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Citations for BioLegend :
5401 - 5450 of 6089 citations for Piggybac Transposable Element Derived 3 PGBD3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... For fluorescence staining the following primary/secondary antibodies and dyes diluted in PBS/10%NGS were used: α-EpCam/CD326 (clone G8.8, Biolegend), α-cleaved Caspase 3 (#9661 ...
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bioRxiv - Immunology 2023Quote: ... Cell staining followed a protocol developed in-house consisting of two separate panels of antibodies [Panel 1: CD14 (FITC) (#367116 BioLegend), CX3CR1 (PE ...
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bioRxiv - Immunology 2024Quote: ... cells from each well were washed thrice with PBS and stained with APC/Cyanine7 anti-human CD14 Antibody (BioLegend, #123317) to assess THP-1 cell phagocytosis of HER2-positive tumor cells ...
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bioRxiv - Immunology 2024Quote: ... Ltd. The fluorescent binding antibodies PE-CD11b (cat. no. 301306) and FITC-CD14 (cat. no. 301804) were purchased from BioLegend, Inc ...
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bioRxiv - Immunology 2024Quote: ... clone GK1.5-7; WEHI Antibody Facility); anti-IgD (1:200; clone 11-26c; eBioscience) and anti-GL7 (1:100; clone GL7; Biolegend).
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bioRxiv - Immunology 2024Quote: ... cells were incubated for 25 minutes at 4°C with the following antibodies: BV711 anti-mouse/human CD11b (Cat# 101242; BioLegend), PE anti-mouse CX3CR1 (Cat# FAB5825P ...
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bioRxiv - Immunology 2024Quote: ... were stained on ice for 25 minutes with the following panel of lineage-specific antibodies: Biotin-labeled TER-119 (TER-119, BioLegend), Biotin-labeled GR-1 (RB6-8C5 ...
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bioRxiv - Immunology 2024Quote: ... samples were incubated overnight at 4°C with combinations of the following antibodies: PE-Cy7-labeled CD63 (NVG-2, BioLegend), AF532-labeled iNOS (CXNFT ...
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bioRxiv - Immunology 2024Quote: ... Surface stains were incubated for 30-60 minutes at room temperature using a selection of the following antibodies: TruStain FcX PLUS (S17011E, BioLegend), BUV496-labeled CD45 (30-F11 ...
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bioRxiv - Bioengineering 2024Quote: ... for 5 min on ice and then stained with fluorochrome conjugated antibodies: CD19 (PerCP-Cy5.5, clone: 1D3, BioLegend, cat: 152406) CD95 (PE-Cy7 ...
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bioRxiv - Immunology 2024Quote: ... It exhibits monocyte morphology and was originally derived from malignant cells obtained from the pleural effusion from a patient with histiocytic lymphoma.77 LFA-1 (CD11a/CD18) was assessed with a FITC anti-human antibody (clone m24, Biolegend), and MAC-1 (CD11b/CD18 ...
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bioRxiv - Immunology 2024Quote: ... low endotoxin (Super affinity) (Acro) followed by secondary staining with APC conjugated anti-human IgG Fc Antibody (Biolegend, cat# 410712) and GFP/RFP expressions ...
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bioRxiv - Immunology 2024Quote: ... in the dark for 15 min at room temperature and labelled with anti-GFP and anti-RFP primary antibodies in Cyto-Fast Perm/Wash solution 1X (#750000135, BioLegend) for 30 min on ice following washing with Cyto-Fast Perm/Wash solution 1X and labelling with AF488-anti-mouse or AF647-anti-rabbit antibodies in Cyto-Fast Perm/Wash solution 1C for 30 min on ice in the dark ...
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bioRxiv - Microbiology 2024Quote: ... the cells were resuspended with the CD3 T-cell antibody conjugated with Brilliant Violet 605 (Clone: 17A2; Cat. No. 100237) purchased from BioLegend at the desirable concentration (1:100 ...
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bioRxiv - Cancer Biology 2024Quote: Single cell suspensions from tumors were washed with HBSS containing 2% FBS and 1 mM EDTA and stained with the following antibody cocktails for detecting lymphoid populations: anti-CD45-PerCP/Cy5.5 (BioLegend 103132), anti-NK1.1-FITC (BioLegend 108706) ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated for 30 min at 4°C with 1 µL of PE anti-human FasL antibody (clone NOK-1) from BioLegend. Nonspecific background signals were measured by incubating cells with the appropriate isotype-matched antibody ...
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bioRxiv - Immunology 2024Quote: ... cells were centrifuged and then resuspended in FACS buffer containing an antibody cocktail consistent of anti-CD11b AF488 (101217; Biolegend), anti-CD45 APC/Cy7 (157618 ...
