Labshake search
Citations for BioLegend :
5601 - 5650 of 6089 citations for Piggybac Transposable Element Derived 3 PGBD3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... Multiplexing was performed by washing and hashing nuclei from each sample with one of six unique TotalSeq™-A anti-nuclear pore complex antibodies (BioLegend). Two multiplexed sequencing batches were then sequenced by the Oxford Genomics Centre (Wellcome Centre for Human Genetics ...
-
bioRxiv - Cancer Biology 2022Quote: ... The washed samples were stained for 20 min on ice with Brilliant Violet 510™ anti-mouse CD45 Antibody (1:200, Clone: 30-F11, BioLegend), PE/Cyanine7 anti-mouse/human CD11b Antibody (1:200 ...
-
bioRxiv - Bioengineering 2022Quote: ... fluorescence minus one (FMO) + isotype control antibody staining was performed using the following isotypes: PE/Cy7 Mouse IgG2a κ (cat # 400232, BioLegend), BV-421 Mouse IgG1 κ (cat # 400158 ...
-
bioRxiv - Cell Biology 2022Quote: Cell suspensions from thymus were incubated with the biotin-conjugated anti-lineage marker antibodies described above together with CD4-APC (Biolegend 100411), CD8b-APC/Cy7 (Biolegend 126620) ...
-
bioRxiv - Cell Biology 2022Quote: ... The cells were then stained with a PE-labeled anti-huCD4 antibody (SK3 clone, Cat#344606, BioLegend, San Diego, CA, USA) and incubated in the dark for 45 min at 4°C ...
-
bioRxiv - Cell Biology 2022Quote: ... EGFR knockout cells were also validated by testing for loss of tyrosine phosphorylation in response to a one-minute treatment with 50 ng/mL mouse EGF by immunoblotting with mouse monoclonal antibody (PY20, BioLegend, 309301).
-
bioRxiv - Immunology 2022Quote: ... for 15 minutes and subsequently stained with a combination of fluorophore-conjugated primary antibodies against mouse CD45 (BioLegend clone 30-F11), CD11b (BioLegend clone M1/70) ...
-
bioRxiv - Immunology 2022Quote: ... Cells were washed with PBS supplemented with 2% FBS (PBS+) and then stained with 50µl of an antibody cocktail containing 1:50 diluted anti-human CD45-AF700 (BioLegend, clone HI30), 1:50 diluted anti-human CD3-BV605 (BioLegend ...
-
bioRxiv - Immunology 2022Quote: ... Primary antibodies, anti-PDGFRa (AB Online, # ABIN726620), anti-Kitl (R&D, #AB- 455-NA) and anti-c-Kit (Biolegend, clone 2B8) were then stained at a 1:200 dilution for 72hrs ...
-
bioRxiv - Genomics 2022Quote: ... Cell pellets were resuspended in FACS buffer (PBS with 1% FBS and 1% penicillin-streptomycin) then stained with the following antibodies: PE anti-CD45 (BioLegend #103105); APC anti-CD31 (Invitrogen #17-0311-80) ...
-
bioRxiv - Immunology 2022Quote: ... Blocking and antibody solutions were further supplemented with 1:150 Human TruStain FcX™ Fc Receptor Blocking Solution (BioLegend, London, UK), to reduce non-specific binding ...
-
bioRxiv - Immunology 2022Quote: ... and incubated with the following antibodies in cRPMI at 1:200 for 30 minutes: CD4-BV650 (#100469; Biolegend, San Diego, USA), CD8-BV605 (#100744 ...
-
bioRxiv - Immunology 2022Quote: ... Immunostaining was performed by incubating the sections overnight at 4°C with the SMI-32 primary antibody (Biolegend: Suppl. Table 1) in incubation buffer ...
-
bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated in primary antibodies diluted in 5% skim milk in TBST: mouse anti-brevican (1:1000, BioLegend, cat# 820101), sheep anti-neurocan (1:1000 ...
-
bioRxiv - Genomics 2022Quote: ... and stained at room temperature for 15-30 min with APC-conjugated antibodies targeting CD55 (clone JS11, BioLegend 311311, RRID AB_2075857), CD81 (clone 5A6 ...
-
bioRxiv - Neuroscience 2022Quote: ... Then the live microglial cells were incubated with PBS supplemented with 5% BSA + HLA-DR mouse monoclonal antibodies pre-conjugated with FITC (1:100, BioLegend, Inc.) + Hoechst 33342 (1:5000 ...
-
bioRxiv - Synthetic Biology 2022Quote: ... The membrane was incubated with 5% nonfat milk in TBST for 1 hour followed by the addition of primary antibodies (BioLegend, 652505). After 1 hour of the primary antibody incubation ...
