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Citations for Roche :
4551 - 4600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... Quantitative real-time PCR (qPCR) was performed using the KAPATM SYBR FAST qPCR Kit (KAPA Biosystems) and StepOnePlusTM Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Genetics 2024Quote: ... 0.1% sodium deoxicholate) supplemented with 1x Complete Protease Inhibitor Cocktail (Roche) and 1 mM PMSF ...
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bioRxiv - Immunology 2024Quote: ... 1% Triton X-100) supplemented with cOmplete Protease Inhibitor Cocktail (Roche) and PhosSTOP (Roche) ...
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bioRxiv - Genetics 2024Quote: ... supplemented with cOmplete Protease Inhibitor Cocktail (Roche). The solution was transferred onto ice for incubation for 5 minutes and then centrifuged at full speed for 10 minutes at 4 °C ...
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bioRxiv - Genetics 2024Quote: ... RNA expression was visualized using alkaline phosphatase-conjugated anti-DIG antibody (Roche), using CBIP and NBT as substrates (Roche) ...
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bioRxiv - Genetics 2024Quote: ... 1.5 mM KCl and 1x EDTA-free protease inhibitor cocktail (Roche)) ...
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bioRxiv - Genetics 2024Quote: ... using CBIP and NBT as substrates (Roche). Each set of experiments was carried out in parallel to minimize experimental variation ...
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bioRxiv - Genetics 2024Quote: ... 1.6μl of dNTP (10mM each) (Roche; 4638956001), 1.6μl of 20μM Preamp primer (5’-[Btn]TGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNN*N ...
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bioRxiv - Genetics 2024Quote: Reagents consisted of TaqMan™ Gene Expression Master Mix (Roche Diagnostics), TaqMan control probes for Rn18s (Roche Diagnostics ...
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bioRxiv - Genetics 2024Quote: ... PCR pre-amplification was performed by adding 1X KAPA HiFi HotStart Ready Mix (KAPA Biosystems, KK2602), 0.1μM ISPCR primers (5’-AAGCAGTGGTATCAACGCAGAGT ...
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bioRxiv - Genetics 2024Quote: ... KAPA HiFi HotStart PCR Kit (Roche; KK2502) was used for indexing-amplification with NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Genomics 2024Quote: ... which were labelled by fluorochrome Cy3-dUTP (Jenna Bioscience, Jena, Germany), Fluorescein-12-dUTP (Jenna Bioscience, Jena, Germany) or biotin-16-dUTP (Hoffmann-La Roche, Basilej, Switzerland) using the nick translation method ...
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bioRxiv - Genetics 2024Quote: ... and developed with CSPD (Roche) at 37°C for 15 min ...
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bioRxiv - Genomics 2024Quote: ... were digested for 1 hour at 37°C under 110 rpm shaking conditions in DMEM supplemented with 2 mg/mL collagenase D (Roche) and 0.4 mg/mL Dispase (Gibco) ...
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bioRxiv - Genetics 2024Quote: ... and a Roche LightCycler 96 machine (Roche). The zebrafish βactin and GAPDH genes were used as housekeeping controls (βactin forward 5’-CGAGCTGTCTTCCCATCCA-3’ ...
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bioRxiv - Genetics 2024Quote: ... Digoxigenin (DIG)-labeled in-situ probes were synthesized using DIG RNA Labeling Kits (Roche, 11277073910). RNA in-situ hybridization was carried out as previously described (Thisse and Thisse ...
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bioRxiv - Genetics 2024Quote: ... Quantivative reverse transcription PCR (qPCR) reaction was performed using KAPA SYBR FAST qPCR Master Mix (Roche) and gene-specific primers (Table S2 ...
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bioRxiv - Genomics 2024Quote: ... 5% Triton X-100) supplemented with proteinase inhibitors (Roche Cat #118733580001). Chromatin was sheared to 100-300bp fragments on a Bioruptor pico sonicator (Diagenode ...
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bioRxiv - Genetics 2024Quote: ... libraries were quantified with the KAPA library quantification kit (Roche #07960298001). Libraries were sequenced on Illumina NextSeq 500 sequencer (75-bp paired-end reads ...
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bioRxiv - Microbiology 2024Quote: ... with additional 10 μM oseltamivir carboxylate (Roche) and 10 μM zanamivir (GSK ...
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bioRxiv - Immunology 2024Quote: ... Stranded mRNA libraries were prepared a KAPA mRNA HyperPrep Kit (Roche) and sequenced on the Illumina HiSeq 4000 platform (75bp single-end sequencing).
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bioRxiv - Cancer Biology 2024Quote: ... and rat anti-HA antibody (11867431001) was from Roche. Cycloheximide and lysosome inhibitor chloroquine were from Sigma-Aldrich ...
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bioRxiv - Genetics 2024Quote: ... and the RNA was precipitated with isopropanol using glycogen (Roche) as carrier ...
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bioRxiv - Immunology 2024Quote: ... All libraries were quantified via Agilent 2100 hsDNA Bioanalyzer and KAPA library quantification kit (Roche, Cat. #0797014001). Libraries were sequenced at a targeted depth of 25,000 reads per cell using the NovaSeq 6000 S2 100 cycle kit (Illumina).
