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Citations for Roche :
4651 - 4700 of 5245 citations for Human Cholecystokinin 12 CCK12 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: The full-length hAce2 gene was PCR-amplified from Mammalian Gene Collection cDNA clone (clone number MGC:47598) using a Kapa HiFi PCR kit (Kapa Biosystems) with a primer pair (Forward ...
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bioRxiv - Microbiology 2021Quote: ... the SARS-CoV-2 Spike gene with a 54-nucleotide deletion at the C-terminus was PCR-amplified from synthetic DNA (provided by Alex Ma, Academia Sinica, Taiwan) using the Kapa HiFi PCR kit (Kapa Biosystems) with a primer pair (Forward ...
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bioRxiv - Microbiology 2021Quote: ... Ten nanograms of cDNA were used as a template in a 5- l reaction mixture from a KAPA SYBR FAST qPCR kit (Kapa Biosystems). Primers used are listed in table S3 ...
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bioRxiv - Genetics 2020Quote: Genomic DNA was isolated from well-developed leaves of 14-day plants according to the modified CTAB procedure (Murray and Thompson, 1980) or by using the KAPA3G Plant PCR Kit (Kapa Biosystems). The PCR for genomic DNA isolated by CTAB was carried out in a 25 ml reaction mixture using Platinum Taq DNA Polymerase (Invitrogen ...
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bioRxiv - Genomics 2019Quote: All libraries were amplified off the array using the primers indicated in Supplemental Table 10 with KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems) as described above ...
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bioRxiv - Genetics 2020Quote: ... cDNA was synthesised from the total RNA with anchored-oligo(dT)18 primer and random hexamer primer using Transcriptor First Strand cDNA Synthesis Kit (Roche, Germany). One μg of RNA was added to each 20 μl RT reaction ...
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bioRxiv - Developmental Biology 2020Quote: PCR was performed on genomic DNA extracted from cell pellets or mouse tails to determine the loss of the Y chromosomal genes Uba1y and Ssty1 by utilizing KAPA Mouse Genotyping Kit (Roche, KK7302). Specific procedures were followed according to the reagent instructions ...
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bioRxiv - Genomics 2021Quote: ... The Illumina sequencing libraries were prepared by the Genomic Technology Core (Institute of Plant and Microbial Biology, Academia Sinica) using KAPA LTP Library Preparation Kit (KK8232; Roche, Switzerland) and KAPA Dual-Indexed Adapter Kit (KK8722 ...
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bioRxiv - Genomics 2021Quote: ... Strand-specific Illumina cDNA libraries were prepared using the KAPA Stranded RNA-Seq library preparation kit with 10 cycles of PCR (KAPA Biosystems). Library quality was assessed by BioAnalyzer (Agilent ...
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bioRxiv - Neuroscience 2019Quote: ... Automated purification of genomic DNA was conducted using a MagNA Pure 96 machine and commercial extraction kits (Roche Diagnostics, Mannheim, Germany). Five common OXTR SNPs were chosen based on previous studies suggesting associations with social behavior (OXTR rs237887 ...
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bioRxiv - Molecular Biology 2019Quote: ... and an equimolecular pool of libraries was prepared and titrated by qRT-PCR using the “Kapa-SYBR FAST qPCR kit forLightCycler480” (Kapa BioSystems, Fritz Hoffmann-La Roche ...
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bioRxiv - Developmental Biology 2019Quote: ... with on-column DNase digestion followed by cDNA synthesis using the Transcriptor First Strand cDNA synthesis kit (04379012001, Roche Molecular Diagnostics) using random primers ...
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bioRxiv - Microbiology 2019Quote: Viral nucleic acid was extracted from 200µl of the filtrate using the High Pure viral nucleic acid kit (Roche Diagnostics, USA) following the standard protocol ...
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bioRxiv - Plant Biology 2019Quote: ... The probes were labeled with digoxigenin (DIG) according to the manufacturer’s protocol (DIG High Prime DNA Labeling and Detection Starter Kit II, Roche, Basel, Switzerland). Northern blot procedures were performed as previously described (Jiang et al. ...
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bioRxiv - Plant Biology 2019Quote: ... were used as templates to synthesis DIG-labeled probes according to the manufacturer’s protocol (DIG High Prime DNA Labeling and Detection Starter Kit II, Roche, Basel, Switzerland). ULTRAhyb®-Oligo Hybridization Buffer (Ambion ...
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bioRxiv - Developmental Biology 2019Quote: ... The indexed DNA libraries were quantitated using qPCR in a Roche LightCycler 480 with the Kapa Biosystems kit for library quantitation (Kapa Biosystems) before cluster generation ...
