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Citations for Roche :
251 - 300 of 1110 citations for Rnase 3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... with the addition of 10 μg RNase-free glycogen (Roche #10901393001) in the isopropanol step ...
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bioRxiv - Genetics 2021Quote: ... The purified RNA was treated with DNase I (RNase-free, Roche) and used to produce oligo dT-primed cDNAs (SuperScript II RT ...
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bioRxiv - Neuroscience 2022Quote: ... followed by incubation with 1 mg/mL Rnase A (Roche, 10109142001) at 37°C for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... 0.2 mg/ml RNAse-free bovine serum albumin (BSA, Roche Diagnostics) and 10% dextran sulfate (MP Biomedicals) ...
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bioRxiv - Physiology 2022Quote: ... The purified RNA was treated with DNase I (RNase-free, Roche) and used to produce oligo dT-primed cDNAs (SuperScript II RT ...
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bioRxiv - Microbiology 2023Quote: ... phage lysates were treated with RNase A (Roche; 1 μg/mL), DNase I (NEB ...
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bioRxiv - Synthetic Biology 2023Quote: ... we added 1 uL RNase (from bovine pancreas, DNase free, Roche) and 1 uL RNase H (NEB ...
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bioRxiv - Microbiology 2024Quote: ... each sample was incubated with 10 μL of RNAse A (Roche Diagnostics GmbH ...
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bioRxiv - Biochemistry 2023Quote: ... the DNA was treated with 1 µg DNase-free RNase (Roche) for 30 minutes at 37°C ...
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bioRxiv - Genetics 2023Quote: ... extracted RNA was treated with RNase-free DNase I (Roche #04716728001) for 1 hour at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... containing 500µL ice-cold DPBS and 1µL Protector RNAse inhibitor (Roche). For subsequent analysis of mRNA expression via qPCR or bulk RNA sequencing ...
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bioRxiv - Molecular Biology 2019Quote: ... The supernatant was added to a slurry of cOmplete His-Tag Purification Resin (Roche) and incubated for 1 h at room temperature or 2 h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was added to a slurry of cOmplete His-Tag Purification Resin (Roche) or Ni Sepharose High Performance (Merck ...
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bioRxiv - Microbiology 2021Quote: ... Fab was purified from cell culture supernatant by cOmplete His-Tag Purification Resin (Roche).
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bioRxiv - Neuroscience 2023Quote: ... His6-GFP and His6-mCherry proteins were purified with cOmplete His tag resin (Roche) according to the manufacturers protocols as previously described9 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
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bioRxiv - Genomics 2024Quote: ... the dialyzed sample was loaded again onto a cOmplete His-tag purification column (Roche), and the untagged Tn5 was collected in the flow-through ...
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bioRxiv - Physiology 2020Quote: ... Patch-clamp experiments on HCN1 and HCN2 were performed with transient transfection of 2 μg of either pCDNA3.1-HCN1 or pCDNA3.1-HCN2 (generously provided by Dr. Eric Accili) in HEK293 cells using Fugene6 (Roche, Basal, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The pcDNA3 constructs were used for transient transfection in HEK293 cells using an X-tremeGENE HP DNA Transfection Reagent (Roche, 6366236001according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... anti-human CD4 clone SP35 and anti-human CD8 clone SP57 (Roche, Basel, Switzerland), and anti-human CD20 clone L26 Dako Omnis (Agilent ...
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bioRxiv - Molecular Biology 2020Quote: ... 5% CO2 for 24 h before transfection with a 4:1 DNA ratio of pCMV6-Affimer-tGFP and FLAG-ERK plasmids using Lipofectamine 2000 (SW620, HEK293, NCI-H460) or X-tremeGENE 9 (Roche; Panc10.05). After 24 h cells were serum starved for 1 h ...
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bioRxiv - Genetics 2020Quote: N-terminal HA-tagged PRUNE1 overexpression constructs were transfected into PRUNE1 non-expressing HEK293 cells using X-tremeGENE HP DNA (Roche; 06366236001) transfection reagent ...
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bioRxiv - Biochemistry 2019Quote: ... The transfection of GFP-RNase H1 R-loop-binding domain (GFP-HB) for R-loop detection into HEK293 cells was performed with the FuGENE Transfection reagent (Roche, E269A).
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bioRxiv - Cancer Biology 2023Quote: ... This plasmid was digested with PacI and transfected into HEK293 cells using X-tremeGENETM HP DNA transfection reagent (Roche, Basel, Switzerland) to produce infectious viral particles ...
