Labshake search
Citations for Roche :
4801 - 4850 of 5245 citations for Human Cholecystokinin 12 CCK12 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Automated dual indexed FFPE libraries were constructed with 50-250 ng of genomic DNA utilizing the KAPA HTP Library Kit (KAPA Biosystems) on the SciClone NGS instrument (Perkin Elmer ...
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bioRxiv - Neuroscience 2023Quote: ... All purified nucRNA (9-20 ng/sample) was used to construct cDNA sequencing libraries using the KAPA RNA HyperPrep Kit with RiboErase (KK8560, KAPA Biosystems), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: The cellular metabolic viability was measured by NADH-dependent mitochondrial dehydrogenase activity using Cell Proliferation Kit II (XTT) (Roche, Basel, Switzerland) according to the manufacturer’s protocol by measuring the absorbance at 492nm in BioTek Synergy HTX multimode reader ...
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bioRxiv - Zoology 2023Quote: The PCR products amplified from both IHHNV-309 and IHHNV-389 methods were used as templates to prepare digoxigenin (DIG)-labeled IHHNV probes using a PCR DIG-labeling kit (Roche, Germany). After preparation ...
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bioRxiv - Developmental Biology 2023Quote: TUNEL staining was performed according to the manufacturer’s protocol with minor modifications (In Situ Cell Death Detection Kit, TMR red; catalogue number 12156792910; Roche, Mannheim, Germany). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... The RNA was then extracted with the Viral NA Small volume kit (06 543 588 001, Roche Diagnostics GmbH (Mannheim, Germany)) on the MagNA Pure 96 system (Roche Diagnostics GmbH (Manheim ...
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bioRxiv - Neuroscience 2023Quote: A total of 1.0 µg of extracted DNA was used for PCR-free library construction using the KAPA HyperPrep PCR-Free Library Prep kit (Roche, KK8505). Mechanical shearing using the Covaris microtube system (Covaris ...
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bioRxiv - Microbiology 2023Quote: ... Library prep included fragment end treatment, A-tailing, and ligation of Illumina-compatible adapters (IDT, Inc.) using the Kapa-HyperPrep kit (Kapa Biosystems). DNA was then sequenced 2 x 150 bp on the Illumina NovaSeq 6000 platform using S4 flow cells ...
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bioRxiv - Genomics 2023Quote: ... Final library concentration was quantified using size distribution by the Agilent 2200 Tape Station and/or qPCR using the Library Quantification Kit (KK4854, Kapa Biosystems). Equimolar amounts of each library were pooled prior to multiplex sequencing ...
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bioRxiv - Genomics 2023Quote: ... Reverse transcription and second strand synthesis was performed on the slide with cDNA quantification using qRT-PCR using KAPA SYBR FAST-qPCR kit (KAPA Biosystems) and analysed on the QuantStudio (ThermoFisher) ...
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bioRxiv - Immunology 2023Quote: ... Wells were pre-coated with 100 μL per well of capture anti-histone antibody contained in the Cell Death Detection ELISA kit (1:40 in 1x coating buffer; Roche 11544675001) or anti-myeloperoxidase (MPO ...
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bioRxiv - Microbiology 2023Quote: RNA from specimens from Cyprus underwent automated total nucleic acid extraction using the MagNA Pure 96 DNA AND Viral NA Small Volume kit (Roche Diagnostics). RT-PCR for FCoV was performed at Laboklin Bad Kissingen ...
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bioRxiv - Microbiology 2023Quote: ... Indexed libraries that meet appropriate cut-offs for both are quantified by qRT-PCR using a commercially available kit (KAPA Biosystems) and insert size distribution determined with the LabChip GX or Agilent Bioanalyzer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries were quantified using the Qubit BR dsDNA assay kit and equimolar pools were quantified by real-time PCR against a standard curve using the KAPA Library Quantification Kit (Kapa Biosystems). Libraries were sequenced on the NovaSeq 6000 Illumina platform in 150PE mode ...
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bioRxiv - Cell Biology 2023Quote: Libraries were pooled in equimolar amounts and the final library pool was quantified using the KAPA Library Quantification Kit for Illumina Platforms (Roche; KK4824). Libraries were sequenced on the Illumina NextSeq 500 over two NextSeq 500 High Output v2.5 150 cycle kits (Illumina ...
