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Citations for Roche :
601 - 650 of 3681 citations for rno mir 32 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Gene-specific primers and universal probes were designed using ProbeFinder software (Roche). The primer and probe sequences for mAPOBEC1 were 5’- CAGCGGTGTGACTATCCAGA-3’ ...
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bioRxiv - Cancer Biology 2023Quote: ... and KAPA Library Amplification Primer Mix (10X concentration, Roche, cat. no. KK2623), 10 µL library input each with 11 cycles and 9 min extension time ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Sequence-specific primers and probes for each gene were procured from Roche Applied Science ...
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bioRxiv - Genomics 2020Quote: ... PCRs were performed using KAPA2G Robust PCR kits (KAPA Biosystems). A typical 25 μl reaction used 20–100 ng of genomic DNA ...
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bioRxiv - Plant Biology 2020Quote: ... PCR used the Kapa Taq PCR kit from Kapa Biosystems (Wilmington ...
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bioRxiv - Systems Biology 2021Quote: ... Barcoding PCR was done by KAPA HiFi PCR Kit (Roche) in a final 25μl reaction (11.5μl H2O ...
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bioRxiv - Developmental Biology 2020Quote: ... The PCR kit used was FastStrat PCR Master (Roche, 04710436001). The amplification product from mutated allele has a 341bp size and the one obtained from a wild-type allele is 262bp ...
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bioRxiv - Cancer Biology 2023Quote: ... Barcoding PCR was done using KAPA HiFi PCR Kit (Roche) in a final volume of 25 μl ...
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bioRxiv - Microbiology 2020Quote: ... the sections were incubated with a primary antibody for 32 min at room temperature and then incubated with the reagent from an iVIEW DAB detection kit (Roche Diagnostics, Meylan, France). The sections were counterstained with hematoxylin and post-counterstained with bluing reagent ...
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bioRxiv - Genetics 2020Quote: ... PCR KIT (Roche). For each reaction ...
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bioRxiv - Neuroscience 2019Quote: ... and incubated in HNPP/Fast red working solution according to manufacturer’s instructions (HNPP Fluorescent Detection Set, Roche). To stop the reaction ...
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bioRxiv - Genomics 2020Quote: ... subsequent washes were performed using the DIG Wash and Block Buffer Set (Roche # 11 585 762 001). Anti-Digoxigenin-AP Fab fragments were used at 1:10,000 ...
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bioRxiv - Microbiology 2020Quote: ... DIG Washing and DIG Blocking Buffers were prepared using the DIG Wash and Block Set (Roche, USA). The blot was washed twice with Washing Buffer for 10 minutes at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... the bacteria were lysed in a PBS-based buffer containing a complete set of protease inhibitors (Roche), binding to the Ni-NTA resin was carried out in the presence of 10 mM imidazole ...
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bioRxiv - Molecular Biology 2021Quote: ... The bacteria were lysed in a PBS-based buffer containing a complete set of protease inhibitors (Roche). In a gravity-flow column ...
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bioRxiv - Genomics 2020Quote: ... This final probe set was used for the preparation of a SeqCap EZ Developer assay by Roche NimbleGen Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... reactions were set up at 10 µl reaction volume using LightCycler 480 SYBR Green Master Mix (Roche) and performed using the LightCycler 480 Real-Time PCR Instrument (Roche) ...
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bioRxiv - Developmental Biology 2024Quote: ... qPCR reactions were set up in technical duplicates using LightCycler 480 SYBR Green I Master Mix (Roche), 5 ng amplified DNA and 1 μM forward and reverse primers and run on a LightCycler 480 machine (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 h at RT and 20 μg/mL Laminin (Roche, Switzerland) for 4 h at 37 °C ...
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bioRxiv - Genetics 2022Quote: ... The RT-qPCR was done in duplicate on a Lightcycler 480 (Roche) with SYBR green for detection (2,5 μl of mastermix ...
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bioRxiv - Physiology 2021Quote: ... RT-qPCR was performed in duplicate with the LightCycler 480 (Roche Diagnostics) instrument using LightCycler 384-well plates with sealing foil (Roche) ...
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bioRxiv - Cancer Biology 2020Quote: ... The RT-qPCR cycling analysis was performed by LC-480 device (Roche). qBasePlus software 3.2 (http://www.biogazelle.com ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed with the Light Cycler 96 (Roche, Geneva, Switzerland) using iQ SYBR Green Supermix (170-8882AP ...
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bioRxiv - Cancer Biology 2022Quote: ... RT-qPCR was performed using SYBR Fast qPCR Master Mix (Kapa Biosystems) on a Light Cycler 480 (Roche) ...
