Labshake search
Citations for Roche :
4751 - 4800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... on a LightCycler 480 II (Roche Life Sciences). Each qPCR run included a standard curve of 10-fold serial dilutions of a known concentration of R ...
-
bioRxiv - Microbiology 2024Quote: ... Signals were visualized by CDP-star (#11759051001, Roche) on a ChemScope imaging station and quantified using ImageJ Software.
-
bioRxiv - Microbiology 2024Quote: ... membrane was hybridized with 75 mU/ml Anti-Digoxigenin-AP (#11093274910, Roche) in blocking solution for 60 min at 37°C ...
-
bioRxiv - Immunology 2024Quote: ... and 125 ug/ml of DNAse I (Roche 11284932001) for 30’ and then mechanically disaggregated in gentleMACS® tissue dissociator.
-
bioRxiv - Immunology 2024Quote: ... with DNAse I 0,1mg/ml (Roche 11-284-932-001) and Liberase® 0,1 mg/ml (Roche 05-401-020-001).
-
bioRxiv - Immunology 2024Quote: ... sciatic nerves and brains were crushed with the back of a 3ml syringe in a serrated 24 well plate and triturated in digestion media (Liberase TM (0.208 WU/ml) (Roche Cat # 054010200001) and DNase I (40 ug/ml ...
-
bioRxiv - Immunology 2024Quote: Proteins were extracted from tissues or cells in radioimmunoprecipitation assay (RIPA) buffer (Beyotime) containing a complete protease inhibitor cocktail (Roche), resolved by SDSPAGE ...
-
bioRxiv - Immunology 2024Quote: ... with 0.17 U/ml of Liberase® (Roche 05-401-020-001), 1 mg/ml of collagenase (Sigma-Aldrich 11088866001 ...
-
bioRxiv - Immunology 2024Quote: ... 5% glycerol and 1x EDTA-free protease inhibitor cocktail (PIC) (Roche).
-
bioRxiv - Immunology 2024Quote: ... Libraries were then equimolarly pooled and quantified by qPCR using the KAPA library quantification kit (Roche). Sequencing was carried out on the NovaSeq 6000 instrument from Illumina using paired-end 2 × 100 bp ...
-
bioRxiv - Immunology 2024Quote: ... They were then quantified by qPCR using the KAPA Library Quantification Kit (Kapa Biosystems, Roche, Basel, Switzerland) before amplification with Illumina’s cBot ...
-
bioRxiv - Immunology 2024Quote: ... and 0.2 mg/mL DNaseI (Roche, 11284932001) for 30 minutes at 37°C while shaking at 180 rpm ...
-
bioRxiv - Immunology 2024Quote: ... and 0.4 mg/mL DNaseI (Roche, 11284932001), for 30 minutes at 37 °C with shaking (180 rpm) ...
-
bioRxiv - Immunology 2024Quote: ... The concentration of each library was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument ...
-
bioRxiv - Immunology 2024Quote: ... and then i5/i7 barcoded primers were added to tagmented cDNA using Illumina Nextera XT Index Kit v2 and KAPA HiFi HotStart ReadyMix (Roche) to barcode amplicons ...
-
bioRxiv - Immunology 2024Quote: ... The thymic pieces were then transferred to a digestion solution containing collagenase/dispose (Roche) at 1mg/ml and DNase I 20ng/ ml in 8ml of 2% FBS + PRMI1640 medium ...
-
bioRxiv - Immunology 2024Quote: Bacterial loads were evaluated by mechanical disruption of organs in 0.05% v/v Tween 20 in PBS containing a proteinase inhibitor cocktail (Roche) prepared according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... followed by staining of CD3 and PD-1 w with a BenchMark Ultra autostainer (Roche). Tim-3 was stained manually after heat-induced epitope retrieval using the “high pressure” program on an electric pressure cooker (Cuisinart CPC-600 ...
-
bioRxiv - Immunology 2024Quote: ... 500ng of total RNA was treated with DNAse (Roche), and retrotranscribed to cDNA using random hexamers (Roche ...
-
bioRxiv - Immunology 2024Quote: ... and 0.2 mg/mL DNaseI (Roche) for 1 hour at 37 °C with shaking (180 rpm) ...
-
bioRxiv - Immunology 2024Quote: ... and 0.2 mg/mL DNaseI (Roche) for 30 minutes at 37 °C while shaking at 400 rpm ...
-
bioRxiv - Immunology 2024Quote: ... and retrotranscribed to cDNA using random hexamers (Roche) and M-MLV reverse transcriptase (Promega) ...
-
bioRxiv - Immunology 2024Quote: ... The concentration of each library was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument ...
-
bioRxiv - Immunology 2024Quote: ... Libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase (HMR) (cat. 08098131702, Roche) and purified by AMPure SPRI beads (cat ...
-
bioRxiv - Immunology 2024Quote: ... DNAse I 250 µg/ml (Roche 11284932001), 2% FBS (Eurobio ...
-
bioRxiv - Immunology 2024Quote: ... and Liberase® 0,1 mg/ml (Roche 05-401-020-001).
-
bioRxiv - Immunology 2024Quote: ... FL fragments were treated for 20 minutes at 37°C with 100 μg/mL of Liberase (Roche) to obtain a cell suspension ...