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bioRxiv - Cell Biology 2023Quote: The tissues from treated mice and HFs were used for TUNEL apoptotic detection (In Situ Cell Death Detection Kit, TMR Red, Roche 12156792910) After fixation and antigen retrieval procedures ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 µg of RNA from each sample was processed using the KAPA Stranded mRNA-Seq Kit with mRNA Capture Beads (Kapa Biosystems). Sequencing was performed using NextSeq 500 (Illumina) ...
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bioRxiv - Neuroscience 2023Quote: ... The concentration of each library was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument ...
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bioRxiv - Cell Biology 2023Quote: DNA was extracted from a cell suspension containing approximately 5x 105 cell/ ml using MagNA Pure 96 DNA and Viral NA Small Volume Kit in Magna pure 96 as instructed by the manufacture (Roche, Germany). DNA concentration was measured by NanoDrop 2000 instrument (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... genomic DNA was fragmented using a Covaris S220 ultrasonicator and libraries were generated using KAPA LTP Library Preparation Kit (Roche, KK8230). To verify that the insertion was present in the genome at the correct location in both transfected lines ...
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bioRxiv - Cell Biology 2023Quote: ... 5 ng of DNA from each sample was analyzed using the Kapa Cyber Fast Q-PCR Kit (Kapa Biosystems, Wilmington, MA). The following rDNA primers (designed using Primer3Plus software ...
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bioRxiv - Neuroscience 2024Quote: ... and fluorescein (FITC)-labeled mRNA against Dbh using reverse-transcribed mouse cDNA as a template and an RNA DIG or FITC-labeling kits from Roche (Sigma). Primer and probe sequences for the Slc17a7 (Vglut1) ...
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bioRxiv - Neuroscience 2024Quote: ... Insert sizes were evaluated via Caliper GX (Perkin-Elmer, USA) and libraries quantitated with Kapa SYBR FAST Universal kit (Roche, USA). Sequencing was performed on the Illumina NovaSeq 6000 platform to generate 100M paired reads per sample at 150 nucleotide read lengths ...
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bioRxiv - Genomics 2024Quote: ... Final library quality was assayed using instrumentation described above as well as qPCR using the Kapa Library quantification kit for Illumina (Roche Inc.), before sequencing with other libraries on a quarter Illumina Novaseq S4 flowcell with 2*150 bp paired-end mode at the Norwegian Sequencing Centre.
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bioRxiv - Cancer Biology 2024Quote: ... all extracted genomes were used as the PCR templates and the sgRNA-coding sequences with iBAR were amplified using KAPA HiFi HotStart ReadyMix PCR kit (Roche, #KK2631). The DNA amplification was performed under the following condition ...
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bioRxiv - Cancer Biology 2024Quote: ... The number of PCR cycle used for library amplification was determined by KAPA library quantification kit for Illumina platform (KAPA biosystems). Each Hi-C library was sequenced to obtain 500M reads PE100 with biological duplicates to achieve a total of 1 B reads for each condition.
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bioRxiv - Molecular Biology 2024Quote: ... activity was measured in a kinetic assay,39 using a commercial kit (Sentinel Diagnostics 17234H) adapted for use on either a Cobas Fara or Mira analyser (Roche Diagnostics). Intra-assay and inter-assay precision were CV < 4% and CV < 8% respectively.
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bioRxiv - Microbiology 2024Quote: ... library preparation for metagenomic analysis was conducted using the KAPA Hyper PCR-free library preparation kit (Kapa Biosystems, Roche, Basel, Switzerland), following the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2024Quote: ... library preparation for metagenomic analysis was conducted using the KAPA Hyper PCR-free library preparation kit (Kapa Biosystems, Roche, Basel, Switzerland), following the manufacturer’s guidelines ...
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bioRxiv - Immunology 2024Quote: ... The concentration of each library was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument ...
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bioRxiv - Microbiology 2024Quote: ... Plasma creatinine was measured in freshly thawed plasma samples using the CREP2 kit (Catalog Number 03263991190) on a Cobas 311 analyser (Roche, USA). Assays were performed by a commercial laboratory in the UK ...
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bioRxiv - Molecular Biology 2024Quote: ... Real-time PCR was performed to assess the concentration of target rRNA using SYBR Green Master Mix kit (Roche, Basel, Switzerland) in accordance with the manufacturer’s recommendations on LightCycler® 96 Real-Time PCR System (Roche ...
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bioRxiv - Microbiology 2024Quote: ... mixed with 15 μL of a PCR mix with 1X LightCycler FastStart DNA Master Hybridisation Probes kit (Roche diagnostics, Mannheim, Germany), 0.5 U of UDG (Roche Diagnostics ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries for RNAseq were prepared for each sample with a KAPA stranded mRNA-seq kit as per manufacturer’s recommendations (Roche, Indianapolis, IN); briefly ...
