Labshake search
Citations for Roche :
501 - 550 of 1110 citations for Rnase 3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2019Quote: ... 1μL DTT (0.1 M) and 0,5 μL of protector rnase inhibitor (40 U/μL, Hoffmann-La Roche, Basel, Switzerland) were added and the mix incubated at 37° C per 2 min ...
-
bioRxiv - Neuroscience 2021Quote: ... 1x PBS; Invitrogen, 0.5% RNase free BSA;Serva, 1:200 RNaseIN plus inhibitor; Promega, 1x EDTA-free protease inhibitor; Roche) and filtered through 40 μm filter (BD falcon ...
-
bioRxiv - Neuroscience 2021Quote: ... the beads were resuspended in EMSA 1X with 100 U/ml RNAse inhibitor and 1X protease inhibitor cocktail (Roche). FRMP binding assay was performed by incubating biotinylated transcripts (250 ng ...
-
bioRxiv - Microbiology 2022Quote: ... cells were resuspended in a 50 mM sodium phosphate buffer at pH 7.8 (buffer A) supplemented with DNase I (100 U, DNase I recombinant, RNase-free Roche), and disrupted using a French Pressure Cell (3 cycles at 1100 psi) ...
-
bioRxiv - Microbiology 2021Quote: ... RNA samples were incubated for 30 min at 37°C with 20 U of RNase-free DNase I (Roche) in a final reaction volume of 30 μl ...
-
bioRxiv - Pathology 2022Quote: ... 2.5 μl of cDNA in RNase free water was made up to 25 μl with FastStart Universal SYBR Green Master (ROX, 12.5 μl, Roche), Ultra Pure Water (8 μl ...
-
bioRxiv - Developmental Biology 2022Quote: ... Precipitated RNA was resuspended in RNase- and DNase-free water and DNA digestion was performed using DNase I (Roche) with 2U/ul of RNasin® Plus (Promega ...
-
bioRxiv - Molecular Biology 2019Quote: ... Crosslinks were reversed at 65°C overnight and the eluates were treated with RNase A and Proteinase K (Roche), prior to an extraction with phenol:chloroform:isoamyl alcohol ...
-
bioRxiv - Immunology 2020Quote: DNase treatment was performed prior to Reverse transcription polymerase chain reaction (RT-PCR) using RNAse-free DNase I (Roche) at 37°C for 10 min ...
-
bioRxiv - Developmental Biology 2021Quote: ... samples were pooled by stage and underwent three rounds of centrifugation at 1500rcf at 4°C for 10 minutes followed by rehydration with DPBS with 0.5% non-acetylated BSA and 0.5U/μl RNAse inhibitor (Roche 3335399001).
-
bioRxiv - Developmental Biology 2020Quote: ... genomic DNA was digested on column with the addition of an extra 1μl of RNase-free DNase I (Roche) to ensure complete digestion ...
-
bioRxiv - Cell Biology 2021Quote: ... 1x PBS; Invitrogen, 0.5% RNase free BSA;Serva, 1:200 RNaseIN plus inhibitor; Promega, 1x EDTA-free protease inhibitor; Roche) and filtered through 40 μm filter (BD falcon ...
-
bioRxiv - Neuroscience 2020Quote: ... 1x PBS; Invitrogen, 0.5% RNase free BSA;Serva, 1:200 RNaseIN plus inhibitor; Promega, 1x EDTA-free protease inhibitor; Roche) and filtered through 40 μm filter (BD falcon ...
-
bioRxiv - Developmental Biology 2022Quote: ... samples from the same stage were pooled and resuspended in DPBS with 0.5% non-acetylated BSA and 0.5U/μl RNAse inhibitor (Roche 3335399001).
-
bioRxiv - Neuroscience 2023Quote: ... Supernatants were removed and pellets were re-suspended in 2% BSA/PBS containing RNase inhibitor (0.2 U/μL) (Roche). Nuclei were incubated with fluorescently conjugated antibodies against the neuronal marker NEUN (RBFOX3/NEUN (1B7 ...
-
bioRxiv - Genomics 2023Quote: ... Cells were centrifuged at 500 x g for 5 min at 4C and 500 uL 1X PBS + 1% BSA + DAPI + 0.5U/uL Protector RNAse Inhibitor (Roche) was added ...
