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Citations for Roche :
4851 - 4900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... using a LightCycler480 (Roche) with primers ...
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bioRxiv - Microbiology 2024Quote: ... each sample was normalized to 3.5 ng in 5 µL water before generating libraries using a 1:10 Kapa HyperPlus (Roche) miniaturized protocol with barcoded indices as previously described82 ...
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bioRxiv - Microbiology 2024Quote: ... Broncholalveolar lavage (BAL) fluids were obtained by intratracheal injection of 2 × 1 ml PBS supplemented with protease inhibitors (Roche). Serum was collected by intracardiac puncture and separated from whole blood in Z-Gel microtubes (Sarstedt) ...
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bioRxiv - Microbiology 2024Quote: ... double-stranded complementary DNA (cDNA) was synthesized using the cDNA synthesis system (Roche, UK) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2024Quote: ... 10μl /well cell proliferation reagent WST-1 (Roche 05015944001) was added ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were prehybridized in DIG Easy Hyb (#11796895001, Roche) for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... by automated IHC stainer (Ventana BenchMark ULTRA, Roche Diagnostics, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and 0.5% n-octyl-β-D-glucopyranoside) supplemented with protease inhibitors (Roche), and ultrasonicated for protein extraction ...
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bioRxiv - Microbiology 2024Quote: ... the cells were lysed in 180 µl of TNN buffer (100 mM Tris-HCl, pH 8.0, 250 mM NaCl, 0.5% Nonidet P-40 and cOmplete protease inhibitor cocktail [Roche, Switzerland]) for 10 min on ice ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were resuspended in lysis buffer (50 mM HEPES, pH7.5, 100 mM KCl, 5% glycerol, 0.3% Triton X-100, 1X Roche EDTA-free Complete Protease Inhibitor cocktail ...
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bioRxiv - Molecular Biology 2024Quote: ... before being spotted in 2.5 mcl drops onto a dry nylon membrane (Hybond-N+, Roche 11417240001). Biotinylated oligo-dT (Promega Z5261 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1% IGPAL-CA-630) supplemented with protease and phosphatase inhibitors (Roche) and the lysates were cleared ...
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bioRxiv - Molecular Biology 2024Quote: ... Quantitative real-time PCR (qRT-PCR) was performed using MonAmp™ ChemoHS qPCR Mix (Monad, Suzhou, China) and LightCycler® 480 II (Roche Diagnostics, Germany). Table S2 contains the primer sequences ...
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bioRxiv - Microbiology 2024Quote: ... EDTA-free protease inhibitor cocktail (Roche). After high-speed centrifugation at 20,000 rpm for 2 h at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... 60 ul of Protein A agarose beads (Roche, PROTAA-RO) were added ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1X PhosSTOP phosphatase inhibitor (Roche) before centrifuging at 16,000 g for 10 min at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... 5% glycerol) supplemented with a cOmplete Protease Inhibitor Cocktail (Roche). Cells were lysed by sonication and the lysate was centrifuged (21 000 g ...
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bioRxiv - Molecular Biology 2024Quote: ... and resuspended in the same buffer supplemented with a cOmplete Protease Inhibitor Cocktail (Roche) and lysozyme (0.2 mg/mL) ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 mM Tris-HCl [pH 7.5], 1 mM DTT, 0.1% NP-40, and 1× protease inhibitor EDTA-free mixture [Roche]) on ice for 30 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR reactions were carried out on 2 µL of cDNA using 7 µL of FastStart SYBR Green Master Mix (Roche, Basel, Switzerland), 2 µL of ddH2O (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... 1× EDTA protease inhibitor cocktail (Roche), and 100 U/ml RNaseOUT (Life Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... Illumina-compatible sequencing libraries were prepared by using a modified version of KAPA HyperPrep library kit (KAPA BioSystems, Willmington, MA). Quality check steps were performed to assess the fraction of proximally ligated DNA labeled with biotin ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA (2 µl) was added to the mixture with FastStart SYBR Green Master Mix kit (Roche, Basel, Switzerland) and specific primers SP_F 5’-GAC-CAG-TCG-AAC-GCA-CAT-TG-3’ and SP_R 5’-CGG-AGA-GGG-TTG-TTG-TGT-CT-3’ ...
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bioRxiv - Molecular Biology 2024Quote: ... The Fast-Start SYBR Green Master Mix (Roche, Basel, Switzerland) was used according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 µL of ddH2O (Roche, Basel, Switzerland) and 0.5 µL (80 ng/µL ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.50 μl 10x PCR reaction buffer including 15 mM MgCl2 (Roche); 1.50 μl dNTPs mix (250 μM each dNTP) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 5 U of enzyme mix using the Expand Long Template PCR system (Roche). Samples were amplified with the following thermocycler conditions ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR reactions were carried out in 15 µl using the Taq DNA Polymerase kit (Roche) and the following conditions ...
