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Citations for BioLegend :
5351 - 5400 of 6089 citations for Piggybac Transposable Element Derived 3 PGBD3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Harvested lungs and spleen immune cells were incubated with recommended dilutions of antibodies conjugated to fluorophores: CD3-BV510 (BioLegend, #100234), CD4-BV785 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Phospho-protein stain was performed using an optimized protocol as follows: Following stain with antibodies against surface markers CD4 and IL-23R (12B2B64, Biolegend), the cells were fixed by 2% paraformaldehyde for 10 min at room temperature and washed once with cold PBS ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 106 cells were incubated with PE anti-CD31 ([WM59] 303105), PE anti-CD45 ([2D1] 368510) and PE anti-CD326 (EpCAM, [9C4] 324206) antibodies (1/100, BioLegend) and human TruStain FcXTM (1/200 ...
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bioRxiv - Cancer Biology 2023Quote: ... EMT phenotype of in vivo isolated tumor cells was analyzed using the same antibody panel implemented with anti-mouse CD45 (30-F11; Biolegend), CD90.1 (OX-7 ...
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bioRxiv - Genomics 2022Quote: ... The custom oligos were synthesized by Integrated DNA Technologies and conjugated to anti-Nuclear Pore Complex Proteins antibody (Clone Mab414, BioLegend) by BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Cells were collected and stained with fluorescently labeled antibodies against CD45 PE-Cy 7 (clone: 30-F11, cat # 103114: Biolegend), TCRβ APC (clone ...
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bioRxiv - Immunology 2023Quote: ... cells were washed three times with FACS buffer (PBS+0.5% BSA+2% FBS+0.1% sodium azide) and stained with the following antibodies for 30 minutes: anti-CD4 PerCP/Cy5.5 (BioLegend Cat: 100540), anti-CD8a Brilliant Violet 510 (BioLegend Cat ...
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bioRxiv - Cell Biology 2023Quote: ... Long bones and iliac crests were isolated and bone marrow was obtained by centrifugation at 2500g for 40s as previously described.41 Hematopoietic stem and progenitor cells (HSPCs) were separated by lineage depletion using an antibody mixture (Lineage depletion panel, 133307, Biolegend) and magnetic beads (CD4 untouched ...
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bioRxiv - Genomics 2023Quote: ... The cells were then incubated on ice for another 30 min after adding 0.5 μl of biotin anti-human CD15 antibody (BioLegend, 301913). After immunostaining ...
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bioRxiv - Immunology 2023Quote: ... Cells were centrifuged at 400 g for 8 minutes and stained with lanthanide-labeled antibodies against immunophenotypic markers in CyTOF buffer containing Fc receptor blocker (BioLegend) for 30 minutes at room temperature (RT) ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were stained for 30 min at 4°C with surface antibodies: anti-mouse CD3 (Biolegend, APC Fire 810), CD4 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... and goat anti-rabbit Alexa Fluor Plus 647-coupled secondary antibody (1:500) in Intracellular Staining Permeabilization Wash Buffer (Biolegend). The fraction of cells positive for cleaved caspase-3 was measured with a BD LSRFortessa SORP flow cytometer ...
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bioRxiv - Immunology 2023Quote: ... 0.5-1×106 bone marrow cells were stained with the following surface antibodies (2 panels) against: CD14 (Biolegend, M5E2, AF700), HLA-DR (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... and cells without either EpCam or CD45 markers were sorted using antibodies against EpCam (clone G8.8, Biolegend, San Diego, CA) or CD45 (clone 30-F11 ...
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bioRxiv - Immunology 2023Quote: ... Serum samples were assessed for titers of different antibody isotypes using LegendPlex Mouse Immunoglobulin Isotyping Panel and IgE ELISA (BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were incubated one hour in 5% milk before adding the primary antibody (anti-HA 3F10, Roche 27573500, 1:2500, anti-Ubiquitin P4D1, Biolegend, 1:10,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... for dead cell exclusion followed by Fc-receptor blocking with purified mouse CD16/32 antibody (BioLegend, catalog #101301, clone 93). After cell surface marker staining with fluorescent-conjugated antibodies ...
