50 each of anti-CD3-AF488 (BioLegend 100210), anti-CD4-BV510 (BioLegend 100559) ...
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bioRxiv - Bioengineering 2024Quote: ... and vendor information of antibodies used for the flow panel in this study was as follows: (i) Live/Dead (BV510, 1:100, 423111; BioLegend), (ii ...
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bioRxiv - Immunology 2024Quote: ... Surface CAR expression was stained for with an FMC63-biotin antibody (CytoArt) followed by a secondary staining with streptavidin-PE (BioLegend) in 1xPBS for 20 min at 4 °C protected from light ...
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bioRxiv - Immunology 2024Quote: ... they were randomly divided into two groups and one group was treated with IL-27-neutralizing antibody (Anti-mouse IL-27p28, Clone: MM27-7B1, Ultra LEAF purified; Biolegend) and the other with a non-relevant control antibody of the same isotype (Biolegend) ...
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bioRxiv - Immunology 2024Quote: ... After extensive washes by TBST, immunoblots were incubated with horseradish peroxidase-conjugated secondary antibodies (GE, IL) or peroxidase-conjugated avidin (BioLegend), and signals were detected using chemiluminescence.
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bioRxiv - Immunology 2024Quote: ... and customized TotalSeqTM-anti-mouse Hashtag Antibodies devoid of anti-CD45 (#155831, #155833, #155835, #155839, #155841, #155843) were purchased from Biolegend. Anti-Shp2 (clone 79 ...
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bioRxiv - Immunology 2024Quote: ... and fluorochrome-conjugated anti-CD86 and anti-CD32 antibodies in FACS buffer (1%BSA and 2 mM EDTA in PBS) supplemented with True Stain Monocyte Blocker solution (Biolegend) to prevent aspecific binding of fluorochrome-conjugated antibodies to myeloid cells ...
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bioRxiv - Immunology 2024Quote: Cells were collected and washed in FACS buffer (phosphate-buffered saline supplemented with 2% fetal bovine serum) and subsequently stained with the following antibodies purchased from BioLegend ...
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bioRxiv - Genomics 2024Quote: ... Lineage depletion was performed using magnetic columns (Miltenyi) with a custom biotinylated lineage antibody cocktail (CD8a, CD4, CD11b, Gr1, Ter119, B220; BioLegend) with streptavidin magnetic beads (Miltenyi ...
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bioRxiv - Immunology 2024Quote: ... Each cell sample in the same group was hash-tagged with a unique TotalSeq anti-mouse MHC-I antibody (Biolegend) on ice for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were washed with PBS containing 2 mM EDTA and stained with antibodies against CD59 (1:100, anti-human CD59 PE, Biolegend) and C3c (1:25 ...
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bioRxiv - Genomics 2024Quote: ... Primary antibody staining was carried out in the dark at 4 C for 30min with the following antibody dilutions in FACS buffer: 1:200 ICAM-1 (353102, Biolegend), 1:500 VCAM-1 (14-1069-82 ...
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bioRxiv - Biochemistry 2024Quote: ... for 15 min followed by the indicated antibodies cocktail for 60 min in the dark on ice: CD45 (FITC, clone S18009F, Biolegend), CD11b (BVC421 ...
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bioRxiv - Neuroscience 2024Quote: ... The remaining 80% cleared supernatant was transferred to a clean RNase-free tube and incubated with mouse anti-HA antibody (1:250; clone 16B12, Biolegend) on a rotating mixer for 4 h at 4°C while 300 uL protein G magnetic beads (Dynabeads ...
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bioRxiv - Immunology 2024Quote: ... ECS was performed during 15 min at room temperature with mix of anti-CD27 (O323)-FITC (or anti-IgG1 (MOPC-21)-FITC isotype control) and CD14 (QA18A22)-APC/Cy7 antibodies (Biolegend). Cells were fixed with fixation buffer (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Cells from individual skin tissue samples were simultaneously stained with corresponding mouse TotalSeq-B hashtag index antibodies (B0251-B0260; Biolegend), FACS antibody cocktail (CD45 ...
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bioRxiv - Bioengineering 2024Quote: ... Surface staining was carried out using a fluorochrome-labeled PE-conjugated anti-human CD11a (HI111) monoclonal antibody obtained from BioLegend.
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bioRxiv - Bioengineering 2024Quote: ... a staining solution was prepared by adding 5 µL of each fluorescent-conjugated antibody (anti-CD20-FITC, anti-CD45-APC, anti-CD56-APC-AF750, Zombie Violet; BioLegend) to FACS buffer ...
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bioRxiv - Bioengineering 2024Quote: ... for 5 mins on ice and stained with fluorochrome conjugated antibodies: CD19 (PerCP-Cy5.5, clone: 1D3, BioLegend, cat. Number 152406), CD95 (PE-Cy7 ...
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bioRxiv - Cancer Biology 2024Quote: ... ∼1×10.6 cells were stained with antibody cocktail at 4 °C for 20 min: Pacific Blue™ (PB) anti-mouse CD45.2 (Biolegend), APC anti-mouse B220 (Biolegend) ...
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bioRxiv - Cell Biology 2024Quote: ... the proteins from the native gel were transferred to the PVDF membrane and probed with an anti-His-tag antibody (BioLegend). For BN-PAGE ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed in flow media and incubated for 30 minutes on ice with fluorophore-conjugated anti-mouse antibodies (BioLegend) to detect CD45 (30-F11) ...
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bioRxiv - Cell Biology 2024Quote: ... hemolyzed lineage-positive and Sca-1-positive cells were labeled with biotin-conjugated antibodies and removed using MojoSort streptavidin nanobeads (BioLegend) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were transferred to a plate on ice and treated with 50 uL primary antibody against CD300a (Biolegend Cat#147901), diluted 1:50 in HBSS for 3 min ...
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bioRxiv - Cancer Biology 2024Quote: The samples from patients with BE were processed in the following way: Single-cell suspensions were stained with PE/Cy7-conjugated antibodies against CD45 (Biolegend) as a leukocyte marker and with propidium iodide (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then incubated on ice in the dark with APC anti-mouse Ly-6A/E (Sca-1) Antibody (clone D7, 108111, BioLegend) antibody for 45 min ...
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bioRxiv - Cancer Biology 2024Quote: ... for 10 minutes at 4°C and then surfaced-stained for 30 minutes at 4°C with the following antibodies obtained from BioLegend: anti-CD3-APC (Cat ...
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bioRxiv - Cell Biology 2024Quote: ... The PP and PC populations were identified using anti-E-cadherin-PE and anti-CD73-APC antibody staining (1:150; BioLegend). The PP- and PC-specific gates were set after spectral compensation using appropriate fluorescent minus (FMO ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.5 – 1 x 105 cells were washed with CSM and incubated with 100 µl antibody mix (1 µl APC anti-human CD19 (clone: HIB19, BioLegend); or 1 µl APC anti-human CD22 (clone ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed twice with CSM and incubated with 1 µl Pacific Blue anti-human CD19 antibody (clone: HIB19, BioLegend) in 100 µl CSM at RT for 10 min ...