-
bioRxiv - Immunology 2024Quote: ... The LightCycler® 480 Instrument II (Roche) was used for qPCR analysis.
-
bioRxiv - Immunology 2024Quote: ... Liberase® 100 µg/ml (Roche 05-401-020-001), DNAse I 250 µg/ml (Roche 11284932001) ...
-
bioRxiv - Immunology 2024Quote: ... and were kept in PBS containing 25mM EDTA and protease inhibitor (cOmplete tablets, Roche). The tissues were then lysed with a tissue homogenizer ...
-
bioRxiv - Immunology 2024Quote: ... antibody-derived oligomers were amplified and indexed by mixing 45 μL eluent with 50 μL of KAPA HiFi HotStart ReadyMix (KAPA Biosystems) and 5 μL of forward/reverse indexing primers (Biolegend) ...
-
bioRxiv - Immunology 2024Quote: ... in 20 µl of DOTAP (11 811 177 001, Roche Inc.). For the intranasal procedures ...
-
bioRxiv - Immunology 2024Quote: ... They were then quantified by qPCR using the KAPA Library Quantification Kit (Kapa Biosystems, Roche, Basel, Switzerland) before amplification with Illumina’s cBot ...
-
bioRxiv - Immunology 2024Quote: ... Reactions were run in triplicate on LightCycler® 480 Instrument II (Roche).
-
bioRxiv - Immunology 2024Quote: ... and 0.2 mg/mL DNase I (Roche, #11284932001) for 120 minutes at 37°C undergoing constant ...
-
bioRxiv - Immunology 2024Quote: ... whole pituitaries or separate posterior and anterior pituitaries were mechanically lysed by suspending and digested with 50 µg/ml DNase 1 (Roche) and 1 mg/ml Collagenase D (Roche ...
-
bioRxiv - Immunology 2024Quote: ... and 1 mg/ml Collagenase D (Roche) in Hank’s Buffered Saline (HBSS ...
-
bioRxiv - Immunology 2024Quote: ... Extracted RNA samples underwent library preparation for polyA tailed mRNA with a RNA concentration of >1ng/1ul using with Kapa RNA hyperPrep kit (Roche), followed by 75 cycles of Next-generation sequencing with NextSeq® (Illumina ...
-
bioRxiv - Immunology 2024Quote: ... and 100 micrograms per milliliter DNaseI (Roche) in RPMI-1640 (Gibco ...
-
bioRxiv - Immunology 2024Quote: ... Thawed synovial tissues were disaggregated into single-cell suspensions by mincing and digesting with 100 micrograms per milliliter LiberaseTL (Roche) and 100 micrograms per milliliter DNaseI (Roche ...
-
bioRxiv - Immunology 2024Quote: ... 1% Triton X-100) supplemented with cOmplete™ Protease Inhibitor Cocktail (#11836170, Roche). Lysates were then cleared by centrifugation for 15 minutes at 14,000 rpm at 4°C and subjected to immunoprecipitation with anti-V5 mouse monoclonal antibody (#MCA1360 ...
-
bioRxiv - Immunology 2024Quote: Lung tissue was prepared by coarsely chopping the tissue followed by incubation in a 0.5 mg/mL solution of collagenase D (Roche) and 500 Units of DNase (Sigma Aldrich ...
-
bioRxiv - Microbiology 2024Quote: ... 5′-32P-end-labeled deoxynucleotide primer were hybridized to complementary regions of rRNA (16S nucleotides 1494–1510) and extended with AMV reverse transcriptase (Roche). Extension products were run on denaturing polyacrylamide/urea gel and visualized by autoradiography using a Typhoon PhosphorImager (Amersham Bioscience ...
-
bioRxiv - Molecular Biology 2024Quote: ... 500 nM ADP-HPD (a PARG inhibitor; sigma) and 1× protease inhibitor cocktail (Roche)] and incubated for 30 min on ice with gentile vortexing and then centrifuge at maximum speed for 15 minutes at 4°C in microcentrifuge to remove the cell debris ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1× protease inhibitor cocktail (Roche), 10 μM PJ34 (Sigma) ...
-
bioRxiv - Microbiology 2024Quote: ... and 1 tablet per 50 ml complete EDTA-free protease inhibitors (Roche). The cells were subsequently disrupted using an Emulsiflex-C5 high-pressure homogenizer (Avestin ...
-
bioRxiv - Microbiology 2024Quote: ... and 10% glycerol) supplemented with the complete EDTA-free protease inhibitor cocktail (1 tablet/50 ml of lysate; Roche), frozen in liquid nitrogen ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1X cOmplete EDTA-free protease inhibitor cocktail (Roche), and 1X PhosSTOP phosphatase inhibitor (Roche ...
-
bioRxiv - Molecular Biology 2024Quote: ... Purified ATAC-seq libraries were assessed by Bioanalyzer and quantified by Qubit and KAPA Library Quantification (Roche). Paired-end sequencing (60 bp ...
-
Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2bioRxiv - Molecular Biology 2024Quote: ... cells were washed in PBS and lysed in 50 mL McIlvaine buffer containing EDTA-free cOmplete Mini Protease Inhibitors (Roche). Cells were shaken at 4°C and 450 rpm for 10 min followed by one hour of incubation at 4°C ...