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bioRxiv - Bioengineering 2023Quote: ... The expression of Mo surface markers was measured by flow cytometric analysis using fluorescently labeled antibodies against CD11b (BioLegend #101207), CD45R (BioLegend #103211) ...
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bioRxiv - Cell Biology 2023Quote: ... Satellite cells were FACS-purified using either Pax7-nGFP reporter or the following combinations of cell surface markers: APC anti-mouse Ly-6A/E (Sca-1) Antibody (108111, BioLegend), mouse Integrin alpha 7 Alexa Fluor 750-conjugated Antibody (FAB3518S ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies used to distinguish the different cell populations included Brilliant violet 421 anti-human CD19 (Biolegend, San Diego, CA, USA), PE anti-human CD45 (Biolegend) ...
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bioRxiv - Cancer Biology 2023Quote: ... 90% of the thawed cells (>200 x 106) were stained with biotinylated antibodies against CD45 (1:50, clone HI30; Biolegend) and CD235a (1:50 ...
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bioRxiv - Immunology 2023Quote: ... Lung resident cells were distinguished from those in the vasculature by exclusion from staining with fluorescently labeled anti-CD45 antibody (PerCP, clone 30F-11, BioLegend) that was injected retro-orbitally into each mouse approximately 3 minutes prior to euthanasia65 ...
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bioRxiv - Immunology 2023Quote: ... followed by staining with a premixed cocktail of the indicated tetramers at a final concentration of 10 nM for 1 hour at RT in the presence of anti-mouse CD16/32 antibody (clone 93, BioLegend). SLO preparations were magnetically enriched for tetramer-specific cells as previously described64 while lung samples were magnetically enriched for CD90.2 expression (Miltenyi) ...
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bioRxiv - Immunology 2022Quote: ... For fluorescence staining the following primary/secondary antibodies and dyes diluted in PBS/10%NGS were used: α-EpCam/CD326 (clone G8.8, Biolegend), α-cleaved Caspase 3 (#9661 ...
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bioRxiv - Immunology 2023Quote: ... Cell staining followed a protocol developed in-house consisting of two separate panels of antibodies [Panel 1: CD14 (FITC) (#367116 BioLegend), CX3CR1 (PE ...
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bioRxiv - Immunology 2023Quote: ... Purified proteins were then verified by SDS-PAGE as well as by capturing proteins on streptavidin beads and immunocytometry with anti-CD40L and anti-His tag antibodies (Biolegend), and compared to commercial soluble CD40L (ACROBiosystems).
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bioRxiv - Bioengineering 2023Quote: ... CD3/CD28 Beads were magnetically removed and transduction efficacy was analyzed by staining with fluorophore-conjugated antibodies (all from Biolegend) and 7-AAD staining solution (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were labeled with the following antibodies:(CEACAM6-FITC at 10 μg/mL, Sino Biological 10823-R408R; H2Kb-Pac Blue at 0.5 mg/mL, BioLegend 116514 ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated for 30 minutes on ice with the following antibody: anti-CD44 APC/Fire 750 (1:100, Biolegend, 103061), anti-CD24 PE (1:20 ...
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bioRxiv - Molecular Biology 2023Quote: ... The percentage of purified CD34+ cell was analysed by flow cytometry staining with anti-human CD34 PE antibody (BioLegend, UK). The cells were cultured in StemSpan SFEM II medium (StemCell Technologies ...
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bioRxiv - Molecular Biology 2023Quote: Cells were incubated for 30 minutes at room temperature in 0.5% BSA PBS with 1:50 CD81-FITC antibody (Biolegend, 349504) or mouse IgG1 FITC isotype control antibody (Biolegend ...
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bioRxiv - Physiology 2023Quote: ... single cell suspension from mouse esophagi was incubated at 37°C for 30 minutes with Integrin β1 antibody (PE anti-mouse/rat CD29; 102207; Biolegend) at 1:200 dilution ...
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bioRxiv - Microbiology 2023Quote: ... Antibodies used were Fluoroscein isothiocyanate (FITC)-conjugated anti-mouse CD4 and CD21/CD35 (CR2/CR1: Biolegend, San Diego, CA, USA) and Phycoerythrin (PE)-conjugated anti-mouse CD3 (17A2 ...