-
bioRxiv - Immunology 2023Quote: ... The DNA samples were treated with 0.5 μg/μl of RNase A for 30 min at 37°C and then with 0.25 μg/μl of Proteinase K (Roche) in the presence of 10mM EDTA ...
-
bioRxiv - Molecular Biology 2023Quote: SDGC-SEC-purified stress granule cores (strain JD1370) were incubated at 0.23 A260 units/mL with 2 or 4 units of RNase H (10786357001; Roche) in 40 μL of RNase H buffer (20 mM HEPES-KOH pH 8.0 ...
-
bioRxiv - Immunology 2024Quote: ... The digestion was stopped by adding one volume of 2X STOP Buffer (340mM NaCl, 20mM EDTA, 4mM EGTA, 0.02% Digitonin, 50ug/mL RNase A (Cat #3335399001, Roche), 50ug/mL Linear Acrylamide (Cat #K548 ...
-
bioRxiv - Plant Biology 2024Quote: ... the tissue was finely chopped on ice for approximately 1 minute using 600 μL of pre-chilled Nuclei Isolation Buffer containing 0.4U/μL RNase inhibitor (Roche, Protector RNase Inhibitor ...
-
bioRxiv - Cancer Biology 2020Quote: ... An overnight blunt ligation was induced for the 5’ of exon 1 and the 3’ of exon 3 (5 U T4 DNA ligase (Roche, Basel, Switzerland)) at room temperature ...
-
bioRxiv - Developmental Biology 2020Quote: ... 5-Bromo-4-chloro-3-indolyl phosphate (BCIP, [Roche]) and nitro blue tetrazolium chloride (NBT ...
-
bioRxiv - Cell Biology 2021Quote: ... and 3× EDTA-free complete protease inhibitor cocktail (Roche). Lysates were briefly sonicated until the protein solution was clear ...
-
bioRxiv - Cancer Biology 2022Quote: ... incubated with blocking buffer containing 3 % BSA (Roche Diagnostics), 5 % goat serum (Jackson ImmunoResearch Laboratories) ...
-
bioRxiv - Neuroscience 2020Quote: Clonazepam (CAS #1622-61-3) was bought from Roche on 2018 ...
-
bioRxiv - Developmental Biology 2023Quote: ... 5-Bromo-4-chloro- 3-indolyl phosphate (BCIP, [Roche]) and nitro blue tetrazolium chloride (NBT ...
-
bioRxiv - Biochemistry 2023Quote: ... 3 cOmplete EDTA-free protease inhibitor Cocktail tablets (Roche), pH 7.2 ...
-
bioRxiv - Biophysics 2024Quote: ... 20 mM imidazole) supplemented with 3 protease inhibitors (Roche) and then lysed by an EmulsiFlex-C3 (Avestin ...
-
bioRxiv - Developmental Biology 2024Quote: ... 5-bromo-4-chloro-3-indolyl-phosphate (BCIP, Roche), and levamisole (1359302 ...
-
bioRxiv - Cancer Biology 2021Quote: ... + 0,4 g/l of human albumin (Vialebex) with Liberase TL (Roche) 150 ug/ml and DNase 1 (Sigma ...
-
bioRxiv - Biochemistry 2022Quote: Human Bax cDNA was cloned in a pIVEX 2.4 plasmid (Roche). The cloning was done so that the His6 tag was absent from the construction ...
-
bioRxiv - Genomics 2019Quote: ... using SeqCAP EZ Human Exome Library v3.0 (Roche NimbleGen, Madison, WI) for exome sequence capture ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 mL human insulin (final concentration 20 μg mL-1, Roche), 1ml BSA (final concentration 50 μg mL-1 ...
-
bioRxiv - Cancer Biology 2022Quote: ... and human epidermal growth factor 2 (HER-2) (790–4493, Roche).
-
bioRxiv - Immunology 2023Quote: ... and human rIL-2 (30 U/ml) (Roche/Merck, Darmstadt, Germany) to the culture ...
