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Citations for Roche :
651 - 700 of 3681 citations for rno mir 32 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... All primers were used together with LightCycler 480 SYBR Green I Master (Roche). For mitochondrial analysis we used previously published primers used for mitochondrial copy number assessment (MtDNA ...
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bioRxiv - Immunology 2020Quote: ... Primers were designed using LightCycler Probe Design Software (version 2.0; Roche Applied Science) or the free online Primer3 software (version 0.4.0 ...
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bioRxiv - Immunology 2022Quote: ... and the primers shown in Table S5 on the Light Cycler 480II (Roche) and gene expression was determined and normalized to Hprt using the 2-ΔCt method.
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bioRxiv - Developmental Biology 2022Quote: ... including T7 promoters in the primers and subsequent transcription with T7 Polymerase (Roche). Primer sequences used are found in table 1 ...
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bioRxiv - Molecular Biology 2022Quote: ... using the specific primers and LightCycler-DNA Master SYBR Green I (Roche Diagnostics) or with ABI Prism 7900HT Sequence Detection System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... using 0.20 uM of primers and KapaSYBR FAST qPCR master mix (Kapa Biosystems).
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bioRxiv - Molecular Biology 2023Quote: ... and 200 nM primers in a LightCycler 96 (Roche Life Science, Basel, Switzerland). Cycling conditions were 95 °C for 3 min ...
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bioRxiv - Systems Biology 2023Quote: ... a qPCR was performed using the KAPA Library Quantification Standards with Primer (Roche Sequencing Solutions Inc ...
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bioRxiv - Developmental Biology 2023Quote: ... Barcoding/adapter primers (Table S7) and KAPA™ HiFi HotStart Ready Mix (Roche) were used to amplify libraries ...
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bioRxiv - Neuroscience 2023Quote: ... Primers were designed using the Universal ProbeLibrary Assay Design Center (Roche, Tab. 2). Transcript levels of candidate genes were measured by qRT-PCR using cDNA with the QuantStudio 6 Flex Real-Time PCR System (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... using a 1:1 mixture of oligo dT and random hexamer primers (Roche). Quantification was performed on a LightCycler 480 (Roche) ...
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bioRxiv - Plant Biology 2020Quote: ... amplification and sequencing was performed by Novogene (Hong Kong) using the SeqCapEZ probe set 140430_Wheat_TGAC_D14_REZ_HX1 for Triticum aestivum (Roche, Nimblegen, WI, USA), described by Krasileva et al ...
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bioRxiv - Genomics 2022Quote: ... Bait sets were amplified in reactions containing 12.5 μl of 2X KAPA HiFi HotStart Polymerase Mix (7958927001, Roche), 0.75 μl of each 10 μM primer ...
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bioRxiv - Genetics 2020Quote: ... PCR products were purified with High Pure PCR Product Purification Kit (Roche). After sequencing reaction ...
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bioRxiv - Cell Biology 2020Quote: ... PCR was performed using KAPA2G Robust Hotstart PCR kit (KAPA Biosystems, #KK5518), following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... PCR amplification was performed using KAPA HiFi PCR Mix (Kapa Biosystems KK2602). Specifically ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR was performed with a KAPA HiFi HotStart ReadyMix PCR Kit (Roche) to add Illumina sequencing adapters (P5-dual-index and P7-dual-index ...
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bioRxiv - Bioengineering 2022Quote: ... PCR was performed using 12.5 μl KAPA2G Fast PCR mastermix (KAPA Biosystems), containing reaction buffer ...
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bioRxiv - Immunology 2020Quote: ... qRT-PCR was performed using the LightCycler 480 II PCR System (Roche) with program as followed ...
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bioRxiv - Immunology 2020Quote: ... PCR amplification of the pools with the KAPA HiFi PCR Kit (Roche) was done with 2.5 uL of a 2 ng/uL solution of the pool in a 50uL reaction ...
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bioRxiv - Neuroscience 2019Quote: ... qRT-PCR was performed using the SYBR Green PCR Master Mix (Roche) on a ViiA7 Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Plant Biology 2019Quote: ... Genotyping PCR was performed using KAPA Taq Extra PCR kit (Kapa Biosystems) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2019Quote: ... Quantitative PCR (qRT– PCR) was performed on a 480 LightCycler thermocycler (Roche) using the manufacturer’s instructions with Light cycler 480sybr green I master (Roche ...
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bioRxiv - Microbiology 2021Quote: ... Real-time PCR was carried out using SYBR Green PCR mix (Roche) in Roche LightCycler 480II Instrument.