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bioRxiv - Neuroscience 2019Quote: ... Libraries were prepared by the Genome Technologies core facility at The Jackson Laboratory using the KAPA mRNA HyperPrep Kit (KAPA Biosystems), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: An automated dual-indexed library was constructed with 250 ng of genomic DNA utilizing the KAPA HTP Library Kit (KAPA Biosystems) on the SciClone NGS instrument (Perkin Elmer ...
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bioRxiv - Microbiology 2019Quote: ... The pooled library was quality-checked by Fragment Analyzer (Advanced Analytical) and quantified with the KAPA Library Quantification Kit for ABI Prism (Kapa Biosystems). Sequencing was conducted in a 1×100 run on a HiSeq 4000 at the University of Oregon Genomics and Cell Characterization Core Facility (Eugene ...
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bioRxiv - Immunology 2019Quote: ... The quantity and quality of each pool was assessed using a Fragment Analyser and by qPCR using an Illumina Library Quantification Kit (KAPA Biosystems) on a Light Cycler LC480II (Roche) ...
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bioRxiv - Microbiology 2019Quote: ... All q-PCRs were performed in Rotor-Gene 6000 (Corbett Life Science) and using FastStart Essential DNA Green Master Kit (Roche, Switzerland). The total volume for qPCR was 10 μl ...
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bioRxiv - Genomics 2021Quote: ... RNA sequencing libraries were prepared on a Beckman Coulter Biomek i7 liquid handling platform using Roche Kapa mRNA HyperPrep strand specific sample preparation kits (Roche 08098123702) from 200 ng of purified total RNA according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... a paired-end library with a target insert size of 550-bp was prepared using KAPA LTP Library Preparation Kit (KK8232; Roche, Switzerland) without amplification ...
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bioRxiv - Genetics 2020Quote: ... Amplicons were pooled to 10 nM in EB buffer and the final concentration was determined using the Illumina Universal qPCR Amplification kit from Kapa Biosystems. All pooled samples were diluted to 4 nM ...
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bioRxiv - Genomics 2020Quote: ... Libraries were assessed using Bioanalyzer DNA High Sensitivity chips and quantified by quantitative PCR using Kapa Library Quantification Illumina/ABI Prism Kit protocol (KAPA Biosystems). Validated libraries were pooled in equimolar quantities and paired-end sequenced on an Illumina HiSeq X platform following Illumina’s recommended protocol to generate paired-end reads of 150 bases in length.
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bioRxiv - Evolutionary Biology 2021Quote: ... The fragments were end-repaired, A-tailed and ligated to Illumina compatible adapters (IDT, Inc) using a KAPA-Illumina library creation kit (KAPA biosystems). For CLR libraries ...
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bioRxiv - Cell Biology 2020Quote: ... Library size distribution was checked on an Agilent high-sensitivity DNA chip and the concentration of the indexed library was determined using the KAPA library quantification kit (KAPA Biosystems) according to the manufacturer’s instructions on the StepOnePlus system (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA libraries were quantitated using qPCR in a Roche LightCycler 480 with the Kapa Biosystems kit (Kapa Biosystems, Woburn, Massachusetts) prior to cluster generation ...
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bioRxiv - Genomics 2021Quote: ... RNA sequencing libraries were prepared on a Beckman Coulter Biomek i7 liquid handling platform using Roche Kapa mRNA HyperPrep strand specific sample preparation kits (Roche 08098123702) from 200 ng of purified total RNA according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... cDNA libraries were prepared from poly(A)-captured mRNA using a KAPA RNA HyperPrep Kit (Roche, Basel, Switzerland, Cat No. KK8541) with Truseq adapters ...
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bioRxiv - Neuroscience 2021Quote: ... 10 ng of cDNA were used for qPCR with the KAPA SYBR FAST qPCR Kit (KK4604, KAPA Biosystems, Wilmington, MA, USA) and the experiment run on the Biorad CFX96 system using the TqPCR protocol described in Zhang et al ...
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bioRxiv - Microbiology 2021Quote: ... The fragments were treated with end repair, A-tailing, and ligation of Illumina-compatible adapters (IDT, Inc) using the KAPA Illumina Library prep kit (KAPA biosystems). Libraries for the rest of the samples were prepared in 96-well plates ...