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bioRxiv - Genomics 2024Quote: ... and then by real-time PCR against a standard curve using the KAPA Library Quantification Kit (Kapa Biosystems/Roche, Basel, Switzerland). Libraries were sequenced on an Illumina NovaSeq 6000 platform with S1 ...
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bioRxiv - Genomics 2024Quote: ... and then by real-time PCR against a standard curve using the KAPA Library Quantification Kit (Kapa Biosystems/Roche, Basel, Switzerland). Libraries were sequenced on an Illumina NovaSeq 6000 platform with S1 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and amplified 6 times (Sets 1 and 2) or 8 times (Set 3) with a KAPA Library Amplification Kit (KAPA Biosystems). The amplification products were cleaned using Agencourt Ampure XP reagent or KAPA Pure Beads ...
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bioRxiv - Genetics 2024Quote: ... along with a single -RT sample for each corresponding biological replicate using 2 μl cDNA and a no template control (NTC) with the LightCycler® 480 SYBR Green I Master kit (Roche). Ct values were measured with the Bio-Rad C1000 CFX96 Real-Time System and Bio-Rad CFX Software (qPCR primers ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were pooled in equimolar amounts and final library quantification performed using the KAPA Library Quantification Kit for Illumina (Roche; KK4824).
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bioRxiv - Molecular Biology 2024Quote: ... and an equimolecular pool of libraries was titrated by quantitative PCR using the “Kapa-SYBR FAST qPCR kit for LightCycler480” (Kapa BioSystems) and a reference standard for quantification ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was quantified and poly(A) selected RNA-seq libraries were generated using a KAPA Stranded mRNA-seq kit (KAPA Biosystems) with 500 ng RNA per sample as starting material ...
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bioRxiv - Cancer Biology 2024Quote: ... Relative gene expression was determined using the Quanta SYBR Green Master Mix Kit (Quanta Bio) on a LightCycler 96 (Roche Diagnostics), using GAPDH as the reference gene.
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bioRxiv - Genetics 2023Quote: ... qPCR was performed in a final volume of 20 μl containing 20 ng of total DNA using SYBR Fast Universal qPCR Kit (Kapa Biosystems) and analysed using the Quant Studio 6 Flex system (Applied Biosystems) ...
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bioRxiv - Cell Biology 2024Quote: ... Digoxigenin (DIG)-labeled anti-sense mak RNA probe was generated by in vitro transcription from this T7 promoter: mak cDNA fragment as the template using a DIG RNA labeling kit (Roche, 11175025910). Zebrafish embryos from the 1-cell stage to 72 hpf were fixed with 4% PFA at 4°C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... samples were covered with the staining mixture at 37°C for 1 h following instructions of the In Situ Cell Death Detection Kit (Roche, 11684795910). After the TUNEL reaction ...
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bioRxiv - Cancer Biology 2021Quote: ... size and concentration of cDNA libraries were checked using the Standard Sensitivity NGS kit Fragment Analyzer and qPCR (ROCHE Light Cycler 480). Libraries were sequenced using an Illumina Hiseq 2500 sequencer as single-end 125 base reads ...
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bioRxiv - Cell Biology 2019Quote: ... with ATP standard solutions used at concentrations between 10−8 and 10−5 M (luciferin/luciferase ATP bioluminescence assay kit CLSII, Roche, Basel, Switzerland). Data are expressed as nmol ATP produced/min/106 cells.
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bioRxiv - Cell Biology 2019Quote: ... Quantitative real time polymerase chain reactions (qRT-PCR) were performed in 20 μL reactions using the FastStart universal SYBR Green master (Rox) kit (Roche diagnostics Corp.) on an Mx3000P real-time PCR machine (Agilent Technologies Inc. ...
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bioRxiv - Cell Biology 2020Quote: ... 4 µg of total RNA was used to generate libraries using of stranded poly(A) mRNA-Seq library with the KAPA stranded mRNA Library prep kit (KAPA Biosystems, KK8421). Libraries concentration was quantified with Qubit dsDNA HS (High Sensitivity ...
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bioRxiv - Microbiology 2019Quote: ... separated RNA was transferred to positively charged nylon membranes using overnight capillary transfer in 20X saline-sodium citrate (SSC) buffer as described in the DIG Northern Starter Kit manual (Roche, Cat. 12039672910).