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Citations for Roche :
301 - 350 of 1110 citations for Rnase 3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Human fibronectin (Roche Diagnostics, 11051407001); Puromycin (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... and human insulin (11376497, Roche) were digested with recombinant WT or protease-dead (cf-E111Q ...
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bioRxiv - Biophysics 2021Quote: ... 3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate (CHAPS, Roche Life Sciences, Indianapolis, IN) micelles and also using 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC)/1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC ...
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bioRxiv - Immunology 2023Quote: ... 3 mM ATP (Roche), 25 μg/ml MSU (InvivoGen) ...
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bioRxiv - Bioengineering 2019Quote: ... DNA was amplified using the polymerase Kapa Hi Fi DNA polymerase (Kapa Biosystems, cat no. KK2602) and gel purified using Seakem GTG agarose (for nucleic acid recovery ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting Hi-C library was amplified by PCR (KAPA Biosystems HiFi HotStart PCR kit, KK2502), and sequenced by Illumina 50 bp paired-end sequencing.
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bioRxiv - Biophysics 2020Quote: ... and a biotin molecule (Supplementary Note 1) via PCR amplification with KAPA Hi Fi Polymerase (Roche) and nicking near the ends by the site-specific nicking enzyme Nt.BspQ1 (NEB) ...
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bioRxiv - Biochemistry 2022Quote: ... lysed by sonication as described above and loaded onto the cOmplete His-tag purification column (Roche). The complex was eluted in 300 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified cell lysates were incubated with 10 mL of cOmplete His-Tag purification resin (Roche, Germany) for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... The protein eluted after immobilized metal affinity chromatography (IMAC) using complete His-tag purification resins (Roche) was concentrated using 10KDa cut-off amicon filters (Merck ...
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bioRxiv - Molecular Biology 2023Quote: ... and filtered prior to loading onto a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Hi-C next-generation sequencing (NGS) libraries were prepared using the KAPA HyperPlus Kit (KAPA Biosystems) according to instructions 129.
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bioRxiv - Evolutionary Biology 2023Quote: ... the supernatant was further incubated with 25 μl of cOmplete His-tag purification resins (Roche, Switzerland) at 4 °C for 2 h ...
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bioRxiv - Cell Biology 2024Quote: ... the cleared lysate was added to 1 mL cOmplete His-Tag Purification Resin (COHISR-RO, Roche) that had been pre-washed with buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... The extracted RNA was then treated with DNase I recombinant RNase free (Roche) and the reverse transcription was done using the Super-Script IV (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were clarified by centrifugation and treated with RNAse A (Roche, 100 Units) or RNAse A/T1 (NEB ...
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bioRxiv - Molecular Biology 2019Quote: ... 75μg of RNase I and 7μl of DNaseI (Roche, of 10U/μl stock). The cells were then sonicated at 80% amplitude for 5 s/burst/35ml of suspension using a Sonics Vibra-Cell (3mm microtip) ...
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bioRxiv - Microbiology 2020Quote: ... The RNA was treated with RNase-free DNase I (Roche, Cat. No.: 04716728001) and stored at −80 °C.
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bioRxiv - Developmental Biology 2020Quote: ... we used 1 mg/ml of RNase in PBS (10109169001, Roche, Basel, Switzerland). At 0 hours and 18 hours after creation of the migration zone ...
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bioRxiv - Cancer Biology 2021Quote: ... post-transduction from three wells and treated with RNAse-free DNAse I (Roche) for 1 hour at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... The RNA was then dissolved and treated with RNAse-free DNase I (Roche) for 20 minutes at 37°C ...
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bioRxiv - Microbiology 2023Quote: DNase treatment was performed using recombinant RNase-free DNase I (Roche, Basel, Switzerland) according to the manufacturer’s protocols for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... 40 U of RNase inhibitor and with cOmplete TM Protease Inhibitor Cocktail (Roche). For the input for Western blot analysis ...
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bioRxiv - Cancer Biology 2022Quote: DNA probes were digested with 0.5U/ml RNase-free DNase I (Roche, #04716728001) for 1h at RT to perform a multi-day hybridization routine ...
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bioRxiv - Microbiology 2019Quote: ... Human genomic DNA (Roche Cat#11691112001) was purchased from Sigma (Sigma-Aldrich).