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bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the Jurkat staining panel: anti-CD4 FITC (BioLegend catalog #317408, clone OKT4), anti-CD25 PerCP/Cyanine (Cy ...
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bioRxiv - Bioengineering 2024Quote: ... 100 μL of staining solution containing APC SIINFEKL tetramer (1:600 dilution) and fluorescein isothiocyanate (FITC) CD8 antibody (1:100 dilution, Biolegend) was added to each tube in the ice bath and incubated for 30 minutes in the dark ...
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bioRxiv - Cell Biology 2024Quote: ... was then carried out following the Chromium Next GEM Single Cell 3’ protocol for v3.1 reagents from 10X Genomics (CG000206 Rev D) and TotalSeq™-A Antibodies and Cell Hashing protocol from Biolegend (available online at https://www.biolegend.com/en-gb/protocols/totalseq-a-antibodies-and-cell-hashing-with-10x-single-cell-3-reagent-kit-v3-3-1-protocol) ...
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bioRxiv - Immunology 2024Quote: ... The following anti-mouse antibodies were used for flow cytometry: anti-CD45 BV711 (Biolegend Clone, clone 30-F11, lot B366812); anti-CD3 PE (Biolegend Clone 17A2 ...
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bioRxiv - Physiology 2024Quote: ... The cell suspensions were treated with the fluorescent dye-conjugated antibodies and were labeled with Zombie Aqua Viability Dye (BioLegend). Then the cell suspensions were fixed with 4% paraformaldehyde ...
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bioRxiv - Molecular Biology 2024Quote: ... TNFSF14 neutralization was achieved with a mouse anti-mouse LIGHT blocking antibody (clone 3D11, isotype control mouse IgG2b, clone 27-35, BioLegend), kindly provided by Prof ...
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bioRxiv - Immunology 2024Quote: ... splenocyte and microglial cell Fc-receptors were blocked using a purified anti-CD16/CD32 antibody (1:200, clone 93, BioLegend) for 20 minutes at 4°C ...
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bioRxiv - Immunology 2024Quote: White blood cell and microglial cell Fc-receptors were blocked using a purified anti-CD16/CD32 antibody (1:200, clone 93, BioLegend) for 20 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained with the following anti-mouse antibodies: Brilliant Violet 785 CD45 (30-F11, catalog no. 103149, Biolegend), PerCP/Cyanine 5.5 CD5 (53-7.3 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were resuspended in 100 µL of FACS Buffer and stained for 15 minutes at room temperature with an antibody cocktail containing anti-CD71 (1:50; PerCP/Cyanine 5.5; BioLegend, 334113), anti-CD235a (1:500 ...
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bioRxiv - Immunology 2024Quote: ... Membranes were washed in TBS-T and incubated overnight at 4 ◦C with monoclonal pNF-κB p65 (Ser 529)-specific antibody (1:500 dilution, clone 817403, Biolegend), monoclonal NF-κB p65-specific antibody (1:2500 dilution ...
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bioRxiv - Immunology 2024Quote: ... a combined antibody solution was prepared in FACS buffer with fluorophore-conjugated antibodies: anti-mouse Alexa fluor 700 CD45 (Cat. No. 103128, BioLegend), anti-mouse PerCP/Cyanine 5.5 CD11b (Cat ...
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bioRxiv - Immunology 2024Quote: ... Each cell sample in the same group was hash-tagged with a unique TotalSeq anti-mouse MHC-I antibody (Biolegend) on ice for 30 min ...
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bioRxiv - Immunology 2024Quote: Immune cells isolated above were stained with the following antibodies (Live/Dead Fixable NIR, Invitrogen, #L34975, 1:500; CD45, Pacific Blue, Biolegend, #103126 ...
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bioRxiv - Immunology 2024Quote: ... ECS was performed during 15 min at room temperature with mix of anti-CD27 (O323)-FITC (or anti-IgG1 (MOPC-21)-FITC isotype control) and CD14 (QA18A22)-APC/Cy7 antibodies (Biolegend). Cells were fixed with fixation buffer (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Surface CAR expression was stained for with an FMC63-biotin antibody (CytoArt) followed by a secondary staining with streptavidin-PE (BioLegend) in 1xPBS for 20 min at 4 °C protected from light ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T cells were then negatively selected from the mix by incubating on ice for 15 min with biotin conjugated antibodies: CD19 (115504, BioLegend) at 1:1000 ...