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bioRxiv - Evolutionary Biology 2021Quote: ... followed by PCR amplification with KAPA 2X Hi-Fi Hotstart Readymix (Roche, Cat. No. KR0370).
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bioRxiv - Biochemistry 2019Quote: ... Clear lysate was affinity-purified by incubating it with cOmplete His-Tag purification resin (Roche) for 2 hours (4°C) ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... Expressed proteins were subsequently purified using a cOmplete His-Tag Purification column (1mL column, Roche) and SEC (S200 ...
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Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... The supernatants were then incubated with the Complete His-Tag Purification Resin (Roche, Basel, Switzerland) that had been prewashed with Renz buffer ...
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bioRxiv - Cell Biology 2024Quote: ... supernatants were harvested and purified using His-Tag purification resin (Roche, cOmpelteTM, Cat No.5893682001), followed by size-exclusion chromatography in 20 M Hepes or Tris pH 8 ...
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bioRxiv - Biochemistry 2021Quote: ... The clarified lysate was incubated with RNAse T1 (20 U/μL, Roche), RNase A (20 μg/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... The synthesized dsRNA was treated with TURBO DNase and RNase T1 (Roche) for 1 h at 37°C to remove DNA template and single-stranded RNA ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then treated with 25 μg/mL RNase A (10109142001, Roche) in PBS 1× at 37 °C for 20min ...
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bioRxiv - Cancer Biology 2021Quote: ... and 4 μL of 500 ug/ml RNase (Roche, Cat. No 11119915001) was added and incubated for 1 h at 37° C to digest RNA ...
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bioRxiv - Biochemistry 2020Quote: ... 0.1% NP-40) supplemented with 65 U RNase-free DNase I (Roche) and 208 U Superase•In™ RNase inhibitor (Invitrogen) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and treated with DNAse I (Dnase I recombinant RNAse-free, Roche Diagnostics). Then cDNA were produced from 5 μL of total RNA using RT Superscript III (RT Superscript III First Strand cDNA Synthesis Kit ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 µl of RNase-free glycogen (Roche, Welwyn Garden City, UK) was added to each sample as an inert carrier to assist RNA recovery ...
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bioRxiv - Cancer Biology 2022Quote: ... and stained with a solution of 100 μg/ml RNase (#10109142001, Roche) and 50 μg/ml propidium iodide (#P4170 ...
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bioRxiv - Molecular Biology 2022Quote: ... and 5 L of RNase A (20 mg/mL) (Roche Cat. 70294823) were added to flow cytometry samples and continued for incubation at room temperature for 30 minutes and protected from light ...
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bioRxiv - Molecular Biology 2021Quote: ... the lysates were treated with RNase-free DNase I (10 U, Roche) for 30 min at room temperature (RT) ...
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bioRxiv - Developmental Biology 2019Quote: ... after addition of CaCl2 (2mM final) and RNase A (Roche Diagnostics, #10109142001) treatment (75µg/ml final ...
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bioRxiv - Genomics 2020Quote: ... RNAs were eliminated by treating with 10 μg/ml RNase A (Roche) for 1 hr at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... the sample was treated with RNase free DNase (catalog no. 11119915001, Roche) as described previously (Knoll et al. ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was digested with DNase-free RNase (5 μg/ml, Roche #11119915001) for 1 hour at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... RNase (both at 20 µg/mL) and cOmplete protease inhibitor tablets (Roche). Cells were lysed by sonication at 70 W (3 s on ...
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bioRxiv - Molecular Biology 2023Quote: ... resuspended in TE 1X containing 10 μg.mL-1 RNase A (Roche, 10109169001) allowing the enzyme to act for a short incubation ...
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bioRxiv - Cancer Biology 2024Quote: ... The extracted DNA was RNAse treated (40 μg / ml) (Roche, Nutley, NJ) for 1 h at 37°C before being phenol chloroform extracted and ethanol precipitated ...
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bioRxiv - Cell Biology 2023Quote: ... and 2 μl of RNase-free DNase I (20 μ/ml, Roche) were added to the washed antibody beads and were precipitated ON rotating at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... fibronectin from human plasma (Roche), the final concentration used for the experiments was 50 μg/ml (containing 2/3 of bovine and 1/3 of human FN) ...
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bioRxiv - Developmental Biology 2019Quote: ... human holotransferrin 0.6% (Roche, 10652202001); monothioglycerol 0.0039 % (Sigma ...
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bioRxiv - Immunology 2019Quote: ... human recombinant IL-2 (Roche) was also added fresh immediately prior to use at a final concentration of 100 IU/ml ...