-
bioRxiv - Cancer Biology 2022Quote: ... Tissue sections were stained with monoclonal mouse anti-βIII-tubulin primary antibody (Biolegend, Cat. 801202; 1:100 overnight at 4 °C) and goat anti-mouse AF647 (abcam ...
-
bioRxiv - Immunology 2024Quote: Cells were stained with 20 µL of surface antibody cocktail with Zombie NIR fixable viability kit (1:400-1000, Biolegend, 423105) in PBS for 20 minutes on ice ...
-
bioRxiv - Cell Biology 2024Quote: ... all input solutions were supplemented with 1 μg/mL of PE-conjugated rat anti-mouse TNFα antibody (MP6-XT22, BioLegend, UK).
-
bioRxiv - Cell Biology 2024Quote: ... The cytokine capture bead for the infectious disease workflow was generated by conjugating an anti-mouse TNFα antibody (#6B8, BioLegend, UK) to 6.7 μm carboxyl polystyrene beads according to Spherotech guidelines.
-
bioRxiv - Immunology 2024Quote: ... Some mice were intraperitoneally (i.p.) injected with Ultra-LEAF Purified anti-mouse NK-1.1 Antibody (clone#PK136; 200 µg/mouse, Biolegend, San Diego, CA) every other day ...
-
bioRxiv - Immunology 2023Quote: ... catalog #13-0870-T500) and surface stained with the following antibodies in an appropriate combination of fluorochromes: CD45.2 (BioLegend, clone 104), Epcam (BioLegend ...
-
bioRxiv - Immunology 2024Quote: Naïve or PR8 infected B6 mice were administered daily 20µg anti-CCR2 depleting antibodies (MC-21 generously provided by Prof. Matthias Mack, Universität Regensburg) or rat IgG2b,κ isotype control (Biolegend, #400644) intraperitoneally (i.p.) ...
-
bioRxiv - Immunology 2024Quote: ... the enriched epithelial cell suspension was stained for flow cytometry cell sorting with the following antibodies and dyes: anti-EpCAM-APC-Cy7 clone G8.8 (BioLegend, cat. 118218), anti-CD45-APC clone 30-F11 (BD Biosciences ...
-
bioRxiv - Developmental Biology 2024Quote: ... then incubated for 1 hr at 4°C with the following antibodies: CD26 (DPP4)-fluorescein isothiocyanate (FITC) (Biolegend, 137806; 1:200), anti-mouse ICAM1-phycoerythrin (PE)/Cy7 (Biolegend ...
-
bioRxiv - Developmental Biology 2024Quote: ... blocked in 3% BSA/ 5% normal goat serum in PBS for 1h and incubated overnight at 4°C with primary antibodies (β-III tubulin/anti-tuj-1, 1/1000, Biolegend n°801202 ...
-
bioRxiv - Immunology 2024Quote: The following antibodies were used: unconjugated anti-CD16/CD32 (1 μg per 1×106 cells; clone 93; BioLegend Cat. No. 101320), H2-Dd-FITC (1:200 ...
-
bioRxiv - Immunology 2024Quote: ... Cells were next stained for 30 mins on ice with a 1:300 dilution of antibodies raised against mouse CD206 (Biolegend,141710), CD301(Biorad ...
-
bioRxiv - Immunology 2024Quote: ... followed by 5 µL/well of the antibody cocktail for extracellular staining: anti-CD3-BV421 (clone 17A2) (BioLegend, SanDiego, CA, USA) (1:200) ...
-
bioRxiv - Immunology 2023Quote: ... HTO stained PBMCs from all 10 donors were then pooled and stained with oligo-tagged TotalSeq C antibody for cell surface proteins for T cell memory markers (CD45RA, CCR7, CD95 and IL7Rα) (0.5 µg/condition; Biolegend, Table S2B) for 30 minutes on ice ...
-
bioRxiv - Bioengineering 2024Quote: ... were coated with 100 µL of 4 µg/mL polyclonal anti-lysostaphin antibody (AICBIOTECH-PAb102) in 1×ELISA coating buffer (BIOLEGEND-421701). Wells were washed with 0.05% Tween 20 (SIGMA-P9416-50ML ...
-
bioRxiv - Cell Biology 2024Quote: ... The cell suspensions were then co-incubated with fixable viability dye (eFluor™ 780, 65-0865-14, eBioscience) and antibodies against surface markers CD45(PE/Cy7, 147703, BioLegend), Cd3e(APC ...
