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Citations for Roche :
501 - 550 of 3681 citations for rno mir 32 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... qPCR reactions were set with Light Cycler 480 SYBR Green I Master mix (Roche), 2 μl of cDNA and 0.3 μM primers (Extended Data Table 3) ...
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bioRxiv - Plant Biology 2021Quote: ... WTK4 transcript accumulation was confirmed by PCR amplification using the primers JS693xJS671 followed by a semi-nested reaction with primers JS696xJS671 using KAPA Hifi HotStart Polymerase (KK2502, Kapa Biosystems, Hoffmann-La Roche) with an annealing temperature of 60°C and extension time of 3 min ...
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bioRxiv - Molecular Biology 2024Quote: RNA was extracted from Lipo246 and Lipo815 cell lines and Reverse transcription (RT) reactions were carried out using Transcriptor RT enzyme (Roche Diagnostics, Basel, Switzerland). Nested PCR was performed using a 2-step PCR reaction as previously described(31).
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bioRxiv - Genomics 2019Quote: RNA primers were removed with RNAse A/T1 Mix (Roche) for 60 min at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... with primers (Oligomer) and probes (Oligomer or Roche Applied Science) reported in Vihervaara et al ...
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bioRxiv - Immunology 2020Quote: ... Gene-specific primers were designed using Universal Probe Library (Roche) and real-time PCR performed using GoTaq qPCR Master mix (Promega ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was synthesized with random p(dN)6 primers (Roche) and MMLV reverse transcriptase (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... and 0.3 μg/uL of random primers p(DN) (Roche) at 65°C for 5 min ...
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bioRxiv - Physiology 2020Quote: ... Primers were designed using the ProbeFinder software (Roche Applied Sciences). qRT-PCR was performed in triplicate with a MyiQ single-color real-time PCR detection system (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was synthesized with random primers p(dN)6 (Roche) using SuperScript III Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Genetics 2021Quote: ... livers were perfused at low flow for approximately 10 min with Digestion Buffer (low glucose DMEM supplemented with 1 mM HEPES) containing freshly added Liberase TM (32 μg/mL; Roche). Digestion was stopped using Isolation Buffer (low glucose DMEM supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... at 1:500 for 32 min was used followed with the secondary antibody OmniMap anti-rabbit HRP (760-4311, Roche). Antigen-antibody complexes were revealed with ChromoMap DAB Kit (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... The 12 and 32 libraries were equimolarly pooled and subjected to qPCR quantification using the KAPA library quantification kit (Roche). Sequencing was carried out on a NovaSeq 6000 instrument (Illumina ...
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bioRxiv - Bioengineering 2024Quote: ... livers were perfused at low flow for about 10 min with digestion buffer (low-glucose DMEM supplemented with 1 mM Hepes) containing freshly added Liberase (32 μg/ml; Roche). Digestion was stopped using isolation buffer (low-glucose DMEM supplemented with 10% FBS) ...
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bioRxiv - Molecular Biology 2019Quote: ... The RT-qPCR was run on a LightCycler 480 (Roche). GAPDH primers were used as an internal control ...
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bioRxiv - Genomics 2021Quote: ... RT-qPCR were performed on the Light Cycler 480II (Roche) with the primers listed in Supplementary Data 6 using Eef1a1 and Hprt as housekeeping controls.
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bioRxiv - Cancer Biology 2021Quote: ... RT-qPCR was carried out with SYBR Green I (Roche) and SuperScript IV Reverse Transcriptase (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... Subsequent RT-qPCR was performed on a LightCycler96 platform (Roche) based on SARS-CoV-2 N gene RNA amplification using forward (5’-GACCCCAAAATCAGCGAAAT ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR was performed on a LightCycler 480 II (Roche) using LightCycler 480 SYBR Green I Master mix (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed in a LightCycler480 (Roche, Mannheim, Germany) using KAPA SYBR FAST Kit (KK4611 ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR was performed using the SYBR Green kit (Roche) with 100 nM primers and 0.125 μL of RT reaction product in a total volume of 5 μL per reaction ...
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bioRxiv - Microbiology 2019Quote: ... respectively with and without RT on a LightCycler 480 (Roche).
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bioRxiv - Genetics 2020Quote: ... RT-qPCRs were performed on the Light Cycler 480II (Roche) using Eef1a1 and Hptr as housekeeping genes ...
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bioRxiv - Evolutionary Biology 2021Quote: ... In the following RT qPCR with a Light-Cycler480 (Roche) and Kapa SYBR Fast (Kapa Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... The RT-qPCR was conducted using LightCycler 480 Instrument (Roche) using the following conditions ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR was performed using a Light Cycler 480 (Roche). Ten RNA copies was the lowest number used to calculate the standard curve.