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bioRxiv - Immunology 2024Quote: ... 0.2mg/mL Collagenase P (Roche), and 0.1mg/mL DNase I (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: Lymph nodes were mechanically disrupted with tweezers and incubated in RPMI with 0.8mg/mL Dispase II (Roche), 0.2mg/mL Collagenase P (Roche) ...
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bioRxiv - Immunology 2024Quote: ... the DNA libraries were prepared using the KAPA HyperPrep Kit (Roche, Basel, Switzerland) following the manufacturer’s instructions and sequenced on the Illumina NovaSeq platform with a 150 bp paired-end strategy ...
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bioRxiv - Immunology 2024Quote: ... 1 μg of total RNA was reverse transcribed using the Roche Transcriptor First Strand cDNA synthesis kit (Roche Life Sciences) according to manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... Tagmented DNA was amplified with Kapa Hifi Hot Start Kit (Kapa Biosystems) using 9 PCR cycles ...
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bioRxiv - Immunology 2024Quote: ... and 0.5 mg/mL collagenase P (ROCHE). Each skullcap was placed into a small tissue-culture dish (FALCON ...
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bioRxiv - Genetics 2024Quote: ... The dried pellets were resuspended in 200 µl of lysis buffer (50 mM Tris-HCl pH 7.5, 1 mM EDTA, 2.75 mM DTT, 1.1 mM PMSF, Roche protease inhibitor at 2x working concentration (Roche 04693132001)) ...
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bioRxiv - Genetics 2024Quote: ... Roche protease inhibitor at 2x working concentration (Roche 04693132001)) ...
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bioRxiv - Immunology 2024Quote: ... and quantified using the Kapa Library Quantification Kit (Kapa Biosystems). Each library was sequenced in one lane of HiSeq4000 (Illumina ...
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bioRxiv - Immunology 2024Quote: ... and protease and phosphatase inhibitors (Roche)) at 20 million T cells/mL at 4 °C ...
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bioRxiv - Genetics 2024Quote: ... along with a single -RT sample for each corresponding biological replicate using 2 μl cDNA and a no template control (NTC) with the LightCycler® 480 SYBR Green I Master kit (Roche). Ct values were measured with the Bio-Rad C1000 CFX96 Real-Time System and Bio-Rad CFX Software (qPCR primers ...
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bioRxiv - Genetics 2024Quote: ... RNA sequencing libraries were prepared using the KAPA mRNA HyperPrep Kit V2 (Roche). Paired-end 100x100 bp sequencing was performed on a NovaSeq 6000 (Illumina) ...
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bioRxiv - Genetics 2024Quote: ... genomic DNA was extracted from mouse tail clippings and PCR amplified using the KAPA Mouse Genotyping Kit (Roche) following manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... using LightCycler 480 Instrument II (Roche). mRNA levels for each experimental group were quantified using the comparative CT method.
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bioRxiv - Developmental Biology 2024Quote: ... Kidneys were incubated in alkaline phosphatase-conjugated anti-DIG antibody (1:4000, Roche, Cat#11093274910). For in situ detection ...
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bioRxiv - Developmental Biology 2024Quote: ... and 1mg/ml Papain (Roche, 10108014001) for 10 minutes at 37°C with trituration every 2 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... TMR red (Roche, 12156792910) was performed on e14.5 paraffin sections ...
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bioRxiv - Developmental Biology 2024Quote: ... Digoxigenin labeled antisense riboprobes were synthesized by in vitro transcription using a DIG RNA labeling kit (Roche, Cat#11277073910), followed by purification using Micro Bio-Spin P-30 gel columns (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked in MBST + 10% HISS + 2% BMB for 2 h at room temperature and incubated in alkaline phosphatase-conjugated anti-DIG antibody (1:4000, Roche, Cat#11093274910) diluted in blocking buffer until signal development ...
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bioRxiv - Microbiology 2024Quote: ... The reaction mix used was FastStart Essential DNA Green Master mix (Roche; 06402712001). Real time PCR assay was performed on Quant studio 5 real time PCR machine (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: PCLS (4mm diameter) were incubated (37°C, 5% CO2) with 10µL of WST-1 reagent (Roche) in 100µL of culture media for 1 hour ...
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bioRxiv - Immunology 2024Quote: ... and lung lobes were finely minced and incubated in 15 mL of complete media containing 1 mg/mL collagenase (Roche) and 17 U/mL DNase I (Sigma Aldrich ...
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bioRxiv - Immunology 2024Quote: ... 500uL of Ni+2-NTA beads (Roche, #5893682001) were packed onto a 12mL column and equilibrated into six packed bead volumes of equilibration buffer (50mM Tris ...
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bioRxiv - Immunology 2024Quote: ... and feed buffer were reconstituted using the supplied reconstitution buffer supplemented with an EDTA-free protease inhibitor tablet (Roche, #5892791001). The individual components were combined ...
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bioRxiv - Zoology 2024Quote: ... Animals were then incubated with anti-Digoxigenin-POD (1:1500 dilution; Roche, 11633716001) in a blocking buffer overnight ...
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bioRxiv - Neuroscience 2024Quote: ... 12 mM MgCl2 and 150 mM KCl in RNAse free water; 0.5 mM DTT, 1 µL/mL Protease Inhibitors Cocktail [Roche] ...