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bioRxiv - Microbiology 2019Quote: ... A second PCR step using the Expand High Fidelity PCR System (Roche) allowed the incorporation of the Illumina MiSeq version 2 indexes and adapters ...
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bioRxiv - Immunology 2021Quote: ... qRT-PCR was performed using the LightCycler 480 II PCR System (Roche) with the following program ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative PCR was performed using Kapa SYBR fast PCR kit (Kapa Biosystems) in a Realplex 4 PCR machine (Eppendorf) ...
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bioRxiv - Immunology 2022Quote: ... Quantitative real-time PCR (qRT-PCR) was performed with SYBR green (Roche) in a StepOnePlus system (Applied Biosystems) ...
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bioRxiv - Microbiology 2022Quote: ... PCR was carried out with the KAPA HiFi PCR Kit (KAPA Biosystems) in a total volume of 25 μl ...
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bioRxiv - Systems Biology 2023Quote: ... Real-time PCR was conducted using the SYBR green PCR reagent (Roche) and StepOnePlus™ System (Applied Biosystems ...
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bioRxiv - Bioengineering 2024Quote: ... and PCR amplification was performed with KAPA2G Robust PCR kits (KAPA Biosystems) as described above ...
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bioRxiv - Developmental Biology 2021Quote: ... RT-qPCR was performed using KAPA SYBR® FAST Master Mix (KAPA Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and library concentration was quantified with KAPA Library Quant KIT RT-qPCR (Roche). Total RNAseq was done as 100nt paired-end sequencing and performed using the Illumnia platform (HiSEQ4000 ...
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bioRxiv - Genomics 2020Quote: ... RT-qPCRs were performed using 1× LightCycler 480 SYBR Green I Master (Roche), 0.5 μM primers (Suppl ...
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bioRxiv - Genomics 2021Quote: ... and by RT-qPCR using the KAPA library quantification kit (#KK4835, Kapa Biosystems), according to the manufacturers’ protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... RT-qPCR was performed on a LightCycler® 480 II (Roche Life Science) using SYBR® Premix Ex Taq(tm ...
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bioRxiv - Developmental Biology 2022Quote: ... RT-qPCR was performed using KAPA SYBR FAST qPCR Kit (Kapa Biosystems, KK4602) according to manufacturer’s instructions with the following cycler programme ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-qPCR was subsequently performed as described [23] using Sybr green (Roche, 06924204001) on The LightCycler 480 System ...
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bioRxiv - Genetics 2022Quote: ... RT-qPCR was performed using the Kapa SYBR FAST qPCR kit (Kapa Biosystems) and StepOnePlus™ Real-time PCR System (Applied Biosystems ...
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bioRxiv - Genomics 2022Quote: ... RT-qPCR was performed using LightCycler 480 SYBR Green I master mix (Roche). For quantification of gene expression ...
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bioRxiv - Immunology 2022Quote: ... slides were blocked at RT for 1 h in 1x Blocking reagent (Roche) and incubated at 4°C over night with a phospho-histone H2AX (pSer139 ...
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bioRxiv - Plant Biology 2022Quote: ... RT-qPCR assays were carried out on LightCycler LC480 II (Roche Diagnostics, Germany) using SYBR Green Jumpstart Taq Ready mix (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR was performed using KAPA SYBR FAST qPCR master mix (Kapa Biosystems) with gene-specific primers (Supplementary Table 6 ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR was performed in a LightCycler® 2.0 instrument (Roche, Mannheim, Germany), using 45 cycles of the following program ...
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bioRxiv - Physiology 2020Quote: ... Reverse transcription was performed with RT Transcriptor First Strand cDNA Synthesis Kit (Roche) with anchored oligo-dT primers (Roche) ...
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bioRxiv - Genetics 2019Quote: ... RT-qPCR reactions were carried out in quadruple on a LC480 machine (Roche). The reaction mix consisted of cDNA ...
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bioRxiv - Molecular Biology 2022Quote: ... a minimum of 50ng of RNA was used to prepare cDNA (Roche RT) and splice isoform specific PCRs were performed (Sigma Aldrich High fidelity Taq ...
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bioRxiv - Immunology 2023Quote: ... The RT-qPCR was run on a Roche LightCycler 480 (Roche Diagnostics Ltd) using the conditions outlined in Table 4.
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bioRxiv - Biophysics 2022Quote: GGCAGCGCTACCATAACGGA-3’) (11) by RT-qPCR was performed using LightCycler 480 II (Roche). GAPDH mRNAs were used as a loading control (14) ...
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bioRxiv - Plant Biology 2022Quote: ... Expression analysis by RT-qPCR was performed using SYBR Green master I (Roche) and the LightCycler® 96 system following a standard protocol (40 cycles ...