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bioRxiv - Immunology 2021Quote: ... RT-qPCR was performed using a Light Cycler 480 (Roche). Changes in relative gene expression were calculated according to the 2-ΔΔCT method (43).
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bioRxiv - Plant Biology 2022Quote: ... RT-qPCRs were performed using SYBR® Green Mix (Roche) in a LightCycler® 480 (Roche) ...
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ENGRAILED-1 transcription factor has a paracrine neurotrophic activity on adult spinal α-motoneuronsbioRxiv - Neuroscience 2023Quote: ... RT-qPCR was done using SYBR-Green (Roche Applied Science) and a Light Cycler 480 (Roche Applied Science) ...
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bioRxiv - Plant Biology 2023Quote: ... KAPA SYBR FAST One-Step RT-qPCR kit (Roche, KK4650) was used following manufacturer protocols ...
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bioRxiv - Plant Biology 2023Quote: ... RT-qPCR reactions were realized on LightCycler® 480 (Roche) with MESA FAST qPCR MasterMix Plus for SYBR® Assay No ROX (Eurogentec) ...
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bioRxiv - Cell Biology 2024Quote: ... RT-qPCR was performed on a LightCycler 480 instrument (Roche) using SYBR Green I Master Mix (4887352001 ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 1% protease inhibitor cocktail set III ethylenediaminetetraacetic acid(EDTA)-free (Roche, Shanghai, China). Protein concentrations were determined using a Pierce BCA Protein Assay Kit (Lineage Cell Therapeutics ...
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bioRxiv - Microbiology 2020Quote: Sigmoid biopsies (≈32 biopsies/donor) were collected from HIV-infected individuals receiving ART during colonoscopy and processed using Liberase DL (Roche Diagnostics), as previously described (28) ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by rabbit anti-Rat (AI-4001, Vector) at 1:500 for 32 min and OmniMap™ anti-Rb HRP (760-4311, Roche). Blocking was performed using casein (760-219 ...
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bioRxiv - Microbiology 2022Quote: ... incubated overnight at 32°C and replica-plated on a YES agar plate containing 100 mg/L hygromycin B (Roche, #10843555001). The hygromycin plates were incubated for 1-2 days at 32°C until colonies had formed ...
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bioRxiv - Microbiology 2021Quote: ... using the primers indicated in Table 1 after cDNA synthesis using random hexamer primers and Roche AMV reverse transcriptase (10109118001 Roche, Sigma-Aldrich, St. Louis, MO, USA). Samples were multiplexed using Takara long amplicon Taq polymerase with GC buffers following the manufacturer’s instructions for PCR barcoding for nanopore sequencing (EXP-PBC096 Oxford Nanopore Technologies ...
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bioRxiv - Genomics 2019Quote: ... Primer extension was performed by incubation with 10 mM dNTPs (Roche) and 5 U exo-Klenow Fragment (New England Biolabs ...
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bioRxiv - Bioengineering 2019Quote: ... Primer sequences (S1 Table) were designed with the ProbeFinder software (Roche), and all reactions were run in triplicate.
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bioRxiv - Bioengineering 2020Quote: ... Primer pairs were designed using the Universal Probe Library by Roche (https://lifescience.roche.com/en_de/brands/universal-probe-library.html#assay-design-center ...
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bioRxiv - Immunology 2022Quote: ... Primers were designed using Roche Universal Probe Library (Roche, Basel, Switzerland). The sequences of the specific primers are as follows ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.25µl l0mM reverse primer) was run on a Lightcycler 480 (Roche) using the program ...
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bioRxiv - Immunology 2022Quote: ... Primers and probes were chosen from the Universal Probe library (Roche). The fold change in gene expression was determined by the ΔΔCT method ...
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bioRxiv - Genetics 2019Quote: ... were designed in Primer Express 3.0 (Applied Biosystems-Roche, Branchburg, NJ). Including primers ...
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bioRxiv - Pathology 2021Quote: ... Specific primers (table 1) were designed using Probe Design (Roche Diagnostics).
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bioRxiv - Genomics 2021Quote: ... qPCR was performed using validated primers and SYBR Green Mix (Roche) in 384-well plates on a LightCycler480 device (Roche ...
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bioRxiv - Immunology 2021Quote: ... cDNA was amplified with primers and FastStart SYBR Green Mastermix (Roche) using QuantStudio 6 Flex (Applied Biosystems) ...
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bioRxiv - Plant Biology 2023Quote: ... together with the primers from the Kapa Hyper Prep kit (Roche) and MyTaq Master mix (Bioline) ...
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bioRxiv - Plant Biology 2023Quote: ... together with the primers from the Kapa Hyper Prep kit (Roche) and MyTaq Master mix (Bioline ...
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bioRxiv - Plant Biology 2023Quote: ... Oligo dT18 primer and Transcriptor First Strand cDNA synthesis kit (Roche) as per the manufacturer’s instructions ...