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bioRxiv - Molecular Biology 2024Quote: Cells were lysed in RIPA buffer supplemented with 1x EDTA-free Protease Inhibitor cocktail (Roche), 1mM PMSF and 35 nM β-mercaptoethanol (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells intended for sucrose sedimentation were disrupted in standard lysis buffer with 1X protease inhibitor cocktail replaced by 1x complete mini EDTA-free protease inhibitor cocktail (Roche #04693159001). 1X Phosphatase Inhibitor (Sigma-Aldrich #P5726 ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR from genomic DNA was performed using KAPA2G Fast Hotstart Ready Mix PCR Kit (Kapa Biosystems). PCR products were cloned in pGEM-T Easy Vector (Promega) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1x complete mini EDTA-free protease inhibitor cocktail (Roche #04693159001). Cell extracts were clarified by centrifugation and were sedimented by ultracentrifugation for 120 min at 70,000 rpm (Beckman Optima Max-TL ...
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bioRxiv - Neuroscience 2024Quote: The following primary antibodies and reagents were used in this study: rat anti-HA (Roche, 3F10, 1/1000), rabbit anti-GFP (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Lobes were separated into clusters of 1-5 oocytes and the follicular layer was removed i) enzymatically with Liberase (Roche) and ii ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5% CA-630 and 10% glycerol) supplemented with protease inhibitor cocktail (Roche) on ice for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... and RNase A (Roche, Basel, Switzerland) at 37 °C for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 Unit of MNase (Roche, 10107921001) per million cells ...
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bioRxiv - Molecular Biology 2024Quote: ... Dicer was detected using anti-HA 3F10 monoclonal primary antibody (High Affinity rat IgG1, Roche #11867431001; dilution 1:500), anti-HA rabbit primary antibody (Cell Signaling ...
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bioRxiv - Microbiology 2024Quote: ... or HA (Cell signaling, 3724, 1:1,000; Cell signaling, 2999, 1:1,000; Roche, 54732500, 1:1,000). Secondary horseradish peroxidase (HRP)-conjugated antisera recognizing rabbit ...
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bioRxiv - Molecular Biology 2024Quote: ... qRT-PCR was performed using the KAPA SYBR FAST ABI Prism 2x qPCR Master Mix (Roche, Switzerland) and a QuantStudio 6 Flex Real-Time PCR system (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... were subjected to library preparation after rRNA depletion and poly(A)+ RNA enrichment using the Kapa Hyper Stranded mRNA library kit (Roche) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... containing EDTA-free cOmplete protease inhibitor mixture (Roche) and 15LmM imidazole ...
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bioRxiv - Microbiology 2024Quote: ... 500 μg cell lysate was diluted to 500 μL in lysis buffer supplemented with 1 mM DTT and 25 μg poly(dI:dC) (Roche) and precleared by incubating with 50 μL streptavidin beads for 1 hour at 4°C on rotating wheel ...
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bioRxiv - Microbiology 2024Quote: ... the Cell proliferation Kit II (XTT) (Roche, Basel, Switzerland) was used ...
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bioRxiv - Neuroscience 2024Quote: ... Protein extraction buffer (150 mM NaCl, 1% Triton x-100, 50 mM TrisHCl pH 7.4 with protease inhibitor (11836170001 Roche)) ...
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bioRxiv - Molecular Biology 2024Quote: ... and organoids were scraped or collected in ice-cold PBS containing Complete protein inhibitor cocktail tablets (Roche) (unless otherwise stated ...
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bioRxiv - Zoology 2024Quote: ... which were quality checked on an Agilent 4200 Tapestation and quantitated using a Lightcycler qPCR assay (Roche Life Science, St. Louis, MO). Libraries were paired-end sequenced (2 x 150 bp ...
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bioRxiv - Neuroscience 2024Quote: ... The minced tissues after the centrifuge were incubated with collagenase IV (2.5 mg/ml, ROCHE, Cat# 11088858001) at 37 °C with gentle rotation for 30 min in 2 ml of HBSS++ ...
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bioRxiv - Cell Biology 2024Quote: ... and 5 μg/ml phytohemagglutinin (Roche) to generate T cell blasts (34).
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bioRxiv - Plant Biology 2024Quote: ... 1 mM PMSF and 1 x complete protease inhibitor cocktail (Roche). Immunoprecipitation experiments with GFP-trap beads were performed according to the manufacturer’s protocol ...