-
bioRxiv - Biophysics 2023Quote: ... then cells were stained overnight with DAPI and an Alexa Fluor 647-conjugated anti-cytochrome c antibody (Biolegend, clone 6H2.B4). Stained cells were analyzed using an Attune NxT flow cytometer ...
-
bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-mouse CD45.1 (Biolegend, cat.: 110735), anti-mouse TER-119 (eBiosciences ...
-
bioRxiv - Microbiology 2023Quote: ... The membrane was incubated in 1% milk-PBS-T containing rabbit anti-LpxC antibody (a generous gift from the Doerrler lab) or mouse anti-RpoA (anti-E.coli RNA polymerase alpha from Biolegend, cat# 663104) diluted 1:10,000 ...
-
bioRxiv - Molecular Biology 2023Quote: ... and APC/Fire 750 conjugated anti E-Cadherin (dilution: 1:100; clone DECMA-1. All antibodies purchase from BioLegend (CA, USA). Samples were washed in 1mL 2% FBS and PBS and centrifuged at 2000rpm for 5mins ...
-
bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 500xg for 5 minutes and then resuspended in 50uL of PBS+ 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302). Cells were labelled with antibodies as described below ...
-
bioRxiv - Immunology 2023Quote: ... Cells were stained with Zombie-violet or Zombie-NIR in PBS at 1:2000 dilution for 5 minutes at room temperature prior to resuspending in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302) for blocking ...
-
bioRxiv - Cancer Biology 2023Quote: ... murine thymocytes were first stained using CD4 and CD8 antibodies and then fixed and permeabilized using Fixation Buffer (Biolegend cat 420801) and Phosflow Perm Buffer III (cat 558050 ...
-
bioRxiv - Synthetic Biology 2022Quote: ... The membrane was incubated with 5% nonfat milk in TBST for 1 hour followed by the addition of primary antibodies (BioLegend, 652505). After 1 hour of the primary antibody incubation ...
-
bioRxiv - Microbiology 2023Quote: Lung single-cell suspensions were incubated with TruStain FcX™ (anti-mouse CD16/32) antibody (Cat: 101320; BioLegend, San Diego, CA) in eBioscience™ Flow Cytometry Staining Buffer (Cat ...
-
bioRxiv - Molecular Biology 2023Quote: WT and dcp2Δ strains were cultured in triplicate in YPD medium to A600 of 0.6–0.8 and treated with formaldehyde as previously described (Qiu et al. 2016). ChIP-Seq was conducted as described (Qiu et al. 2016) using monoclonal antibody against Rpb1 (8WG16, Biolegend, 664906). DNA libraries for Illumina paired-end sequencing were prepared using the DNA Library Prep Kit for Illumina from New England Biolabs (E7370L) ...
-
bioRxiv - Neuroscience 2023Quote: ... libraries were prepared according to the Chromium Single Cell 3ʹ with Feature Barcoding technology for Cell Surface Protein Reagent Kit v3 protocol (10X Genomics) with modifications for incorporation of TotalSeq™-A HTO antibodies (clones M1/42; 30-F11, BioLegend). Briefly ...
-
bioRxiv - Cancer Biology 2022Quote: ... the same number of cells (106 cells/100 µL) were stained with specific antibodies of CD31 (EC marker, eBiosciences 14-0311082) and CD45 (immune cell marker, BioLegend 103108) for 30 min on ice ...
-
bioRxiv - Microbiology 2023Quote: ... the cells were incubated with Alexa Fluor® 488-labeled anti-human CD69 antibody or an isotype control (1 µg/ml, BioLegend) for 45 min ...
-
bioRxiv - Microbiology 2023Quote: ... The following antibodies were used for flow cytometry analysis: FITC-conjugated anti-CD19 (6D5) (BioLegend, San Diego, CA, USA, cat# 115505), APC-conjugated anti-CD11b (M1/70 ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were centrifuged at 1000 rpm and resuspended in flow buffer containing the primary antibodies: rat GFP (Biolegend, 338001, 1:200), guinea pig MAP2 (Synaptic Systems ...
-
bioRxiv - Biophysics 2023Quote: ... biotinylated anti-mouse CD3e (Cat# 100304) and FITC anti-mouse TCRb antibody (Cat# 109206) were purchased from BioLegend (San Diego, CA). Azide-PEG4-NHS ester (Cat# AZ103-100 ...
-
bioRxiv - Immunology 2023Quote: ... CAR-transduced (thus EGFRt+) T cells were detected by staining with anti-EGFR antibody (clone AY13, Biolegend, San Diego, CA, USA) and scFv-Linker (custom made ...