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bioRxiv - Microbiology 2020Quote: ... were amplified using primers Sb.1 - Sb.2 and Sb.3 to Sb.8 (Table 2) and labeled with digoxigenin (DIG) using the PCR DIG probe synthesis kit (Roche Diagnostics). Membranes were hybridized with these probes at 65°C and then washed at 68°C with decreasing concentrations (from 2X to 0.2X ...
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bioRxiv - Biochemistry 2021Quote: ... the amino acid Thr at position 206 was mutated to Trp using KAPA HiFi HotStart ReadyMix PCR Kit (Kapa Biosystems, USA) together with the designed complementary primers (5’- CCTATCTGATTCATGAGCACATGGTTATTTGGGATCGCATTGAAAAC-3’ and 5’- GTTTTCAATGCGATCCCAAATAACCATGTGCTCATGAATCAGATAGG-3’ ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then hybridized overnight at 70 °C with a digoxigenin (DIG)-labeled RNA probes (DIG RNA labeling kit, Roche 11277073910) for Gadd45b mRNA ...
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bioRxiv - Neuroscience 2020Quote: ... Titration of the viral vectors was performed by qPCR using a KAPA SYBR® FAST qPCR Master Mix Kit (Kapa Biosystems). The titre was 7.9 × 1013 GC/ml for AAV9/GFP ...
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bioRxiv - Developmental Biology 2021Quote: ... The libraries were quantified by fluorometry on a Qubit instrument (LifeTechnologies) and by qPCR with a Kapa Library Quant kit (Kapa Biosystems) prior to sequencing ...
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bioRxiv - Cancer Biology 2020Quote: ... mRNA sequencing libraries were generated from up to 250ng of total RNA and were prepared using a KAPA RNA HyperPrep kit (Roche Diagnostics) with polyA selection and indexed using a KAPA Dual-Indexed adapter kit (Roche Diagnostics) ...
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bioRxiv - Cancer Biology 2020Quote: Cell proliferation was measured by determining the extent of 5-Bromo-2’-deoxy-uridine (BrdU) incorporation into DNA of U87-MG cells using the BrdU cell proliferation assay ELISA kit (Roche, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... Libraries were then quantified through real-time PCR with the KAPA Library Quant Kit for Illumina (KAPA Biosystems catalog no. KK4835). Finally ...
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bioRxiv - Microbiology 2021Quote: ... primers and probe specific for SARS-CoV-2 RdRP (TibMolBiol; #53-0777-96) and a LightCycler Multiplex RNA Virus master kit (Roche; #07083173001) according to the instructions of the manufacturer ...
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bioRxiv - Genomics 2020Quote: ... The six STARR-Seq RNA replicate libraries and a STARR-Seq Plasmid DNA input library (amplified as before but with 20 ng DNA input and 15 PCR cycles) were normalized with the KAPA Library Kit (KAPA Biosystems) and sequenced on an Illumina NextSeq with 150-bp paired-end reads using standard protocols.
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bioRxiv - Molecular Biology 2021Quote: Random primed DNA probes were generated by using the Random Primed DNA Labeling Kit from Roche (cat# 11-004-760-001). Generally ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized with 12 μL of RNA and anchored oligo(dT)18 primer in a 20 μL reaction using the Transcriptor First Strand cDNA synthesis kit at 55°C for 30 min for reverse transcriptase reaction (Roche Diagnostics).
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bioRxiv - Microbiology 2020Quote: ... One μg of intact total RNA per condition was used to make stranded mRNA-seq libraries with the Stranded mRNA-Seq kit (KAPA Biosystems) as per manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... The concentration of each library was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument ...
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bioRxiv - Microbiology 2020Quote: ... free of contaminating DNA) were directed for library preparation using Kapa Stranded mRNA Library Preparation Kit (Roche Diagnostics Corporation, Indiana, USA) and sequencing in an Illumina HiSeq 4000 platform ...
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bioRxiv - Developmental Biology 2020Quote: ... In vitro transcription was performed on the pcr product with Ampliscribe T7-flash kit with Dig RNA labeling mix from Roche(75). In situ hybridization with both the anti-sense probes yielded the same results.
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bioRxiv - Microbiology 2021Quote: ... The cDNA libraries were quantitated using qPCR in a Roche LightCycler 480 with the Kapa Biosystems kit (Kapa Biosystems, Woburn, Massachusetts) prior to cluster generation ...
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bioRxiv - Molecular Biology 2020Quote: ... A particular tRNA was hybridized with a corresponding antisense oligo DNA probe shown in Table S1 labeled with digoxigenin (DIG) using DIG Oligonucleotide Tailing Kit (Roche Diagnostics) in Hybridization Solution [0.50 M Na2HPO4 ...