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bioRxiv - Immunology 2023Quote: ... from uninfected or MCMV-infected WT C57BL/6 mice were sorted as described and stained with barcoded antibodies (Total-Seq B, BioLegend). After hash-staining ...
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bioRxiv - Immunology 2023Quote: Peyer’s patch leukocytes from 10 WT B6 mice de novo colonized for 21 days with SFB were separately stained with oligo-hashtagged antibodies (TotalSeqC 1 – 10, Biolegend), prior to pooling ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies and dilutions used for western blot were: Mouse Anti-Aβ (6E10 clone, 1:1,000, Biolegend, Cat# 803001, RRID:AB_2564653), goat anti-Mouse DKK-3 (1:1,000 ...
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bioRxiv - Neuroscience 2023Quote: ... the following antibodies were added in a 10 µl master mix: CD11b-FITC (M1/70, 1:400, 1:400, Biolegend), CD45-APC/Cy7 (30F11 ...
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bioRxiv - Pathology 2023Quote: ... Ldlr−/− mice fed a chow diet or HC diet for 4 days were anesthetized using Isoflurane and injected intravenously with 5 μg anti-mouse TIM4-APC antibody (RMT4-54, BioLegend). They were then euthanized 5 minutes after injection by cervical dislocation and perfused through the portal vein with PBS and then with 4% ice-cold PFA ...
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bioRxiv - Genetics 2023Quote: ... and 72 h by immunofluorescent staining with a PE-conjugated anti-human GPA primary antibody (BioLegend, San Diego, CA, USA) using flow cytometric analysis.
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bioRxiv - Immunology 2023Quote: ... thawed PBMCs were FACS-sorted to isolate CD8+ CD4- CD56- cells (using BV785-conjugated anti-CD8 antibody, clone SK1 (Biolegend); PerCP-Cy5.5-conjugated anti-CD4 antibody ...
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bioRxiv - Immunology 2023Quote: ... along with co-stimulatory antibodies (CD28 and CD49d at 1 μL/mL) and Brefeldin-A (1 μL/mL, BioLegend, USA). LIVE/DEAD and surface staining was performed as described above ...
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bioRxiv - Immunology 2023Quote: ... For comparison of resident vs non-resident populations tonsil samples were divided into CD69+ and CD69- subsamples using biotinylated anti-human CD69 antibody (FN40, Biolegend), and separation was performed using anti-Biotin Microbeads (Milenyi ...
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bioRxiv - Immunology 2023Quote: ... CD4+CD62LhighCD44low/- T cells were purified from the spleen of wild-type BALB/c mice post staining with antibodies to mouse CD3 (17A2, Biolegend), CD4 (RM4-5 ...
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bioRxiv - Cancer Biology 2023Quote: ... non-natural killer cells were depleted by incubating the patient’s whole PBMCs sample with the biotin antibody cocktail (BioLegend 480053) followed by incubation with magnetic Streptavidin Nanobeads ...
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bioRxiv - Cancer Biology 2023Quote: ... mice were injected intraperitoneally with three hundred micrograms of Ultra-LEAF purified anti-Asialo GM1 rabbit polyclonal antibody (clone Poly21460, BioLegend) every four days for the duration of the experiment ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed once with FACS buffer and pre-incubated with 5 μg/mL TruStain FcX anti-mouse CD16/32 antibody (clone 93, BioLegend) in 100 μL FACS buffer for 5 minutes on ice before immunostaining ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with a combination of fluorochrome-conjugated antibodies to the following surface markers (from Biolegend unless otherwise indicated): CD3ε (17A2 ...
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bioRxiv - Biochemistry 2023Quote: ... Cell pellets were collected and resuspend by 500 μL (for 80 mL cord blood) Dilution Buffer and then incubated by 10 μL CD34-APC antibody (343510, BioLegend) on ice for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies, rabbit anti-phospho-cofilin (CST, #3311, 1:100) and mouse anti-SMI-32 (BioLegend, # SMI-32R, 1:250), were applied in blocking buffer at 4°C overnight ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected at d5 of the culture for the analysis of T cell cytokine production using a combination of commercially available antibodies (Biolegend). Briefly ...