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bioRxiv - Evolutionary Biology 2019Quote: Dengue virus was quantified via RT-qPCR using the LightCycler 480 (Roche). We used the TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Following quantification by RT-qPCR using KAPA Library Quantification Kit (KAPA Biosystems), libraries were pooled in equimolar concentrations for cluster generation on cBOT system (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... reverse-transcribed (RT) using a Transcriptor First Stand cDNA synthesis kit (Roche) and quantified on Quantitative PCR Q7 Detection System (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... pH 7.4 at room temperature (RT)) supplemented with protease inhibitor cocktail (Roche) and Benzonase (Merck ...
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bioRxiv - Plant Biology 2022Quote: ... The RT-QPCRs were performed using a LightCycler480 machine (Roche; Mannheim, Germany) and data were extracted using LightCycler480 software ...
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bioRxiv - Plant Biology 2023Quote: ... RT-qPCR reactions were performed on a LightCycler 96 (Roche, Mannheim, Germany) using qPCRBIO SyGreen Mix (PCR Biosystems ...
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bioRxiv - Plant Biology 2023Quote: ... RT-qPCR was performed using LightCycler 480II Real Time system (Roche, USA) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... RT-qPCR is performed using The LightCycler 480 SYBR Green I (Roche), and the reaction was run at LightCycler 480 Instrument II (Roche) ...
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bioRxiv - Synthetic Biology 2023Quote: ... We analysed RT-qPCR reaction using the LightCycler® 96 Software (Roche). We calculated viral genome concentration (Vg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR was run on a LightCycler 480 II (Roche Applied Science) using 384 well plates (see RT-qPCR section for thermocycling details) ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR was run on a LightCycler 480 II (Roche Applied Science) using 384 well plates ...
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bioRxiv - Synthetic Biology 2023Quote: ... We measured RT-qPCR reaction on a LightCycler® 96 Instrument (Roche) using the SYBR-green channel and with cycling conditions as follows ...
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bioRxiv - Physiology 2024Quote: ... RT-qPCR was performed in duplicate with the LightCycler 480 (Roche Diagnostics) instrument using LightCycler 384-well plates with sealing foil (Roche) ...
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bioRxiv - Genetics 2021Quote: ... PCR reactions with KAPA HiFi HotStart ReadyMix PCR Kit (Kapa Biosystems) were setup with 80ng genomic DNA ...
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bioRxiv - Bioengineering 2024Quote: ... PCR was performed using KAPA2G Robust PCR kits from Kapa Biosystems. The 25 µL reactions used 25 ng of cDNA ...
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bioRxiv - Systems Biology 2020Quote: ... All primers were used together with LightCycler 480 SYBR Green I Master (Roche). Standard procedures of qRT-PCR were used ...
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bioRxiv - Microbiology 2020Quote: ... and cDNA was synthesized with SuperScript II using Oligo dT primers (Roche Diagnostics). Quantitative PCR was performed using previously described gene specific primers in an ABI7300 Real Time PCR system with SYBR Green Master Mix (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... and 800 nM of each primer in a LightCycler 480 System (Roche Diagnostics). RT-qPCR was run in two biological replicates with three technical replicates each and primers are shown in S7 Table ...
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bioRxiv - Cell Biology 2020Quote: ... Primer-probe combinations were selected based on the Universal Probe Library System (Roche) and are listed in Supplementary table 2 ...
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bioRxiv - Cancer Biology 2021Quote: ... and final molarity quantification determined by qPCR with adapter specific primers (Kapa Biosystems) on a Roche Light Cycler ...
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bioRxiv - Neuroscience 2021Quote: ... All primers were used together with LightCycler 480 SYBR Green I Master (Roche). Three reference genes were used for each qRT-PCR analysis (ACTB ...
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bioRxiv - Neuroscience 2021Quote: ... All primers were used together with LightCycler 480 SYBR Green I Master (Roche). Standard procedures of qRT-PCR were used ...
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bioRxiv - Molecular Biology 2022Quote: ... with primers listed in Table S3 on a LightCycler® LC480 instrument (Roche). Primer efficiencies were > 95% and calculated on standard curves generated by a 2-fold dilution series of cDNAs over at least five dilution points measured in triplicate ...
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bioRxiv - Plant Biology 2021Quote: ... with gene-specific primers (Supplementary Table 6) on a LightCycler 480 system (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... and specific primers near the ends as recommended by the manufacturer (Roche, Germany). Sequence comparisons ...
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bioRxiv - Developmental Biology 2021Quote: ... with specific primers listed in Supplementary Table 1 and a LightCycler 480 (Roche). Samples were run in triplicate and gene of interest expression was normalized to human Gapdh or mouse Rplp0.