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Citations for Roche :
4801 - 4850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... 10 µM MG132) containing the EDTA-free protease inhibitor cocktail (Roche). After 15 min of incubation at 4°C ...
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bioRxiv - Genetics 2024Quote: ... and qPCR was carried out using the FastStart Essential DNA Green Master (Roche) in a CFX Connect Real-Time System (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... and 1x KAPA Hifi Hotstart ReadyMix (KAPA Biosystems, Wilmington, MA)] ...
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bioRxiv - Systems Biology 2024Quote: ... Cholesterol levels were quantified using an enzymatic assay (Cobas, Roche) according to the manufacturer’s protocol.
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bioRxiv - Systems Biology 2024Quote: ... 1 ng was taken as a template for a PCR reaction (Supplementary Table 4, primers F: 42-65, R: 66-77) using KAPA HiFi HotStart ReadyMix (Roche, KK2602) for adding IIlumina indices and machine adapters ...
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bioRxiv - Synthetic Biology 2024Quote: ... and then purified by a Biorad NGC using a IMAC column (Roche). The crude extracts are prepared as followed ...
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bioRxiv - Synthetic Biology 2024Quote: ... The Pgi and Zwf enzymes were obtained commercially from Roche. For Hps/ Phi Assay ...
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bioRxiv - Systems Biology 2024Quote: ... for each sample 350µl of type 1 rat-tail collagen (Roche) dissolved in acetic acid 0.2%v /v was supplemented with 15µl of sterile dd H2O ...
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bioRxiv - Neuroscience 2024Quote: ... Libraries were quantified using the KAPA Library Quantification Kits - Complete kit (Universal) (Kapa Biosystems). The average size fragment was determined using a LabChip GXII (PerkinElmer ...
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bioRxiv - Molecular Biology 2024Quote: ... Alkaline phosphatase staining was conducted on primary OP6 cell cultures on Day-5 post-transfection using NBT/BCIP stock solution (Roche/Sigma 11681451001) or BCIP/NBT-Blue Liquid Substrate Systems for Membranes (Sigma B3804 ...
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bioRxiv - Neuroscience 2024Quote: ... and 50mM HEPES (pH 7.4) supplemented with protease inhibitors (Roche) for 45 minutes at 4°C while gently shaking ...
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bioRxiv - Developmental Biology 2024Quote: ... and 5μL SYBR Green (Roche, Switzerland) per well ...
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bioRxiv - Evolutionary Biology 2024Quote: ... All the libraries were double-indexed using KAPA HiFi HotStart Uracil+ReadyMix (Roche). The resulting indexed libraries were quantified on a QubitTM dsDNA HS (Invitrogen ...
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bioRxiv - Physiology 2024Quote: ... Minas Gerais) in an automated biochemical analysis equipment (Cobas Mira S, Roche®, Basel, Switzerland).
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bioRxiv - Physiology 2024Quote: RNA-seq library preps were generated using the Kapa stranded mRNA-seq kit (Roche, Basel, Switzerland). Samples were individually barcoded sequenced on the Illumina Hi-Seq 4000 (Illumina Inc ...
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bioRxiv - Synthetic Biology 2024Quote: ... first using MASC PCR (Supplementary Data 1) in KAPA2G Fast Multiplex MasterMix (Roche, USA) and then by validating its sequence using Illumina whole genome sequencing ...
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bioRxiv - Physiology 2024Quote: ... 1x Protease inhibitor cocktail (Roche, #04693116001)) and homogenized by using Precellys 24 device and tubes (Precellys ...
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bioRxiv - Synthetic Biology 2024Quote: ... The final indexed libraries were quantified using KAPA Library Quantification Kit (Roche 7960140001) for pooling and sequenced at 2x150 cycles on NovaSeq X Plus by Novogene.
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bioRxiv - Molecular Biology 2024Quote: ... and 1% NP-40) supplemented with 1mM PMSF and protease cocktail inhibitor (Roche, cat no# 4693159001). A Rapid Gold BCA Protein Assay kit (Pierce ...
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bioRxiv - Synthetic Biology 2024Quote: PCR preparation of samples for sequencing was conducted using KAPA HiFi (Roche KK2101) for a total of 24 cycles in a two-step PCR ...
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bioRxiv - Synthetic Biology 2024Quote: PCR was performed using KAPA HiFi (Roche KK2101) for all experiments ...
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bioRxiv - Synthetic Biology 2024Quote: ... the clarified cell lysates were digested with micrococcal nuclease from Staphylococcus aureus (MNase, Roche, USA) for 1 hour at 25 °C under continuous agitation ...
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bioRxiv - Synthetic Biology 2024Quote: ... plasmids to be barcoded were diluted to 2 ng/µl and PCR amplified using the primers containing the full barcode sequence using KAPA HiFi HotStart ReadyMix (KK2602, Roche, USA). Barcoded PCR products were run on a 1.2% agarose gel ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability assays were conducted using WST-1 reagent (Roche). Tissue wares were purchased from BioStar ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1% phosphatase inhibitor (Roche, 4906837001). Lysates were centrifuged at 12,000 rpm at 4 °C for 10 min and the supernatant fraction was retained ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was quantified and poly(A) selected RNA-seq libraries were generated using a KAPA Stranded mRNA-seq kit (KAPA Biosystems) with 500 ng RNA per sample as starting material ...