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bioRxiv - Microbiology 2019Quote: ... Human genomic DNA (Roche Cat#11691112001) was purchased from Sigma (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2020Quote: ... 180 g/ml human transferrin (Roche), 5 ng/ml VEGF (PeproTech 450-32) ...
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bioRxiv - Immunology 2022Quote: ... recombinant human IFNγ from Roche (#11040596001), recombinant human IL-1β from PeproTech (#200-01B).
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bioRxiv - Cancer Biology 2020Quote: ... 180 μg/ml human transferrin (Roche). Flk-1+ cells were isolated by magnetic cell sorting (MACS ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag fusion proteins were purified with a cOmplete™ His-tag purification resin (Roche, Merck). The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2024Quote: ... The expression of the 35S-driven IAR3 gene was monitored by protein immunodetection with anti-His (Roche) and anti-mouse IgG-peroxidase (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... All the signals were visualized by adding 3-3′-Diaaminobenzidinetetrahydrochloride (DAB substrate) solution (Roche) to the slides and counterstained with haematoxylin ...
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bioRxiv - Biophysics 2020Quote: ... 3.5 μg A3H was or was not treated with RNase A (Roche Applied Science) for 15 min at room temperature ...
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bioRxiv - Genetics 2021Quote: ... followed by addition of 5 µl of 4 µg/mL DNase-free RNase (Roche). Samples were loaded onto 96-well Qiacube-HT® columns and DNA was purified using a Blood & Tissue kit (Qiagen) ...
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bioRxiv - Genetics 2020Quote: ... The eluted samples were then treated with RNase A (Wako) and Proteinase K (Roche), and purified using a PCR purification kit (NEB).
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bioRxiv - Cell Biology 2021Quote: ... The virus was pretreated with 20 U ml-1 RNase-free DNase I (Roche) for 1 h at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA was degraded by incubation with 2 μL RNase A (10 mg/mL; Roche) for 1 h at 37°C and protein was removed by incubation with 10 μL of proteinase K (18 mg/mL ...
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bioRxiv - Genetics 2019Quote: ... cells were washed twice with PBS and treated with RNase (Roche, Cat. No. 11119915001) for 30 minutes at 37°C ...
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bioRxiv - Genetics 2019Quote: ... Cells were washed twice with PBS and treated with RNase (Roche, Cat. No. 11119915001) for 30 minutes at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... DNA from lysed cells was removed using 5 μl RNAse-free DNAse I (Roche) for every 1 ml dissociate and incubated at 37°C for 5 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.25 mM DTT) supplemented with 0.05 U/µl RNase-free recombinant DNase I (Roche), 0.4 U/µl RNasin (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclei were labeled with Hoechst dye and 0.2 U/μl Protector RNase inhibitor (Roche). The labeled nuclei were purified using FACS (BD Aria ...
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bioRxiv - Cell Biology 2023Quote: ... uuclei were labeled with Hoechst dye and 0.2 U/μl Protector RNase inhibitor (Roche). All GFP+ Hoechst stained myonuclei were purified using FACS (BD Aria ...
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bioRxiv - Microbiology 2022Quote: ... Ribosomal RNA was removed by an Rnase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94°C for 8 min ...
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bioRxiv - Microbiology 2023Quote: ... Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94°C for 8 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Ribosomal RNA was removed by an RNase-H method using RiboErase kit (Kapa Biosystems). After rRNA depletion ...
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bioRxiv - Cancer Biology 2024Quote: ... resuspended in PBS containing 0.2 mg/ml DNAse free RNAse A (Roche, Basel, Switzerland), and incubated for 1 h at 37ºC ...
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bioRxiv - Neuroscience 2024Quote: ... supernatant was aspirated and the nuclei resuspended in 1x PBS and RNAse inhibitor (Roche, RNAse inhibitor concentration according to suppliers’ recommendation) ...
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bioRxiv - Cancer Biology 2024Quote: ... buffer supplemented with 0.7% CHAPS (Thermo #28299) and 0.2U/μl RNase inhibitor (Roche #3335399001). Hepatocytes were lysed on ice for 5 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... 5’/3’RACE Kit (Roche) was used to amplify the RNA obtained and the final DNA was sequenced to obtain the +1 nucleotide of str ...