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bioRxiv - Immunology 2024Quote: Naïve CD4+ T cells isolated from WT mice spleen and LN were treated with or without 10 µg/ml anti-β2 integrin non-function blocking antibody (BioLegend) for 1 hour and added onto 96 well plate coated with anti-CD3/CD28 along with either 2.5µg/ml of rLum or rBgn or rDcn or 1µg/ml of ICAM-1 for 30 minutes to check phospho-Akt-1 expression or 6 hours to check activation CD69 expression or 72 hours to check proliferation (CD4+ T cells were pre-labeled with CFSE for proliferation assay) ...
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bioRxiv - Immunology 2024Quote: ... Whole blood was immediately stained for flow cytometric analysis with combinations of the following human specific antibodies: CD4 (BV421, RPA-T4,Biolegend), CD4 (APC-Cy7 ...
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bioRxiv - Immunology 2024Quote: ... cells were stained for flow cytometry analysis with the following human specific antibodies: HLA-A,B,C(PE-Cyanine7, W6/32, Biolegend), CD4 (BV421 ...
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bioRxiv - Immunology 2024Quote: ... a combined antibody solution was prepared in FACS buffer with fluorophore-conjugated antibodies: anti-mouse Alexa fluor 700 CD45 (Cat. No. 103128, BioLegend), anti-mouse PerCP/Cyanine 5.5 CD11b (Cat ...
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bioRxiv - Immunology 2024Quote: ... The cell suspension was then either resuspended in the appropriate media for in vitro culture (discussed below) or incubated with 5µl/1ml/lung anti-mouse CD16/32 antibody (BioLegend, USA) for 30min at 4°C before further processing.
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bioRxiv - Immunology 2024Quote: ... and stained in media for 20 min at RT with Fixable Viability Dye 780 (eBioscience) and surface antibodies for CD19 (6D5, Biolegend), CD8α (53-6.7 ...
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bioRxiv - Immunology 2024Quote: ... and customized TotalSeqTM-anti-mouse Hashtag Antibodies devoid of anti-CD45 (#155831, #155833, #155835, #155839, #155841, #155843) were purchased from Biolegend. Anti-Shp2 (clone 79 ...
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bioRxiv - Immunology 2024Quote: ... After extensive washes by TBST, immunoblots were incubated with horseradish peroxidase-conjugated secondary antibodies (GE, IL) or peroxidase-conjugated avidin (BioLegend), and signals were detected using chemiluminescence.
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bioRxiv - Immunology 2024Quote: ... they were randomly divided into two groups and one group was treated with IL-27-neutralizing antibody (Anti-mouse IL-27p28, Clone: MM27-7B1, Ultra LEAF purified; Biolegend) and the other with a non-relevant control antibody of the same isotype (Biolegend) ...
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bioRxiv - Immunology 2024Quote: ... Then cells were incubated in the dark for 20 minutes with 50µL of the antibody mixture: 1:50 each of anti-CD3-AF488 (BioLegend 100210), anti-CD4-BV510 (BioLegend 100559) ...
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bioRxiv - Immunology 2024Quote: ... Fcγ receptors were blocked prior to surface antigen staining by incubating the cells in flow cytometry staining buffer containing Human TruStain FcX antibody (BioLegend) for 15 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T-cells from the mouse ear were sorted with a FACSAria sorter from samples treated with BHI-tween or HSV-1 and labeled with TotalSeqC hashtags antibodies (Biolegend). All samples were pooled together ...
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bioRxiv - Immunology 2024Quote: ... samples were stained with the antibody cocktail for ImageStream analysis (UCHT1-PE, Biolegend; CD19- AF594, Biolegend; CD14-BV421, Biolegend; CD8-AF700, Biolegend; CD4-APC/Fire750 ...
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Kir6.1, a component of an ATP-sensitive potassium channel, regulates natural killer cell developmentbioRxiv - Immunology 2024Quote: ... either whole spleen or isolated NK cells were incubated in PEB buffer (PBS+0.5% BSA+2mM EDTA) with TruStain FcX™ (anti-mouse CD16/32) antibody (Biolegend) on ice for 5 minutes ...
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bioRxiv - Immunology 2024Quote: ... Fluorescently labeled anti-mouse antibodies against CD45 (104) and H-2Kb/H-2Db (28-8-6) were obtained from BioLegend.
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bioRxiv - Immunology 2024Quote: ... and fluorochrome-conjugated anti-CD86 and anti-CD32 antibodies in FACS buffer (1%BSA and 2 mM EDTA in PBS) supplemented with True Stain Monocyte Blocker solution (Biolegend) to prevent aspecific binding of fluorochrome-conjugated antibodies to myeloid cells ...