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bioRxiv - Cancer Biology 2024Quote: ... pH 8.5) containing 5 μL proteinase K (18.7 mg/ml, Roche) and incubated for 30 min at 65 °C followed by the heat-inactivation of proteinase K at 95 °C 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... 100 mM TRIS (Fisher BioReagents™) pH 8.5 supplemented with PhosStop phosphatase inhibitor as well as cOmplete™ mini protease inhibitor cocktail (Roche). Samples were subjected to probe-based sonication using a Thermo Sonic Dismembrator and subsequently heated at 95° C for 10 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Sodium Deoxycholate 0.1%) containing protease inhibitors (Roche). Protein was quantified using Bradford assay (Protein Assay Kit ...
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bioRxiv - Cancer Biology 2024Quote: ... On the following day 10 µl MTT labeling reagent (5 mg/ml) of the Cell Proliferation Kit I (Roche) was added and cells were incubated at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... and Proteinase K (194 μg/ml, recombinant, PCR grade, Roche) for 2 hours at 56 °C to achieve cell lysis and protein digestion ...
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bioRxiv - Cancer Biology 2024Quote: ... 2% SDS) supplemented with protease inhibitors (cOmplete, EDTA-free Protease Inhibitor Cocktail, Roche) and phosphatase inhibitors (100 μM Na3VO4 CAS:13721-39-6 ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting polyA RNA was used as input into the KAPA RNA Hyper prep RNAseq library prep kit (Roche). Libraries were prepared with dual-index adapters ...
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bioRxiv - Cancer Biology 2024Quote: ... Relative gene expression was determined using the Quanta SYBR Green Master Mix Kit (Quanta Bio) on a LightCycler 96 (Roche Diagnostics), using GAPDH as the reference gene.
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bioRxiv - Cancer Biology 2024Quote: ... slides were incubated with primary anti-BrdU antibody (Roche,1117037600, 1:100) for 1 hour followed by the incubation with an anti-mouse biotinylated antibody (Vector Labs ...
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bioRxiv - Systems Biology 2024Quote: ... cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)) ...
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bioRxiv - Physiology 2024Quote: ... and insulin (Cobas 6000 immunochemical analyzer, Roche, Germany) to estimate HOMA IR ...
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bioRxiv - Cancer Biology 2024Quote: Whole cell lysates were collected using RIPA lysis buffer (Beyotime Biotechnology, Jiangsu, China) containing 1% protease inhibitor (Roche, 11697498001) and 1% phosphatase inhibitor (Roche ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Reverse transcription into cDNA was performed using the Transcriptor Reverse Transcriptase kit (Roche, Indianapolis, IN) and random hexamer primers (Roche ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Quantitative PCR (qPCR) was performed on a LightCycler 480 System (Roche) using LightCycler 480 SYBR Green I Master Mix reagents (Roche) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Crosslinked cell pellets were lysed in 5 mL cold lysis buffer (10 mM Tris-HCl, pH7.5; 10 mM NaCl; 5 mM MgCl; 0.1 mM EGTA; 1x Roche Complete protease inhibitor cocktail). Lysis was allowed to proceed for minutes on ice with intermittent gentle pipetting to obtain homogeneous suspensions of nuclei ...
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bioRxiv - Cancer Biology 2024Quote: ... 150 mM NaCl) and protease inhibitors (Roche, Penzberg, Germany). After 20 min of incubation on ice lysates were centrifuged at 14,000 rpm for 20 min at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... protein samples were subjected to 10% SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF) membranes (Roche, Mannheim, Germany). Afterwards ...
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bioRxiv - Neuroscience 2024Quote: ... Amplification and quantification were performed using a LightCycler® 480 instrument (Roche, Basel, Switzerland). The cycling program included a two-minute heat activation step at 95 °C ...
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bioRxiv - Immunology 2024Quote: ... EDTA-free protease inhibitor cocktail (Roche), and phosphatase inhibitor cocktail solution I (Wako)) ...
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bioRxiv - Microbiology 2024Quote: ... and a LightCycler 96 (Roche) for the detection ...
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bioRxiv - Microbiology 2024Quote: ... 0.5% NP-40) containing protease and phosphatase inhibitors (cOmplete and PhosStop, Roche), and processed as previously described (PMID ...
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bioRxiv - Genetics 2024Quote: ... and one PhosSTOP tablet (Roche)) was added to the samples on ice ...
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bioRxiv - Neuroscience 2024Quote: ... Accurate quantification of the final libraries for sequencing applications was determined using the qPCR-based KAPA Biosystems Library Quantification kit (Kapa Biosystems, Inc.). Each library was diluted to a final concentration of 1.4 nM and pooled equimolar prior to clustering ...
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bioRxiv - Molecular Biology 2024Quote: ... or SeqCap EZ MedExome Enrichment Kit (Roche) and sequenced on a HiSeq 4000 or HiSeq 2500 platform (Illumina) ...