-
bioRxiv - Cell Biology 2023Quote: ... Sequencing was performed using the SeqCap EZ Human Exome Library (Roche) and a NovaSeq 6000 sequencer (Illumina) ...
-
bioRxiv - Molecular Biology 2024Quote: Human genomic DNA of high molecular weight (Roche Diagnostics, Basel, Switzerland) were processed in a single replicate per DNA extraction protocol (Table 1) ...
-
bioRxiv - Genomics 2019Quote: ... An additional Proteinase K incubation (65 μl of 10mg/mL per 7 million cells starting material) at 65°C for two hours was followed by RNase A (Roche; 15 μl of 10mg/mL per 7 million cells starting material ...
-
bioRxiv - Biochemistry 2020Quote: ... S2 cells were lysed in S2 lysis buffer (20mM Tris at pH7.4, 200mM KCl, 0.1% Tween-20,0.1% Igepal, EDTA-free Complete Protease Inhibitor Cocktail [Roche], 100μg/mL RNase A). Supernatant was cleared by centrifugation at 4,000 x g for 20 minutes at 4°C ...
-
bioRxiv - Biochemistry 2020Quote: ... cells were lysed in S2 lysis buffer (20mM Tris at pH7.4, 200mM KCl, 0.1% Tween-20,0.1% Igepal, EDTA-free Complete Protease Inhibitor Cocktail [Roche], 100μg/mL RNase A). Lysates were divided into four equal fractions ...
-
bioRxiv - Neuroscience 2021Quote: ... Coli tRNA at RT for 10 minutes and resuspended in EMSA 1X with 100 U/ml RNAse inhibitor and 1X protease inhibitor cocktail (Roche). The assay was then performed in presence of 250 ng of biotinylated transcript ...
-
bioRxiv - Cell Biology 2021Quote: ... 0.5% NP-40 and supplemented with RNase inhibitor (Murine, M0314, New England Biolabls) and protease inhibitors cocktail (cOmplete Mini, Roche), and passed through a 25G needle 7 times ...
-
bioRxiv - Immunology 2021Quote: ... 25-100 ng of total RNA was used to deplete rRNA using DNA probes and RNase H provided in RiboErase (HMR) kit (Roche). The depleted RNA was fragmented prior to cDNA synthesis followed by Illumina adaptor ligation using KAPA RNA HyperPrep kit (Roche) ...
-
bioRxiv - Pathology 2022Quote: ... 2 μl of cDNA in RNase free water was made up to 20 μl with FastStart Universal SYBR Green Master (ROX, 10 μl, Roche), Ultra Pure Water (6.4 μl ...
-
bioRxiv - Biophysics 2021Quote: RNase activity of RnlA and its mutants was monitored by following the cleavage of bacteriophage MS2 genomic RNA (Roche Diagnostics) via denaturing gel electrophoresis as described (28) ...
-
bioRxiv - Genomics 2021Quote: ... Chromatin cross-linking was reversed at 65°C for 5 hours with 280 mM NaCl and 88 μg/mL RNase DNase free (11119915001, Roche). Proteins were then digested using 88 μg/mL of Proteinase K (03115828001 ...
-
bioRxiv - Molecular Biology 2021Quote: ... An equal volume of lysis buffer and 110 U/ml of DNase (DNase I recombinant, RNase-free solution, Cat no. 4716728001, Roche) was added to the ground cell lysate followed by incubation for 1 hour at 4°C ...
-
bioRxiv - Cancer Biology 2022Quote: ... Genomic DNA contamination was removed by DNase I treatment of the isolated RNA using DNase I RNase free kit (Roche). After quantification of the RNA and quality check ...
-
bioRxiv - Neuroscience 2022Quote: ... Tissue was dounce homogenized in a nuclear extraction buffer (10mM Tris, 146mM NaCl, 1mM CaCl2, 21mM MgCl2, 0.1% NP-40, 40u/mL Protector RNAse inhibitor (Roche 3335399001). Dounce homogenizer was washed with 4mL of a washing buffer (10mM Tris ...
-
bioRxiv - Molecular Biology 2023Quote: ... treated with 1 µL RNase A (Thermo) for 30 minutes followed by 1 µL Proteinase K (20 mg/mL, Roche) for 1 hour at 37°C ...